Maria Fatima Palupi
Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan, Gunungsindur, Bogor

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RESISTANSI ESCHERICHIA COLI TERHADAP KOLISTIN DAN DETEKSI GEN MOBILIZED COLISTIN RESISTANCE-1 PADA AYAM PEDAGING AKIBAT PEMBERIAN KOLISTIN SULFAT Palupi, Maria Fatima; Maheshwari, Hera; Darusman, Huda Salahuddin; Sudarnika, Etih; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.653 KB) | DOI: 10.19087/jveteriner.2018.19.2.196

Abstract

Colistin sulphate is the ultimate antimicrobial choice for the treatment of multidrug resistance gram negative bacteria infections with in human. The purposes of this study were to detect the presence of colistin resistant E. coli and mcr-1 gene in broiler and to transfer the mcr-1 gene to Salmonella enteritidis ATCC 13076. A total of 54 one day old broilers were divided into three groups that consists of 18 chicks broiler per group and raised up to 40 days old. The first group was used as control. The first treatment group was given colistin sulphate 5 ìg/g feed for 40 days and broilers in second treatment group was given 80.000 IU/kg body weight for first three days. Swab cloaca samples were taken every 10 days from each broiler. At age 40 days all chickens were slaughtered and meat samples were collected. Samples of cloacal swabs, fresh and cooked meat were examined for the presence of colistin resistant E. coli and mcr-1 gene. Susceptibility to colistin sulfate was conducted by agar dilution method, and detection of mcr-1 gene was conducted using polymerase chain reaction. The results showed that no colistin resistant E. coli was detected in the control group. Colistin resistant E. coli (27.78%) and mcr-1 gene (20.00%) were detected in animals in the first treatment group, respectively. Whilst 11.11% colistin resistant E. coli and 5.56% were carriying mcr-1 gene in the second treatment group. Colistin resistant E. coli were found 5.56% from raw meat samples and 3.70% had mcr-1 gene. Transfer of mcr-1 gene from colistin resistant E. coli to Salmonella enteritidis ATCC 13076 was success. These results showed the necessity of limitation usage of colistin sulphate in food animal. 
RISK ASSESSMENT OF THE USE OF COLISTIN SULFATE IN BROILER DUE TO ESCHERICHIA COLI RESISTANCE IN BROILER FLOCKS Palupi, Maria Fatima; Sudarnika, Etih; Wibawan, I Wayan Teguh; Darusman, Huda Shalahudin; Maheshwari, Hera
Acta VETERINARIA Indonesiana 2019: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.096 KB) | DOI: 10.29244/avi.0.0.14-23

Abstract

The risk assessment of antimicrobial resistance is very important to determine the risk of decreasing antimicrobial efficacy can be used as a basis for policymakers in allowing or prohibiting the use of an antimicrobial. This study aims to assess the risk of using colistin against E. coli resistance in the broiler flock. Risk assessment is carried out qualitatively using primary data, interviews, and secondary data. To obtain primary data various studies were carried out including monitoring the prevalence of colistin-resistant E. coli and mcr-1 also mcr-2 genes in broiler flocks, mcr-1 gene transfer from E. coli to Salmonella Enteritidis, mcr-1 gene sequencing, mutant selection windows of colistin against E. coli, and also multiresistant of E. coli colistin-resistant. Assessment of the risk of E. coli colistin-resistant in the broiler flocks through direct contact with live broiler flock environment with the resulting assessment is a medium risk with low uncertainty. Since colistin sulfate is very critically important for humans, the reduced use of colistin sulfate in animal production is necessary to reduce the risk of resistance. Reducing the use of colistin sulfate requires the collaboration of various sectors such as the government, veterinary drugs industries, farmers, and consumers.
VALIDATION OF THE SPECTROPHOTOMETRY METHOD ON THE CONCETRATION ASSESSMENT OF ENROFLOXACINE AS THE ANIMAL DRUG ., Ambarwati; Ariyani, Novida; Palupi, Maria Fatima
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.3815

Abstract

Abstract Validation for analysis of enrofloxacin injection method using spectrophotometry has been conducted. Enrofloksasin in a solution of NaOH (sodium hydroxide) 0.1 N, measured at the maximum wavelength (ë) 271 nm.The parameters of validation that were carried out are precision, accuracy, linearity, limit of detection and limit of quantitation. Precision showed good results with coefficien variation (CV) results smaller than that of CV Horwitz. Accuracy parameters calculated by the method of analysis to determine levels of analyte in a known active ingredient purity and yielded 0.449%. Linearity parameters showed good results with r = 0.9996. Limit of detection value was obtained for 0.757 ?g / mL and limit of quantification of 2.522 ?g / mL. The results of validation test of enrofloxacin injection analysis using spectrophotometric method can be used as a good method to provide a valid test of quality assurance veterinary drugs.