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BRUCELLOSIS SEROPOSITIVITY IN SHEEP SLAUGHTERED AT SMALL RUMINANT SLAUGHTERHOUSE IN BOGOR REGENCY Septiningtyas, Widya; Pribadi, Eko Sugeng; Pasaribu, Fachriyan Hasmi
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8095

Abstract

Brucellosis is among the important diseases in livestock because the disease infects multiple species of animals and causes economic loss. Brucellosis in sheep is generally caused by Brucella melitensis and/or Brucella ovis. This study aimed to detect seropositive brucellosis in sheep. Serological tests used in this study was a parallel test between Rose Bengal Test (RBT) and Complement Fixation Test (CFT). Samples were collected from 150 sheep slaughtered in small ruminant slaughterhouse, Sentul, Bogor Regency. Seropositive proportion of brucellosis in sheep based on parallel test RBT and CFT was 52% (78/150).
CROSS REACTION OF SERUM IN SALMONELLA ENTERITIDIS- VACCINATED CHICKEN TO SOME SALMONELLA ENTERICA SEROTYPES (REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) Arnafia, Wyanda; Ningrum, Siti Gusti; Puspita, Erfiandini Eka; Lukman, Denny Widaya; Pasaribu, Fachriyan Hasmi; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Salmonella spp. has been recognized as the major cause of food-borne illness in humans worldwidecausing remain relevant to public health. Poultry vaccination is one promising strategy to mitigateSalmonella infection in poultry and, in turn, in humans as well. The objective of this study was to assessthe potential of cross-reaction of serum in Salmonella enteritidis-vaccinated chicken to some serotype ofSalmonella enterica. Four female, Isa Brown layer chickens (20 weeks old), were vaccinated with S. enteritidisstrain Sm24/Rif12/Ssq (intra vena) to induced the production of specific antibodies in serum. Crossreactionof serum in S. enteritidis-vaccinated chicken were assess with agar gel immunodiffusion test(AGID) with S. enteritidis, S. pullorum, S. typhimurium, S. typhi, and Escherichia coli antigens. Serumcould react with S. enteritidis and all types of S enterica used in this study (S. pullorum, S. typhimurium,S. typhi), but could not react with E. coli. The potential of cross-reaction of serum in S. enteritidis-vaccinatedchicken to some serotypes of S. enterica may play a role in reducing the infection caused by that serotype.
HEMAGGLUTINATION ACTIVITIES AND PHENOTYPIC EXPRESSIONS OF FIFTY-FIVE S. AGALACTIAE ISOLATES CONSISTED OF 19 STANDARD AND 36 FIELD ISOLATES FROM SUBCLINICAL MASTITIS CATTLE WERE OBSERVED. FIVE (EIGHTEEN); 3 (4); 2 (7); 1 (2); AND 5 (11) ISOLATES (NUMBERS IN BRACKETS INDICATED FIELD ISOLATES) WERE ABLE TO HEMAGGLUTINATE CATTLE, HORSE, SHEEP, CHICKEN, AND HUMAN ERYTHROCYTES, RESPECTIVELY. THE DISTRIBUTION OF HEMAGGLUTINATION PATTERN WAS DISCISSED IN THIS PAPER. Utama, Iwan Harjono; Kendran, Anak Agung Sagung; Wibawan, I Wayan Teguh; Pasaribu, Fachriyan Hasmi
Media Veteriner Vol. 7 No. 2 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

Hemagglutination activities and phenotypic expressions of fifty-five S. agalactiae isolates consisted of 19 standard and 36 field isolates from subclinical mastitis cattle were observed. Five (eighteen); 3 (4); 2 (7); 1 (2); and 5 (11) isolates (numbers in brackets indicated field isolates) were able to hemagglutinate cattle, horse, sheep, chicken, and human erythrocytes, respectively. The distribution of hemagglutination pattern was discissed in this paper.
PENGGUNAAN IMMUNOSTIK SEBAGAI UJI CEPAT SEROLOGIK UNTUK MELACAK BRUCELLA ABORTUS PADA SAPI Astarina, Dhevie Kenny; Pribadi, Eko Sugeng; Pasaribu, Fachriyan Hasmi
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.2.169

Abstract

Serological test is one of diagnostic method to detect brucellosis pathogens Several methods are being improving such as RBT, CFT and ELISA. Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to estimate sensitivity and specificity of immunostick that were used to detecte antibody brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared and ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with sensitivity 100%. Immunostick specifity were 45,45% for B. abortus S99(1) antigen; 78,79% for B. abortus S99(2) antigen and 51,52% for B. abortus S19 antigen. When the test compared with ELISA, the sensitivity 82,86% and the spesifity were 52,31% for B. abortus S99(1) antigen; 93,54% and 79,71 for B. abortus S99(2) antigen and 82,86%  and 58,46% for B. abortus S19 antigen. Keywords : immunostick assay, Brucella abortus, cattle, serologic test
PENGGUNAAN KULTUR MAKROFAG UNTUK PENGUJIAN VIRULENSI STREPTOCOCCUS EQUI SUBS. ZOOEPIDEMICUS (THE USE OF MACROPHAGE CULTURE IN VIRULENCE ASSAY OF STREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS) Utama, Iwan Harjono; Pasaribu, Fachriyan Hasmi; Wibawan, I Wayan Teguh; Setiawan, Endhie D.
Jurnal Veteriner Vol 2 No 2 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penggunaan Kultur Makrofag untuk Pengujian Virulensi Streptococcus equi subs. Zooepidemicus (THE USE OF MACROPHAGE CULTURE IN VIRULENCE ASSAY OF STREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS)
CONSTRUCTION OF A DNA VACCINE USING GLYCOPROTEIN GENE AND ITS EXPRESSION TOWARDS INCREASING SURVIVAL RATE OF KHV-INFECTED COMMON CARP (CYPRINUS CARPIO) Nuryati, Sri; Alimuddin, Alimuddin; Sukenda, Sukenda; Soejoedono, Retno Damayanti; Santika, Ayi; Pasaribu, Fachriyan Hasmi; Sumantadinata, Komar
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.942 KB) | DOI: 10.31258/jnat.13.1.47-52

Abstract

Deoxyribonucleic acid (DNA) vaccine has recently been developed as an alternative vaccine against virus infection.This study was the first step of DNA vaccine development to protect cyprinids including common carp (Cyprinuscarpio) and fancy koi (Cyprinus carpio) from KHV (koi herpesvirus) infection in Indonesia. One of KHV glycoproteingenes, i.e. glycoprotein (GP) was ligated with Japanese medaka (Oryzias latipes) รข-actin promoter to generatepAct/GP as a DNA vaccine. Fourty fish in body weight of 10-15 g/fish were individually injected by pAct/GP intomuscle in different dosage of 2.5 ?g, 7.5 ?g and 12.5 ?g/100 ?l phosphate buffer saline. Total RNA was extractedfrom the 12.5 ?g of pAct/GP-injected fish muscle at 24, 48 and 67 hours post-injection to analyze GP expression byRT-PCR method. Potential of pAct/GP as DNA vaccine was examined by injecting KHV into the 30-days-vaccinatedfish. Both of possitive and negative control fish group were not vaccinated. Possitive control fish group wereinjected with KHV, but negative control fish group were not. KHV-challenged fish were reared for 1 month, and thedeath fish were calculated daily. Result of RT-PCR analysis showed that GP gene expression were detected at 3 dpost-injection. Expression of GP in the vaccinated fish groups helped to improve their survival rate after challengedby KHV. All of fish without DNA vaccination had dead 17 days after KHV injection. The results demonstrated thatpAct/GP had high potency to be used as a DNA vaccine against KHV infection in cyprinids.
ISOLASI DAN IDENTIFIKASI BAKTERI LISTERIA MONOCYTOGENES DARI SUSU SAPI SEGAR DI KABUPATEN ENREKANG SULAWESI SELATAN Prahesti, Kusumandari Indah; Mayasari, Ni Luh Putu Ika; Malaka, Ratmawati; Yuliati, Farida Nur; Pasaribu, Fachriyan Hasmi
Acta VETERINARIA Indonesiana Vol. 5 No. 2 (2017): Juli 2017
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.097 KB) | DOI: 10.29244/avi.5.2.57-65

Abstract

Listeria monocytogenes merupakan bakteri patogen yang dapat menginfeksi manusia melalui bahan pangan sehingga menimbulkan penyakit listeriosis. Wabah listeriosis terjadi akibat konsumsi bahan pangan yang terkontaminasi L. monocytogenes, di antaranya daging, susu, dan produk susu. Serotipe bakteri L. monocytogenes dikaitkan dengan kasus wabah epidemik dan sporadik listeriosis pada manusia. Penelitian ini bertujuan untuk mengisolasi L. monocytogenes dari susu sapi segar di Kabupaten Enrekang Sulawesi Selatan, melakukan analisis karakteristik molekuler, dan menentukan serotipe isolat bakteri L. monocytogenes yang diperoleh. Sebanyak 107 sampel susu diperoleh dari lima kecamatan di Kabupaten Enrekang dan dikumpulkan menjadi 31 sampel pool, kemudian dilakukan isolasi dan identifikasi bakteri. Tahap pengayaan dilakukan dengan media Listeria enrichment broth (LEB) kemudian dilakukan kultur pada media Listeria selective agar base (LSA), dilanjutkan dengan uji biokimiawi. Isolat bakteri L. monocytogenes yang diperoleh dikonfirmasi dengan polymerase chain reaction (PCR) dan dilakukan pengurutan oligonukleotida. Identifikasi serotipe dilakukan dengan PCR multipleks. Hasil menunjukkan bahwa sebanyak 21 isolat merupakan bakteri L. monocytogenes dan analisis pengurutan oligonukleotida menunjukkan bahwa isolat yang diperoleh memiliki kemiripan sebesar 99% dengan strain L. monocytogenes yang terdapat pada basis data GenBank. Identifikasi serotipe menunjukkan bahwa keseluruhan isolat termasuk dalam serogrup 2, yaitu serotipe 1/2c dan 3c.
MP-7 ISOLATION OF ANTIBIOTIC RESISTANCE GENE FROM STAPHYLOCOCCUS AUREUS FROM CHICKEN POULTRY FARM IN BOGOR WEST JAVA INDONESIA Hermana, Nabila Swarna Puspa; Indrawati, Agustin; Pasaribu, Fachriyan Hasmi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Staphylococcus aureus is an opportunistic pathogen in human and another animal species. It also associated with staphylococcosis, osteomyelitis, arthritis, and bumble foot disease in poultry. Antibiotics including penicillin, erythromycin and tetracycline are widely used for treating infection caused by S. aureus.Chicken poultry play important role in food supply as one of protein source in Indonesia. West Java province has largest population of chicken poultry, where Bogor has the largest population of chicken poultry with 19.75.850 chicken in 2018. In order to maintain the production, farmers used antibiotics for prophylaxis, growth promotor, and therapy. However, extensive use of antibiotic can be found in low income and developed country, caused the increasing number of antibiotic resistant bacteria.The aim of this study is to determine the antibiotic resistance pattern of S. aureus isolated from cloacal swab of chicken poultry in Bogor West Java Indonesia.
Aktivitas antibakteri isolat bakteri endofit dari tanaman miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli Kusumawati, Dwi Endah; Pasaribu, Fachriyan Hasmi; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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Abstract

Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobialcompounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass,therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolateand test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. againsttwo pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophyticbacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolatesof endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus,whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.
Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri Purwanto, Ukhradiya MS; Pasaribu, Fachriyan Hasmi; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causingdisruption or damage to the host. Some studies suggest that certain endophytic bacteria can produce chemicalcompounds that have an effect on health, especially endophytic bacteria isolated from medicinal plants. Greenbetel (Piper betle L.) is a medicinal plant that has been used for years and has many benefits. The purposesof this study are to isolating and screening of endophytic bacteria from green betel against four pathogenicbacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella enteritidis). The numberof endophytic bacteria that has been isolated are 14 isolates. Based on the screening results, three isolatesof endophytic bacteria have potential activity (characterized by the formation of inhibition zone) against S.aureus. The inhibition zone may indicate that those isolates produce compounds that have antibacterial effects. Those isolates are AS1 , BS1 and BS2. The biggest inhibition zone showed by BS1, so it can be concluded thatBS1 is the most potential isolate as a novel source of antibacterial compound.