ARIYANTI OETARI
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424

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PENGUJIAN KEMAMPUAN ANTAGONISTIK KHAMIR Rhodotorula spp. ASAL KEBUN RAYA CIBODAS TERHADAP KAPANG DARI TANAMAN TOMAT TERINFEKSI DENGAN CO-CULTURE Hafsari, Anggita R; Oetari, Ariyanti; Salamah, Andi; Sjamsuridzal, Wellyzar
JURNAL ISTEK Vol 5, No 1-2 (2011): ISTEK
Publisher : JURNAL ISTEK

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Abstract

Aspergilus ochraceu and A. terreus had been known as mould that causes significant loss to quality of tomatoes. One of potential methods to control the development of this mould by using the antagonistic activity of Rhodotorula sp. Six strains of Rhodotorula sp., UICC Y-318, UICC Y-325, UICC Y- 332, UICC Y-381, UICC Y-384, and UICC Y-386, which isolated from Cibodas Botanical Garden, West Java were observed for its antagonistic activity against mould A. ochraceu D1.2.2.2.SSM3 and A. terreus D2.2.MC. Co-culture method was used as 1.58 to 5.59 x 108 CFU/ml Rhodotorula sp. growth together with 7 x 107 CFU/ml A. ochraceus D1.2.2.2.SSM3 and 7.5 x 107 CFU/ml A. terreus D2.2.MC at PDB. Result showed that the highest percentage reduction of conidial heads and hyphal width was show by Rhodotorula sp. UICC Y-318 against A.ochraceus (9.45% and 12.43%; 7.10% and 7.51%, 2 and 3 day after incubation, respectively).
Penapisan Aktivitas Selulase Isolat-isolat Khamir dari Moluska, Serasah, dan Tumbuhan di Taman Nasional Gunung Halimun, Jawa Barat Mangunwardoyo, Wibowo; Fitri, Reno; Oetari, Ariyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.223 KB) | DOI: 10.24002/biota.v12i3.2805

Abstract

A total of 236 yeast isolates from mollusc, litter, and plant samples from Gunung Halimun National Park were screened for cellulolytic activity based on Smith method by using 0,2% (w/v) cellulose-azure for 30 days. The results showed that 12 isolates (9 isolates from plants, 2 isolates from molluscs, dan 1 isolate from litter) have cellulolytic activity. These isolates were further screened based on Teather and Wood method for six days to determine their cellulases components by using specific substrates. Carboxymethyl cellulose (CMC) 0,1% (w/v) was used as a specific substrate to determine endoglucanase activity. Avicel 0,1% (w/v) was used as a specific substrate to determine exoglucanase activity. Cellobiose 0,1% (w/v) was used as a specific substrate to determine ?-glucosidase activity. The results showed that 6 isolates from plant have ?-glucosidase activity, and 1 isolate from plant have ?-glucosidase and endoglucanase activities. Five isolates (2 isolates from plants, 2 isolates from molluscs, and 1 isolate from litter) showed no cellulase activity on specific substrates after six days incubation.