Wisnu Nurcahyo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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DAYA LARVASIDA EKSTRAK DAUN MUDA KEDONDONG HUTAN TERHADAP HAEMONCHUS CONTORTUS SECARA IN-VITRO (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) Wirawan, I Gusti Komang Oka; Nurcahyo, Wisnu; Prastowo, Joko; Kurniasih, Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.02 KB) | DOI: 10.19087/jveteriner.2017.18.2.283

Abstract

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).  
STRONGYLOIDES SPP DISTRIBUTION ON ORANGUTANS IN TANJUNG PUTTING NATIONAL PARK, CARE CENTER IN PANGKALANBUN, AND SEBANGAU NATIONAL PARK Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.949 KB)

Abstract

Strongyloides spp is a parasitic nematode in livestock, primate and human which is  considered asa danger zoonotic disease. Therefore, study about parasite distribution is very important in order to find outgenetic diversity among orangutan in quarantine, zoo and nature, as an effort to explore infection patternand life cycle of Strongyloides spp on orangutan. Amount of 326 orangutan feces were taken from threedifferent habitat of orangutan in Central Borneo, Tanjung Puting National Park, Orangutan Care Centerand Sebangau National Park. Samples which were collected from Tanjung Puting, Care Center and Sebangauwere 75, 80 and 171 respectively. Those samples were transported to the Parasitology laboratory in Facultyof Veterinary Medicine, Gadjah Mada University, Yogyakarta for examination and detection.  Prevalence ofstrongyloides in Tanjung Putting, Sebangau and Orangutan Care Center were 24%, 14,6% and 13,3%respectively. Among positive samples of Strongyloides, 62,5% were from male orangutans, while 37,5% werefrom female orangutans. Strongyloides in pre adult and baby orangutan were 91,6% and 4,2% respectively.Meanwhile, Strongyloides in adult orangutan were very rare. Orangutan habitat in Sebangau National Parkis an ideal habitat for orangutan, supported by the watery condition of peat land, so that Strongyloides re-infection become difficult. Some factors may have important role in Strongyloidoses, such as behavior,physical condition, nutrition, age, body weight, sex, immunity and social status of orangutan.
FAKTOR RISIKO INFEKSI TOXOPLASMA GONDII PADA KUCING DOMESTIK YANG DIPELIHARA DI YOGYAKARTA Hanafiah, Muhammad; Nurcahyo, Wisnu; Prastowo, Joko; Hartati, Sri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.963 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2792

Abstract

Tujuan penelitian adalah menentukan kemungkinan faktor-faktor risiko terhadap kemunculan toksoplasmosis. Sebanyak 132 sampel serum darah diambil pada kucing lokal di Yogyakarta. Data-data epidemiologis seperti asal usul kucing, ras kucing, jenis kelamin, umur, lokasi sistem pemeliharaan, jenis pakan, dan frekuensi diare diberi kode untuk mempermudah analisis, kemudian dimasukkan, disimpan, dan dianalisis dengan program Statistix Versi 7 (Analytical Sofware inc). Analisis data dilakukan secara bivariat (Chi-square (?2), dan kekuatan asosiasi (OR), dan multivariat (regresi logistik). Hasil penelitian menunjukkan bahwa faktor-faktor yang berpengaruh terhadap adanya toksoplasmosis pada kucing berdasarkan analisis bivariat adalah pembersihan kotak pasir 1 kali sehari dan mandi 2-3 kali seminggu sedangkan faktor-faktor yang memiliki peluang meningkatkan seropositif toksoplasmosis berdasarkan analisis multivariat adalah pemeliharaan kucing yang bebas di dalam rumah, dimandikan lebih dari 1 kali seminggu, dan dimandikan lebih besar dari 1 bulan sekali.
PENGARUH INFUSA BIJI BUAH PINANG (ARECA CATECHU) TERHADAP TINGKAT KEMATIAN DAN MORFOMETRI CACING DEWASA ASCARIDIA GALLI SECARA IN VITRO Mubarokah, Wida Wahidah; Kurniasih, Kurniasih; Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.658 KB) | DOI: 10.22146/jsv.43751

Abstract

The study evaluates in vitro effects of Areca catechu crude aqueous extract  as anthelmintics on Ascaridia galli adult mortality rate and morphometry. Naturally infected chickens were collected from slaughtered chickens at local slaughterhouses in Yogyakarta. Chicken?s intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. Ascaridia galli were collected from intestines (including duodenum, jejunum, and ileum) and put into a petri dish containing 0.62% saline water. There were 60 A. galli in each concentration of Areca catechu crude aqueous extract. Investigation on mortality rate of adult worms at various concentration and observation as well as differences in A. galli morphometry and mortality rate were analyzed using analysis Anova. The figures of the parasites or the parts of the parasites were captured using camera Lucida and then they were measured using both micrometer and curvimeter. The morphology of the parasites was identified to find out their morphometric characteristics.The results of morphometric observation of the A. galli showed that there was a significant difference in the body width and the vulva length of the females and in the body width, the esophageal length and the esophageal width of the males.This research disclosed that Areca catechu crude aqueous extract significantly affected the mortality rate of adult A.galli at various concentration. This research observed that the best concentration to kill the adult worms was 25% Areca catechu crude aqueous extract.
MONITORING OF PHYSIOLOGICAL AND PARASITES STATUS OF BAWEAN DEER (AXIS KUHLII) IN ITS HABITAT AS A BASELINE FOR WILDLIFE CONSERVATION ENDEAVOR Nurcahyo, Wisnu; Anggraeni, Devita; Imron, M.A.
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.17882

Abstract

AbstractThe research on physiological and reproduction status of Bawean deer (Axis kuhlii) in its habitat has been conducted, to understand and to find out as a basic information on Bawean deer (Axis kuhlii) in its habitats a baseline data for wildlife conservation efforts. The deer is categorized as an endangered animal, therefore, more attention was given toward Bawean deer conservation. Habitat changes, loss of habitat, fragmentation and illegal hunting might caused the wild animals become more marginalized and the populations have been declined.Therefore, it is needed to have research as an effort to save Bawean deer in its natural habitat. This research activity covered monitoring on physiological and reproduction status, and also the examination of deer's feces samples was parasite coprological data that serve as a basic consideration data in conservation and determination of Bawean deers health in the nature and/or conservation. The research was done in Bawean Island, Gresik and East Java, by taking faces of Bawean deers in the nature and conservation. Data gathering of physiological andreproduction status were done by examining deer's condition, either directly in conservation or in its habitat, and also by interviewing people around the forest. The data on physiological status, behavior monitoring, reproduction status, faces samples, blood, food analysis, and interview result were analyzed descriptively. Some worm eggs were found during feces examination, those were Strongyl, Strongyloides sp, Trichuris sp, Fasciola gigantica and Oocysta coccidia. The result showed that the same parasites were found in the cattle and goat raised by people around in the vicinity of the forest, meaning, there was an interaction between wildlife andlivestock. According to the result, it is needed to monitor the physiological status of Bawean deers routinely since Bawean deers is classified as endangered species.
DAYA OVICIDAL EKSTRAK KULIT BUAH MUDA (CALOTROPIS PROCERA) TERHADAP HAEMONCHUS CONTORTUS SECARA IN VITRO Wirawan, I Gusti Komang Oka; Nurcahyo, Wisnu; Prastowo, Joko; ., Kurniasih
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.23 KB) | DOI: 10.22146/jsv.17891

Abstract

Penelitian ini bertujuan untuk mengetahui konsentrasi efektif ekstrak kulit buah muda Calotropis procera (C. procera) dalam menghambat perkembangan telur cacing Haemonchus contortus secara in-vitro. dari 0,2g/ml sediaan larutan ekstrak stok, albendazole konsentrasi 0,055%. Data hasil penelitian dianalisis secara deskriptif. Konsentrasi efektif ekstrak kulit buah muda C. procera (EKBMCP) dalam menghambat perkembangan daya tetas telur cacing Haemonchus contortus secarain-vitro adalah perlakuan EKBMCP konsentrasi 4,5% dengan daya hambat 88% sedangkan  perlakuan EKBMCP konsentrasi 2,5% dan 3,5% daya hambatnya secara berturut-turut adalah 70,5% dan 81%.
STUDY OF TOXOPLASMOSIS INFECTION IN HUMAN AND RELATED TO ANIMAL IN BANDA ACEH Hanafiah, Muhammad; Kamaruddin, Mufti; Nurcahyo, Wisnu; Winaruddin, Winaruddin
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.638 KB) | DOI: 10.21157/j.ked.hewan.v4i2.9813

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The research has been done to know prevalence of toxoplasmosis in livestock, to search the source of infection that have potency to cause toxoplasmosis in human and to make a map of toxoplasmosis infection area in Banda Aceh. The method used in this research was serologic test, Card Aglutination Test (CATT). Data of fertile women in this study are collected through questioner. The result of this research showed that the toxoplasmosis prevalency number of society in Banda Aceh was 3.15%, while at livestock were varied respectively: goats 40%, chickens 25%, cattles 23%, duck 20%, cats 16%, buffalos 15%, and sheeps 10%. The potencial sources of to coures toxoplasmosis in human are: goat, chicken, cattle, duck, cat, buffalo, and sheep. The area found toxoplasmosis in humans and animal were: Baiturrahman, Kuta Raja, Ulee Kareng, Kuta Alam, and Syiah Kuala. Model toxoplasmosis infection patterns in human related to livestock and animal in Banda Acheh are : Y ( POSTOKSO) - 1,55688 + 2,65280 AYA + 1,17709 JDG + 4,28482 KUC - 3,74609 MEM
DAYA ANTHELMINTIK INFUSA BIJI BUAH PINANG (ARECA CATECHU) TERHADAP CACING ASCARIDIA GALLI SECARA IN VITRO Mubarokah, Wida Wahidah; Nurcahyo, Wisnu; Kurniasih, Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7800.591 KB) | DOI: 10.22146/jsv.40337

Abstract

A. galli infection caused high economic losses and decreased the amount and quality of egg production. The existence of anthelmintic resistance lead to the development of research on alternative treatments for A. galli. This aim of the research was to know LC50 of Areca catechu on infestation of A. galli. In this research A. galli was taken from a chicken slaughterhouse in Terban, Yogyakarta, and was divided into 8 groups for in vitro research. Group I was treated with an 10% infusion of A. catechu; group II was treated with 12.5% infusion of A. catechu; group III was treated with a 15% infusion of A. catechu; group IV was treated with a 17.5% infusion of A. catechu; group V was treated with 20% infusion of A. catechu; group VI was treated with a 22.5% infusion of A. catechu; group VII was treated with 25% A. catechu infusion and group VIII as negative control (0.9% NaCl). A. galli mortality is recorded every one hour until worm mortality is 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test the infusion of Areca catechu against Ascaridia galli can cause death in worms with an LC 50 calculation of 21. 18%.
IN VITRO DEVELOPMENT OF ASCARIDIA GALLI EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Mubarokah, Wida Wahidah; Kurniasih, Kurniasih; Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.993 KB) | DOI: 10.21157/j.ked.hewan.v13i1.12978

Abstract

The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
IDENTIFIKASI TOKSOPLASMOSIS PADA FESES KUCING SECARA MIKROSKOPIS DAN SEROLOGIS Nurcahyo, Wisnu; Prastowo, Joko; P, Priyowidodo
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.657 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2639

Abstract

Penelitian ini bertujuan mengetahui tingkat prevalensi Toxoplasma pada kucing dan menyusun basis epidemiologis pada kucing yang sangat penting dalam peranannya sebagai hospes definitif toksoplasmosis. Sampel serum darah dan feses kucing digunakan dalam penelitian ini. Metode pemeriksaan menggunakan card agglutination Toxoplasma test (CATT) Pastorex? Toxo kit dan metode sentrifus. Data yang dip eroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa tingkat prevalensi Toxoplasma dengan pemeriksaan serum darah kucing sebesar 6,8% dan pemeriksaan feses sebesar 9,4%. Infeksi toksoplasmosis yang terjadi pada kucing secara umum dari pemeriksaan klinis tidak mempunyai gejala yang spesifik.