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STABILITAS LAJU FOTOSINTESIS DAN PRODUKTIVITAS RIMPANG GENOTIPE CURCUMA AERUGINOSA DAN CURCUMA ZANTHORRHIZA Djarot Sasongko Hami Seno; Mohamad Rafi; Maria Bintang; Popy Asri Kurniatin; Waras Nurcholis
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v48i1.30100

Abstract

Curcuma aeruginosa Roxb. rhizome is an essential herb material with significant antimicrobial, anticancer, and antioxidant activities. Thus, it is important to search for C. aeruginosa plant that has the rhizome yield trait with more excellent stability in changing environmental situations. This research aimed to evaluate the genotype x environment interaction using additive main effects and multiple interaction (AMMI) analysis for photosynthetic rate and rhizome yield of C. aeruginosa genotypes. Twenty clones of C. aeruginosa plus three control varieties of C. zanthorrhiza Roxb. were evaluated in three environments (Bogor, Cianjur, and Sukabumi) using a randomized complete block design with three replicates. The photosynthetic rate and rhizome yield showed significantly (p < 0.01) genotype x environment interaction. The AMMI2 explained 100% variability of the photosynthetic rate and rhizome yield parameters. G2, G10, G12, G13, G14, G16, G17, G19, and G20 were stable genotypes for rhizome yield based on AMMI analyses. Meanwhile, the stable genotype in photosynthetic rate recorded in G4, G5, G6, G8, G11, G13, and G14. Keywords: AMMI, genotypes, multilocation trials, pink and blue ginger, varieties
EVALUASI KARAKTER AGRO-MORFOLOGI JENGGER AYAM (CELOSIA CRISTATA L.) PADA GENOTIPE MUTAN M3 Nella Angelina Simanjuntak; Syarifah Iis Aisyah; Nurcholis, Waras
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v48i1.29939

Abstract

Celosia cristata L., namely jengger ayam, is an ornamental plant with a unique structure. The flower of C. cristata is shaped grains with small size; thus, the hybridization for increasing the diversity in this plant is challenging. One alternate technique for improving diversity is mutation induction by gamma rays. This study aimed to evaluate the variability of agro-morphological traits in seventeen M3 mutant C. cristata genotypes at the Sabisa Farm garden. Agro-morphological characteristics were evaluated based on descriptors of the International Union for the Protection of New Varieties of Plants (UPOV) with modification. Analysis of variance was performed using SPPS 20.0, whereas a similarity analysis was performed using PBSTAT-CL 2.1. Significant variability among C. cristata genotypes (p < 0.05) was found for plant height and flower length. Three clusters based on agro-morphological traits were obtained from similarity analysis with the dissimilarity distance of 32%. The results indicated that the agro-morphology traits were able to differentiate the 17 genotypes of C. cristata studied, and therefore it can be used for further research development in plant breeding programs. Keywords: agro-morphological, diversity, mutation breeding, similarity analysis
ANTIOXIDANT, CYTOTOXIC ACTIVITIES AND TOTAL PHENOLIC CONTENT OF FOUR INDONESIAN MEDICINAL PLANTS Nurcholis, Waras; Priosoeryanto, Bambang Pontjo; Purwakusumah, Edy Djauhari; Katayama, Takeshi; Suzuki, Toshisada
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.4, Mei 2012
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.104 KB) | DOI: 10.15408/jkv.v2i4.267

Abstract

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 ?g/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 ?g/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.
ANALISIS KEMIRIPAN 20 AKSESI TEMU IRENG (CURCUMA AERUGINOSA ROXB.) BERDASARKAN WARNA RIMPANG, HASIL EKSTRAK, DAN KANDUNGAN FITOKIMIA Nurcholis, Waras; Khumaida, Nurul; Syukur, Muhamad; Bintang, dan Maria
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.174 KB) | DOI: 10.24831/jai.v44i3.12762

Abstract

ABSTRACTCurcuma aeruginosa Roxb., popularly known as ?temu ireng?, is considered as a potential source of medicinal plant for pharmacological activities. However, varieties of C. aeruginosa are still limited in Indonesia so it needs more accessions for improvement and development of new varieties. Rhizome colors, phytochemical contents and extract yield from 20 promising lines of C. aeruginosa were investigated by qualitative method for rhizome colors and phytochemical contents, and maceration method using 70% ethanol for yield extract. Similarity analysis was used for cluster analysis based on rhizome colors, phytochemical contents and yield extract. Blue was the color characterization of rhizome C. aeruginosa. The extract yield for 20 promising lines of C. aeruginosa varied from 7.92 to 19.71%, with KN and BH promising lines having the lowest and highest value, respectively. All promising lines of C. aeruginosa contain saponin and triterpenoid. Based on similarity analysis, all promising lines could be divided into 3 clusters. Cluster I consisted of 14 promising lines i.e. WG, SH, KA, GD, BH, KP, NW, PW, MB, PR, PT, KN, MD, and PK. Cluster II consisted of 4 promising lines i.e. LC, CB, KL, and GK. Cluster III consisted of 2 promising lines i.e. KD and SG. Keywords: promising lines, saponin, triterpenoid
ANALISIS KOMPARATIF KANDUNGAN METABOLIT PADA DAUN MUTAN TANAMAN TORBANGUN (PLECTRANTHUS AMBOINICUS (LOUR.) SPRENG.) Aisyah, Syarifah Iis; Rusmiyati, Henny; Sukma, Dewi; Damanik, Rizal; Nurcholis, Waras
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i1.109

Abstract

Torbangun (Plectranthus amboinicus) merupakan tanaman obat dari keluarga Lamiaceae. Pada masyarakat Batak, daun torbangun digunakan untuk meningkatkan produksi air susu. Keragaman kandungan fitokimia dapat ditingkatkan salah satunya dengan teknik iradiasi sinar gamma untuk program pemuliaan tanaman. Pada penelitian ini, metode metabolomik yang tidak tertarget digunakan untuk mengevaluasi profil senyawa pada bagian daun tanaman mutan dari iradiasi sinar gamma dan kontrol. Kandungan senyawa planlet mutan dan kontrol dianalisis dengan GC-MS. Data GC-MS dianalisis dengan kemometrik dengan menggunakan hierarchical cluster analysis (HCA). Keragaman kandungan kimia ditunjukkan oleh planlet mutan dan kontrol daun torbangun. Hasil analisa planlet kontrol torbangun menunjukkan terdapat lima senyawa utama diantaranya hydroxymethylfurfurole (35,15%), brevifolin (7,69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13,13%), stigmasterol (6,51%) dan ferruginol (8.63%). Hasil analisa mutan torbangun memiliki kandungan senyawa DDMP (19,57%), neophytadiene (8,47%), linolenic acid (9,10%), ferruginol (7,61%), stigmasterol (14,14%) dan gamma.-sitosterol (7,08%). HCA menunjukkan tiga komponen senyawa yang berbeda antara planlet kontrol dan mutan yaitu kenaikan kandungan senyawa DDMP dan stigmasterol, serta penurunan kandungan hydroxymethylfurfurole pada mutan torbangun.
Anti-inflammatory Activity of Temulawak Nanocurcuminoid Coated with Palmitic Acid in The Sprague Dawley Rat Novita, Rini; Ambarsari, Laksmi; Falah, Syamsul; Kurniatin, Popi Asri; Nurcholis, Waras; Darusman, Latifah K
Current Biochemistry Vol 2, No 2 (2015)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/73-85

Abstract

Temulawak or Curcuma xanthorrhiza Roxb, is usually used as traditional medicine (herbal medicine) that has antioxidant, anticancer, antihyperglycemic, anti-inflammatory, hepatoprotective and gastroprotective properties. The main components contained in temulawak responsible for its efficacy as a medicine are xantorhizol and curcuminoid. Curcuminoid has drawbacks, which are difficult to absorb and very quickly metabolized by the body, so that limit its bioavailability. The use of solid lipid nanoparticle carrier system (SLN) in form of palmitic acid, is known to improve the bioavailability of curcuminoid. This study aims to find the effective dose of nanocurcuminoid coated with palmitic acid that can be used as an anti-inflammatory agent. The methods used in this study, include the production of nanocurcuminoid with homogenization and ultrasonication methods, determination of particle size, polydispersity index, entrapment efficiency and anti-inflammatory activity test through rat feet edema. Nanocurcuminoid obtained in this study was 561.53 nm in size, with polydispersity index 0.309 and concentrations of curcuminoid absorbed and entrapment efficiency were 0.61±0.031 mg/mL, 58.93±3.021%, respectively. Anti-inflammatory activity of nanocurcuminoid through treated Sprague Dawley rats, showed that there were no significant difference compared with the positive control, curcuminoid extracts and empty nanoparticle. These results indicate that nanocurcuminoid with 175, 200 and 250 mg/kg.bw in doses, has greater anti-inflammatory activity (31.70%) compared to the other treatments.
Phytochemical Analysis, α-glucosidase Inhibition Activity in-vitro and Enzyme Kinetics of Ethyl Acetate and Hexane Extracts of Graptophylum pictum (L.) Griff Nurcholis, Waras; Artika, I Made; Hami Seno, Djarot Sasongko; Andrianto, Dimas; Aprianti, Apipah; Febrianti, Fina; Inawati, Inawati; Ratu, Antonius Padua; Arendra, Arya
Current Biochemistry Vol 1, No 2 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/58-65

Abstract

The species Graptophylum pictum (L.) Griff, also known as “daun ungu” in Indonesia, is a traditional herbaceous plant believed to have antidiabetic potential. The number of people in the world with diabetes has increased dramatically over the recent years. The treatment of type II diabetes is complicated by several factors inherent to the disease. Elevated postprandial hyperglycemia is one of the risk factors and the intestinal digestive enzyme α-glucosidase plays a vital role in carbohydrate metabolism. One of the antidiabetic therapeutic approaches which reduces the postprandial glucose level in blood is by the inhibition of α-glucosidase. In this study, phytochemical analysis, α- glucosidase inhibitory activity and enzyme kinetics of ethyl acetate- and hexane extracts of G. pictum were evaluated with the aim to analyze its antidiabetic potential. Phytochemical analysis revealed the presence of tannins, steroids, and alkaloids. Steroids were present in ethyl acetate extract but absent in hexane extract, while alkaloids were present in hexane extract but absent in ethyl acetate extract. The ethyl acetate and hexane extracts had 30.68 and 49.82 % inhibitory effect on α-glucosidase activity respectively. The kinetics of glucosidase enzyme of ethyl acetate and hexane extracts were determined by Lineweaver Burk plots. These exhibited uncompetitive and noncompetitive inhibition to alpha-glucosidase activity respectively. From the enzyme assay, we infer that ethyl acetate and hexane extracts of G. pictum contain potential α-glucosidase inhibitors that have the potential to be exploited for use in the treatment of diabetes
The Activity of Wungu Leaf (Graptophyllum pictum (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice Rahmi, Hayatul; Artika, I Made; Azwar, Norman Razief; Hami Seno, Djarot Sasongko; Nurcholis, Waras
Current Biochemistry Vol 1, No 2 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/83-88

Abstract

Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.
Characterization and Toxicity of Temulawak Curcuminoid Nanoparticles Riki, Riki; Kurniatin, Popi Asri; Ambarsari, Laksmi; Nurcholis, waras; Darusman, Latifah K
Current Biochemistry Vol 3, No 1 (2016)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/43-53

Abstract

Temulawak exctract contains curcuminoids which have anticancer potential. However, clinical application of curcuminoid has been limited due to its low bioavailability. One of the efforts that can be developed to solve this problem is incorporated curcuminoids into Solid Lipid Nanoparticles (SLN) carriers system. The objective of this study was to characterize dan evaluate anticancer potential of temulawak ethanolic fraction nanoparticles. HPLC method was used to determined curcuminoids content of temulawak ethanolic fraction. Characterization indicators like polydispersity index, particle size, morpholgy, and entrapment efficiency. HPLC chromatogram has shown of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found in temulawak ethanolic fraction. The particle size of nanoparticles obtained in this study was 648.4 ± 95 nm with polydispersity index value of 0.216. A uniform size distribution of nanoparticles as observed by Transmission Electron Microscopy (TEM). The entrapment efficiency of curcuminoid in nanoparticles was about 29.8%. Based on results of BSLT obtained temulawak extract Lethal Concentration (LC50) value of 213.24 ppm and 828.78 ppm of nanoparticles.
Hepatic Lipid Peroxide Profile on Inflamation Rats with Nanocurcuminoid Treatment Nurcholis, Waras; Chelsea, Chelsea; Ambarsari, Laksmi
Current Biochemistry Vol 4, No 1 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/50-62

Abstract

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Co-Authors Anggriawan, Made B. Antonius Padua Ratu, Antonius Padua Aprianti, Apipah Arendra, Arya Arifin, Poppy F. BAMBANG PONTJO PRIOSOERYANTO Bintang, dan Maria Chelsea, Chelsea DEWI SUKMA Dian Apriliani, Dian DIMAS ANDRIANTO Djarot Sasongko Hami Seno Edy Djauhari Purwakusumah Egra, Saat Eva Harlina Faiza, Lucky Lia Febrianti, Fina Fitria, Rizki Habibi, B Y Hami Seno, Djarot Sasongko Harmanto, Ning Hartanti Hartanti, Hartanti Hyakansa, Hyakansa I MADE ARTIKA Inawati Inawati, Inawati Inda Setyawati, Inda Irmanida Batubara Junardy, Fransiska Devy Kautsari, Sadwika Najmi KUNTO WIBISONO LAKSMI AMBARSARI latifah K Darusman Latifah K. Darusman M, Kornelia Rosvita MARIA BINTANG MARIA BINTANG Maria Magdalena Maulana, Tubagus Iqbal Maulidya, Innes Mohamad Rafi Mohamad Rafi Mono Rahardjo MUHAMAD SYUKUR Muhammad Agung Zaim Adzkiya Mustofa, Maily Muthoharoh, Rini Najmah Najmah, Najmah Nella Angelina Simanjuntak Norman Razief Azwar Novita, Rini Noviyanti, Lina Nur'aeni, Siti Nurul Khumaida P.Roswiem, Anna PERMASKU, GIA Popi Asri Kurniatin, Popi Asri Popy Asri Kurniatin Priyadi, Yogo Suro Puspita, Puspa Julistia Qomariyah, Eka Nurul Rahmi, Hayatul Ratna Puspita, Ratna Ridho, Rachmi RIKI RIKI, RIKI Rizal Damanik Rostantinata, Riski Roswiem, Anna Priangani Rusmiyati, Henny Sholeh, Muhammad Maftuchin Sri Suhesti Subroto, Erna Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taufik Ridwan, Taufik Tilaar, Kilala Toshisada Suzuki Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wisastra, Rosalina Wulan Tri Wahyuni Zamany, Nevianti