Articles

Found 17 Documents
Search
Journal : Alotrop

IDENTIFIKASI PROFIL FITOKIMIA DAN UJI AKTIFITAS ANTIOKSIDAN EKSTRAK DIETIL ETER DAUN SURIAN (TOONA SINENSIS (A.JUSS) M.ROEM) DENGAN METODE DPPH. Nurhamidah, Nurhamidah; Nurdin, Hazli; Manjang, Yunazar; Dharma, Abdi
Alotrop Vol 3, No 1 (2019)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Surian Toona sinensis (A.Juss) M. Roem is a plant that is widely used by the community in the field of medicine such as fever, diarrhea, blood booster, tonic and others. This study aims to determine the secondary metabolite compounds found in leaf extracts of Toona sinensis and test the antioxidant activity using the 2,2-diphenyl-1- (2,4,6-trinitrophenyl) -hydrazinyl (DPPH) method. Identification of secondary metabolites by alkaloids, terpenoids, steroids, flavonoids, phenolics, saponins, coumarin and carotenoids. Toona sinensis leaf was extracted using methanol as a solvent. The resulting extract was saponified with a KOH solution in methanol. The saponification results were extracted again with diethyl ether solvent. The ether layer obtained was washed with distilled water, then dried with Na2SO4 and evaporated to dry, then tested its antioxidant activity. The identification results show Surian leaves contain all secondary metabolites except alkaloids. The antioxidant activity parameters of the Surian extract (Toona sinensis) are indicated by the% inhibitory and IC50 values. Test results showed diethyl ether extract had moderate category of antioxidant activity with IC50 122, 3752 ?g / mL
PERBANDINGAN SENSITIVITAS NANOPARTIKEL PERAK DENGAN REDUKTOR ALBUMIN DARI TELUR AYAM DAN BEBEK UNTUK ANALISIS MERKURI Azhar, Fikri Fadillah; Elvinawati, Elvinawati; Nurhamidah, Nurhamidah
Alotrop Vol 3, No 2 (2019)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aims of this study were determine the protein level of chicken egg albumin (Gallus Sp.) And duck egg albumin (Anas domestica) also optimum conditions, sensitivity, and LOD values of silver nanoparticles (NPP) synthesized using bioreductors of chicken (Gallus Sp.) egg albumin and duck (Anas domestica) egg albumin for analysis of mercury ions (II). This research was conducted at the Laboratory of Chemistry FKIP UNIB on April - July 2019. Protein levels of chicken egg albumin and duck egg albumin were obtained in the amount of 0.210 gr / 100 ml and 0.234 gr / 100ml, respectively. The NPP synthesized in this study was carried out by mixing silver precursors derived from 10 mM AgNO3 solution and 1% albumin solution as bioreductors of each chicken egg albumin solution and duck egg albumin. The optimum condition of NPP synthesized using chicken egg albumin bioreductor and duck egg albumin was the same, with the number of comparisons between AgNO3 and albumin solutions namely 1: 2 and stirring time of 30 minutes. The results showed that NPP synthesized using chicken egg albumin and egg albumin ducks had good sensitivity to Hg metal with the addition of Hg metal concentrations above 50 ppm that be marked with significant color changes from brownish yellow to clear. LOD values in each NPP synthesized using bioreductors of chicken egg albumin and duck egg albumin were 3334 ppm and 2,238 ppm, so that NPP synthesized using bioreductor of duck egg albumin was more sensitive than NPP synthesized using bioreductor of chicken egg albumin for mercury metal analysis. This is influenced by the differences of protein levels of chicken egg albumin and duck egg albumin where the protein level of duck egg was higher than the protein level of chicken egg.
UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (CITROFORTUNELLA MICROCARPA) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI Oktasila, Deza; Nurhamidah, Nurhamidah; Handayani, Dewi
Alotrop Vol 3, No 2 (2019)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.
AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER BAKTERI ENDOFIT AKAR TANAMAN MORINGA OLEIFERA L (KELOR) Kuntari, Zeta; Sumpono, Sumpono; Nurhamidah, Nurhamidah
Alotrop Vol 1, No 2 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

[ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITE FROM ENDOFIT BACTERIA OF  Moringa oleifera L (KELOR) ROOTS]  The purpose of this research was aims to isolate and measure the ability of antioxidant activity from secondary metabolites produced by endophytic bacteria that grow in the live tissue root  of Moringa oleifera L. (kelor). Endophytic bacteria were purified and cultured using a solid  Murashige-skoog (MS)  medium for 3 days at room temperature. Secondary metabolites were obtained by centrifugation process at a rate of 3000 rpm for 20 minutes. The bacterial fermentation process  using  Nutrient Broth  (NB) medium for 72 hours with a shaker speed at 170 rpm . The suspension supernatant was extracted with a maceration method using 86% ethyl acetate, followed by vacuum rotary evaporator concentration at 40 ° C. The extract antioxidant activity test  was performed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method using a UV-Vis spectrophotometer at 517 nm wavelength and ascorbic acid as standard. The result of DPPH test showed that the antioxidant activity of ethyl acetate extract of endophytic bacterial from root of M. oleifera L root has IC50 value at  315, 396 ppm.  Based on these results, it can be concluded that the secondary metabolite extract of endophytic bacterial from M. oleifera L root classified as weak antioxidant (IC50> 250 ppm).
PEMANFAATAN EKSTRAK BUAH MORUS ALBA L. (MURBEI) SEBAGAI PENGAWET ALAMI IKAN SELAROIDES LEPTOLEPIS (SELAR) Nastiti, Diah Sari; Nurhamidah, Nurhamidah; Chandra, I Nyoman
Alotrop Vol 3, No 1 (2019)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study is to measure the influence of concentration and time of soaking of fruit extracts Morus alba l. (Mulberry) is effective as a preservative Selaroides leptolepis fish (Trevally). Samples taken of coral in the fruit of the Holy City of Argamakmur, North Bengkulu and trevally fish samples taken directly from the beach, the village Plow Jakat, province of Bengkulu. Fruit samples grinded using a blender and aqueous extracts are obtained through maceration method using aquades and filtered so that retrieved the mulberry and subsequent solution diluted with aquades be a concentration of 20, 40, 60 and 80% (v/v). Phytochemicals profile test to determination of secondary metabolites in extracts. The test is done through the methods of preserving this soaking on a several concentrations variation and soaking time with without soaking as control. The variation of the concentrations is done at a concentration of 10, 20, 30 and 40%, with soaking time variation 1, 2 and 3 hours. Variation of time observations were at 12, 18 and 24 hours and test the chemical content in the form of a test of water content, levels of Total Volatile Bases (TVB), and pH values. Phytochemical test results obtained the presence of saponins, alkaloids, terpenoids, phenolic and flavonoid compounds and do not contain steroids. The results showed that the extract of Mulberry fruits proved capable of lowering the pH and the levels of TVB but does not affect the water content of the fish. Soaking time variations do not affect the water content, pH and the levels of TVB. From the results obtained that the use of the mulberry fruit extract at concentrations of 30% with long submergence 1 hour most optimal for use as a preservative natural for S. leptolepis up to 18 hours at room temperature to the value pH 6.7 and TVB at 27.2 mg N%.
SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN BEBERAPA FRAKSI DARI KULIT BATANG JARAK (RICINUS COMMUNIS L.) Agustina, Wulan; Nurhamidah, Nurhamidah; Handayani, Dewi
Alotrop Vol 1, No 2 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

[PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF SOME FRACTIONS FROM BARK OF CASTOR (Ricinus communis L.)]. The Phytochemical screening was conducted to determine secondary metabolites found in the bark of castor (Ricinus communis L.).The test results of phytochemical screening that has been done presence of  phenolic, flavonoids, alkaloids, saponins, and terpenoids. Extraction is done by maceration using ethanol 96%. Tests performed on the fraction of the antioxidant activity of ethanol, ethyl acetate, n-hexane, and ascorbic acid as compared to using DPPH. The results of measuring the antioxidant activity using Uv-Vis Spectrophotometer IC50 values obtained succession namely fraction of ethanol, ethyl acetate, n-hexane, and ascorbic acid, 33,38, 24,38, 289, 05 and 12, 48 ppm. Fraction of ethanol and ethyl acetate has a very strong antioxidant activity due IC50<50 ppm while the n-hexane fraction very weak antioxidant activity. Phenolic and flavonoids the bark of castor that can be potentially as antioxidants
UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK DAUN Ricinus communis L (JARAK KEPYAR) Sarfina, Julia; Nurhamidah, Nurhamidah; Handayani, Dewi
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the antioxidant activity of jarak kepyar’s  leaf extracts (Ricinus communis L.) and to determine of the fraction the growth of Erwinia carotovora. Jarak kepyar’s leaf extracted by maceration method using ethanol and fractionated using n-hexane and ethyl acetate. Antioxidant test using DPPH (1,1-diphenyl-2-picrylhydrazyl) with various concentrations of 125 ppm, 100 ppm, 75 ppm, 50 ppm, 25 ppm, 15 ppm and 5 ppm. Antibacterial test conducted on the active fractions as antioxidants using paper disc method and variations in the concentration of 1000 ppm, 500 ppm, 250 ppm, 125 ppm, 62.5 ppm. Phytochemical test results jarak kepyar’s leaf contain secondary metabolites are alkaloids, flavonoids, phenols and terpenoids. The test results demonstrate the antioxidant activity of ethyl acetate fraction is very strong as an antioxidant with IC50 values of 5.4 ppm and a strong ethanol fraction categorized as antioxidants IC50 of 99.8 ppm. Antibacterial test results showed inhibition of the leaf fraction of  jarak kepyar’s (Ricinus communis L.) against Erwinia carotovora categorized weak growth in ethanol fractions with inhibition zone diameter of 4 mm and being in ethyl acetate fraction with a diameter of 5 mm
AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK DAUN Jatropha gossypifolia L Pangestu, Nia Sapitri; Nurhamidah, Nurhamidah; Elvinawati, Elvinawati
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study was conducted to determine the activity of Jatropha gossypifolia L (jarak merah’s) leaf extract as an antioxidant and antibactery against acne causing bacteria (Propionibacterium acnes). To obtain an J. gossypifolia L jarak merah’s leaf extract by maceration using 96% ethanol, and then fractionated with n-hexane and ethyl acetate. Testing of antioxidant activity by DPPH method and antibactery activity test by the paper disc method. Antioxidant activity test has done for ethanol fraction, ethyl acetate fraction and n-hexane fraction, while testing for antibacterial activity performed on fractions active as antioxidant. The test results demonstrate the antioxidant activity of ethyl acetate fraction and the ethanol fraction as a fraction very strong with IC50 value of 22.33 ppm for ethyl acetate fraction and 41.04 ppm for ethanol fraction. Then the fraction of ethyl acetate and ethanol fraction antibactery activity test, the result is known that both factions have the ability to inhibit the growth of bacteria Propionibacterium acnes demonstrated by the inhibition zone is formed. In 1000 ppm is formed diameter of the greatest inhibition zone that is equal to 6 mm for ethyl acetate fraction and 5 mm for ethanol fraction.
BIOSINTESIS NANOPARTIKEL PERAK MENGGUNAKAN EKSTRAK BUAH Passiflora flavicarva (MARKISA) UNTUK MENDETEKSI LOGAM BERAT Maryani, Dina; Firdaus, M. Lutfi; Nurhamidah, Nurhamidah
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this research was to synthesize silver nanoparticle (NPP) by using Passiflora flavicarva (Markisa) fruit extract as detector of heavy metal. The NPP particles produced were determined by their optimum condition on the ratio of the volume of  Passiflora flavicarva passion fruit extract to AgNO3 and the reaction time. The silver nanoparticles produced under optimum conditions will be tested for selectivity against several metals that are often present in the environment, once the selective metal is known then it can be sensitized to the metal using a UV-Vis spectrophotometer to determine the minimal concentrations that can be detected by the silver nanoparticles Formed. The results showed that Passiflora flavicarva passion fruit extracts could be used for the biosynthesis of  NPP particles because they contain vitamin C and flavonoids capable of reducing Ag+ to Ag0 in the form of NPP. The optimal conditions for forming NPP particles are in the 1: 2 ratio with a 2 hour reaction time. From the results of selectivity test of NPP to some heavy metals that often exist in environment, it is known that NPP selective to mercury metal. It was found that selective mercury was followed by a sensitivity test with a UV-Vis spectrophotometer, with sensitive NPP  up to a concentration of 6.7 ppm. From the results of this study it can be concluded that NPP particles formed can be used to detect mercury metal up to a minimum concentration of 6.7 ppm.
PEMANFAATAN ARANG AKTIF SABUT KELAPA SAWIT SEBAGAI ADSOBEN ZAT WARNA SINTETIS REACTIVE RED-120 DAN DIRECT GREEN -26 Puspita, Melfi; Firdaus, M. Lutfi; Nurhamidah, Nurhamidah
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The problem of environmental polution caused by waste of batik industry lately is increasing, so it needed a method to overcome that problem. The aim of this study was to determine the ability of activated charcoal from coconut fiber palm in adsorbing Reactive Red-120 and Direct Green-26 dyes in waste of batik along with determining the parameters of isotherms adsorption using UV-Vis Spectrophotometer analysis method. Variations of pH, contact time, adsorbent weight and temperature were carried out as variable to obtain optimum conditions of the adsorption process. The optimum of conditions for Reactive Red-120  occured at pH 3 and a contact time of 30 minutes, while Direct Green-26  occurred at pH 4 and a contact time of 40 minutes, with each adsorbent weight 150 mg and the temperature 30 °C. Adsorption isotherms determined by Freundlich and Langmuir models with maximum adsorption capacity (Qmax) were obtained for the Reactive Red-120  was 400 mg/g, while Direct Green-26 is 169 mg/g.