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Uji Aktivitas Antibakteri Ekstrak Serbuk Gergaji Kayu Eboni (Diospyros celebicaBakh.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Wahyuni, Wahyuni; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Ebony (Diospyros celebica Bakh.) is one of the beneficial plants from ebenaceae, especially in traditional medicine. The sawdust extract containing chemical compounds such as tannins, saponins and terpenoids. This research were aimed to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) also to identified the active compounds from sawdust extracts of D. celebica Bakh. against Staphylococcus aureus and Escherichia coli. The Research methods include extraction using maceration method by ethanol 96%. MIC and MBC determination using dilution and diffusion methods. The Results showed that MIC value of S.aureus and E. coli were 6% and 7%, respectively. While MBC value of S. aureus and E. coli were 12% and 13%, respectively. Identification of chemical compounds were determined by Thin Layer Chromatography (TLC) bioautography method by 10% H2SO4 and anisaldehid-sulfuric acid reagent spray. There were two chemichal compounds that produced, saponin and terpenoid.
STUDI ETNOBOTANI TUMBUHAN BERKHASIAT OBAT PADA SUKU TAU TAA WANA DI DESA BULAN JAYA KECAMATAN AMPANA TETE, KABUPATEN TOJO UNA UNA, PROVINSI SULAWESI TENGAH Hijrah, Hijrah; Nugrahani, Arsa Wahyu; Ramadanil, Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Ethnobotanical study of Tau Taa Wana Tribe aims to determine the types and parts of plants used as traditional medicine as well as knowing how to use medicinal plants by Tau Taa Wana Tribe in the Bulan Jaya Village Ampana Tete District, Tojo Una Una Regency, Central Sulawesi Province. The study was a descriptive research using qualitative methods with the technique of sampling snowball sampling. Information obtained through open-ended interviews at 9 informants interviewed based on the questions that had been developed previously. The results showed that there were 69 species of plants that are divided into 38 families used as medicine. The most widely used plants originate from Asteraceae and Solanaceae family each 5 species. Part used include leaves, stems, fruits, roots, rhizomes, tubers, herbs, seeds, bark and flowers. Percentage of part medicinal plants most widely used are the leaves (32%). The most used method is to be drunk.
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia Zubair, Muhammad Sulaiman; Lallo, Subehan; Rusmiyanti, Rusmiyanti; Nugrahani, Arsa Wahyu; Jantan, Ibrahim
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1388.045 KB) | DOI: 10.14499/indonesianjpharm29iss4pp173

Abstract

Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively.  Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both  extracts suggesting for further purification and isolation for the bioactive terpenoid compounds. 
ETNOBOTANI SUKU TOGIAN DI PULAU MALENGE KECAMATAN TALATAKO, KABUPATEN TOJO UNA-UNA, SULAWESI TENGAH Tabeo, Dalton Fidel; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Ethnobotany study in Togian Tribe has done in Tojo Una-Una, Central Sulawesi. This study was aimed to find out the information about the types of plants, used parts, processing, usage and dosage as well as knowing what type of disease treated using medicinal plants by Togian tribal communities in Malenge Island, Talatako District, Tojo Una- una, Central Sulawesi. This research  was a descriptive study with qualitative methods and used snowball sampling through interviews on four informants with the questionnaire. The results showed that 60 species are divided into 33 families. the most widely used was the Asteraceae by 7 species and Fabaceae familia by 6 species. Plant parts include leaves, stems, fruit, roots, rhizomes, herbs, seeds, sap and flowers partnered with the largest percentage of leaves 56%. the using and processing of medicinal plants by the Togian Tribe Malenge Island were boiled, mashed, squeezed, shredded, soaked, drunk, chewed, dropped, scrubbed, and pasted. the most  using and processing were boiled and drunk.
Uji Aktivitas Antibakteri Formula Pasta Gigi Ekstrak Batang Karui (Harrisonia Perforata Merr.) Terhadap Bakteri Streptococcus Mutans Nurjannah, Wardatul; Yusriadi, Yusriadi; Nugrahani, Arsa Wahyu
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Karui (Harrisonia perforata Merr.) is one of the many species that live in tropical regions such as Central Sulawesi (Palu) and has antibacterial activity. This research aims to find the variation effect of karui stem extract (Harrisonia perforata Merr.) in toothpaste towards stability of physical quality and antibacterial activity bacteria Streptococcus mutans. The concentrations of karui stem extract 1%, 2%, and 3% in toothpaste tested for antibacterial activity using pitting method which further tested for statistical analysis "One Way Anova" using SPSS 16.0 software. The results of the evaluation of toothpaste Karui stem extract F1 (1%), F2 (2%), and F3 (3%) showed that the test of the organoleptic and homogenity has no significant difference during the storage time (28 days) but the test at pH, viscosity, and the scatter has significant difference. The Formula of karui stem ekstract with 3 concentrations F1 (1%), F2 (2%), and F3 (3%) forming an average diameter of antibacterial inhibitory 6.65 mm; 7.14 mm; and 9.85 mm. The results of statistical analyzed from toothpaste test diameter inhibitory zone showed an unsignificant difference. Keywords: toothpaste, extract, karui (Harrisonia perforata Merr.), Streptococcus mutans
STUDI TANAMAN BERKHASIAT OBAT SUKU MORI DI KECAMATAN PETASIA, PETASIA BARAT, DAN PETASIA TIMUR KABUPATEN MOROWALI UTARA SULAWESI TENGAH Idris, Idris; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This study aims to conduct an inventory, knowing how to use and organs of plants used as medicine by Mori Tribal in Petasia, Petasia and East Petasia District, West North Morowali Regency of Central Sulawesi. This study used qualitative methods and sampling techniques that snowball sampling, with open-ended interview techniques interview on media 9 informants obtained using a questionnaire. Based on the results of research known as 79 species of medicinal plants, 2 of which can not be identified and divided into 45 familia used as a medicine. Plants are most widely used is of familia Euphorbiaceae as many as 8 species. Organs of plants used include leaves, stems, fruits, rhizomes, seeds, bark, flowers, bulbs, herbaceous roots and sap. Organ plant is widely used is the leaves that percentage utilization is 58%. Mori Tribal communities in the District, West Petasia and East Petasia North Morowali District use medicinal herbs to treat the disease, healing and health maintenance. The cooking methods include boiling water, brewed, boxed, taped, squeezed, scrubbed/smeared, chewed and consumed directly, drops, and sprayed. How to use include drunk, eaten, chewed and consumed directly, polished/smeared, attached, dropped, and chewed and sprayed on the affected area.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JARAK MERAH (JATROPHA GOSSYPIFOLIA) TERHADAP BAKTERI ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS Torokano, Semuel; Khumaidi, Akhmad; Nugrahani, Arsa Wahyu
Natural Science: Journal of Science and Technology Vol 7, No 1 (2018): Volume 7 Number 1 (March 2018)
Publisher : Univ. Tadulako

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Abstract

dengan Jarak Pagar (Jatropha curcas) yang sebelumnya telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri, konsentrasi optimum antibakteri dan senyawa yang berperan terhadap aktivitas antibakteri dari ekstrak etanol daun jarak merah (Jatropha gossypifolia L.) terhadap Staphylococcus aureus dan Escherichia coli. Ekstraksi senyawa aktif menggunakan metode maserasi dengan pelarut etanol. Pengujian aktivitas antibakteri dilakukan dengan varian konsentrasi 20%, 40%, 60%, 80% dan 100% dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak etanol daun jarak merah memiliki aktivitas antibakteri terhadap            S.aureus dan E.coli dengan konsentrasi optimum penghambat pertumbuhan bakteri yaitu 60%         (S.aureus) dan 80% (E.coli). Berdasarkan pengujian bioautografi dan KLT dengan eluen                   n-heksana:etil asetat (1:2) menggunakan pereaksi kromogenik senyawa yang diduga berperan terhadap aktivitas antibakteri adalah terpenoid
Aktivitas Antibakteri Fraksi Allium ascalonicum Linn A562275sal Lembah Palu terhadap Shigella dysenteriae Khumaidi, Akhmad; Maulina, Kumalahayati; Nugrahani, Arsa Wahyu
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1375.333 KB) | DOI: 10.35814/jifi.v17i2.728

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Bawang merah (Allium ascalonicum Linn.) dari lembah Palu merupakan salah satu tanaman khas Sulawesi Tengah yang digunakan sebagai obat tradisional seperti untuk mengobati penyakit infeksi. Penelitian ini bertujuan untuk mengetahui fraksi yang mempunyai aktivitas tinggi dari sampel umbi dan daun bawang merah dari lembah Palu dalam menghambat pertumbuhan bakteri Shigella dysenteriae dan menentukan golongan senyawa yang memiliki aktivitas tersebut. Penelitian dengan ekstraksi bawang merah menggunakan  metode maserasi dengan etanol 96 % terhadap kedua sampel. Fraksinasi cair-cair digunakan sebagai metode fraksinasi menggunakan pelarut heksan, etil asetat dan air secara berurutan. Uji aktivitas antibakteri menggunakan metode difusi dengan teknik sumuran dan KLT Bioautografi. Hasil penelitian menunjukkan bahwa fraksi etil asetat memberikan aktivitas tertinggi dari sampel umbi, sedangkan pada sampel daun fraksi etil asetat dan fraksi heksana mampu memberikan aktivititas penghambatan yang baik. Golongan senyawa yang teridentifikasi dengan metode kromatografi lapis tipis adalah senyawa flavonoid untuk sampel umbi (Rf 0,18). Pada sampel daun teridentifikasi senyawa steroid pada fraksi etil asetat (Rf 0,62) dan senyawa terpenoid (Rf 0,48) pada fraksi heksana. Berdasarkan hal tersebut Bawang merah dari  lembah Palu berpotensi dapat dikembangkan sebagai bahan antibakteri.
KAJIAN ETNOFARMASI ETNIK BUNGKU DI KECAMATAN BUNGKU TENGAH KABUPATEN MOROWALI PROVINSI SULAWESI TENGAH Hermin, Hermin; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Vol. 2 No. 2 (2016): (October 2016)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.865 KB) | DOI: 10.22487/j24428744.2016.v2.i2.5956

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This study aims to find out and to inventory the types and parts of plants as well as to dig up information about ways of their utilization as remedy used by Bungku people ?To Bungku?. It was conducted from August to December 2015 in Bungku Subdistrict, Central Bungku, Morowali Regency, Central Sulawesi. This research is a descriptive study using qualitative methods and snowball sampling technique on 12 informants through data collection by questionnaire. Results of this etnopharmacy study revealed that there were 62 species of medicinal plants divided into 34 familia. The most widely used plants came from familia Euphorbiaceae, Fabaceae and Zingiberaceae, each was as many as 8%. Parts of plant including roots, stems, leaves, flowers, fruits, seeds and bulbs were used in accordance with their function. Leaves were the ones used with the highest percentage (50%). Bungku people used medicinal plants to treat diseases such as kidney stone, diabetes, hemorrhoids, diarrhea, abscess, candidiasis, gout, cancer, hypertension, hypercholesterolemia, dyspepsia, cough, abdominal pain, malaria, hematuria, skin inflammation, headache, fever, bronchitis, dysentery, asthma, stroke, gallstones, urinary tract infections, broken bones, nail-punctured, wound ulcers, burns, hepatitis, appendicitis. Ways of the utilization were very diverse such as boiling then drinking the decoction; crushing then rubbing on the necessary skin; attaching to the body; squeezing then drinking the juice; and heating on the fire. Processing by boiling has the largest percentage, as much as 54%
PRODUKSI PROTEIN SEL TUNGGAL DARI KULTUR Rhizopus oryzae DENGAN MEDIUM LIMBAH CAIR TAHU Maryana, Leny; Anam, Syariful; Nugrahani, Arsa Wahyu
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Vol. 2 No. 2 (2016): (October 2016)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.442 KB) | DOI: 10.22487/j24428744.2016.v2.i2.5987

Abstract

Tofu wastewater is the origination of tofu?s production process that can degrade the quality of the environment if not handled properly. Another impact of this waste is the potential attack that could evoke the diseases such as diarrhea, skin diseases, and other illnesses for people who use the stream flow that has been polluted by this wastewater. However, the high-carbohydrate and protein of this waste may allow it to be used more as a microbial growth substrate to produce a Single-cell Protein. The purpose of this study is to determine the optimum incubation time and the highest protein content of Rhizopus oryzae in the medium of tofu wastewater based on the calculation of protein content percentage and the analysis of cell dry mass. And to know the optimum pH growth with the variation of pH growth which is about 4, 5, and 6. The calculation is performed in accordance with the fermentation time at 24, 48, and 72 hours. Total protein content calculated by the Kjeldahl method. The results showed the highest protein content obtained in the incubation period to 48 hours with an average percent of protein about 0.47%, 0.47%, 0.46% and a mass of cell with an average value of 0.77%, 0, 84% and 0.91%. The optimum pH of Rhizopus oryzae growth in the medium of tofu wastewater is about 5 pH. It can be concluded that the wastewater of tofu potentially used as a growth substrate of Rhizopus oryzae. Keywords: Wastewater of Tofu, Single-cell Protein, Rhizopus oryzae.