Articles

Found 1 Documents
Search
Journal : Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology

Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Crude and Fractionated Snakehead Fish (Channa striata) Fillet Extract Budiari, Setyani; Chasanah, Ekowati; Suhartono, Maggy Thenawidjaja; Palupi, Nurheni Sri
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.345

Abstract

The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead fish fillet is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous protein or peptide from snakehead fish fillet and to fractionate the active compounds using ultrafiltration. The extraction employed two solvents, i.e. aquadest and 50% ethanol. Fractionation was conducted using ultrafiltration membranes of 10,000; 5,000 and 3,000 Molecular Weight Cut Off  (MWCO) to separate the protein or peptide into the sizes of >10 kDa, 5-10 kDa, 3 -5 kDa and <3 kDa. The parameters observed were protein and peptide content, ACE inhibitory activity (in vitro) and also protein and peptide profiles. The result revealed that the snakehead fish fillet contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide of <10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of >10 kDa than the 50% ethanol. The fraction of <3 kDa by aquadest had the highest ACE inhibitor activity per g protein (7.85% inhibition of ACE per g protein). Thus, the fraction of <3 kDa aquadest is the most promising option for further research and development of natural anti-hypertension compound. From the result, snakehead fish fillet was potential to be utilized as a functional food as well as functional ingredient to fight hypertension.
Co-Authors - Lusianah . Komari . Subarna Ace Baekhari Aliwikarta, Kuswanto Aminudin, Aminudin Amiruddin Saleh Amrullah, - Andesko, Ros Maria Angka, Stephanie Asyhari, Muhammad Hasriandy Bambang Widuri Bremanti, Lisa Budi Nurtama Budi Suharjo Budiari, Setyani DAHRUL SYAH DEDDY MUCHTADI Dede Robiatul Adawiyah Didah Nur Faridah E. Srivishnu Herlambang Ekowati Chasanah Elvira Syamsir Endang Prangdimurti Febrianto, Yulizar Verda Feri Kusnandar Fransisca Fransisca FRANSISKA RUNGKAT ZAKARIA Gema Buana Putra Ghaisani, Mazaya Hakim, Shofia Nurul Hanifah Nuryani Lioe Harlen, Winda Christina Herpandi . Heryati Setyaningsih Iis Arifiantini Imam, Rosita Hardwianti Indrastuti, Nindya Atika Indrayana, Stefanus Komar Sumantadinata Larasati Ines Wardiani, Larasati Ines MADE ASTAWAN Maggy T Suhartono MAGGY THENAWIDJAJA SUHARTONO Mileiva, Steisianasari Muchtadi, Tien Ruspriatin Muhammad Alif Bardhani Muhammad Syamsun Musa Hubeis NANIK RAHMANI Nazli, Rizal Syarief Sjaiful Nelis Imanningsih Nur Sokib Nur Wulandari Nur, Rizaludin Nuri Andarwulan Ogawa, Masahiro Oke Anandika Lestari, Oke Anandika Perana, Ati Widya Primaniyarta, Mutiara Purwonugroho, Sigit Puspo Edi Giriwono Roserlina, Anita RTR, Ayu Puspitalena Rusdah Rusdah Sakinah, Ni'mawati Sari, Yana Nurita Sari, Yana Nurita Sibarani, Bernadetha Beatrix Siti Nurjanah Sparringa, Roy Alexander Sri Widowati Suhartono, Maggy Thenawidjaya Suratmono, Suratmono Suratmono, Suratmono Suratno, Yuhlanny Dewi Suseno, Rahayu TATI NURHAYATI Tjahja Muhandri Triana Setiawardani, Triana TUTIK WRESDIYATI Uju Victoria Valentina Winiati Pudji Rahayu YOPI YOPI Zhafira, Nida Raihana