Nunuk Aries Nurulita
Fakultas Farmasi Universitas Muhammadiyah Purwokerto Kampus 1 UMP, Jl. Raya Dukuhwaluh telp. (0281) 636751

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Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer Nurulita, Nunuk Aries; Meiyanto, Edy; Sugiyanto, .
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Gynura  procumbens  is  widely  used  as  traditional  remedy  in  South-East  Asia.  Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of  Gynura procumbens (FEG) and to investigate its cytotoxic properties and selectivity to colon cancerand breast cancer cancer cells. The chromatographic profile of FEG was determined using HPTLC densitometric  and  HPLC.  MTT  (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium  bromide) assay was performed to determine the growth inhibitory effect of FEG on the growth of WiDr, MCF-7, and T47D cells. NIH3T3, a normal cells was used to determine the selectivity of FEG, which  contained  small  amount  of  quercetin  as  identified  from  chromatographic  profile  both HPTLC  and  HPLC.  FEG  inhibited  cell  growth  of  WiDr,  of  MCF-7  and  of  T47D  cells  in  time dependent manner. Quercetin affected cell growth inhibition approximately two fold higher at WiDr and MCF-7, whereas FEG had lower effect on T47D cell. Quercetin did not seem as the main  active  compound  of  FEG.  At  this  study,  FEG  caused  less  inhibition  on  the  growth  of NIH3T3 cells than that of on all cell lines. Selectivity index (SI) of FEG on WiDr, MCF-7 and T47D were 4.97, 2.77 and 7.79 respectively. According to the datas obtained, FEG possesses moderate to high cytotoxicity properties on WiDr, MCF-7 and T47D cells. FEG demonstrates selective  effect  against  cancer  cells  and  reveals  prospective  properties  as  cancer chemoprevention agent.Keywords: Gynura procumbens, colon cancer, breast cancer, cytotoxicity, selectivity
EFFECT OF ETHANOLIC EXTRACT OF Annona muricata L SEEDS POWDER TO DECREASE BLOOD GLUCOSE LEVEL OF WISTAR MALE RATS WITH GLUCOSE PRELOAD Hasanah, Rian Ulva Rizki; Sundhani, Elza; Nurulita, Nunuk Aries
UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Abstract

Based on data from World Health Organization(WHO) diabetes mellitus was the direct cause of death of1.5 million people, more than 80% of deaths due todiabetes occurs in middle-income countries and low(WHO, 2014). According to the Basic Health Research(Riskesdas) in 2013, the incidence of diabetes mellitus inIndonesia increased by two times greater than in 2007.Annona muricata L is used as a traditionalmedicine to lower blood glucose levels with containtannin, phytosterols, calcium oxalate, alkaloids murisin(Arief and Hariana, 2006), flavonoids and essential oils(Surbakti, 1994). Flavonoid compounds have animportant role in the prevention of diabetes and itscomplications (Jack, 2012). Previous research showedthat bark of plants soursop has a most excellent efficacycompared with the roots and leaves of the Annonamuricata L in lowering blood glucose levels in male ratsinduced streptozozin (Rahmawati, 2014).This study aimed to examine the effect ofethanolic extract of Annona muricata L. seeds powder ondecreasing blood glucose level in wistar strain male ratwith glucose loading.
ANTIDIABETIC ACTIVITY OF POWDER AND ETHANOLIC EXTRACT OF ANTLION (Myrmeleon sp.) ON WISTAR STRAIN WHITE MALE RATS WITH GLUCOSE PRELOAD Rahma, Hamidah Haryanti; Sundhani, Elza; Nurulita, Nunuk Aries
UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Abstract

Diabetes mellitus (DM) is a group of metabolicdiseases characterized by hyperglycemia. It occursbecause abnormalities in insulin secretion, insulinaction, or both (Gustaviani, 2006). Diabetestreatment is generally done through regular insulininjections and delivery of oral antidiabetic drug suchas sulfonylurea class, biguanide, thiazolidinedion andmeglitinida which have some side effects likeheadache, dizziness, nausea, anorexia andpermanent organ damage. More over, they are alsoexpensive. People start taking treatment intraditional way natural materials, such as antlion(Myrmeleon sp.,) to lower blood glucose level (Lee etal., 2007).Previous research showed that the young antlionmethanolic extract of had the highest inhibitoryactivity toward α-glucosidase enzyme, was 22.81%(Faiz, 2008). Giving combination of Antlion andbananas for 14 days could decrease blood glucoselevels at a dose of 0,01ml / 200g (Tyas 2006).This study has examined the antidiabetic effects ofthe powder and ethanolic extract of antlion(Myrmeleon sp.) on blood glucose level of Wistarmale rats were burdened by glucose.
Gynura procumbens Prevents Chemoresistance through Inhibition MDR1 Expression on MCF-7 Breast Cancer Cell Line and Sensitizes the Cells to Doxorubicin Nurulita, Nunuk Aries; Meiyanto, Edy; Matsuda, Eishou; Kawaichi, Masashi
Indonesian Journal of Biotechnology Vol 17, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.238 KB) | DOI: 10.22146/ijbiotech.15998

Abstract

The long-term exposure of doxorubicin (Dox) causes enhancement in MDR1 expression that leads tobreast cancer cell resistance. This protein become a serious problem in cancer treatment and also well-knownas negative prognostic factor in breast cancer malignancies. The new approach using natural chemopreventivesubstance was developed to inhibit this resistance progress. This study was aimed to investigate whether ethylacetate fraction of Gynura procumnens (FEG) can prevent chemoresistance through suppressing the MDR1 proteinexpression. MCF-7 cell was used as chemoresistance cell model. The MCF-7 cells were maintained with 100nM Dox-contained medium for five weeks. The chemoprevention effect of FEG was investigated by treatedMCF-7/Dox with sub-toxic concentration of FEG. The cytotoxic properties of MCF-7 cells were determinedusing MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Immunofluorescenceand western blotting analysis was performed to detect the MDR1 expression. MCF-7/Dox cells need higherconcentration for inhibiting cell growth, were compared with MCF-7, shown by IC50value. The MDR1 proteinlevel elevated after Dox exposure in time dependent manner. The FEG treatment decreased MDR-1 proteinlevel with dose dependent manner. FEG in combination with DOX potentiates the DOX effect on breast cancercell growth inhibition. The FEG prevents the chemoresistance development in breast cancer cell line, MCF-7induced by Dox through inhibiting MDR1 expression. The additional of FEG enhances Dox effect on cell deathinduction. Thus, FEG could be developed as co-chemotherapy agent for reverse multidrug resistance
ISOLASI ETIL P-METOKSISINAMAT DARI RIMPANG KENCUR (Kaempferia galangal L.) DAN IDENTIFIKASINYA DENGAN KROMATOGRAFI GAS SPEKTROSKOI MASSA Caesaria, Cindy; Tjiptasusrasa, Tjiptasusrasa; Nurulita, Nunuk Aries
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 06 No. 02 Agustus 2009
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v6i2.420

Abstract

ABASTRAK Etil p-metoksisinamat (EPMS) adalah salah satu senyawa hasil isolasi rimpang kencur (Kaempferia galangal L.) yang merupakan bahan dasar senyawatabir surya yaitu pelindung kulit dari sengatan sinar matahari. Metode yang digunakan dalam penelitian ini adalah perkolasi. Setelah didapatkan perkolat selanjutnya dihilangkan pelarutnya hingga terbentuk Kristal dan selanjutnya dilakukan proses rekristalisasi. Hasil penelitian menunjukkan bahwa kemungkinan besar EPMS berada dalam ekstrak kencur yang diekstraksi menggunakan pelarut N-heksan dan mempunyai kelarutan dan kemurnian yang cukup tinggi yaitu sebesar 96,92%. Hal ini bisa dilihat dari kromatogram pada Kromatografi Gas Spektroskopi Massa yang menunjukkan 1 puncak yang sangat dominan. Kata kunci : EPMS, kencur (Kaempferia galanga L.), isolasi, KGSM. ABSTRACT EPMS – Ethyl p-methoxycinnamate was one of compound isolated from greater galingale rhizome (Kaempferia galanga L.) can be used as base substance of sun screen product. The method used in this research was percolation. The obtained percolate was dried until crystal was formed. The obtained crystal was later recrystallization. The result showed that greater galingale N-hexane extract possibly contained EPMS. The isolated substance had high solubility and purity (96,92%), it was seen from Gas Cromatograph Mass Spectrometer spectra that show one dominant peak. Keywords: EPMS, greater galingale (Kaempferia galanga L.), isolation, GCMS.
EFEK SITOTOKSIK DAN ANTIPROLIFERATIF KUERSETIN PADA SEL KANKER KOLON WiDr Kusuma, Arif Wirahadi; Nurulita, Nunuk Aries; Hartanti, Dwi
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 07 No. 03 Desember 2010
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v7i3.584

Abstract

Abstrak Kanker kolon merupakan penyakit salah satu penyakit yang banyak mengakibatkan kematian. Usaha penyembuhan kanker kolon melalui pembedahan kemoterapi dan radioterapi pada umumnya belum mampu memberikan hasil yang efektif. Hal ini mengakibatkan banyak dijumpai cara pengobatan alternatif antara lain menggunakan bahan dari alam. Salah satu bahan dari alam tersebut adalah kuersetin. Kuersetin merupakan senyawa golongan flavonoid yang dikenal memiliki efek antikanker. Penelitian ini bertujuan untuk mengetahui efek sitotoksik dan antiproliferatif kuersetin terhadap sel kanker kolon WiDr. Uji sitotoksik kuersetin dilakukan menggunakan metode MTT. Hasil Uji sitotoksik menunjukkan bahwa IC50 kuersetin terhadap sel WiDr adalah 1046 μM. Pengamatan kinetika proliferasi sel WiDr yang diberi perlakuan kuersetin menggunakan metode penghitungan langsung dilakukan pada jam ke 0, 24, 48 dan 72. Pada pengamatan morfologi sel, setelah dilakukan pengecatan menunjukkan adanya fenomena apoptosis pada sel kanker kolon WiDr. Hasil penelitian menunjukkan bahwa kuersetin memiliki efek sitotoksik dan antiproliferatif pada sel kanker WiDr. Kata Kunci : Kuercetin, WiDr, Sitotoksik, Antiproliferatif Abstract Colon cancer is one disease that many result in death. The of colon cancer cure through surgery chemotherapy and radiotherapy are generally not able to provide effective results. This caused many found ways to use alternative medicine among other materials from nature. One of these natural substances are quercetin. Quercetin is a flavonoid compounds are known to have anticancer effects. This study aimed to determine the effect of cytotoxic and antiproliferatif quercetin against colon cancer WiDr cells. Quercetin cytotoxic test was carried out using MTT method. Test results showed that the cytotoxic IC50 of quercetin against WiDr cells was 1046 μM. WiDr cell proliferation kinetics observation that quercetin treated using the method of direct calculation done at 0, 24, 48 and 72. In the observation of cell morphology, after the painting shows the phenomenon of apoptosis in colon cancer cell WiDr. The results showed that quercetin has cytotoxic and antiproliferative effects on cancer cells WiDr. Keywords : Quercetin, WiDr, Cytotoxic, Antiproliferative.
Uji Sensitivitas Antibiotik Terhadap Bakteri Penyebab Infeksi Saluran Kemih pada Pasien Rawat Inap di RSUD Prof. Dr Margono Soekarjo Purwokerto Muhammad, Adzkie; Nurulita, Nunuk Aries; Budiman, Arif
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 14 No. 02 Desember 2017
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v14i2.1684

Abstract

Penyakit infeksi masih merupakan penyebab kesakitan dan kematian yang tinggi di seluruh dunia, khususnya di negara berkembang seperti Indonesia. Di antara bakteri patogen, Escherichia coli merupakan bakteri paling umum menjadi penyebab infeksi saluran kemih pada pasien rawat jalan maupun rawat inap. Tujuan dari penelitian ini adalah mengidentifikasi jenis bakteri penyebab serta pola bakteri dan tingkat sensitivitas antibiotik terhadap bakteri penyebab infeksi saluran kemih (ISK) pada pasien rawat inap di RSUD Prof. Dr. Margono Soekarjo Purwokerto. Penelitian ini menggunakan dua jenis antibiotik yaitu asam pipemidat dan cefixime. Uji yang dilakukan meliputi uji hemosis, identifikasi bakteri secara biokimia, dan uji sensitivitas antibotik dengan metode difusi agar. Hasil uji identifikasi biokimia menunjukkan bahwa uji indol dan methyl red positif sedangkan uji Voges Proskauer (VP) dan sitrat hasilnya negatif. E. coli menurut Bergey manual menunjukkan hasil positif untuk uji indol dan methyl red sedangkan uji VP dan sitrat negatif. Diduga bakteri penyebab ISK pada penelitian ini adalah E. coli. Hasil uji sensitivitas bakteri terhadap antibiotik menunjukkan bahwa bakteri masih sensitif terhadap asam pipemidat sebesar 66,7% dan intermediet 33,3%. Antibiotik cefixime sebesar sensitif 55,6% dan intermediet 44,4%. Kedua antibiotik tersebut masih efektif digunakan untuk mengatasi ISK.
EFEKTIVITAS EKSTRAK ETANOL DAUN ADAM HAWA (Rhoeo discolor) DAN DAUN PUCUK MERAH (Syzygium campanulatum Korth.) DALAM MENURUNKAN KADAR GULA DARAH PADA TIKUS PUTIH JANTAN GALUR WISTAR DENGAN PEMBEBANAN GLUKOSA Sundhani, Elza; Syarifah, Della Caya Nur; Zumrohani, Lita Ratriyana; Nurulita, Nunuk Aries
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 13 No. 02 Desember 2016
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

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Abstract

Penderita diabetes melitus terus semakin meningkat seiring dengan pola hidup yang tidak seimbang. Diabetes melitus merupakan penyakit metabolik yang ditandai dengan peningkatan kadar gula darah di atas normal. Penelitian ini bertujuan untuk menguji efek ekstrak etanol daun adam hawa (Rhoeo discolor) dan daun pucuk merah (Syzygium campanulatum Korth.) terhadap penurunan kadar gula darah pada tikus putih jantan galur wistar yang dibebankan glukosa. Pada penelitian ini tikus jantan galur wistar dibagi menjadi 5 kelompok yaitu kontrol normal, kontrol positif (glibenklamida 0,6 mg/kg bb), kontrol negatif (CMC-Na), tiga kelompok ekstrak etanol daun adam hawa (dosis 100, 200, dan 400 mg/kg bb) dan tiga kelompok ekstrak etanol daun pucuk merah (dosis 300, 600, dan 1200 mg/kg bb. Data diperoleh dengan mengukur kadar gula darah tikus 30 menit setelah pemberian glukosa dan pada menit ke-30, 60, 90, dan 120 setelah perlakuan. Hasil uji penelusuran kandungan senyawa kimia menggunakan Kromatografi Lapis Lipis (KLT) dan pereaksi semprot menunjukkan adanya golongan senyawa flavonoid, alkaloid, dan terpenoid pada ekstrak daun adam hawa, sedangkan ekstrak etanol daun pucuk merah hanya mengandung flavonoid dan terpenoid. Hasil uji statistika menggunakan Anova dengan taraf kepercayaan 95% dan dilanjutkan dengan uji Post Hoc LSD menunjukkan bahwa tidak ada perbedaan yang bermakna antara ekstrak daun adam hawa (dosis 200 dan 400 mg/kg bb) dan ekstrak daun pucuk merah (300 dan 600 mg/kg bb) dengan glibenklamida (0,6 mg/kg bb) dalam aktivitasnya untuk menurunkan kadar glukosa darah tikus. Dosis rendah (100 mg/kg bb) pada adam hawa dan dosis tinggi pada pucuk merah (1200 mg/kg bb) tidak menunjukkan efek hipoglikemik pada tikus. Ekstrak adam hawa dan pucuk merah diduga mempunyai aktivitas antidiabetik yang tergantung dosis (dose dependent). Patients with diabetes mellitus are increasing with the behavior of an unbalanced life. Diabetes mellitus is a metabolic disease characterized by hyperglycemic. This study aims to evaluate the effect of adam hawa (Rhoeo discolor) and pucuk merah (Syzygium campanulatum Korth.) ethanolic leaves extract to decrease blood sugar levels on male wistar rats induced by glucose. Rats were divided into 5 groups: the normal control group, positive control group (glibenclamide 0.6 mg/kg), negative control group (CMC-Na), ethanolic extract of adam hawa group with doses of 100, 200, and 400 mg/kg bw and ethanolic extract pucuk merah group with doses of 300, 600, and 1200 mg/kg bw. Blood glucose levels were measured 30 minutes before and 30, 60, 120 minutes after per oral glucose induction. The results of phytochemical screening using Thin Layer Chromatography (TLC) shown ethanolic extract of adam hawa contained alkaloids, flavonoids, and triterpenoid, while ethanolic extract of pucuk merah only contained flavonoids and terpenoids. Glucose blood levels and AUC datas were statistically analyzed using Oneway Anova and continued with LSD. The datas shown no significant difference between the ethanolic extract of adam hawa (200 and 400 mg/kg bw) and pucuk merah (300 and 600 mg/kg bw) compared with that of glibenclamide (0.6 mg/kg bw) (p>0.05). The ethanolic extract of adam hawa at lower dose (100 mg/kg bw) and pucuk merah at higher dose (1200 mg/kg bw) did not exhibit hipoglicemic effect on rats. Both extracts seems to have antidiabetic properties with dose dependent manner.
EFEK ANTIANGIOGENESIS FRAKSI VI dan VII EKSTRAK METANOLIK KAYU SECANG (Cesalpinea sappan L.) Sugiarto, Imam Purnomo; Nurulita, Nunuk Aries; Hartanti, Dwi
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 07 No. 02 Agustus 2010
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v7i1.560

Abstract

ABSTRAK Penghambatan proses angiogenesis merupakan salah satu strategi menghambat perkembangan kanker. Penelitian ini bertujuan untuk mengetahui efek antiangiogenesis fraksi VI dan VII ekstrak metanolik kayu secang (Caesalpinia sappan L.) menggunakan model pembuluh darah pada mata tikus. Mata tikus diberi perlakuan bFGF (induktor angiogenesis) dan ekstrak metanolik kayu secang selama 8 hari selanjutnya diamati respon angiogenesisnya. Berdasarkan penelitian yang dilakukan didapatkan hasil ekstrak metanolik kayu secang mampu menghambat angiogenesis pada mata tikus. Respon angiogenesis untuk fraksi VI dosis 200, 400 µg/mL dan fraksi VII dosis 500, 1000 µg/mL masing-masing secara berurutan adalah sebesar (dalam %) 5 ± 7,07; 0 ± 0; 6,25 ± 8,83; 8,33 ± 0. Hasil ini menunjukkan bahwa fraksi VI dan VII ekstrak metanolik kayu secang memiliki efek antiangiogenesis. Kata kunci: antiangiogenesis, mata tikus, Caesalpinia sappan L. ABSTRACT Inhibition of angiogenesis has become a strategy to inhibit cancer development. The aim of the research is to investigate antiangiogenesis effect of 6th and 7th fractions of secang wood methanol extract (Caesalpinia sappan L.) in situ using rat eyes. Rat eyes were treated with b-FGF (angiogenesis inductor) and extracts during eight days to observe its angiogenesis response. The results showed that the secang wood methanol extracts could inhibit angiogenesis in rat eyes. Doses 200, 400 µg/mL of 6th fraction and doses 500, 1000 µg/mL of 7th fraction gave angiogenesis response of (in percent) 5 ± 7.07; 0 ± 0; 6.25 ± 8.83; 8.33 ± 0, respectively. These results indicate that the fractions have antiangiogenic effect. Key words: antiangiogenic, rat eyes, Caesalpinia sappan L.
The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L.) extract on T47D cell growth inhibition after 5-fluorouracil treatment Yuniarti, Azizah; Sundhani, Elza; Nurulita, Nunuk Aries
Pharmaciana Vol 8, No 2 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.279 KB) | DOI: 10.12928/pharmaciana.v8i2.9480

Abstract

5-Fluorouracil (5-FU) is chemotherapy agents that used in breast cancer treatment that has toxic effects such as myelotoxicity. its effectiveness relatively low. Bawang Dayak has been widely empirically used as a breast cancer treatment. This study's aims were to determine the sensitivity of 5-FU against T47D cells, as single and its combination with EBD, to determine apoptotic induction, and the inhibitory effect of T47D cell cycle. The cytotoxic assay was using the MTT method with the concetration of 5-FU (0-600μg / mL) and EBD (0-250μg / mL). Apoptotic induction analysis were determined by PI/annexin V. Both of apoptotic and cell cycle analysis was performed by flowcytometry. The results of the 5-FU and EBD cytotoxic test on T47D cells obtained IC50 274μg/mL and 75μg/mL, respectively. The combination of 5-FU and EBD has a mild to strong synergistic effect (CI 0.829 and 0.779). its ombined treatment induced apoptosis in T47D cells by 21.33%. Combination of these combinations caused cell accumulation in G0-G1 phase of 57.11%, greater than control (41.20%). Provision of 5-FU and EBD combination treatments causes disturbance and damage to cells that cause cell cycle arrest in the G0-G1 phase and can not proceed to the next phase. The combination of 5-fluorouracil and EBD has a synergistic effect as breast cancer co-chemotherapy is able to induce apoptosis against T47D cells by triggering cell accumulation in G0-G1 phase. The extract of Bawang Dayak has a high potential to be developed as co-chemotherapy in breast cancer.