Articles

Found 28 Documents
Search

ISOLASI DAN UJI PENANGKAPAN RADIKAL BEBAS DPPH OLEH ISOLAT-1, FRAKSI ETIL ASETAT, DAN EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia Jack) Nurani, Laela Hayu
PHARMACIANA Vol 3, No 1: Mei 2013
Publisher : PHARMACIANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.03 KB)

Abstract

Radikal bebas dihasilkan pada waktu menjalankan proses-proses metabolit atau melawan infeksi maupun sewaktu tubuh mencerna makanan. Radikal bebas yang tidakstabil dapat dinetralisi dengan antioksidan. Akar pasak bumi (Eurycoma longifolia Jack.) diketahui mengandung senyawa flavonoid, yang diketahui mempunyai aktivitas sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kemampuan aktivitas penangkapan radikal bebas fraksi air dan fraksi etil asetat ekstrak etanol akar pasak bumi dengan menggunakan metode DPPH (1,1-difenil-2-pikrilhidrazil). Akar pasak bumi diekstraksi dengan etanol 70% menggunakan maserasi. Ekstrak etanol dilarutkan dalam etil asetat, fraksinasi dengan etil asetat sehingga diperoleh fraksi etil asetat. Konsentrasi ekstrak etanol dan fraksi etil asetat yang digunakan adalah 2; 4; 8; 16 ug/mL dan isolat-1 yaitu: 0,8; 1,6; 3,2; dan 6,4 ug/mL. Hasil penelitian menunjukkan bahwa semua perlakuan mempunyai aktivitas sebagai penangkap radikal bebas. Hasil analisis statistika dengan metode Kruskal Wallis pada taraf kepercayaan 95% yang dilanjutkan dengan uji Mann whitney menunjukkan adanya perbedaan aktivitas penangkapan radikal bebas yang signifikan antara masing-masing kelompok perlakuan. Kesimpulan dari penelitian ini adalah diperolehnya harga ES50 ektrak etanol (15,64 ug/mL) lebih besar daripada fraksi etil asetat (13,948 ug/mL) lebih besar daripada isolat 1 (3,961).
EFEKTIVITAS PERLAKUAN KOMBINATIF PLASMA MEDIS DAN EKSTRAK DAUN SIRIH UNTUK MEMPERCEPAT PENYEMBUHAN LUKA FASE PROLIFERASI PADA MODEL MENCIT DIABETIK Wahyuningtyas, Eka Sakti; Nasruddin, Nasruddin; Rahayu, Heni Setyowati Esti; Lutfiyati, Heni; Sikumbang, Isabella Meliawati; Nurani, Laela Hayu; Rohmani, Afiana; Salsabila, Nia; Putri, Gela Setya Ayu
Jurnal Ilmiah Kesehatan Keperawatan Vol 15, No 2 (2019): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM STIKES MUHAMMADIYAH GOMBONG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jikk.v15i2.378

Abstract

The continued increase in the number of people with Diabetes Mellitus (DM) in Indonesia is a serious problem. One of the big problems for people with Diabetes Mellitus (DM) is the emergence of complications of diabetic wounds. To date the strategy for treatment of diabetic wounds has been limited to the use of wound dressing, cell therapy and oxygen therapy. The problem is that the strategy is not fully successful. Thus, it is very important to look for new strategies to improve the quality of diabetic wound healing, such as by applying a combination of plasma medicine and local natural product, like the extraction of Daun sirih (Piper betle) leaves. Plasma medicine is a relatively new and multidisciplinary study involving plasma science, biomedical, pharmaceutical and other health sciences aimed at applying plasma to therapeutic health. Plasma is the fourth phase of matter, after the solid, liquid and gas phase. The medical aspects of plasma are related to the ability of plasma to produce biological molecules Reactive Oxygen and Nitrogen Species (RONS). If RONS is controlled in the right dosage it can be efficacious for health therapy. This study intends to examine the effects of combinative treatment of plasma medicine and Piper betel leaf extract for proliferation phase of wound healing in diabetic small animal model. This study used male Balb c mice with acute wounds which were divided into 5 groups, namely groups of untreated normal mice (ND-TP), groups of untreated diabetic mice (D-TP), groups of diabetic mice wounds with Piper betel leaf extract (DS ), the wound group of diabetic mice with plasma medicine (DP) and the wound group of diabetic mice with plasma medicine and Piper betel leaf (DPS). The plasma medicine was treated on wound with condition non-contact style (the plasma jet did not touch the wound) with a distance of plasma jet reactor nozzle to the surface of wound about 20 mm, for 2 minutes, every day. Macroscopic observation of wounds is carried out every day from day 0 to 7. On day 7 it was seen that the size of the wound area for D-P-S was smaller than the other groups. The results of this study indicated that Piper betel leaf extract can potentially be used to optimize the performance of plasma medicine in accelerating diabetic wound healing during the proliferation phase. Further investigation, however, is important to be conducted to study the effect for all phases of wound healing and its mechanism histo-pathologically.
UJI AKTIVITAS ANTIBAKTERI INFUSA DAUN SIRSAK (Annona muricata L.) SECARA in Vitro TERHADAP Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 35218 SERTA PROFIL KROMATOGRAFI LAPIS TIPISNYA Yeni, Yeni Dianita; Djannah, Sitti Nur; Nurani, Laela Hayu
Jurnal Kesehatan Masyarakat (Journal of Public Health) Vol 4, No 3 (2010): Jurnal Kes Mas FKM UAD September 2010
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.302 KB)

Abstract

Background: A research has been done to test the In vitro antibacterial activities of sirsak leaf(Annona muricata L.) infuse toward Staphylococcus aureus ATCC 25923 and Escherichia coliATCC 35218 to analyze its thin layer chromatography profile. This research done to observe theantibacterial activity from sirsak leaf infuse.Method: A test on the antibacterial activity was done by using liquid dilution method. Theconcentration infuse in sterile destilate water using to test the antibacterial activity toward S.aureus were 100%, 95%, 90%, 85%, 80%, and 75% w/v, while toward E. coli were 100%, 90%,80%, 70%, 60%, and 50% w/v. To detect Chemical contains of the sirsak leaf infuse wereidentified using Screening method of Phytochemistry and Thin Layer Chromatography (TLC).Result: The result showed that the Minimum Bacterial Concentration (MBC) of sirsak leaf infuseS. aureus were 85% w/v, while E. coli could not be show antibacterial activity until 100% w/vconcentration. The Minimum Inhibition Concentration (MIC) could not be identified by the liquiddilution because the mixture were turbid and the colour is dark brown. Screening method ofPhytochemistry use tube-test and Thin Layer Chromatography showed that the infuse containflavonoid, poliphenol, and alkaloid.Conclusion: Infuse of sirsak leaf (Annona muricata L.) has antibacterial activity againstStaphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 profil infuse. Thin LayerChromatography showed that the infuse contain flavonoid, poliphenol, and alkaloid.Keyword: Antibacterial, sirsak leaf, chromatography
PENGARUH PEMBERIAN EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia Jack) TERHADAP EKSPRESI PROTEIN p53 PADA KANKER PAYUDARA TIKUS BETINA SPRAGUE DAWLEY (SD) YANG DIINDUKSI 7,12-Dimetilbenz[α]anthrasen (DMBA) Nurani, Laela Hayu
Pharmacon Vol 11, No 1 (2010)
Publisher : Pharmacon

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Akar pasak bumi mengandung senyawa kuasinoid diduga mempunyai efek penghambatan karsinogenesis, antiulcer, dan antimalaria. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian eksktrak etanol akar pasak bumi terhadap ekspresi p53 pada kanker payudara tikus betina yang diinduksi DMBA.Tikus dibagi menjadi 6 kelompok. Kelompok I, II dan III diberi ekstrak etanol akar pasak bumi dosis berturut-turut 100; 200 dan 400 mg/kg BB. Kelompok IV diberi larutan DMBA 20 mg/kg BB. Kelompok V diberi corn oil. Kelompok VI sebagai baseline. Tikus yang mengalami kanker, dihitung jumlah nodul tumornya dan pada minggu ke-23 semua tikus dikorbankan dan diambil jaringannya untuk pengamatan secara mikroskopik menggunakan metode H&E dan imunohistokimia. Hasil penelitian menunjukkan bahwa rerata ekpresi p53 mutant pada pemberian ekstrak berturut-turut 100, 200 dan 400 mg/kg BB adalah 6,375 ± 4,07; 11,25 ± 16,53; dan 0,875 ± 1,75; kelompok  IV (DMBA) sebesar 13,125 ± 14,4; kelompok V (corn oil) adalah 1,375 ± 1,55; kelompok VI (baseline) adalah 0,375 ± 0,75. Hasil penelitian disimpulkan bahwa ekstrak etanol akar pasak bumi (Eurycoma longifolia Jack) dosis 400 mg/kg BB yang diberikan sebelum dan selama induksi DMBA mampu menurunkan ekspresi protein p53 mutant (proapotosis) dan mampu menghambat pertumbuhan kanker payudara tikus betina Sprague Dawley yang diinduksi 7,12-dimetilbenz(α)antrasen (DMBA).Kata kunci:  akar pasak bumi (Eurycoma longifolia Jack), kemopreventif, imunohistokimia, ekspresi p53
UJI SITOTOKSISITAS, ANTIPROLIFERATIF, DAN PENGARUHNYA TERHADAP EKSPRESI P53 DAN BCl2 DARI FRAKSI ETANOL INFUSA DAUN TEH (Camellia sinensis (L.) O.K.) TERHADAP SEL HeLa Nurani, Laela Hayu
Majalah Obat Tradisional Vol 16, No 1 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.058 KB) | DOI: 10.14499/mot-TradMedJ16iss1pp%p

Abstract

Daun teh (Camellia sinensis  (L.) O.K.) merupakan salah satu bahan alam yang digunakan masyarakat untuk pengobatan tradisional sebagai antikanker. Penelitian ini bertujuan untuk mengetahui efek sitotoksisitas, antiproliferatif, dan mekanisme terhadap p53 dan bcl-2  dari fraksi etanol infusa daun teh (Camellia sinensis  (L.) O.K.). Daun teh yang diperoleh, diekstraksi dengan cara infundasi dan difraksinasi dengan pelarut etanol. Uji sitotoksisitas dilakukan dengan menginkubasi sel HeLa dengan kepadatan 2.104 dengan perlakuan ekstrak kadar 250 µg/mL; 125  µg/mL; 62,5; 31,25; 15,63; dan 7,81 µg/mL selama 24 jam. Uji antiproliferatif dilakukan dengan menghitung  jumlah sel hidup pada perlakuan sampel kadar 31,25; 15,63; 7,81; dan 3,91 µg/mL setelah diinkubasi pada jam ke-24, 48 dan 72. Uji imunositokimia dilakukan pada konsentrasi sebesar 24,45 µg/mL  dengan antibodi p53 dan bcl-2.  Ekspresi p53 dan bcl-2   dibandingkan dengan kontrol. Hasil penelitian menunjukkan bahwa sampel fraksi etanol dari infusa daun teh (Camellia sinensis  (L.) O.K.) bersifat sitotoksik terhadap sel HeLa dengan harga LC­­50 sebesar 24, 45 µg/mL. Hasil uji doubling time diperoleh doubling time kontrol pelarut 74,11 µg/mL dan kontrol sel 78,22 µg/mL. Sedangkan pada perlakuan kadar 31,25; 15,63; 7,81; dan 3,91 µg/mL diperoleh harga slope yang negatif sehingga tidak diperoleh harga doubling time.  Ekstrak tersebut dapat memacu ekspresi p53 dan menghambat ekspresi bcl-2 dibandingkan dengan kontrol.
UJI AKTIVITAS PENGHAMBATAN POLIMERISASI HEME (1)-N-(2-NITROBENZIL)-1,10- FENANTROLINIUM IODIDA DAN (1)-N-(4-NITROBENZIL)-1,10- FENANTROLINIUM IODIDA SECARA IN VITRO Nurani, Laela Hayu; Utami, Dwi; Widyaningsih, Wahyu; Narwanti, Iin; Nurwening, Eti; Jumina, Jumina
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v4i2.1575

Abstract

The inhibitory activity of heme polymerization of (1)-N-(2-nitrobenzyl)-1,10- phenantrolinium iodide and (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide have been done. This study aims to analyse the (1)-N-(2-nitrobenzyl)-1,10-phenantrolinium iodide and (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide as inhibitory of polimerization heme. Analysis of heme inhibtory polimerization activity used the experimental in vitro method. The activity showed by IC50 (the capable concentration of extract to inhibiting polymerization heme by 50% ). The IC50 value acquired by probit analysis. Assess IC50 of (1)-N-(2-nitrobenzyl)- 1,10-phenantrolinium iodide not to be identified, (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide and chloroquine by successively are 0,571±0,071; 25,498±1,876 mg/mL. The result showed the (1)-N-(4-nitrobenzyl)-1,10-phenantrolinium iodide had the highest value of the heme polymerization inhibitory activity than chloroquin, (1)-N-(2-nitrobenzyl)-1,10- phenantrolinium iodide hadn?t the heme polymerization inhibitory activity.
UJI SITOTOKSISITAS DAN ANTIPROLIFERATIF EKSTRAK ETANOL DAUN LEUNCA (SOLANUM NIGRUM,L) TERHADAP SEL RAJI Dona, Rahma; Sulistyani, Nanik; Nurani, Laela Hayu
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v6i2.3506

Abstract

Leunca (Solanum nigrum,L.) is one of the medicinal plant which used society for traditional medication as antipyretic, hypotensive and anticancer. The aim of  this research was to know an anticancer activity of ethanol extract of leunca leaves, emphasized on an ability to inhibit the growth Raji cell. Ethanol extract took from leunca leaf powder that epitomized using ethanol solvent by Soxhlet instrument. Citotoxicity test was done by incubating Raji cell at a density of 2x104 with treatment using ethanol extract from leunca (Solanum nigrum L.) leaf in several concentration 500; 250; 125; 62,5; 31,25; 15,62; 7,81 and 3,90 µg/ml. A test was done by MTT method and the percent of cell mortality was calculated. The LC50 values were calculated using probit analysis. The research continued with antiproliferation test on treatment sample concentration 50; 25 µg/ml with cell control for 24, 48, and 72 hours.The result of research indicate that ethanol extract of leunca leaves had cytotoxic effect towards Raji cell with LC50 values 59,22 µg/ml. The result of  antiproliferation test showed that there were the growth of inhibitation on treatment sample with doubling time values 69,56 hour at concentration 50 µg/ml; 60,00 hour at concentration 25 µg/ml, and doubling time cell control is 44,98 hour.
Efek flavonoid daun srikaya (Annona squamosa (L)) terhadap pemacuan ekspresi P53 sel HeLa Nurani, Laela Hayu; Pramono, Suwidjiyo; ., Sudjadi
INDONESIAN JOURNAL OF PHARMACY Vol 22 No 4, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp273-278

Abstract

Flavonoids  are  secondary  metabolites  with  many  activities  such  as  an anticancer  and  an  antioxidant. Annona  squamosa L.  leave  are  reported  contain flavonoids. Annona squamosa flavonoids were isolated from ether fraction and separated by paper chromatography with acetic acid 15% as mobile phase. Four flavonoids   with  Rf  value  of  0.11,  0.23,  0.46  and  0.78  were  detected  as  5-hydroxy-7-R-flavonol  (1),  5,3’,4’-trihydroxy-7-R-flavonol  (2),  5-hydroxy-7-Rflavanone  (3)  and  7-R-flavanone  (4),  respectively.  The  cytotoxicity  of  each flavonoid  on  Hella  cell  line  was  carried   out   using  direct  counting  method  and the p53 expression was done with immunocytochemistry. The result showed that LC50  of  flavonoid 1, 2, 3 and  4 was  12.63±3.4,  18.47±3.4,  75.74±6.2  and 204.4±14.0  µg/mL.   Twelve   µg/mL  of  5-hydroxy-7-R-flavonol  (1)   increased p53 expression of eleven fold than control.Keywords: Annona squamosa, HeLa, flavonoid, p53 
EFEK KO-KEMOTERAPI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP PROLIFERASI DAN EKPRESI BAX JARINGAN PAYUDARA TIKUS SD Zakinah, Tria; Nurani, Laela Hayu; Widyarini, Sitarina
Journal of Pharmaceutical Sciences and Community Vol 14, No 1 (2017)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.051 KB) | DOI: 10.24071/jpsc.141559

Abstract

The use of co-chemotherapy agent was needed since there were some toxicities on the normal tissues caused by the use of doxorubicin. The root of Eurycoma longifolia Jack, a part of plant, has a potential activity as co-chemotherapy. This study aimed to determine the effect of co-chemotherapy of ethyl acetate fraction of E. longifolia Jack roots and doxorubicin against cell proliferation activity by AgNOR method and determine expression of Bax protein of breast tissue in rats induced by DMBA. The rats were divided into five groups: I (baseline), II (DMBA 20 mg/kgBW), III (DMBA, doxorubicin 1.12 mg/kg), IV (DMBA, fraction of 100 mg/kg), and V (DMBA, doxorubicin, fractions). All the rats were sacrificed at week 16. Their breast tissue was evacuated. Cell proliferation and expression was observed using AgNOR method and immunohistochemistry, respectively. Results showed the percentage of p-AgNOR obtained by the healthy group, DMBA group, DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were 0±0%, 14.67±2.11%, 1.83±1.21%, 6.83±2.03%, and 4.08±0.95%, respectively. The percentage of Bax expression obtained by the healthy group, DMBA group, DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were 68.82±1.52%, 26.86±3.25%, 44.49±2.06%, 80.92±3.27%, and 78.70±4.87%, respectively. Based on the results, it was concluded that co-chemotherapy agent of ethyl acetate fraction of E. longifolia Jack roots and Doxorubicin could inhibit proliferation and trigger apoptosis through Bax expression in breast tissue of rats induced by DMBA. 
PENGARUH PEMBERIAN SEDIAAN NANOPARTIKEL KITOSAN EKSTRAK ETANOL ROSELA (Hibiscus sabdariffa L.) PADA TIKUS HIPERKOLESTEROL TERHADAP PROFIL LIPID. Safitri, Meta; Nurkhasanah, Nurkhasanah; Nurani, Laela Hayu
Kartika Jurnal Ilmiah Farmasi Vol 2, No 1 (2014)
Publisher : Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/kjif.v2i1.9

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian Sediaan Nanopartikel Kitosan Ekstrak Etanol Rosela (SNKEER) terhadap profil lipid tikus galur Sprague Dawley yang diinduksi hiperkolesterol. Pembuatan SNKEER dilakukan berdasarkan hasil optimasi dengan perbandingan 2:1:1/10 (ekstrak etanol rosela (EER) : kitosan : TPP), selanjutnya dilakukan uji in vivo dengan menggunakan Tikus putih betina galur Sprague Dawley (SD) umur 6-8 minggu sejumlah 25 ekor dibagi menjadi 5 kelompok, yaitu kelompok I (K1) adalah kelompok kontrol diberi pakan standar; kelompok II (K2) diinduksi hiperkolesterol; serta kelompok III (3), IV (4), dan V (5) diinduksi hiperkolesterol dan SNKEER masing-masing dengan  dosis 25, 50, dan 100 mg/kgBB, selama 30 hari. Pada hari ke 31 semua hewan uji diambil darahnya melalui vena orbitalis mata untuk dilakukan pengukuran profil lipid (Kolesterol Total, kadar LDL, HDL, Trigliserida). Analisis yang dilakukan terhadap penelitian ini adalah One Way Analisis Of Variance (ANOVA) dengan uji lanjutan Tukey 5 %.Hasil penelitian rata-rata pengukuran profil lipid adalah sebagai berikut : pada pengukuran Kolesterol K1, K2, K3, K4,K5 berturut-turut yaitu 106,76; 233,45; 197,63; 158,61; 133,94 mg/dl, Trigliserida : 73,33; 125,51; 107,97; 90,43; 80,87 mg/dl, HDL: 44,41; 14,45; 18,19; 23,68; 30,90mg/dl , LDL: 51,58; 72,24; 63,16; 54,61; 51,84 mg/dl.  Hasil dari analisis data menunjukkan bahwa kelompok SNKEER dosis (25, 50, 100 mg/kgBB) dapat berpengaruh signifikan terhadap perbaikan  profil lipid terutama dalam menurunkan kadar kolesterol total, trigliserida, dan kadar LDL serta dapat meningkatkan kadar HDL secara signifikan bila dibandingkan dengan kelompok hiperkolesterol (K2). Adapun kelompok perlakuan yang memberikan hasil perbaikan profil lipid  terbaik adalah kelompok SNKEER dosis 100 mg/kgBB bila dibandingkan dengan semua kelompok perlakuan (P<0,05).