Ida Parwati
Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung.

Published : 45 Documents
Articles

Comparison of Estimated Glomerular Filtration Rate Mean Value of HARUS 15-30-60, HADI, and ASIAN Fomula Accuracy in Diabetes Mellitus Type 2 Rachmayati, Sylvia; Parwati, Ida; Martakusumah, Abdul Hadi; Soelaeman, Rachmat; Sekarwana, Nanan
International Journal of Integrated Health Sciences Vol 3, No 1 (2015)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To compare the accuracy of HARUS 15-30-60, HADI, and Asian Formulas (Chinese-equation (Ch-E), Japanese-equation (Jp-E), and Thai- equation (Th-E)) for estimated glomerular filtration rate (eGFR).Methods: The Kidney Dialysis Outcome Quality Initiative (KDOQI) has published a guideline to measure renal function, which is based on glomerular filtration rate (GFR). This procedure is complicated and expensive, therefore an estimated GFR (eGFR) has been proposed. The modification of diet in renal disease (MDRD) study prediction equation is the most frequently eGFR used. This method still have a weakness in accuracy, so the chronic kidney disease epidemiology collaboration (CKD-EPI) formula is developed. Since CKD-EPI is not practical for daily use, the MDRD is published for Asian population that includes Ch-E, Jp-E, and Th-E. In Indonesia, the MDRD formula has not been validated using any gold standard, therefore 2 new formulas have been developed, i.e. HARUS 15-30-60 and HADI formulas. In this study, we analyzed 102 medical records of Diabetes Mellitus Type 2 (DMT2) patients who visited Dr. Hasan Sadikin General Hospital, Bandung during the period of 2012 to 2013. We analyzed the data using HARUS 15-30-60, HADI, Asian formulas, and then compared them to CKD-EPI to see the accuracy. Statistical analysis used was paired t-test in SPSS-17 program.Results: The accuracy of the different formulas are as follows: HADI (p=0.173), HARUS 15-30-60 (p=0.060), Ch-E (p=0.001), Th-E (p=0.000), and Jp-E (p=0.000).Conclusions: HADI is the most accurate formula, followed by HARUS Formula, Ch-E, and Th-E and Jp-E, respectively.Keywords: Chinese-equation, HADI and HARUS 15-30-60 formulas, Japanese-equation, and Thai-equation DOI: 10.15850/ijihs.v3n1.403
IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN SUNDARI, RINI; PARWATI, IDA; MOSE, JOHANES C.; SETIABUDIAWAN, BUDI
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Infection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and  intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune™ FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%.
Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia Rosana, Yeva; Sudiro, Tjahjani M.; Alisjahbana, Bachti; Parwati, Ida; van Crevel, Reinout; van Soolingen, Dick
Medical Journal of Indonesia Vol 14, No 3 (2005): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.775 KB) | DOI: 10.13181/mji.v14i3.186

Abstract

At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates. (Med J Indones 2005; 14: 142-6)Keywords: M. tuberculosis, DST, DSCP method
Efficiency of BacT/Alert in detecting Salmonella Parwati, Ida; Samaun, Endang
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

[no abstract available]
Multidrug resistance Salmonella in Dr. Hasan Sadikin General Hospital, Bandung Parwati, Ida; Samaun, Endang
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

no abstract available
Uji Validitas Neutrophil Gelatinase Associated Lipocalin sebagai Penanda Diagnosis Gangguan Ginjal Akut pada Sepsis Hidayat, -; Parwati, Ida; Gondodiputro, Rubin Surachno; Rita, Coriejati
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Gangguan ginjal akut (GgGA) merupakan penurunan fungsi ginjal secara mendadak yang ditandai dengan peningkatan kreatinin serum ≥0,3 mg/dL atau meningkat >1,5 kali dari kadar sebelumnya atau penurunan urine output (UO) <0,5 mL per jam selama >6 jam. Sepsis merupakan penyebab tersering GgGA dengan angka kejadian berkisar 20–50% dan angka kematian mendekati 70%. Kadar neutrophil gelatinase associated lipocalin (NGAL) urine penderita GgGA dapat meningkat secara cepat dan lebih awal dibandingkan dengan kadar kreatinin serum sehingga NGAL dapat dijadikan penanda diagnosis GgGA. Penelitian bertujuan mengetahui validitas NGAL urine sebagai penanda diagnosis GgGA pada penderita sepsis. Sebanyak 50 sampel urine diambil dari penderita sepsis di Unit Gawat Darurat (UGD), Intensive Care Unit (ICU), dan Medical Intermediate Care (MIC) di Rumah Sakit Dr. Hasan Sadikin Bandung selama Februari sampai Mei 2010 dan dilakukan pemeriksaan kadar NGAL urine dengan metode enzyme linked immunosorbent assay (ELISA). Data yang diperoleh dianalisis dengan uji nonparametrik Mann-Whitney, kurva receiver operating characteristic (ROC), dan uji validitas. Hasil penelitian didapatkan kadar NGAL urine penderita sepsis dengan GgGA lebih tinggi secara bermakna dibandingkan dengan penderita sepsis tanpa GgGA (3.380 ng/mL berbanding 116 ng/mL; p<0,001). Pada cut-off point 107 ng/mL, NGAL urine memiliki sensitivitas 100%, spesifisitas 36%, positive predictive value (PPV) 60,9%, negative predictive value (NPV) 100%, dan akurasi 68%. Simpulan, kadar NGAL urine memiliki validitas yang baik dan dapat dijadikan sebagai penanda diagnosis terjadinya GgGA pada penderita sepsis. [MKB. 2012;44(2):121–6]. Validity Test of Neutrophil Gelatinase Associated Lipocalin as Diagnostic Marker forAcute Kidney Injury on SepsisAcute kidney injury (AKI) is an abrupt decrease of renal function which marked by increase of serum creatinine ≥0.3 mg/dL or ≥1.5 times of previous level or decrease urine output <0.5 mL/hour in >6 hours. Sepsis is the most common cause of AKI with incidence rate is about 20–50% and mortality nearly 70%. Urine neutrophil gelatinaseassociated lipocalin (NGAL) level in AKI patients can increase quickly and earlier compared with serum creatinine and could be as a marker for AKI. The purpose of this study was to assess the validity of urine NGAL as diagnostic marker of AKI on sepsis patients. Subjects were 50 urine samples of sepsis patients from Emergency Department (ED), Intensive Care Unit (ICU) and Medical Intermediate Care (MIC) in Dr. Hasan Sadikin Hospital Bandung between February and May 2010 and were examined with enzyme linked immunosorbent assay (ELISA) method. Data analysis was performed by non parametric Mann-Whitney test, receiver operating characteristic (ROC) analysis and validity test.The results found that urine NGAL of AKI patients were significantly higher compared with non AKI patients (3,380 ng/mL vs 116 ng/mL, p<0.001). A cut-off point >107 ng/mL for urine NGAL had a sensitivity of 100%, specificity of 36%, positive predictive value (PPV) of 60.9%, negative predictive value (NPV) of 100% and accuracy of 68%. In conclusions, urinary NGAL level has good validity and could be used as a screening test for AKI on sepsis patients. [MKB. 2012;44(2):121–6]. DOI: http://dx.doi.org/10.15395/mkb.v44n2.131
Perbedaan Parameter Hematologi pada Penderita Tuberkulosis Paru Terinfeksi Mycobacterium Tuberculosis Galur Beijing dengan Galur Non-Beijing Sundari, Rini; Parwati, Ida; Mose, Johanes Cornelius; Setiabudiawan, Budi
Majalah Kedokteran Bandung Vol 49, No 1 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Tuberkulosis (TB) merupakan penyakit inflamasi kronik, tingginya kasus TB dapat disebabkan oleh perbedaan virulensi antargalur Mycobacterium tuberculosis (MTB). Penelitian ini bertujuan untuk menganalisis berbagai manifestasi hematologi yang terjadi pada penderita TB paru yang terinfeksi  galur Beijing dan non-Beijing MTB. Sampling penelitian dilakukan di Rumah Sakit Dr. H.A. Rotinsulu Bandung, RSU Cibabat Cimahi, Balai Besar Kesehatan Paru Masyarakat (BBKPM) Bandung, Puskesmas Batujajar, Puskesmas Padalarang, dan Puskesmas Cimareme pada Juni 2014–Januari 2015. Penelitian diikuti oleh 74 penderita TB paru BTA (+) terdiri atas 61% pria dan 39% wanita yang berusia 18–63 tahun. Berdasar atas spoligotyping diperoleh 24 (32%) terinfeksi galur Beijing dan 50 (68%) galur non-Beijing. Pemeriksaan laju endap darah (LED) menggunakan metode Westergreen, parameter hematologi lain menggunakan haematology analyzer. Kadar hemoglobin galur Beijing 8,6–14,8 g/dL dan galur non-Beijing 8,1–16,5 g/dL, anemia ini lebih banyak ditemukan pada penderita yang terinfeksi galur Beijing (17 dari 24) dibanding dengan galur non-Beijing 31 dari 50. Nilai absolut eritrosit tidak ada perbedaan, kecuali red blood cell distribution width (RDW). Hasil antara Beijing dan non-Beijing didapatkan hasil LED 94,0 (35,03) vs 89,9 (29,96) mm; leukositosis tidak berbeda namun 67% neutrofilia dan 17% limfopenia pada galur Beijing, 0% dan 30% pada galur non-Beijing; jumlah trombosit 46% (416,3+161,7)x1.000 sel/mm3 vs 122-834 (407,0+154,8)x1.000 sel/mm3 dengan trombositosis 63% vs 46%. Penderita terinfeksi galur Beijing menunjukkan anemia, LED, dan trombositosis lebih tinggi dibanding dengan non-Beijing; hal ini berarti penderita terinfeksi galur Beijing mengalami inflamasi yang lebih berat. [MKB. 2016;49(1):35–41]Kata kunci: Beijing, non-Beijing, profil hematologiThe Differences of Haematology Profile in Patients with Lung Tuberculosis Infected by Mycobacterium tuberculosis Beijing Strain and non-Beijing StrainTuberculosis (TB) is a chronic inflammation disease; a high numbers of tuberculosis cases can be caused by virulence potential of each Mycobacterium tuberculosis (MTB) strain. The event of inflammation process influences the hematopoietic system which gives various hematology examination results. This study was conducted in order to analyze various forms of hematological manifestation occur in patients with lung TB caused by MTB Beijing strain and non-Beijing strain infections.  This study was performed on 74 lung TB-infected patients with positive acid-fast bacilli, consisting of 61% males dan 39% females whose age ranged from 18 to 63 (32.6+12.2) years old. Spoligotyping was performed, resulting in 24 (32%) Beijing strain and 50 (68%) non-Beijing strain infections. Hematological examination was performed using hematology analyzer and erythrocyte sedimentation rate (ESR) with Westergreen method. Hemoglobin level ranged from 8.6 to14.8 (11.8) g/dL and 8.1-16.5 (12.0) g/dL from Beijing strain and non-Beijing strain, respectively, with more anemia was found in Beijing strain patients (71%) compared to non-Beijing strain (62%). There was no differences in absolute erythrocyte count, except in red blood cell distribution width (RDW).  The comparison of ESR result between Beijing and non-Beijing in ESR resulting in 94.0 (35.03) vs 89.9 (29.96) mm with no difference in leukocytosis, yet 66.7% neutrophilia and 16.7% lymphopoiesis in Beijing strain patients, 0% and 30% consecutively in non-Beijing strain. The number of thrombocyte is 68-882 (416.3+161.7)x1000 cells/mm3 vs 122–834 (407.0+154.8)x1000 cells/mm3 with thrombocytosis in 63% vs 46%. Beijing strain patients shows anemia, and higher ESR and thrombocytosis. These show that patients infected by Beijing strains experience more severe inflammation. [MKB. 2016;49(1):35–41]Key words: Beijing strain, non-Beijing strain, haematology profile
Validitas Metode Polymerase Chain Reaction GeneXpert MTB/RIF pada Bahan Pemeriksaan Sputum untuk Mendiagnosis Multidrug Resistant Tuberculosis Sirait, Nurlina; Parwati, Ida; Dewi, Nina Susana; Suraya, Nida
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Pengendalian tuberkulosis saat ini terkendala oleh metode diagnostik konvensional yang lambat terutama untuk mendeteksi multidrug resistant tuberculosis (MDR-TB). Deteksi dini MDR-TB sangat penting untuk mencegah penyebaran MDR-TB dan mengurangi angka kematian. Penelitian ini bertujuan menganalisis validitas pemeriksaan polymerase chain reaction genexpert MTB/RIF suatu pemeriksaan molekuler automatis yang cepat untuk mendeteksi MDR-TB. Penelitian dilakukan di Poliklinik Directly Observed Treatment Short- Course (DOTS) Rumah Sakit Dr. Hasan Sadikin Bandung. Subjek penelitian adalah penderita tersangka MDRTB. Sampel penelitian berupa dahak yang dilakukan pemeriksaan mikroskopis, uji kepekaan metode proporsi media Lowenstein Jensen dan polymerase chain reaction genexpert MTB/RIF. Selama periode Agustus 2012 sampai Januari 2013 didapatkan 88 subjek penelitian, kelompok usia terbanyak 25–34 tahun. Hasil pemeriksaan kultur didapatkan 54 sampel tumbuh, 34 sampel tidak tumbuh, dan 3 sampel merupakan Mycobacteria lain dari tuberkulosis. Sebesar 97,5% sampel yang resisten rifampisin juga resisten terhadap isoniazid. Pemeriksaan GeneXpert MTB/RIF menunjukkan sensitivitas 92,3% dan spesifisitas 75% dengan akurasi 88,2%. Simpulan, pemeriksaan GeneXpert MTB/RIF memiliki validitas tinggi untuk mendiagnosis MDR-TB terhadap baku emas uji kepekaan M. tuberculosis metode proporsi pada media Lowenstein Jensen. Pemeriksaan ini disarankan sebagai alat skrining MDR-TB. [MKB. 2013;45(4):234–9]Kata kunci: GeneXpert MTB/RIF, MDR-TB, uji kepekaan metode proporsiValidity of Polymerase Chain Reaction GeneXpert MTB/RIF Method on Sputum Sample Examination to Diagnose Multidrug Resistant TuberculosisControl of tuberculosis is hampered by slow conventional diagnostic methods especially for the detection of multidrug resistant tuberculosis (MDR-TB). Early detection of MDR-TB is essential to interrupt MDR-TB transmission and reduce the death rate. The aim of this study was to assess the validity of polymerase chain reaction genexpert MTB/RIF examination, which is a rapid automated molecular examination for the detection of MDR-TB. The study was conducted at the Directly Observed Treatment Short-Course (DOTS) clinic at Dr. Hasan Sadikin General Hospital Bandung. Subjects were patients with suspected MDR-TB. The research sample was sputum which was subjected to microscopic examination, susceptibility test proportion methods in Lowenstein Jensen media and polymerase chain reaction genexpert MTB/RIF examination. During August 2012 until January 2013, 88 subjects were obtained, with most of them were 25–34 years old. There were 54 samples obtained that grew in culture and 34 samples did not grow while 3 samples were other Mycobacteria of tuberculosis. It was also found that 97.5% of rifampin-resistant samples were also resistant to isoniazid. Examination of GeneXpert MTB/RIF showed a sensitivity of 92.3%, specificity of 75% with an accuracy of 88.2%. In conclusion, GeneXpert MTB/RIF examination has high validity for diagnosing MDR-TB against M. tuberculosis gold standard resistance test using the proportion method in Lowenstein Jensen media. The examination is recommended for MDR-TB screening. [MKB. 2013;45(4):234–9]Key words: GeneXpert MTB/RIF,MDR-TB, susceptibility test proportion methods DOI: http://dx.doi.org/10.15395/mkb.v45n4.170
Clinical and Cerebrospinal Fluid Abnormalities as Diagnostic Tools of Tuberculous Meningitis Lestari, Fiona; Dian, Sofiati; Parwati, Ida
Althea Medical Journal Vol 3, No 1 (2016)
Publisher : Althea Medical Journal

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Abstract

Background: Tuberculous meningitis (TBM) is the most severe form of extrapulmonary tuberculous (TB) disease and remains difficult to diagnose. The aim of the study was to determine the diagnostic value of clinical and laboratory findings of cerebrospinal fluid (CSF) examinations for diagnosing TBM using bacterial culture result as the gold standard.Methods: A prospective cross sectional study was carried out to 121 medical records of hospitalized TBM patients in neurological ward at Dr. Hasan Sadikin General Hospital Bandung, from 1 January 2009–31 May 2013. The inclusion criteria were medical records consisted of clinical manisfestations and laboratory findings. The clinical manisfestations were headache and nuchal rigidity, whereas the laboratory findings were CSF chemical analysis (protein, glucose, and cells) and CSF microbiological culture. Validity such as sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) for clinical and laboratory findings were calculated, using bacterial culture result as the gold standard.Results: The most clinical findings of TBM was nuchal rigidity and it had the highest sensitivity value, but the lowest spesificity value. Decreased of CSF glucose had the highest sensitivity value compared to other laboratory findings, but the value was low.Conclusions: The clinical manisfestations and the laboratory findings are not sensitive and specific enough for diagnosing TBM. [AMJ.2016;3(1):132–6] DOI: 10.15850/amj.v3n1.725
Validitas Multiplex Real Time Polymerase Chain Reaction untuk Diagnosis Limfadenitis Tuberkulosis pada Spesimen Blok Parafin Rezeki, Mike; Parwati, Ida; Hernowo, Bethy S.; Tjandrawati, Anna
Majalah Kedokteran Bandung Vol 46, No 3 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Limfadenitis tuberkulosis merupakan tuberkulosis ekstraparu yang paling sering ditemukan. Saat ini baku emas diagnosis limfadenitis tuberkulosis berdasarkan histopatologi. Pemeriksaan histopatologi tidak memberikan informasi etiologi pasti penyebab limfadenitis, padahal limfadenitis dapat disebabkan Mycobacterium tuberculosis maupun Mycobacterium non-tuberculosis yang sangat berbeda regimen terapinya. Spesimen limfadenitis tuberkulosis dalam blok parafin yang disimpan sangat bermanfaat ketika dibutuhkan pemeriksaan lanjutan.  Pemeriksaan multiplex real time polymerase chain reaction  pada  blok parafin dapat mendeteksi Mycobacterium  tuberculosis atau Mycobacterium  non-tuberculosis. Pemilihan objek penelitian dilakukan berdasarkan randomisasi sederhana. Penelitian uji diagnostik dengan  rancangan potong lintang untuk mengetahui  validitas pemeriksaan multiplex  real time polymerase chain reaction  dalam  mendiagnosis limfadenitis tuberkulosis  sebagai konfirmasi pada blok parafin dilakukan periode Juni 2012˗˗Juni 2013 di Rumah Sakit Dr. Hasan Sadikin Bandung. Objek penelitian terdiri atas 40 blok parafin limfadenitis tuberkulosis dan 22 blok parafin kelompok kontrol. Pemeriksaan multiplex real time poymerase chain reaction menggunakan primer gen sikuens sisipan IS6110 dan gen Mycobacterium protein tuberkulosis MPB64 untuk mendeteksi deoxyribonucleic acid Mycobacterium tuberculosis dan gen 16S rRNA (ribosomal ribonucleic acid) untuk mendeteksi Mycobacterium non-tuberculosis. Hasil uji validitas sensitivitas 75%, spesifisitas 77%, nilai duga positif 86,6%, nilai duga negatif 63%, dan akurasi 75,8%. Pemeriksaan multiplex real time polymerase chain reaction dapat dianjurkan untuk konfirmasi diagnosis limfadenitis tuberkulosis pada blok parafin. Simpulan: multiplex real time polymerase chain reaction  memiliki validitas sedang untuk diagnosis limfadenitis tuberkulosis pada spesimen blok parafin.Kata kunci: Deoxyribonucleic acid, limfadenitis tuberkulosis, multiplex real time polymerase chain reaction, Mycobacterium tuberculosis Validity of Multiplex Real Time Polymerase Chain Reaction for Tuberculous Lymphadenitis Diagnosis on Formalin Fixed Paraffin EmbeddedTuberculous lymphadenitis is one of the most common form of extra pulmonary tuberculous. The diagnosis of tuberculous lymphadenitis based  on  histopathology as a gold standard. Histopathology examination could not inform the etiology of lymphadenitis whether Mycobacterium tuberculosis or Mycobacterium nontuberculosis  of which  the treatment regiment is very different. The archieved specimen like formalin fixed paraffin embedded  of tuberculous lymphadenitis is useful for futher investigation. The multiplex real time polymerase chain reaction  can detect  Mycobacterium tuberculosis or Mycobacterium nontuberculosis  from formalin fixed paraffin embedded. The object of the study use simple random. The study with cross sectional design  to determine validity multiplex real time polymerase chain reaction  on tuberculous lymphadenitis from formalin fixed paraffin embedded, was done June 2012˗˗June 2013 at Dr. Hasan Sadikin Hospital Bandung. Objects  consisted  of  40  formalin fixed paraffin embedded  tuberculous lymphadenitis and  22 control group. The multiplex real time polymerase chain reaction use insertion  sequence IS6110 and Mycobacterium protein tuberculosis MPB64 gen for detect deoxyribonucleic acid Mycobacterium tuberculosis and 16S rRNA (ribosomal ribonucleic acid) gen for Mycobacterium nontuberculosis. The validity test of the Mycobacterium tuberculosis multiplex real time polymerase chain reaction with histopathology as a gold standards gave a sensitivity 75%, specificity 77%, positive predictive value 86.6%, negative predictive value 63%, and accuracy 75.8%. This examination can be used for confirmation of tuberculous lymphadenitis from formalin fixed paraffin embedded. Conclussion: multiplex real time polymerase chain reaction has intermediate validity to diagnose tuberculous lymphadentis on formalin fixed paraffin embedded.Key words: Deoxyribonucleic acid,  multiplex real time polymerase chain reaction, Mycobacterium tuberculosis DOI: 10.15395/mkb.v46n3.314
Co-Authors - Hidayat, - - Liza - Noormartany Abdul Hadi Martakusumah Agnes Rengga Indrati Ahmad Faried Andi Basuki Prima Birawa Anggraini Alam, Anggraini Ani Melani Maskoen Anna Tjandrawati AR, Ganiem Arto Yuwono Soeroto, Arto Yuwono Bachti Alisjabana, Bachti Bachti Alisjahbana banowati, marni Basti Andriyoko Bayuaji, Hartanto Bethy S. Hernowo Bethy Suryawati Hernowo Budi Setiabudiawan Budiman, Astrid Tamara Maajid Catarina, Erica Chrysanti Murad, Chrysanti Cissy B Kartasasmita Cissy B. Kartasasmita Coriejati Rita Dahlan, Zulkarnain Dewi Kartika Turbawaty Dewi Nurhayati Dick van Soolingen Djatnika Setiabudi DK, Turbawati Edhyana Sahiratmadja Effendi, Yusuf Sulaiman Efrida Efrida, Efrida Endang Samaun Endang Sutedja Fairuz Quzwain, Fairuz Fiona Lestari, Fiona Gunawan, J. E. Gurnida, Dida Ahmad Hadyana Sukandar Hartantri, Yovita Hendra Subroto, Hendra Herri S. Sastramihardja Herry Garna I, Agnes Rengga Ike Sri Redjeki Indahwaty, - Ivana Agnes Sulianto, Ivana Agnes Johanes C. Mose Johanes Cornelius Mose Juniati, Rianita Jupri, Erna Larissa, Larissa Lelly Yuniarti, Lelly Leni Lismayanti Livia Noviani, Livia Mega, Gde Sindu Meijerink, Hinta Mike Rezeki Nafsi, Nurizzatun Nafsi, Nurizzatun Nanan Sekarwana Nataprawira, Heda M Nenny Gustiani Ni Sayu Dewi B. Nida Suraya Nina Susana Dewi Nina Tristina Nurlina Sirait Oktavia, D Prayudi Santoso Prihatni, Delita Prihatni, Delita Pujiyanti, Hapsari Rachmat Soelaeman Rachmayati, Silvia Reinout van Crevel Rengga Indrati, Agnes RINI SUNDARI, RINI Rita Rachmayanti, Rita Rizal, A Roesli, Rully MA. Rovina Ruslami, Rovina Rubin Surachno Gondodiputro Rudi Wisaksana Sjahid, I. Sjahid, Idaningroem Soeroto, Arto Y Sofiati Dian, Sofiati Sugianli, A K Sugianli, Adhi Kristianto Sumantri, Rahmat Suryana, Nida Sylvia Rachmayati T, Dewi Kartika Tiene Rostini, Tiene Tjahjani M. Sudiro van der Venn, Andre Wahyudi, Kumia Wida Purbaningsih, Wida Wulan Ardhana Iswari, Wulan Ardhana Yani Triyani, Yani Yanti Yanti Yeva Rosana Yulius, Elsa Yuwono, Arto