Soemitro Padmowijoto
Fakultas Peternakan, Universitas Gadjah Mada

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Utilization of mixed cellulolytic microbes from termite extract, elephant faecal solution and buffalo ruminal fluid to increase in vitro digestibility of King Grass Prabowo, Agung; Padmowijoto, Soemitro; Bachruddin, Zaenal; Syukur, Abdul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.38 KB) | DOI: 10.14334/jitv.v12i2.547

Abstract

Cellulose is a compound of plant cell walls which is difficult to be degraded because it composed of glucose monomers linked by β-(1.4)-bound. It will be hydrolysed by cellulase enzyme secreted by cellulolytic microbes. The effective digestion of cellulose needs high activity of cellulase enzyme. This research aims to increase in vitro king grass digestibility utilizing mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid. Twelve syringes contained gas test media were randomly divided into four treatments based on sources of microbe (SM), namely: S (SM: cattle ruminal fluid [S]), RGK (SM: mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid [RGK], with composition 1 : 1 : 1), S-RGK (SM: S + RGK, with composition 1:1), and TM (without given treatment microbe). Digestibility was measured using gas test method. Average of gas production treatment of S-RGK (70.2 + 0.6 ml) was higher and significantly different (P<0.01) compared to treatment of S (60.3 + 0.8 ml), RGK (40.8 + 2.3 ml), and TM (13.3 + 2.0 ml). Utilization of mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid (RGK) that combined with microbes of cattle ruminal fluid (S) could increase in vitro digestibility of king grass. Key Words: Cellulolytic Microbe, Termite Extract, Elephant Faecal, Buffalo Ruminal Fluid
EFFECT OF MIXING THE CALF FECES WITH COASTAL SANDYSOIL ON THE GROWTH OF CORN PLANT (Zea mays) Prabowo, Agung; Padmowijoto, Soemitro; Bachruddin, Zaenal; Syukur, Abdul
Widyariset Vol 14, No 2 (2011): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.375 KB)

Abstract

This research was intended to determine the effect of combination calf feces and coastal sandy-land on the growth of corn plant. This experiment was arranged in a 2x3x2 factorial pattern of completely randomized design. First factor was calf feces of with and without microbe treatment, second factor was three levels of feces incubation which were 0 day (I-0), 20 days (I-20), and 40 days (I-40), and third factor was fecal dosages of 15 ton/ha (D15) and 30 ton/ha (D30). Parameters of plant height, dry, and wet weight of roots and vegetative, chemical and physical properties of mixture of calf feces and coastal sandy-land were observed. The plant height was observed weekly. There was a signifi cant difference (P<0.05) on combination of feces without and with microbe treatment,fecal incubation time and dosage on the plant height. There was a tendency of increasing fecal dosage and fecal incubation time on the increase of plant height. This study suggests that calf feces could improve the corn plant growth in coastal sandy-land.
Utilization of mixed cellulolytic microbes from termite extract, elephant faecal solution and buffalo ruminal fluid to increase in vitro digestibility of King Grass Prabowo, Agung; Padmowijoto, Soemitro; Bachruddin, Zaenal; Syukur, Abdul
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.38 KB) | DOI: 10.14334/jitv.v12i2.547

Abstract

Cellulose is a compound of plant cell walls which is difficult to be degraded because it composed of glucose monomers linked by β-(1.4)-bound. It will be hydrolysed by cellulase enzyme secreted by cellulolytic microbes. The effective digestion of cellulose needs high activity of cellulase enzyme. This research aims to increase in vitro king grass digestibility utilizing mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid. Twelve syringes contained gas test media were randomly divided into four treatments based on sources of microbe (SM), namely: S (SM: cattle ruminal fluid [S]), RGK (SM: mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid [RGK], with composition 1 : 1 : 1), S-RGK (SM: S + RGK, with composition 1:1), and TM (without given treatment microbe). Digestibility was measured using gas test method. Average of gas production treatment of S-RGK (70.2 + 0.6 ml) was higher and significantly different (P<0.01) compared to treatment of S (60.3 + 0.8 ml), RGK (40.8 + 2.3 ml), and TM (13.3 + 2.0 ml). Utilization of mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid (RGK) that combined with microbes of cattle ruminal fluid (S) could increase in vitro digestibility of king grass. Key Words: Cellulolytic Microbe, Termite Extract, Elephant Faecal, Buffalo Ruminal Fluid