Widagdo Sri Nugroho
Departemen Kesehatan Masyarakat Veteriner. Fakultas Kedokteran Hewan, Universitas Gadjah Mada

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DETECTION OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS FORMULA MILK IN BOGOR Nugroho, Widagdo Sri; Sudarwanto, Minarwati; Lukman, Denny Widaya; Usleber, Ewald
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn�s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John�s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57
PCS-11 MESENCHYMAL STEM CELL-CONDITIONED MEDIUM IMPROVE THE RECOVERY OF PANCREATIC α AND β CELLS IN TYPE 1 DIABETES MELLITUS Masithoh, Dian Bekti Hadi; Fibrianto, Yuda Heru; Anggita, Marla; Nugroho, Widagdo Sri; Budipitojo, Teguh
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Mesenchymal stem-cell is a pluripotent cells that can be differentiated into many kinds of cells (1) and have abilities to differentiated into mature cell, secreted cytokines, and releasing many kinds of protein functioned in body body regulation (2). However, the therapeutic effect of stem cell arise from their secreted factor such as growth factors, cytokines, chemokines, and metabolits which acts as biologic regulator in the autocrine and paracrine body function (3). Secreted factors of mesenchymal stem cells known as secretome or mesenchimal stem cell conditioned medium (MSC-CM) have therapeutic effect for antiapoptosis, angiogenic, immunomodulatoric, and chemoattraactive activity (4). Mesenchymal stem cell conditioned medium used as the replacement agent for cell therapy because it contains no cells and save for used in different individual (5).Type 1 diabetes mellitus (DMT1) is a disorder characterized by destruction of the insulin-producing pancreatic ?-cell that progressively leads to insulin de?ciency and resultant hyperglycemia. Insulin de?ciency can lead to progressive metabolic derangement, with worsening hyperglycemia, ketoacidosis, starvation, and death (6). Glucagon is a hormone produced by pancreatic ? cells. Glucagon releases by the pancreas to raise the concentration of glucose in the bloodstream when the concentration of glucose in the bloodstream too low (7). Glucagon worked in opposite with insulin, which lowers the extracellular glucose level, while insulin works to increase the extracellular glucose level (8). The aim of this study was to investigate the role of MSC-CM on the structural and functional regeneration of pancreatic ? and ? cells in Wistar rat (Rattus norvegicus) induced with type 1 diabetes mellitus. The datas in this study will completes the information about the effect of MSC-CM on pancreatic cells regeneration, which has been presented in previous study (9).
KADAR MALONDIALDEHID TIKUS DIABETES MELITUS TIPE 2 DENGAN TERAPI EKSTRAK MEDIA PENUMBUH SEL PUNCA MESENKIMAL Fitriana, Ida; Wijayanti, Agustina Dwi; Sari, Puspa Wikan; Satria, Raden Gagak Donny; Setiawan, Dwi Cahyo Budi; Fibrianto, Yuda Heru; Nugroho, Widagdo Sri
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.67 KB) | DOI: 10.29244/avi.5.1.29-36

Abstract

Berbagai penelitian menunjukkan bahwa ekstrak media penumbuh sel punca mesenkimal (EMPSPM), tanpa sel punca itu sendiri, telah ditemukan terdapat berbagai faktor tropik hasil sekresi sel punca mesenkimal di dalam media kultur yang dapat meregenerasi jaringan yang rusak. Penelitian ini bertujuan untuk mengetahui gambaran kadar malondialdehid (MDA) dalam plasma dan ginjal tikus diabetes melitus tipe 2 (DMT2) dengan terapi EMPSPM. Dua puluh lima ekor tikus wistar digunakan dalam penelitian ini dibagi menjadi 5 kelompok yaitu kelompok DMT2 + 0,05 ml/kg BB EMPSPM (0,05); kelompok DMT2 + 0,1 ml/kg BB EMPSPM (0,1); kelompok DMT2 + 0,2 ml/kg BB EMPSPM (0,2), kelompok kontrol DMT2 (DMT2), dan kelompok sehat (KS). Induksi DMT2 dengan menggunakan streptozotosin nikotinamid (STZ-NA). Terapi mulai dilakukan pada hari ke 7 setelah kondisi DM tercapai, diberikan 4 kali dengan selang waktu 7 hari secara intraperitoneal. Data kadar glukosa darah dan MDA dianalisi secara statistik. Hasil penelitian menunjukkan bahwa induksi DMT2 dengan STZ-NA dapat menaikkan kadar glukosa dan MDA dalam darah (P<0,05). Terapi 0,05; 0,1; dan 0,2 ml/kg BB EMPSPM menunjukkan dapat menurunkan kadar glukosa darah, kadar MDA plasma dan ginjal (P<0,05). Kadar 0,2 ml/kg BB EMPSPM menunjukkan aktifitas lebih baik dalam menurunkan kadar glukosa darah dan MDA. Berdasarkan penelitian ini, EMPSPM dapat menurunkan kadar glukosa darah serta kadar MDA dalam darah dan ginjal tikus DMT2.
ISOLASI AND IDENTIFICATION OF BACTERIA FROM THE URINE FLUID ORGANIC FERTILIZER (POC)ETTAWA CROSSBRED (PE) IN THE SLEMAN REGENCY Suwito, Widodo; Wahyuni, A.E.T.H.; Nugroho, Widagdo Sri; Sumiarto, Bambang; Bekti, Utomo Bimo
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.569 KB) | DOI: 10.22146/jsv.3781

Abstract

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.  
Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Nugroho, Widagdo Sri; Sudarwanto, M.; Lukman, D.W; Setiyaningsih, E.; Usleber, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 4 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces
PROTEKTIVITAS SAPI DI KABUPATEN KUPANG TERHADAP PENYAKIT NGOROK (SEPTICAEMIA EPIZOOTICA) (PROTECTIVITY AGAINST SEPTICAEMIA EPIZOOTICA OF COWS IN KUPANG DISTRICT) Berek, Hilda Susiyanti Debora; Nugroho, Widagdo Sri; Wahyuni, Agnesia Endang Tri Hastuti
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Controlling SepticemiaEpizooticae (SE) through vaccination program has been undertaken in KupangDistrict. However, numbers of fatal cases is still being reported. A cross sectional study have been undertakenin order to determine the prevalence and factors affecting herd immunity at farm level in Kupang District.A total of 434 cow?s sera from 87 farmers were collected and further analyzed using Enzyme-linkedImmunosorbent Assay (ELISA). An antibody titer of e?200 ELISA Unit was used as the indicator ofprotective immunity. The prevalence of herd immunity was 73.7% and vaccination coverage was 90.8%.Based on unweighted logistic regression analysis it was found that factors affecting the animals protectiveimmunity were: animal >2 years of age (r = +1.45601; OR = 4.3); housing system ( r = +1.03958; OR = 2.8);reservoir animals (r = +0.090147; OR = 2.5), the sex of the animal (female) (r = + 0.080138; OR =2.2); oncefrequency of vaccination (r = +0.61015; OR = 1.8); and 6-12 months post vaccination period (r = + 0.58968;OR = 1.8). Based on linier regression analysis, the prevalence of herd immunity was increased during rainyseason, whereas it was decreased when cows werebought from animal market or from other district.
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57
PENGEMBANGAN MEDIA PADAT UNTUK MENUMBUHKAN MYCOBACTERIUM BOVIS (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Daulay, Mazdani Ulfah; Sudarwanto, Mirnawati; Nugroho, Widagdo Sri; Sudarnika, Etih
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.
Pengetahuan, sikap, dan faktor risiko lingkungan pada rumah tangga dengan riwayat leptospirosis di kota Yogyakarta dan kabupaten Bantul Rakebsa, Defryana; Indriani, Citra; Nugroho, Widagdo Sri
Berita Kedokteran Masyarakat (BKM) Vol 34, No 4 (2018)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.309 KB) | DOI: 10.22146/bkm.28562

Abstract

Tujuan: Penelitian ini untuk mengetahui hubungan pengetahuan, sikap, dan faktor lingkungan dengan kejadian leptospirosis.Metode: Rancangan penelitian ini adalah observasional dengan studi case control dan studi lingkungan. Penelitian ini dilakukan pada bulan November 2016 di Kota Yogyakarta dan Kabupaten Bantul pada 242 responden. Kasus ialah penderita leptospirosis yang tercatat di dinas kesehatan Kota Yogyakarta dan Kabupaten Bantul pada rentang waktu bulan Juni 2014 sampai bulan Juni 2016. Kontrol ialah rumah tangga yang berada di lingkungan yang sama dengan kasus dan tidak memiliki riwayat leptospirosis dengan perbandingan kasus dan kontrol adalah 1:1. Penangkapan tikus dilakukan di dalam rumah dan di sawah.Hasil: Pengetahuan (OR: 1,95% CI: 0,58-1,71:p-value 1)  dan sikap (OR:0,8, 95% CI: 0,47-1,40:p-value 0,44) tidak memiliki hubungan signifikan dengan kejadian leptospirosis. Keberadaan tikus didalam rumah tidak memiliki hubungan signifikan dengan (OR : 0,7, 95% CI: 0,15-3,78:p-value 0,73). Hasil perhitungan multivariat menemukan bahwa variabel jarak rumah dengan saluran terbuka( OR:2.96, 95% CI:1,22-7,14) dan keberadaan sampah di dalam rumah (OR: 2.03, 95%CI:1,14-3,62) memiliki hubungan yang signifikan dengan kejadian leptospirosis.Simpulan: Tidak ada hubungan yang bermakna secara statistik antara pengetahuan dan sikap dengan kejadian leptospirosis. Faktor lingkungan seperti jarak rumah dengan saluran terbuka dan keberadaan sampah di dalam rumah memiliki hubungan yang bermakna secara statistik dengan kejadian leptospirosis.  Dua dari 4 serum tikus yang diperiksa dengan metode MAT positif mengandung Leptospira dengan strain Benjamin serovar Benjamini.
Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Nugroho, Widagdo Sri; Sudarwanto, M.; Lukman, D.W; Setiyaningsih, E.; Usleber, E.
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces