Rosmawaty Peranginangin
Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Pusat Riset Pengolahan Produk dan Sosial Ekonomi Kelautan dan Perikanan

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Purification and Characterization of Fucoidan from the Brown Seaweed Sargassum binderi Sonder sinurat, ellya; Peranginangin, Rosmawaty; Saepudin, Endang
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): August 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i2.133

Abstract

The brown seaweed Sargassumsp. is well known as a source of fucoidan. Brown seaweeds found in Indonesia seas are dominated by Sargassum sp.The objectives of this research was to purify and characterize fucoidan from S. binderi Sonder. The fresh raw material was defatted by macerated in methanol:chloroform:water (4:2:1), filtered, rinsed with acetone and then air-dried in room temperature. The defatted dried seaweed was extracted with 0.01M HCl pH 4 at room temperature. Purification has been conducted using anion exchanger. The quality of fucoidan sample was determined for total sugar, functional group using FTIR, monomer content, total ash, and total sulfate in the ester form. The result shows that pure fucoidan contains fucose as the primary sugar component, and otherminor sugars (galactose, glucose, mannose and xylose). Chemical composition of crude fucoidan consisted of 74.25% fucose; 0.28% uronate acid; 10.29% sulfate and 5.5% protein. Purification using DEAE Sephadex A-25 gave 4 fraction pools yielding total sugar (%) of F1 (17.59); F2 (18.92); F3 (13.72); F4 (49.76), respectively. The components that build the fucoidan of S. binderi Sonder were estimated derived to be from fucoidan oligomers including (1,4)-L-FucS-Gal and D-(1,4)-Gal-GalS. 
Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate Subaryono, Subaryono; Ardilasari, Yuwanita; Peranginangin, Rosmawaty; Zakaria, Fransisca Rungkat; Suhartono, Maggy Thenawidjaja
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.250

Abstract

Screening of alginate lyase producing bacteria associated with seaweed Sargassum crassifolium was carried out, and isolate S245, identified as Bacillus megaterium S245 was found to produce high alginate lyase activity. This research was conducted to evaluate activity of the alginate lyase enzyme at various pHs, temperatures and substrates. Polymannuronate and polyguluronate were used to evaluate substrate specificities. Alginate lyase activity was assayed by analysis of reducing sugar released using the 3,5 dinitrosalicylic acid (DNS) method. The research showed that the activity of alginate lyase was optimum at pH of 7.0 and  temperature of 45 0C. This enzyme was active for both polymannuronate and polyguluronate susbtrates. The Vmax and Km of this enzyme for polymannuronate and polyguluronate were 200 unit/ml/min and 79.8 mg/ml for polymannuronate substrate and 27.78 unit/ml/min and 13.17 mg/ml for polyguluronate substrate. This enzyme showed unique characteristic in working toward the two substrates.
Alginate Lyases: Sources, Mechanism of Activity and Potencial Application Subaryono, Subaryono; Peranginangin, Rosmawaty; Suhartono, Maggy Thenawidjaja; Zakaria, Fransiska Rungkat
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i3.39

Abstract

Alginate lyases are group of enzymes which catalyze depolymerization of alginate into oligosaccharides. Alginate lyase have been widely used in many applications such as in production of bioactive oligosaccharides, control of polysaccharide rheological properties, and polysaccharide structure analysis. The products of alginate lyase, polysaccharide structure analysis, alginate oligosaccharides (AOS) have many biological activities including act as prebiotics, immune modulator, anticoagulation, antioxidant, anticancer, growth promoting activities, promote production of antibiotics and ethanol. In relation to the importance of alginate lyases, their potential aplications and prospect in development of new bioactive products, we present review of the enzymes, sources, mechanism of activity and potential applications. This paper also discussed some new biological engineering in alginate lyase production.
Kandungan Logam Berat pada Kerang Kepah (Meritrix meritrix) dan Air Laut di Perairan Banjarmasin Murtini, Jovita Tri; Peranginangin, Rosmawaty
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.138

Abstract

Studies on heavy metal content of Meritrix meritrix and surrounding waters as well as the quality of Banjarmasin waters were done. Observation was carried out in June, August and October 2003 at 6 sampling points, 3 stations were 1 mile while 3 others were 2 miles from coastal line. Distance between stations was approximately 1 mile. The samples collected from each station were hard clam (Meritrix meritrix), sea water and sediment. Heavy metals content, Hg, As, Cd, Cu and Pb, in hard clam and mercury content in sea water and sediment were analyzed by atomic absorption spectrophotometer (AAS). The result showed that Hg content of Banjarmasin waters in June, August and October 2003 were 6.05, 3.17, and 4.91 ppb, respectively, indicated that Banjarmasin waters had been polluted by mercury. Whereas the hard clam in Banjarmasin waters contained Hg (1.91 ppb), As (0.88 ppb), Cd (0.22 ppb), Cu (0.46 ppb), and Pb (0.32 ppb) which were still under the maximum concentration for consumable clam.
PERBANDINGAN KEMAMPUAN DIAGNOSA ELISA DAN HPLC UNTUK DETEKSI TOKSIN DIARRHETIC SHELLFISH POISONING PADA KEKERANGAN Lusiastuti, Angela Mariana; Peranginangin, Rosmawaty
Media Akuakultur Vol 3, No 1 (2008): (Juni 2008)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.908 KB) | DOI: 10.15578/ma.3.1.2008.45-48

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui kemampuan diagnosa dari ELISA dan HPLC dalam mendeteksi toksin Diarrhetic Shellfish Poisoning pada kekerangan. Asam okadaik dideteksi menggunakan DSP Quick Check Test Kit ELISA sedangkan HPLC menggunakan 2 metode yang berbeda pada proses ekstraksi dan kondisi HPLC yang digunakan. ELISA yang digunakan adalah ELISA kompetitif menggunakan mikroplat 96 lubang, 8 x 12 strip yang dilapisi dengan antibodi monoklonal dan antiserum antimouse murni dengan konjugat peroksidase untuk mendeteksi antibodi. Metode HPLC yang digunakan meliputi metode ekstraksi sampel, proses clean up dan derivatisasi sebelum masuk ke injektor sampel otomatis. Dari hasil penelitian ini diperoleh kesimpulan bahwa ELISA dapat sebagai pilihan untuk monitoring toksin (uji tapis) sedangkan HPLC dapat digunakan untuk mengkonfirmasi sampel positif dalam membantu penegakan diagnosa. HPLC membutuhkan proses clean up dan derivatisasi supaya dapat memberikan hasil yang terbaik.
OBSERVASI LINGKUNGAN PERAIRAN DAN BIOTA PENGHASIL BIOTOKSIN DI MUARA SUNGAI MANGGAR BESAR KALIMANTAN TIMUR Sari, Abdul; Murtini, Jovita Tri; Peranginangin, Rosmawaty
Jurnal Penelitian Perikanan Indonesia Vol 10, No 3 (2004): (Vol. 10 No. 3 2004)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4285.742 KB) | DOI: 10.15578/jppi.10.3.2004.57-66

Abstract

Observasi biota penghasil biotoksin serta aspek lingkungan di perairan Balikpapan di depan muara sungai Manggar Besar, Kalimantan Timur telah dilakukan.
PENELITIAN MENGENAI KEBERADAAN BIOTOKSIN PADA BIOTA DAN LINGKUNGAN PERAIRAN TELUK JAKARTA Mulyasari, Mulyasari; Peranginangin, Rosmawaty; Suryaningrum,, Th. Dwi; Sari, Abdul
Jurnal Penelitian Perikanan Indonesia Vol 9, No 5 (2003): Vol. 9 No. 5 2003)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6705.815 KB) | DOI: 10.15578/jppi.9.5.2003.39-64

Abstract

Penelitian dilakukan di perairan pantai Teluk Jakarta yaitu sekitar Muara Angke, Muara Dadap, Cilincing dan Tanjung Pasir.
SELEKSI JENIS ANTIOKSIDAN DAN PENENTUAN KONSENTRASI OPTIMUMNYA PADA PEMURNIAN MINYAK IKAN LEMURU Irianto, Hari Eko; Fawzya, Yusro Nuri; Peranginangin, Rosmawaty
Jurnal Penelitian Perikanan Indonesia Vol 1, No 2 (1995): (Vol.1 No.2 1995)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1537.283 KB) | DOI: 10.15578/jppi.1.2.1995.1-12

Abstract

Minyak ikan mengandung asam lemak tak jenuh cukup tinggi, sehingga menyebabkan mudah rusak akibat oksidasi. Penelitian ini ditujukan untuk mendapatkan metoda peningkatan stabititas minyak ikan selama permunian dengan menggunakan antioksidan. Jenis antioksidan yang diteliti efektivitasnya pada penelitian pendahuluanadalah BHA, BHT, propil galat, TBHQ dan tokoferor.
Studi Teknik Pengeringan Gelatin Ikan dengan Alat Pengering Kabinet harianto, Harianto; tazwir, Tazwir; Peranginangin, Rosmawaty
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 3, No 1 (2008): Juni 2008
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v3i1.13

Abstract

Pengeringan gelatin dari bahan baku kulit ikan telah dilakukan menggunakan alat pengering tipe kabinet (cabinet dryer) dengan pemanasan bertahap dan aliran udara yang telah didehumidifikasi. Bahan yang akan dikeringkan adalah larutan gelatin kental yang didinginkan kemudian diekstrusi sehingga terbentuk mie gelatin kemudian diletakkan di atas tray dan dimasukkan ke ruang pengering. Untuk menghindari resiko pelumeran (melting), mie gelatin diangin-anginkan selama 2 jam pada suhu 25oC dengan udara yang didehumidifikasi. Selanjutnya suhu ditingkatkan secara bertahap dengan tingkat kenaikan 2 – 4oC perjam hingga tercapai kadar air sekitar 10%. Performansi model alat pengering tipe kabinet atau cabinet dryer yang digunakan adalah dengan spesifikasi panjang kabin 190 cm, lebar 65, dan tinggi 97 cm. Dehumidifikasi udara pengering dengan AC 0,5 HP, sumber pemanas dari lampu infra merah 3 x 1.500 Watt dengan rata-rata kecepatan aliran udara 1,1 m/detik. Hasil penelitian menunjukkan bahwa dari 2.100 g mie gelatin dengan kadar air awal 75% dihasilkan 584 g gelatin kering dengan kadar air 10%. Dibandingkan dengan alat pengering gelatin komersial maka terlihat bahwa kemampuan dehumidifikasi dari alat pengering ini perlu diperbesar untuk dapatmempersingkat waktu pengeringan.
Ekstraksi Gelatin dari Kulit Ikan Patin (Pangasius Hypopthalmus) secara Proses Asam. Peranginangin, Rosmawaty; Haq, Nurul; Rusli, Arham
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 3 (2004): JPPI ed pasca panen
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i3.376

Abstract

Ekstraksi gelatin dari kulit ikan patin (Pangasius hypopthalmus) telah dilakukan dengan perlakuan perendaman kulit dalam asam sitrat dalam berbagai pH yaitu.‑ 2, 3 dan 4 dengan variasi lama perendaman 12, 24 dan 36 jam. Kulit kemudian dicuci sampai pH netral dan gelatin diekstraksi pada suhu 70 dan 90 derajad Celcius selama 2 jam. Filtrat dikeringkan dalam oven suhu 50 derajad Celcius selama 36‑48 jam sehingga diperoleh lembaran gelatin. Penentuan perlakuan yang terbaik untuk ekstraksi gelatin dipilih berdasarkan rendemen, viskositas, kekuatan gel dan pH gelatin. Hasil penelitian menunjukkan bahwa perlakuan perendaman dalam asam sitrat pH 3 selama 12 jam, suhu ekstraksi 90 derajad Celcius selama 2 jam memberikan hasil yang terbaik yang diperlihatkan dari rendemen 9,36%, viskositas 10,1 cps, kekuatan gel 202,55 bloom dan pH 6,7.