Articles

ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

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Abstract

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.
PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1434.143 KB) | DOI: 10.20473/ijtid.v2i1.94

Abstract

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.
PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.43 KB) | DOI: 10.20473/ijtid.v3i2.216

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
THE DIFFERENCE OF MAP1LC3 LEVEL AS MACROPHAGE AUTOPHAGY MARKER BETWEEN RESISTANT AND SENSITIVE TUBERCULOSIS PATIENTS ON RIFAMPICIN Wulandari, Dian Novita; Nugraha, Jusak; Soedarsono, Soedarsono
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 1 (2018)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.587 KB) | DOI: 10.20473/ijtid.v7i1.6024

Abstract

M. tuberculosis (MTB) is an intracelular bacteria that live in the host macrophage cells. Several organs can be affected by tuberculosis but most major illnesses are lung diseases. Immediately after infection, MTB will be phagocytosed by the alveolar macrophage cells and can survive in the phagosome. The macrophage plays a role in innate immunity towards an infection using autophagy by removing the microbe directly via phagocytosis. When bacteria phagocytosized, vacuole membrane formed double membranes called autophagosome, and followed by degradation by lysosome, which known as  autolysosome. Induction of autophagy can be observed on the formation of microtubule-associated proteins 1B lightchain 3B (MAP1LC3B/LC3). MAP1LC3B is protein that have role at autophagic way for selection autophagy substrate and biogenesis. In this study we are used serum from patients TB with rifampicin resistant and rifampicin sensitive as control. Samples were divided using gene expert to differentiate between resistant and sensitive rifampicin.This research aims to compare MAP1LC3B levels in resistant and sensitive rifampicin to study macrophages respond in autophagic way in tuberculosis patients, and give information for define therapy plan to improve therapy for MDR-TB patients. Type of this research is a case control study design with cross sectional research with each groups sample is 19 from age 18-65 years old. Result, MAP1LC3B serum levels on the rifampicin resistant group are lower compared to rifampicin sensitive group. This occur because MTB is able to hide and evade innate immune defense mechanisms. MTB can maintain intracellular growth inside the phagosome by inhibiting phagolysosome formation in autophagy process especially inhibit MAP1LC3B formation by PDIM. 
CORRELATION BETWEEN THE USE OF ALBUMIN INFUSION AND CONCENTRATIONS OF SERUM ALBUMIN, PROINFLAMMATORY CYTOKINES (TNF-, IL6) AND SOFA SCORE IN SEPTIC PATIENTS Utariani, Arie; Prasetyo, Budi; Nugraha, Jusak
Folia Medica Indonesiana Vol 52, No 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.452 KB) | DOI: 10.20473/fmi.v52i4.5481

Abstract

Sepsis is a major problem causing high morbidity and mortality in patients who hospitalized in intensive care. In sepsis, there is a change in the distribution of albumin in the intravascular and extravascular compartment, so hipoalbumin is occured. Using albumin in septic patients from several studies that have been done are still controversial. Analyzing albumin infusion with changes in levels of albumin, cytokines and SOFA score in septic patients in intensive care dr. Soetomo. The design of this study is a prospective longitudinal observational. The study was conducted in the intensive care unit or ICU dr. Soetomo in February 2015. The inclusion and exclusion criteria were determined that obtained 15 research subjects. They were examined with serum albumin and proinflammatory cytokines as well as evaluating the SOFA score. Then, it measure with ANOVA test methods. Infusion of albumin positively correlated with increased levels of serum albumin and a negative correlation with the levels of IL6, TNF-a and SOFA score. It also found a correlation between changes in levels of albumin with changing levels of IL6 (p <0:01) and changes in SOFA score (p <0.05).
OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS Nugraha, Hans Kristian; Hernaningsih, Yetti; Nugraha, Jusak
Folia Medica Indonesiana Vol 51, No 3 (2015): July - September 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.569 KB) | DOI: 10.20473/fmi.v51i3.2832

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literature is a double centrifugation method. The purpose of this research is to produce an optimization of the double centrifugation method. This study uses experimental data obtained by researchers at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments generally were conducted on stored blood obtained from the blood bag from PMI with CPD anticoagulant. Optimization of double centrifugation method to produce PRP with optimum platelet count could be obtained by using an "I"-shaped tube as well as other specialized equipment which are currently not available in standard clinical pathology laboratory, and also fresh blood. The resulting increase amounted to 4.11 times with the platelet count of 1.152 million.
POTENSI EKSTRAK Jatropha multifida TERHADAP EKSPRESI VEGF APHTHOUS ULCER Rat norvegicus Wijayanti, Ch. Destri Wiwis; Sudiana, I Ketut; Nugraha, Jusak
Jurnal SainHealth Vol 1, No 2 (2017): September 2017
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

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Abstract

Aphthous ulcer is the most common oral mucosal disease occurring in up to 25% of the worlds human population. In general, therapy is more directed to pain control and hope of faster healing. Increased use of the drug with the original content on the grounds more free toxic effects, easy to obtain and affordable prices cause the use of plants as ingredients of medicine to be an alternative choice. Jatropha multifida known as betadin plant is a plant with bioactive ingredients flavonoids, diterpenoid, tannins and saponins have been widely used as medicinal plants.Research on aphthous ulcer in mouth mucosal of Rat norvegicus was made by using heated burnisher and using 54 Rat norvegicus rats divided into five groups were normal group, K0 (CMC-Na3%), P1 (2.5% JM); P2 (5% JM) and P3 (JM 10%). Giving a topical extract of J.multifida done 2 times a day then sacrificed on day 3 and day 5. VEGF expression in aphthous ulcer in the mouth tissue by immunohistochemical staining. The result of statistical analysis on VEGF expression showed significant difference (p
RESPONS SITOKIN TNF-Α DAN IL-4 PASCA STIMULASI ANTIGEN FUSI RESAT-6-CFP-10 Pratiwi, Rahma Indah; Nugraha, Jusak; Tambunan, Betty Agustina; Tanoerahardjo, Francisca Srioetami
Buletin Penelitian Kesehatan Vol 46 No 1 (2018)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.136 KB) | DOI: 10.22435/bpk.v46i1.57

Abstract

Abstract Protective immunity of tuberculosis (TB) infection is highly dependent on the balance of Th1 and Th2 cytokines. TNF-? cytokines produced by Th1 cell retain a latent status, and IL-4 produced by Th2 aids in the production of antibodies. The recent development of the vaccine candidates shows that rESAT-6- CFP-10 fusion antigen is specific to induce protective immune responses. The objective of the study was to determine the immune response. This study used a quasi experimental design in the laboratory in vitro with cultured PBMC of patients with new cases of pulmonary TB, latent TB and healthy individuals. Examination of TNF-? and IL-4 levels was done by ELISA. The highest TNF-? mean levels were 866,05 in the latent TB group, compared to 814,56 in active TB and 414,58 in healthy individuals, but they were not significantly different. The highest IL-4 mean levels were 1,39 in the active TB group, compared to 0,88 in latent TB and 0,74 in healthy individuals, but they were not significantly different. High levels of TNF-? and low levels of Il-4 in latent TB post-stimulation of rESAT-6-CFP-10 fusion antigen show that the candidate vaccine is capable of providing protective reponse against Mycobacterium tuberculosis infection. Key words : TNF-?, IL-4, PBMC, ELISA, rESAT-6-CFP-10 Abstrak Imunitas protektif terhadap infeksi tuberculosis sangat bergantung terhadap keseimbangan sitokin T-helper (Th)-1 dan Th2. Sitokin TNF-? yang disekresi oleh sel Th1 mampu mempertahankan status laten, dan IL-4 yang disekresi oleh Th2 membantu produksi antibodi. Pengembangan kandidat vaksin terbaru yaitu antigen fusi rESAT-6-CFP-10 bersifat spesifik terhadap respons imun protektif. Tujuan penelitian ini untuk mengetahui respons imun seluler melalui kadar TNF-? dan IL-4 pasca stimulasi antigen fusi rESAT-6-CFP-10. Penelitian ini menggunakan desain eksperimen semu di laboratorium secara in vitro pada kultur PBMC. Pemeriksaan kadar sitokin TNF-? dan IL-4 dengan metode ELISA. Rerata kadar TNF-? pasca stimulasi paling tinggi ditemukan pada kelompok TB laten 866,05, dibandingkan TB aktif 814,56 dan orang sehat 414,58, tetapi tidak berbeda bermakna. Reratakadar IL-4 pasca stimulasi paling tinggi ditemukan pada kelompok TB aktif, dibandingkan TB laten dan orang sehat, tetapi tidak berbeda bermakna. Kadar TNF-? yang tinggi dan kadar IL-4 yang rendah pada TB laten pasca stimulasi antigen fusi rESAT-6-CFP-10 menunjukkan bahwa kandidat vaksin mampu memberikan repons protektif terhadap infeksi Mycobacterium tuberculosis secara invitro. Kata kunci : TNF-?, IL-4, PBMC, ELISA, rESAT-6-CFP-10.
The Examination of ESAT-6, CFP-10, MPT-64 Antigens of Mycobacterium tuberculosis in Urine of Pediatric Tuberculosis Patient with Immunochromatography to Support the Diagnosis Budiarti, Anita; Nugraha, Jusak; Wahyu W, Agung Dwi
Folia Medica Indonesiana Vol 54, No 3 (2018): September
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v54i3.10014

Abstract

Tuberculosis in children is often a systemic complication because of their imperfect cellular immune system. ESAT-6, CFP-10, and MPT-64 are small molecular dominant antigens secreted by Mtb into the blood and filtered by the kidneys into urine. The high proportion of children with TB cases according to the Ministry of Health of the Republic of Indonesia in 2013 was 7.9%-12%. Diagnis is difficult to establish with sputum and blood samples. Therefore, it is necessary to support the diagnosis using urine samples with ICT rapid test. The purpose of this study was to determine the examination of Mtb ESAT-6, CFP-10, and MPT-64 antigens in the urine of pediatric TB patients with ICT to support the diagnosis. The method used was analytic observation with case control design. The results of the study showed that 32 of the urine of children with TB who were examined with ICT, 22 showed ESAT-6, CFP-10, and MPT-64 positive and 10 negative. Whereas, from 32 urine of healthy children, 25 were negative and 7 were positive. Analysis results showed 95% confidence (CI), p = 0.0002 (p <0.05), 78% specificity and 68.8% sensitivity compared to TB diagnosis from pediatricians. As a conclusion, Mtb ESAT-6, CFP-10, and MPT-64 antigens can be examined in the urine of pediatric TB patients with ICT to support the diagnosis.
Co-Authors Agustina, Betty Aminuddin, M. aminuddin, mohammad Amminuddin, Muhammad Aryati Aryati Beatrix, Stephanie betty Agustina Tambunan, betty Agustina Buana, Dwi Candra Budi Prasetyo Budiarti, Anita Budiutari, Ni Nyoman Clarissa, Sheila Darmadi, Epriyanto T Darwin, Bastian Dominicus Husada, Dominicus Eddy Mart Salim, Eddy Mart Edijanto, SP Elita, Myrza Febtarini Rahmawati Fransiska Fransiska Handayani, Luh Putu Trys Monika Hans Kristian Nugraha, Hans Kristian Hartono Kahar, Hartono Heru Setiawan I Ketut Sudiana Ilham Ilham Indrasari, Yulia Nadar Indrasari, Yulia Nadar John Wiwin, John julious, julious Kasiyati, Menik kurniati, nova Lisa Soegianto Marpaung, Ferdy Royland Marpaung, Holland Lydia Meiti Muljanti, Meiti Mgs Irsan Saleh, Mgs Irsan Muhammad Nazarudin, Muhammad Ninik Asmaningsih Soemyarso Nugraha, Hans K Oetami, Fransiska Sri poespitaningtyas, esthy Pratiwi, Rahma Indah Prihantika S., Sabrina Priyanto, Hery Pudji Lestari Purwanta, Marijam Puspitasari, Yessy rahadiyanto, Kemas Ya'kub Roedi Irawan, Roedi Ruddy, Triagung Ryzky Widi Atmaja, Ryzky Widi Saleh, M Irsan Saputra, Angky Setiawan, Lidwina Tri Kristanti Setyaningrum, Citra Indah Sinansari, Restry Soedarsono Soedarsono Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanoerahardjo, Francisca Srioetami Tobing, Erna R Utariani, Arie Wahyu Dewi Tamayanti, Wahyu Dewi Wahyu W, Agung Dwi Wibowo, Wahyu Setiani Widyadi, Elvan Dwi Wijayanti, Ch. Destri Wiwis Wulandari, Dian Novita Yetti Hernaningsih, Yetti Yoes Prijatna Dachlan Zen Hafy