Arif Nurkanto
Research Center for Biology, Indonesian Institute of Sciences, Jalan Raya Bogor 46, Cibinong, Indonesia

Published : 16 Documents
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Journal : BERITA BIOLOGI

KERAGAMAN AKTINOMISETES KEPULAUAN WAIGEO, KABUPATEN RAJA AMPAT, PAPUA DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSADAN PELARUT FOSFAT Nurkanto, Arif
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1170.905 KB) | DOI: 10.14203/beritabiologi.v9i1.776

Abstract

Some Actinomycetes isolated from Waigeo Raja Ampat Regency Papua have been identified. Those isolates were also characterized for their cellulolitic and phosphate solubilizing ability. Microscopic identification was based on Miyadoh (1997) and Holt (1994) methods.Actinomycetes could be identified by microscopic observation on spores, chain spore, hypha, aerial hypha and its pigmentation.The cellulolitic ability was observed by clear zone ratio in CMC medium and phosphate solubilizing activity by the same approach in Vikoskaya medium.From 139 Waigeos Actinomycetes isolates which had been deposited in LIPI Microbial Collection (LIPIMC) were identified as 10 genera (Actinomadura, Actinoplanes, Microbiospora, Micromonospora, Nocardia, Pseudonocardia, Saccharopolispora, Streptomices, Streptosporangium and Thermomonospora). As much as 57.5% phosphate solubilizing actinomycetes and 82.7 % cellulolitic actinomycetes were detected.
KERAGAMAN AKTINOMISETES ASAL SERASAH, SEDIMEN, DAN TANAH PULAU ENGGANO, BENGKULU [Deversity of Actinomycetes From Soil, Sediment, and Leaf Litter Samples of Enggano Island, Bengkulu] Putri, Ade Lia; Nurkanto, Arif
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3223.38 KB) | DOI: 10.14203/beritabiologi.v15i3.2238

Abstract

A total of 344 isolates of actinomycetes were isolated from leaf litter and soil samples collected from four locations of Enggano Island, Bengkulu. eighty eight isolates were selected using morphological characteristics and identified using 16S rRNA gene sequence analysis. Three families (Micromonosporacea, Streptomycetaceae, and Streptosporangiaceae) and eight genera were found from these samples. Approximately 80% of these strains belonged to Streptomyces group and 20% of isolates were similar to rare actinomycetes. Based on homology search (BLAST) and phylogenetic tree analysis, isolates of rare actinomycetes (EgA15, EgA30, EgA85, EgA386, EgA236, EgA243, EgA335, EgA112, EgA41,EgA312, EgA314) were identified as Kitasatosporia paracochleata (100%), Kitasatospora azatica (99%), Kitasatospora griseola (99%), Sphaerisporangium album (99%), Actinoplanes nipponensis (99%), Pseudosporangium ferrugineum (99%), Nonomuraea rosela (99%), Nonomuraea guangzhouensis (98%), Micromonospora chaiyaphumensis (99%),Micromonospora chaiyaphumensis (99%), and Couchioplanes caeruleus (99%) respectively.
TOKSISITAS AKUT ORAL DUA SENYAWA BISANTRAKUINON (+)-2,2’-EPISITOSKIRIN A DAN (+)-1,1’-BISLUNATIN [ORAL ACUTE TOXICITY OF TWO BISANTHRAQUINONES (+)-2,2’-EPICYTOSKYRIN A AND (+)-1,1’-BISLUNATIN] Praptiwi, Praptiwi; Nurkanto, Arif; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1858

Abstract

Bisanthraquinones (+) - 2,2'-epicytoskyrin A and (+) -1,1'bislunatin produced by the endophytic fungus Diaporthe sp. GNBP-10 showed potent antibacterial activity on in-vitro test and have the opportunity to become new antibiotics candidates. The aspects of safety and toxicity of drug candidates have to be examined before applying to human. This study was conducted to determine the safety aspects of the compounds through acute oral toxicity testing in mice (Mus musculus). Acute toxicity of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin evaluated by the method of Up and Down Procedure with limit test at a dose of 2000 mg / kg. Results of acute toxicity test showed that the LD50 of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin were of 1638.87 mg / kg and > 2000 mg / kg respectively. Administration of (+)- 2,2'-epicytoskyrin A resulted in increased miliari multifocal hepatitis, fatty degeneration and necrosis of liver cells, and the renal tubule epithelial degeneration. Administration of (+) - 1,1'-bislunatin at a dose of 2000 mg / kg resulted in multifocal accumulation of inflammatory cells in the liver and degeneration of cells in the islets of Langerhans although not resulting in death. The administration of those compounds indicated the changes in the organs, but based on the UN/ECE classification of LD50 value showed that (+) 2,2'- epicytoskyrin A and (+) -1,1'-bislunatin included as low acute toxicity substance.
TOKSISITAS AKUT ORAL DUA SENYAWA BISANTRAKUINON (+)-2,2’-EPISITOSKIRIN A DAN (+)-1,1’-BISLUNATIN [ORAL ACUTE TOXICITY OF TWO BISANTHRAQUINONES (+)-2,2’-EPICYTOSKYRIN A AND (+)-1,1’-BISLUNATIN] Praptiwi, Praptiwi; Nurkanto, Arif; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1858

Abstract

Bisanthraquinones (+) - 2,2'-epicytoskyrin A and (+) -1,1'bislunatin produced by the endophytic fungus Diaporthe sp. GNBP-10 showed potent antibacterial activity on in-vitro test and have the opportunity to become new antibiotics candidates. The aspects of safety and toxicity of drug candidates have to be examined before applying to human. This study was conducted to determine the safety aspects of the compounds through acute oral toxicity testing in mice (Mus musculus). Acute toxicity of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin evaluated by the method of Up and Down Procedure with limit test at a dose of 2000 mg / kg. Results of acute toxicity test showed that the LD50 of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin were of 1638.87 mg / kg and > 2000 mg / kg respectively. Administration of (+)- 2,2'-epicytoskyrin A resulted in increased miliari multifocal hepatitis, fatty degeneration and necrosis of liver cells, and the renal tubule epithelial degeneration. Administration of (+) - 1,1'-bislunatin at a dose of 2000 mg / kg resulted in multifocal accumulation of inflammatory cells in the liver and degeneration of cells in the islets of Langerhans although not resulting in death. The administration of those compounds indicated the changes in the organs, but based on the UN/ECE classification of LD50 value showed that (+) 2,2'- epicytoskyrin A and (+) -1,1'-bislunatin included as low acute toxicity substance.
KERAGAMAN AKTINOMISETES KEPULAUAN WAIGEO, KABUPATEN RAJA AMPAT, PAPUA DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSADAN PELARUT FOSFAT Nurkanto, Arif
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.776

Abstract

Some Actinomycetes isolated from Waigeo Raja Ampat Regency Papua have been identified. Those isolates were also characterized for their cellulolitic and phosphate solubilizing ability. Microscopic identification was based on Miyadoh (1997) and Holt (1994) methods.Actinomycetes could be identified by microscopic observation on spores, chain spore, hypha, aerial hypha and its pigmentation.The cellulolitic ability was observed by clear zone ratio in CMC medium and phosphate solubilizing activity by the same approach in Vikoskaya medium.From 139 Waigeo's Actinomycetes isolates which had been deposited in LIPI Microbial Collection (LIPIMC) were identified as 10 genera (Actinomadura, Actinoplanes, Microbiospora, Micromonospora, Nocardia, Pseudonocardia, Saccharopolispora, Streptomices, Streptosporangium and Thermomonospora). As much as 57.5% phosphate solubilizing actinomycetes and 82.7 % cellulolitic actinomycetes were detected.
KERAGAMAN AKTINOMISETES ASAL SERASAH, SEDIMEN, DAN TANAH PULAU ENGGANO, BENGKULU Putri, Ade Lia; Nurkanto, Arif
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2238

Abstract

A total of 344 isolates of actinomycetes were isolated from leaf litter and soil samples collected from four locations of Enggano Island, Bengkulu. eighty eight isolates were selected using morphological characteristics and identified using 16S rRNA gene sequence analysis. Three families (Micromonosporacea, Streptomycetaceae, and Streptosporangiaceae) and eight genera were found from these samples. Approximately 80% of these strains belonged to Streptomyces group and 20% of isolates were similar to rare actinomycetes. Based on homology search (BLAST) and phylogenetic tree analysis, isolates of rare actinomycetes (EgA15, EgA30, EgA85, EgA386, EgA236, EgA243, EgA335, EgA112, EgA41,EgA312, EgA314) were identified as Kitasatosporia paracochleata (100%), Kitasatospora azatica (99%), Kitasatospora griseola (99%), Sphaerisporangium album (99%), Actinoplanes nipponensis (99%), Pseudosporangium ferrugineum (99%), Nonomuraea rosela (99%), Nonomuraea guangzhouensis (98%), Micromonospora chaiyaphumensis (99%),Micromonospora chaiyaphumensis (99%), and Couchioplanes caeruleus (99%) respectively.