Risa Nofiani
Department of Chemistry, Mathematics and Natural Sciences Faculty, Tanjungpura University, Jl. A. Yani, Pontianak, Kalimantan Barat, Indonesia.

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Antimicrobial, Antioxidant, Hemolytic Activities and Toxicity of Ethyl Acetate Extract From an Unidentified Coral-Associated Fungus, Aspergillus brevipes RK06 Nofiani, Risa; Kurniadi, Rio; Ardiningsih, Puji
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Marine fungi are one of the potential  and prolific sources to produce unique and novel structure of bioactive compounds. The aim of this research was to explore the biological activities potency from ethyl acetate extract of Aspergillus brevipes  RK06. A. brevipes  RK06 was successfully isolated from an unidentified coral from Randayan Island, Kalimantan Barat. The  extract inhibited the oxidation of linoleic acid (Ferric thiocyanate assay) with a lipid peroxidation inhibition value of 28.44%. The IC50 value of the extract for brine shrimp lethality test was 34.19ug/mL. The hemolytic percentage of the extract for hemolysis on cow erythrocytes was 5.21%. The extract showed a growth inhibition against  Klebsiella pneumonia,  Pseudomonas aeruginosa, and Staphylococcus aureus. Based on the assays, the extract showed a potential citotoxity and both low antioxidant and hemolytic activities.   Key words: antioxidant, hemolytic activity, antimicrobial, toxicity,  Aspergillus brevipes RK06
URGENSI DAN MEKANISME BIOSINTESIS METABOLIT SEKUNDER MIKROBA LAUT Nofiani, Risa
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.153 KB) | DOI: 10.31258/jnat.10.2.120-125

Abstract

Marine microorganism is one of biologically active potential resources of secondary metabolites. Its potency areso promising that the knowledge of how its secondary metabolite occured need to be studied and collected. Thoseknowledges will enable further study is improving secondary metabolite production in the laboratory. In nature,secondary metabolites synthesis occur when there are effect of both biotic and abiotic factors such as sea waterand microbe symbiosis with other living materials. When this is explained in metabolic pathways, secondarymetabolite synthesis affected by available nutrient and regulated by autoinducer molecules through quorum sensingmechanism
CHEMICAL AND MICROBIOLOGICAL PROPERTIES OF BUDUK, A COMMERCIAL FISH SAUCE FROM WEST KALIMANTAN Nofiani, Risa; Elminah, Endang; Ardiningsih, Puji
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 3 (2019): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281 KB) | DOI: 10.17844/jphpi.v22i3.29230

Abstract

Buduk is a fish sauce prepared by fermenting fresh small fish with various ingredients such as sugar,roasted rice, vinegar or wine. This study was aimed to assess the physicochemical and microbiologicalproperties of commercial buduk in the Province of West Kalimantan. The Buduk had pH 5-55, moisture52.35%, salt 1.96%, titrated acid 10.80%, free amino nitrogen 1.83 mmol/g, free fatty acid 17.96%, ethanol0.95%, total of mesophilic bacteria 4.81 log CFU/g and lactic acid bacteria 2.09 CFU/g, but no pathogenicbacteria detected. Each commercial buduk showed different physicochemical properties probably causedby variation in recipe of each producer. The microbiological properties did not significantly different but theTMAB found more than the threshold value indicating
ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT Nofiani, Risa; Nurbetty, Siti; Sapar, Ajuk
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 1 No. 2 (2009): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.79 KB) | DOI: 10.29244/jitkt.v1i2.7871

Abstract

The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 ?g/well for S. aureus, 950 ?g/well for Salmonella sp.and 800 ?g/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 ?g/well for S. aureus, 1,050 ?g/well for Salmonella sp., 750 ?g/well for Bacillus subtilis, 350 ?g/well for P. aeruginosa, 750 ?g/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity. Keywords:Antimicrobial,Sponges associated bacteria,MICs
Antimicrobial, Antioxidant, Hemolytic Activities and Toxicity of Ethyl Acetate Extract From an Unidentified Coral-Associated Fungus, Aspergillus brevipes RK06 Nofiani, Risa; Kurniadi, Rio; Ardiningsih, Puji
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss2pp212-216

Abstract

Marine fungi are one of the potential and prolific sources to produce unique and novel structure of bioactive compounds. The aim of this research was to explore the biological activities potency from ethyl acetate extract of Aspergillus brevipes RK06. A. brevipes RK06 was successfully isolated from an unidentified coral from Randayan Island, Kalimantan Barat. The extract inhibited the oxidation of linoleic acid (Ferric thiocyanate assay) with a lipid peroxidation inhibition value of 28.44%. The IC50 value of the extract for brine shrimp lethality test was 34.19ug/mL. The hemolytic percentage of the extract for hemolysis on cow erythrocytes was 5.21%. The extract showed a growth inhibition against Klebsiella pneumonia, Pseudomonas aeruginosa, and Staphylococcus aureus. Based on the assays, the extract showed a potential citotoxity and both low antioxidant and hemolytic activities.Keywords: antioxidant, hemolytic activity, antimicrobial, toxicity, Aspergillus brevipes RK06
PHYSICOCHEMICAL AND MICROBIOLOGICAL PROFILES OF COMMERCIAL CINCALOK FROM WEST KALIMANTAN Nofiani, Risa; Ardiningsih, Puji
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 21 No. 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.304 KB) | DOI: 10.17844/jphpi.v21i2.22851

Abstract

Cincalok, a traditional fermented shrimp product, is prepared with mix small and fresh shrimp, salt, and sugar in a certain ratio incubated for 3-7 days. Different recipes of each the commercial cincalok products obtain different of quality and safety level. The aim of this study is to assess the quality and safety of the commercial cincalok products of West Kalimantan based on their physicochemical and microbiological properties. Seven commercial cincalok products collected from the traditional market of West Kalimantan were analyzed physicochemical (moisture content, pH, free amino nitrogen (FAN), titratable acidity, salt content, glucose content, sucrose content, ethanol content) and microbiological (total number of mesophilic aerobic bacteria (TMABs), total halotolerant bacteria (THBs), endospores bacteria, lactic acid bacteria (LABs), fungi, Enterobacteriaceae, Bacillus cereus, Clostridium perfringens, and Staphylococcus aureus)properties. The mean of moisture content, pH, free amino nitrogen (FAN), titratable acidity, salt content, glucose content, sucrose content, ethanol content for 7 samples was 67.59%, 5.16, 0.60 mM/g, 1.75%, 10.56%,1.30%, 0.49% and 0.59%, respectively. The mean of TMABs, THBs, endospores bacteria, LABs, fungi, and Enterobacteriaceae for 7 samples was 1.19-5.18 log CFU/g and 1.76 log CFU/g, respectively, while B. cereus,C. perfringens, and S. aureus were not detected for all of the samples. The results showed high variation of physicochemical and microbiological properties of the products. Some of the products are safe to ate withoutcooked but the other still contain pathogenic bacteria such as some of the enterobacteriaceae strains.
CHARACTERISTICS OF ANTIMICROBIAL ACTIVITY OF EUCHEUMA COTTONII DOTY-ASSOCIATED BACTERIA EXTRACTS Nofiani, Risa; Kadarisno, .; Daryati, .; Sapar, Ajuk
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 12 No. 2 (2009): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.983 KB) | DOI: 10.17844/jphpi.v12i2.876

Abstract

Marine organisms-associated bacteria have a potential source of antimicrobial compounds. The aim of the research was to find out characteristics of antimicrobial activity of E. cottonii Doty-associated bacteria extracts and their genus from Lemukutan Island, Kalimantan Barat. Five of 11 isolates showed antimicrobial activity namely Flavobacterium sp. K08, Alteromonas sp. K09, Bacillus sp. K10, Klebsiella sp. K11, and Pseudomonas sp. K12. Furthermore, their secondary metabolites were extracted with methanol. The characteristic of the best bacteria extracts minimum inhibitory concentrations (MICs) for Escherichia coli, Bacillus spaericus, Staphylococcus epidermidis, and Candida tropicalis were obtained from bacteria extract of Bacillus sp. K10 (250 ?g/disc), Bacillus sp. K10 (750 ?g/disc), Pseudomonas sp. K12, (500 ?g/disc), and Alteromonas sp. K09 (1,500 ?g/disc) respectively.Keywords: antimicrobial activity, Alteromonas sp., E. cottonii Doty, Flavobacterium sp., Pseudomonas sp.
Proximate Compositions and Biological Activities of Caulerpa lentillifera Nofiani, Risa; Hertanto, Sigit; Zaharah, Titin Anita; Gafur, Sutarman
Molekul Vol 13, No 2 (2018)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.123 KB) | DOI: 10.20884/1.jm.2018.13.2.441

Abstract

Caulerpa lentillifera is an edible and functional seaweed due to its high nutritional compositions and its biological activities.  In this study, C. lentillifera was evaluated for its proximate compositions (moisture, ash, protein, lipid and fiber contents) and its biological activities (antimicrobial, anti-oxidant, and toxicity). Moisture content, crude lipid, crude protein, and crude fiber were determined using oven method, soxhlet extraction, semi-micro Kjeldhal, and hydrolysis, respectively.  Fresh C. lentillifera of Natuna Island, Indonesia, showed its higher level content of ash, crude lipid, and crude fiber compared to that of fresh C. lentillifera of Penghu, Taiwan.  For its biological activity assays, the extracts were prepared from fresh and dry C. lentillifera (FC and DC).  Both of the extracts showed the broad spectrum of weak antimicrobial using well-diffusion agar tests and antioxidant activities using a modified linoleic acid emulsion system.  The toxicity for both extracts was determined using brine shrimp lethality test.  DC extract showed its very low toxicity level and there was no toxicity for FC.  Hemolytic activity was determined using red blood assay.  Both extracts showed their low hemolytic activities (about 5-13%) for the concentration of 100 and 150 ?g/mL, but the activity increased sharply (about 96%) on the concentration of 200 ?g/mL.  It was concluded that C. lentillifera has a potency as a functional food due to containing secondary metabolites with various biological activities.