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APLIKASI BERBAGAI MARKA AROMATIK PADA VARIETAS PADI INDONESIA Seno, Djarot Sasongko Hami; Nugroho, Satya; Santoso, Tri Joko; Adrianto, Dimas; Praptiwi, Dewi; Apriana, Aniversari; Mas'ud, Zainal Alim
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young  leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern. 
SELEKSI GENOTIPE PADI MUTAN INSERSI TOLERAN CEKAMAN SALINITAS BERDASARKAN KARAKTER PERTUMBUHAN DAN BIOKIMIA Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (590.044 KB) | DOI: 10.24831/jai.v38i1.1671

Abstract

Eleven genotypes of insertional mutant Nipponbare rice carrying randomly inserted activation tag were tested to investigate the growth and biochemical responses to salt stress. Pokkali, IR 29, and non-transgenic Nipponbare were used as tolerant, susceptible, and isogenic control, respectively. Plants were grown in plastic trays filled with 0  g L-1 NaCl  Yoshida nutrient solution as control and grown in 6 g L-1 NaCl as treatment. Salt injury was scored on 10, 16, and 21 days after salinization. Observation at 21 days after salinization showed that there was significant  difference  among genotypes in growth and biochemical characters. Classification  using hierarchical cluster analysis based on growth and biochemical responses showed that there were three clusters. Cluster I consisted of wild-type Nipponbare, T2.pMO.V.3.13.c.22, T2.pMO. V.1.7.a.15, T2.pMO.III.98.b.7, T2.pMO.II.231.c15, T2.pMO.III.98.b.8, T2.pMO.III.98.b.17, T2.pMO.III.98.b.3, and T2.pMO.VI.81.3.a.4. Cluster II consisted of IR 29, T2.pMO.V.3.13.c.14, T2.pMO.VI.30.1a.125, and T2.pMO.VI.30.1.a.65. However no mutant genotype was clustered with Pokkali in cluster III, which may indicate that there was no salt tolerant mutant genotype found in this research.   Keywords: salt tolerance mutant rice, growth characters, biochemical characters
UJI RADIOSENSITIVITAS SINAR GAMMA UNTUK MENGINDUKSI KERAGAMAN GENETIK SORGUM BERKADAR LIGNIN TINGGI Astuti, Dwi; Sulistyowati, Yuli; Nugroho, Satya
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 15, No 1 (2019): JUNI 2019
Publisher : BATAN

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Abstract

Sorgum (Sorghum bicolor) merupakan tanaman serealia yang dapat ditanam di lahan kering dan cukup potensial untuk dikembangkan di Indonesia. Sorgum dapat dimanfaatkan sebagai bahan pangan, pakan dan industri. Salah satu kandungan dalam sorgum yang dimanfaatkan untuk bahan industri adalah lignin yaitu digunakan dalam pembuatan particle board sehingga dapat mengurangi pemakaian kayu (penebangan pohon). Dalam penelitian ini digunakan 2 genotipe sorgum dengan kandungan lignin tinggi yaitu Konawe Selatan (KS) dan Sorgum Malai Mekar (SMM) untuk ditingkatkan keragaman pada karakter biomasa melalui pemuliaan mutasi induksi. Keberhasilan mutasi iradiasi sinar gamma sangat ditentukan oleh sensitivitas genotipe tanaman. Penelitian ini bertujuan untuk menentukan rentang dosis optimal yang dapat menginduksi variasi genetik untuk genotipe KS dan SMM. Benih sorgum KS dan SMM diiradiasi sinar gamma dosis 0  - 1000 Gy increment 100 Gy. Pengamatan dilakukan terhadap populasi tanaman M1 untuk persentase daya kecambah dan tinggi tanaman pada dua minggu setelah tanam (2 MST). Hasil penelitian menunjukkan bahwa dosis iradiasi menghambat pertumbuhan benih sorgum yang ditunjukkan oleh tanaman kerdil atau tidak berkembang. Rentang dosis optimum yang didapatkan untuk sorgum genotipe KS dan SMM adalah 300 ? 500 Gy.
PENGARUH MODAL, TENAGA KERJA DAN TEKNOLOGI TERHADAP HASIL PRODUKSI SUSU KABUPATEN BOYOLALI Nugroho, Satya; Budianto, Muchamad Joko
JEJAK: Jurnal Ekonomi dan Kebijakan Vol 7, No 2 (2014): September 2014
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jejak.v7i2.3896

Abstract

Currently, most of milk in Indonesia should be imported. It is about 78,89 %, and the rest, that is about 22,11% is from local production or local dairy farmers. However, there are some problems faced by the dairy farmers in Kecamatan Musuk. They are capital, workforces, and technology. This study aims at knowing the influence of capital, workforces, and technology to the milk production in Kecamatan Musuk. Ordinary Least Square (OLS) was applied for analizing the data. The results show that the regression coefficient values of each independent variable (the variables of capital, workforces, and technology) positively influence the production of milk. Based on the T-test (partial), the capital and workforces influence positively and significantly to the production. In addition, technology also has positive effect to the milk production, but it is not significant. Finally, based on F-test, the milk production is influenced by the capital, workforces and technology. It is 87%. Saat ini sebagian besar susu di Indonesia masih harus di impor (sekitar 78,89%), sedangkan 22,11%nya di pasok dari produksi susu domestik yang sebagian besar dihasilkan oleh peternak sapi perah rakyat. Namun ada beberapa permasalaham yang kerap dihadapi oleh peternak sapi perah di Kecamatan Musuk, baik dari segi permodalan, tenaga kerja maupun teknologi yang dipakai.Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh dari penggunaan faktor produksi modal, tenaga kerja dan teknologi terhadap hasil produksi susu sapi perah di Kecamatan Musuk. Alat analisis yang digunakan adalah analisis regresi berganda atau Ordinary Least Square (OLS).Hasil analisis diperoleh bahwa nilai koefisien regresi masing-masing variabel bebas yaitu variabel Modal, Tenaga Kerja, dan Teknologi berpengaruh secara positif terhadap produksi susu sapi perah. Dari hasil uji-t (parsial) modal dan tenaga kerja berpengaruh positif dan signifikan sedangkan teknologi berpengaruh positif namun tidak signifikan. Uji F menunjukkan produksi susu sapi perah dipengaruhi oleh modal, tenaga kerja dan teknologi sebesar 87%.
PENAPISAN BEBERAPA PADI LOKAL DARI PULAU ENGGANO TERHADAP KETAHANAN SALINITAS Astuti, Dwi; Nurhasanah, Ade Nena; Nugroho, Satya; Estiati, Amy
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 6, No 2 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.544 KB) | DOI: 10.33230/JLSO.6.2.2017.282

Abstract

Astuti et al, 2017. Screening of Several Local Rice from Enggano Island to Salinity Tolerance. JLSO 6(2):134-141. Enggano Island, Bengkulu Province, has a lot of local rice germplasm that has been cultivated by people in Enggano Island that is more than 30% as farmer. Identification of local rice from Enggano Island against biotic and abiotic stresses, especially salinity stress is necessary. The identification can be used, among other things, as the information of the local rice planting that related to the rising sea level in Enggano Island which impact on the condition and the area of rice cultivation land, and as a source of finding for resistance genes against salinity stress for improvement of those local rice varieties. Aim of this study is preliminary screening of some local rice from Enggano Island to salinity stress in seed and germination stages. The analysis used Factorial Randomized Design, two factors, the first factor  is salt concentration and and the second is local variety. The salt concentrations used were 0 millimolar NaCl, 250 millimolar NaCl, NaCl 275 millimolar and NaCl 300 millimolar and the rice sample used were 11 local rice of Enggano Island, NiponBare varieties as control of rice plants, Pokali as resistant control and IR29  varieties as susceptible controls. Rice seeds that used in this test are seed in seed stage and germination stage of 10-14 days old. This preliminary screening shows three local genotype of Enggano Island that tolerance to Salinity.
Gene flow from genetically modified rice to their weedy and wild relatives and its environmental consequences Estiati, Amy; Nugroho, Satya
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/32

Abstract

Rice is one of the most important food crops in the world.  Nearly half of the world population consume rice as their staple food (FAO, 2004).  With the increasing of world population, the need to provide more food supplies including rice is obvious.  Biotechnology is expected to play major roles in the improvement of crop productivity and quality. Unlike transgenic maize, eventhough many research have been done to improve rice performance by genetic engineering, transgenic rice has not been released or commercialized.  Among those research to improve rice performance are the atempt to introduce biotic and abiotic stress tolerant traits, herbicide tolerant trait and beta-carotin biosynthetic pathway.  Currently, several genetically modified rice cultivars have been and being tested on limited field trials.Keywords : Rice, stress tolerant
RESPON PERTUMBUHAN PADI MUTAN INSERSI PADA KONDISI NITROGEN RENDAH Nurhasanah, Ade Nena; Nugroho, Satya
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 6, No 1 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.718 KB) | DOI: 10.33230/JLSO.6.1.2017.274

Abstract

Nurhasanah et al, 2017. Response Rice Mutant Insertion Growth in the Low Nitrogen Conditions. JLSO 6(1):49-58.Nitrogen is an essential fertilizer for growing rice because N needed higher than other nutrients. Therefore, many farmers apply a large amount of nitrogen fertilizer to obtain high production. Unfortunately, the plant not utilized much and only 50-60 % of the total N was applied which able to used. It would be lost through a combination of leaching, surface run-off, denitrification, volatilization and microbial consumption. Thus, the large amount N fertilizer application increase input cost and damage the environment. To build sustainable agriculture, the screening of varieties or mutant which is capable of using N efficiently is necessary. In addition, knowledge of N regulation and mechanisms in plants needs to be exploited for efficient plant development in the use of N. This study aims to look at the response of mutant rice plants in the low N conditions and obtain mutant rice plant candidates which is efficiently in using N. Seventen mutan insertion and Niponbare as wildtype are used in this experiment. Rice mutant plants were grown hydroponically inYoshida solution with different N concentration. Under normal conditions the N concentration was 1.14 mM/L and in the low N concentration was about 0.114 mM./L. Parameters was measured in this study were plant height, root length and dry weight. The results showed that plant height mutant insertion wasnot influenced by N concentration in nutrient solution. Meanwhile root lenght and dry weight plant parameter are affected by N consentration. There are mutant insertion plant that have potential and the optimum growth ability at low N concentration, especially B101. Validation on that mutants will be done in the next research.
Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Nugroho, Satya; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/29

Abstract

Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority.  The screening protocol based on PEG 6000 has been developed and was  being used to screen 70 mutant lines to characterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds).  These characters were used to score the Degradation Index  and Vigour Index. Results showed varrying responds of the lines to the osmotic presure.  Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified.  Some inconsistencies in the performances scored by both indices were thought to be due to seed quality.   Keywords:  Oryza sativa, insertion mutant, drought, PEG 6000, Degradation Index, Vigor Index.
PENGARUH MODAL, TENAGA KERJA DAN TEKNOLOGI TERHADAP HASIL PRODUKSI SUSU KABUPATEN BOYOLALI Nugroho, Satya; Budianto, Muchamad Joko
JEJAK: Jurnal Ekonomi dan Kebijakan Vol 7, No 2 (2014): September 2014
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jejak.v7i2.3896

Abstract

Currently, most of milk in Indonesia should be imported. It is about 78,89 %, and the rest, that is about 22,11% is from local production or local dairy farmers. However, there are some problems faced by the dairy farmers in Kecamatan Musuk. They are capital, workforces, and technology. This study aims at knowing the influence of capital, workforces, and technology to the milk production in Kecamatan Musuk. Ordinary Least Square (OLS) was applied for analizing the data. The results show that the regression coefficient values of each independent variable (the variables of capital, workforces, and technology) positively influence the production of milk. Based on the T-test (partial), the capital and workforces influence positively and significantly to the production. In addition, technology also has positive effect to the milk production, but it is not significant. Finally, based on F-test, the milk production is influenced by the capital, workforces and technology. It is 87%.Saat ini sebagian besar susu di Indonesia masih harus di impor (sekitar 78,89%), sedangkan 22,11%nya di pasok dari produksi susu domestik yang sebagian besar dihasilkan oleh peternak sapi perah rakyat. Namun ada beberapa permasalaham yang kerap dihadapi oleh peternak sapi perah di Kecamatan Musuk, baik dari segi permodalan, tenaga kerja maupun teknologi yang dipakai.Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh dari penggunaan faktor produksi modal, tenaga kerja dan teknologi terhadap hasil produksi susu sapi perah di Kecamatan Musuk. Alat analisis yang digunakan adalah analisis regresi berganda atau Ordinary Least Square (OLS).Hasil analisis diperoleh bahwa nilai koefisien regresi masing-masing variabel bebas yaitu variabel Modal, Tenaga Kerja, dan Teknologi berpengaruh secara positif terhadap produksi susu sapi perah. Dari hasil uji-t (parsial) modal dan tenaga kerja berpengaruh positif dan signifikan sedangkan teknologi berpengaruh positif namun tidak signifikan. Uji F menunjukkan produksi susu sapi perah dipengaruhi oleh modal, tenaga kerja dan teknologi sebesar 87%.
Pengembangan Nontransgenik F1 dan Bc1f1 Padi Ciherang Toleran Genangan secara Site-Directed Crossing Hami Seno, Djarot Sasongko; Nugroho, Satya; Santoso, Tri Joko; Sinaga, Joel Rivandi; Marlina, Euis; Adrianto, Dimas; Munzirwan, Rudi; Apriana, Aniversari; Mas'ud, Zainal Alim
Current Biochemistry Vol 1, No 3 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/136-145

Abstract

The development of submergence tolerant rice varieties is urgently required to maintain the stability of future food production, to anticipate the unpredictable global climate changes. Due to in-economical agronomic traits of native submergence tolerant varieties for large scale cultivation, submergence tolerance gene (sub1) must be introduced into popular high-yielding rice variety, such as Ciherang. To develop new submergence tolerant variety with good agronomic traits as those of Ciherang, in this research, submergence tolerance gene (sub1) was introduced into Ciherang variety. To avoid strict GMO regulation, gene introduction was carried out through site-directed crossing. Donor sub1 was crossed with Ciherang host. The selected F1 progenies were further backcrossed to Ciherang 4 x to obtain BC5F1 progeny having ~98% agronomic traits of those of Ciherang. In every cross/backcross generation, submergence test was performed, followed by sub1 marker-assisted PCR. F1 and BC1F1 submergence-tolerant Ciherang were successfully constructed. Co-dominant RM464A marker was not able to discriminate between host, donor, and progenies (F1 and BC1). Co-dominant RM219 maker showed slightly different size between donor and host amplicon, but it was difficult to see their heterozygous progenies. Both C173 and AEX1 dominant markers were able to show sub1 introgression from donor to host. PCR results confirmed that progenies-submergence tolerance was due to sub1 introgression, not escape mechanisms. AEX1 was chosen for subsequent experiments. Backcross until BC5 is in progress, to obtain maximum host retention for engineering new submergence tolerant varieties with good agronomic traits as those of Ciherang.