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KAJIAN PATOGENISITAS BAKTERI EDWARDSIELLA ICTALURI PADA IKAN PATIN PANGASIONODON HYPOPHTHALMUS Susanti, Wiwik; Indrawati, Agustin; Pasaribu, Fachriyan H
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3460.241 KB) | DOI: 10.19027/jai.15.99-107

Abstract

ABSTRACT One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty individu of five group fishes, 6?10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P<0,05) compared to controls. LD50 dose was 2,8×104 cfu/mL. The result indicated that E. ictaluri was very pathogenic on striped catfish P. hypophthalmus.  Keywords: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), pathogenicity, striped catfish  ABSTRAK Salah satu kendala yang dijumpai pada budidaya ikan patin Pangasionodon hypophthalmus yaitu serangan penyakit bakterial. Enteric septicemia of catfish (ESC) adalah penyakit infeksi bakteri Edwardsiella ictaluri yang dapat menyebabkan kematian ikan patin sampai >50%. Penelitian ini dilakukan untuk mengetahui patogenisitas E. ictaluri isolat lokal pada ikan patin. Masing-masing 30 ekor ikan patin ukuran 6?10 g/ekor diinjeksi secara intraperitoneal dengan 0,1 mL larutan bakteri kepadatan 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; dan PBS sebagai kontrol. Ikan dipelihara selama tujuh hari pada akuarium berukuran 60×40×45 cm3. Pemeriksaan makroskopis dan mikroskopis dilakukan terhadap organ eksternal (kulit dan abdomen) dan internal (hati, ginjal, dan otak). Pengambilan sampel organ internal untuk uji histopatologi pada hari kelima dan sampel darah untuk uji gambaran darah pada hari pertama, ketiga, dan kelima pascainfeksi. Jumlah kematian ikan dihitung untuk mendapat nilai LD50. Pengamatan gejala klinis dan patologi anatomi ditemukan ikan berenang vertikal, adanya bercak merah pada kulit, pembengkakan abdomen, asites, hati pucat, dan ginjal berwarna merah kehitaman. Hasil histopatologi terlihat terjadinya degenerasi hidropik, degenerasi lemak, melano macrophage center (MMC), nekrosa, hemoragi, dan infiltrasi sel radang pada hati, ginjal, dan otak. Penurunan nilai hematokrit dan hemoglobin pada perlakuan secara statistik berbeda nyata (P<0,05) dengan kontrol. Dosis LD50 didapat 2,8×104 cfu/mL. Hasil penelitian ini mengindikasikan bahwa E. ictaluri pada ikan patin bersifat sangat patogen. Kata kunci: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), patogenisitas, patin
Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer Saputri, Dina Dyah; Bintang, Maria; Pasaribu, Fachriyan H
Current Biochemistry Vol 2, No 2 (2015)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/86-98

Abstract

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.
Antimicrobial activity and identification of bioactive compounds of Söfö-söfö (Acmella cf) leaf extract using GC-MS bintang, Maria; Bawamenewi, Faoziduhu; Safithri, Mega; Pasaribu, Fachriyan H
Current Biochemistry Vol 3, No 2 (2016)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/102 - 111

Abstract

Söfö-söfö is a traditional medicinal plant from Nias Island that can cure fever, cough, diarrhea and fungal infections on the skin. However, the scientific basis of these plants is unknown. The aim of this research was to extract the Söfö-söfö leaf by maceration method using two solvents that were 70 % ethanol and ethyl acetate, to test the antimicrobial activity of the extract by Agar diffusion method against Staphylococcus aureus, Eschercia coli, and Candida albican, and analyze secondary metabolite compounds by fitochemistry test and to determine the components of bioactive compounds by GC-MS. The results showed that the best solvent for making Söfö-söfö extract as antimicrobial is ethyl acetate with a minimum inhibitory concentration of 4000 ppm to S. aureus (1.25 ± 0.35 mm), E. coli (1 mm) and C. albican 6000 ppm (1.5 mm). The secondary metabolite compounds of ethyl acetate extract were alkaloids, flavonoids and steroids. Bioactive compounds found in the Söfö-söfö ethyl acetate extract with potential antimicrobial activity were hexadecanoic acid, stigmasterol, neophytadiene, methyl ester, squalene and phytol.
RELATION BETWEEN ENCAPSULATION OF STREPTOCOCCI OF SEROLOGICAL GROUP B AND ADHERENCE PROPERTIES OF THE BACTERIA TO DEAE-SEPHACEL Lammler, Christoph; Wibawan, I Wayan Teguh; Pasaribu, Fachriyan H
Media Veteriner Vol. 5 No. 4 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (758.324 KB)

Abstract

Determination of surface charge of group B streptococci by ion exchange chromatography on DEAE-Sephacel revealed that bovine and human group B streptococcal isolates with protein surrface antigens alone, or bacteria with protein antigen in combination with polysacharide antigens, adhered strongly to the gel matrix. In contrary, cultures with polysacchaide antigens alone showed no comparable adherence properties. Removal of neuraminic acid from bacterial surface enhanced, but pronase treatment reduced the adherence values. The importance of type specific capsular sialylation for group B streptococcal surface charge could be confirmed with group B streptococci of serotype III and their transposon mutagenized asialocapsular mutants. In contrary to the encapsulated parent strains the asialo capsular mutants adhered strongly to the gel matrix. Comparable differences were observed with unencapsulated group B streptococcal variant strains and its isogenic encapsulated parent strains. The capsule material seemed to mask the surface proteins responsible for the adherence to the gel matrix. The determination of surface charge of group B streptococci by ion exchange chromatography might help to understand the importance of capsular sialylation for individual isolates of this bacterial species.