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APLIKASI SISTEM RESIRKULASI PADA PENDEDERAN IKAN KAKAP PUTIH, LATES CALCARIFER KEPADATAN TINGGI Permana, Gusti Ngurah; Pujiastuti, Zeny; Fakhrudin, Fakhrudin; Muzaki, Ahmad; Mahardika, Ketut; Adiyana, Kukuh
Jurnal Riset Akuakultur Vol 14, No 3 (2019): (September, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.14.3.2019.173-182

Abstract

 Teknologi resirkulasi (Recirculating aquaculture system [RAS]) dikembangkan untuk meningkatkan produktivitas benih ikan kakap putih. Tujuan dari penelitian ini untuk mengetahui pengaruh perbedaan kepadatan pada pendederan benih ikan kakap dengan sistem RAS. Penelitian ini menggunakan sistem RAS dengan 12 bak dengan volume 1,0 m3 yang terbagi dalam dua modul dengan masing-masing modul terdiri atas enam bak. Untuk perbandingan digunakan sistem sirkulasi yang mengadopsi teknologi yang ada di masyarakat menggunakan tiga bak beton dengan volume 1 m3. Benih ikan kakap putih yang dipergunakan memiliki panjang rata-rata: 2,87 cm ± 0,18 cm dan bobot rata-rata: 0,39 ± 0,07 g. Perlakuan menggunakan perbedaan kepadatan yaitu (A) 3.000 ekor/m3 (1,17 kg/m3); (B) 4.500 ekor/m3 (1,75 kg/m3); dan (C) sirkulasi 1.500 ekor/m3 (0,62 kg/m3). Parameter yang diamati meliputi: pertumbuhan, sintasan, kualitas air, dan pada akhir penelitian, sampel darah diambil untuk menentukan kesehatan ikan yang berhubungan dengan parameter haematokrit darah. Analisis data secara deskriptif dan uji t test. Hasil penelitian menunjukkan bahwa perbedaan padat penebaran pada sistem RAS dan sirkulasi secara signifikan tidak menunjukkan perbedaan (P>0,05) pertumbuhan panjang dan bobot benih. Sintasan tertinggi diperoleh pada kelompok ikan kontrol, diikuti dengan kelompok ikan dengan kepadatan 3.000 ekor/m3 dan terendah pada kelompok ikan dengan kepadatan 4.,500/m3 ekor. Nilai hematokrit dalam darah lebih tinggi (P<0,05) ditunjukkan dari kelompok ikan dengan kepadatan yang lebih rendah. Hasil tersebut menunjukkan bahwa aplikasi sistem resirkulasi pada pendederan ikan kakap putih dapat dilakukan dengan penerapan kepadatan 3.000 ekor/m3 (31,04 kg/m3).Recirculating aquaculture system (RAS) has been developed to increase the productivity of barramundi nursery. This study used 12 tanks with a volume of 1.0 m3 each. The tanks were grouped into two modules, each module consisted of six tanks. As a comparison, a circulation system adopted by the local community was used which consisted of three concrete tanks with a volume of 1 m3. Barramundi juveniles with an average length: 2.87 cm ± 0.18 cm and an average weight of 0.39 ± 0.07 g) were used in the experiment. The treatments were differences in stocking densities: 3,000 fish/m3 (1.17 kg/m3); 4,500 fish/m3 (1.75 kg/m3); and control 1,500 fish/m3 (0.62 kg/m3). Data collected included growth of survival and water quality variables (temperature, salinity, DO, pH, nitrite, NH3, total bacteria/vibrio) and blood hematocrit. The results of this study showed that fish densities (4,500; 3,000; and 1,500 fish/m3) did not affect fish growth. However, the survival rate was significantly different (P<0.05) among the treatments. The values of hematocrit were significantly (P<0.05) higher at the density of 46.56 kg/m3. These results suggest that the RAS application can sustain a nursery density of Barramundi up to 3,000 ind./m3 (31.04 kg/m3).
Biochemical Genetic Analysis Of Three Population Of Marble Grouper, Epinephelus polypekhadion Permana, Gusti Ngurah; Haryanti, Haryanti; Moria, Sari Budi; Giri, Nyoman Adiasmara; Sugama, Ketut
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.32

Abstract

Genetic variations of marble groupers, Epinephelus polypekhadion were evaluated to know genetic performance of fish to support fish seed production. Fifty fish samples from three locations i.e. West Nusa Tenggara, South Sulawesi and East Java were collected for analysis. Genetic analysis has been examined through allozyme electrophoresis by using 11 enzymes (ADH, GPI, SDH, IDH, LDH, ME, PGM, 6PGD, MPI, SP, EST). The result showed that 16 loci were detected, and three of them were polymorphism enzymes namely Isocitric dehydrogenase (IDH*), Glucose Phosphate Isomerase (GPI-1*) and Esterase (EST-2*). One locus (EST-2) was polymorphic in the East Java and West Nusa Tenggara populations and three loci (EST-2, GPI-1 and IDH) were polymorphic in the South Sulawesi population. The heterozygosity ranged from 0.007 to 0.034. Rogers genetic distance between population pairs were ranged from 0.005 to 0.012 (average = 0.009). Differences between genetic populations were significant (P<0.05). East Java and West Nusa Tenggara populations have same gene pool.
Polimorfisme Enzim Glucose-6-Phosphate Isomerase pada Tiga Populasi Tuna Sirip Kuning (Thunnus albacares) Permana, Gusti Ngurah; Hutapea, Jhon H.; Moria, Sari Budi; Haryanti, Haryanti
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.163

Abstract

Samples of yellowfin tuna (Thunnus albacares) were taken from three locations Bali, North Sulawesi and North Maluku. The glucose-6-phosphate isomerase (GPI) was analyzed from liver using allozyme electrophoresis method. Polymorphism of GPI enzyme was observed and four alleles (A, B ,C, D) were found in Bali population, three alleles (A,B,C) were found in North Maluku and North Sulawesi populations. Heterozygosity values, from Bali, North Maluku and North Sulawesi were 0.419; 0.417; 0.143 respectively. Genetic distance between North Sulawesi and North Maluku were 0.029, and with Bali population was 0.353. These results indicated that North Maluku and North Sulawesi population were not separate by geographic barrier, therefore genetic distance of both populations was closed. However, those populations were genetically separated to the Bali population might be due to major hydrological barrier.
ASPECTS OF LEOPARD CORAL GROUPER (Plectropomus leopardus) REPRODUCTION IN INDONESIA Andamari, Retno; Sembiring, Sari Budi Moria; Permana, Gusti Ngurah
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.853 KB) | DOI: 10.15578/iaj.2.1.2007.51-57

Abstract

Leopard coral grouper, Plectropomus leopardus is one of the most economically important finfish fish in Indonesia and the demand for the grouper is rapidly increasing in Asia and the Pacific. Grouper exports from Bali were 1,613 mt in 2001, 2,082 mt in 2002 and 2,861 mt in 2003. Understanding the reproductive biology of fishes is an important component in developing mariculture and in the management of capture fisheries. This study reports on the reproductive biology of 86 coral groupers collected from various locations in Indonesia. The length and weight of these fish were recorded and related to gonad development. There was a strong relationship between length and weight; weight being proportional to the length raised to the power (b value) 3.2. As the value of b was greater than 3, this suggests that growth is allometric. Histological analysis 73% of the fish were immature, 19% were in transition from females to males, only 4% were male, and only 2 fish (2%) had mature gonads: these were female. The sex of 2 fish could not be determined. From these data it can be seen that the leopard coralgrouper has asynchronous gonad development. The two fish that were mature contained 343,980 and 429,259 oocytes and three distinct sizes of oocytes could be found. This suggests that the grouper is a multiple spawner. If fish are required for brood stock, this study has shown that only those with a length greater than 35 cm in standard length should be taken from the wild.
GENETIC IDENTIFICATION FOR TUNA AND RAINBOW RUNNER CAPTURE IN NORTH BALI WATERS Permana, Gusti Ngurah; Hutapea, Jhon Harianto; Haryanti, Haryanti; Sembiring, Sari Budi Moria; Nakazawa, Akio
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.62 KB) | DOI: 10.15578/iaj.1.1.2006.29-34

Abstract

Gondol Research Institute for Mariculture identification of tuna and rainbow runner was an objective in this current study. Samples of five species were collected from territorial water of North Bali. The method used in this study was allozyme electrophoresis. The results showed that buffer of CAPM-6 (citric acid aminoprophylmorpholine) resulted in a sharp and clear banding pattern. The species could be differentiated in six diagnostic isozyme patterns Idh* (isocitrate dehydrogenase), 6Pgd* (6 phosphogluconate dehydrogenase), Gpi* (glucose phosphate isomerase), Mdh* (malate dehydrogenase), Est* (esterase), and Sp* (sarcoplasmic protein). All species were in Hardy-weinberg equilibrium. Heterozygosities of species were ranged from 0.00 to 0.099. Yellowfin tuna has the highest heterozigosity compared with the other species. Clustering samples according to pairs revealed that genetic distance of Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) had small value (0.001). By contrast, the largest value was observed between yellowfin tuna, T. albacares and rainbow runner, E. bipunnulata (0.007). This value indicated that Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) closed relation, while among yellowfin tuna, skipjack tuna, and rainbow runner, were separated phylogenically.
KAJIAN BIOREPRODUKSI DAN KOMPOSISI PROKSIMAT DAGING IKAN TUNA SIRIP KUNING (Thunnus albacares) DARI BEBERAPA PERAIRAN DI INDONESIA Hutapea, Jhon Harianto; Andamari, Retno; Giri, I Nyoman Adiasmara; Permana, Gusti Ngurah
Jurnal Riset Akuakultur Vol 1, No 3 (2006): (Desember 2006)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1562.994 KB) | DOI: 10.15578/jra.1.3.2006.325-336

Abstract

KAJIAN BIOREPRODUKSI DAN KOMPOSISI PROKSIMAT DAGING IKAN TUNA SIRIP KUNING (Thunnus albacares) DARI BEBERAPA PERAIRAN DI INDONESIA
PEMELIHARAAN INDUK IKAN TUNA SIRIP KUNING, Thunnus albacares DALAM BAK TERKONTROL Hutapea, Jhon Harianto; Permana, Gusti Ngurah; Setiadi, Ananto
Jurnal Riset Akuakultur Vol 5, No 1 (2010): (April 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1430.663 KB) | DOI: 10.15578/jra.5.1.2010.79-90

Abstract

Intensifikasi penangkapan ikan tuna baik yang langsung dipasarkan maupun dibesarkan dalam usaha budidaya, berpengaruh negatif terhadap kelestarian populasi ikan ini di alam. Dengan demikian upaya perbenihan secara buatan perlu dilakukan untuk mengurangi tekanan terhadap populasi alam. Balai Besar Riset Perikanan Budidaya Laut, Gondol telah merintis perbenihan ikan tuna sirip kuning (Thunnus albacares) sejak tahun 2003 bekerjasama dengan Overseas Fishery Cooperation Foundation (OFCF) Jepang. Induk ikan tuna telah berhasil dibesarkan dan dipijahkan dalam bak beton bervolume 1.500 m3 secara terkontrol, dengan sistem pergantian air semi tertutup. Pakan yang diberikan berupa ikan layang dan cumi-cumi sekitar 2,5% biomassa per hari. Pemijahan pertama terjadi pada bulan Oktober tahun 2004, ukuran induk diperkirakan lebih dari 9,138 kg atau panjang cagak lebih dari 82 cm dengan perkiraan umur sekitar 2 tahun. Puncak pemijahan terjadi pada tahun 2005 dan 2006 dengan frekuensi pemijahan masing-masing lebih dari 100 kali. Pemeliharaan induk ikan tuna dengan kepadatan 0,66 kg/m3 belum dapat dikatakan sebagai kepadatan maksimum dan peningkatan kematian cenderung akibat ruang gerak yang semakin sempit seiring dengan pertumbuhan induk. Namun demikian kendala yang ditemukan dalam pemeliharaan induk adalah kematian akibat menabrak dinding bak sedangkan kendala dalam pemeliharaan larva adalah serangan endoparasit pada telur
STIMULASI PERTUMBUHAN JUVENIL ABALON, Haliotis squamata DENGAN PEMBERIAN HORMON REKOMBINAN IKAN rElGH Khotimah, Fitriyah Husnul; Permana, Gusti Ngurah; Rusdi, Ibnu; Susanto, Bambang; Alimuddin, Alimuddin
Jurnal Riset Akuakultur Vol 11, No 4 (2016): (Desember 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.158 KB) | DOI: 10.15578/jra.11.4.2016.331-338

Abstract

Masalah yang paling utama dalam budidaya abalon tropis adalah pertumbuhan yang lambat. Penggunaan rElGH (recombinant giant grouper, Epinephelus lanceolatus growth hormone) untuk menstimulasi pertumbuhan beberapa spesies ikan sudah dilakukan. Penelitian ini bertujuan untuk menguji akselerasi pertumbuhan juvenil abalon tropis, Haliotis squamata setelah diberi perlakuan perendaman hormon rekombinan ikan kerapu kertang, Epinephelus lanceolatus pada frekuensi yang berbeda. Ada empat perlakuan frekuensi perendaman rElGH yaitu 4, 9, 16 kali, dan tanpa perendaman (kontrol). Masing-masing perlakuan diulang tiga kali. Perendaman dilakukan selama tiga jam, dengan interval waktu empat hari. Kepadatan abalon tropis 100 ekor/L air laut yang mengandung 30 mg rElGH. Wadah untuk perendaman berupa beaker glass yang dilengkapi dengan aerasi. Penelitian dilakukan selama tujuh bulan. Hasil penelitian menunjukkan bahwa abalon tropis yang direndam rElGH dengan frekuensi empat kali menghasilkan pertumbuhan bobot tubuh dan panjang cangkang tertinggi dan berbeda nyata dengan perlakuan lainnya (P&lt;0,05). Sintasan abalon tropis yang diberi perlakuan perendaman hormon rElGH lebih tinggi dibandingkan perlakuan kontrol.The most crucial problem in tropical abalone aquaculture is the slow growth of the species. Studies investigating the use of rElGH (recombinant giant grouper, Epinephelus lanceolatus growth hormone) for promoting growth have been performed in various species. This research aimed to examine the growth acceleration of tropical abalone, Haliotis squamata juvenile after being treated in different immersion frequencies of recombinant giant grouper, Epinephelus lanceolatus growth hormone (rElGH). There were four treatments of rElGH immersion frequency: 4, 9, 16 times and without rElGH immersion (control). Each treatment was performed in triplicates. Immersion was performed for 3 hours, at 4-day intervals and a density of 100 tropical abalones in 1 L seawater containing 30 mg rElGH. Immersion was conducted in a beaker glass supplied with oxygen. The results indicated that rElGH immersion for total of 4 times showed significantly higher (P&lt;0.05) body weight and shell length of tropical abalone compared to other treatments. The Survival of tropical abalone treated with rElGH was also significantly higher than control.
PENENTUAN JENIS KELAMIN BERDASARKAN REAKSI ANTIBODI PADA IKAN TUNA Permana, Gusti Ngurah; Haryanti, Haryanti
Media Akuakultur Vol 2, No 2 (2007): (Desember 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4153.25 KB) | DOI: 10.15578/ma.2.2.2007.77-79

Abstract

Pada budi daya ikan, identifikasi individu sangat penting untuk diketahui terutama dalam hal mengidentifikasi jenis kelaminnya. Bagaimana membedakan jantan atau betina ? Secara sepintas untuk membedakan ikan jantan atau betina memang mudah tetapi pada kenyataannya tidaklah demikian. Reaksi antibodi merupakan salah satu pendekatan yang dapat dipergunakan untuk penentuan jenis kelamin pada ikan yang telah menunjukkan aktivitas hormon dan pembentukan vitelogenin. Hasil dari kajian analisis dengan metode dot blot adalah ekspresi vitelogenin nampak jelas pada individu betina dan efek plasma terlihat transparan dibandingkan pada individu jantan tetapi metode ini sangat memerlukan pengalaman dan keahlian dalam akurasi pembacaan hasil.
PEMELIHARAAN LARVA ABALON Haliotis squamata DENGAN PEMBERIAN JENIS PAKAN BERBEDA DALAM BENTUK TEPUNG Khotimah, Fitriyah Husnul; Permana, Gusti Ngurah; Rusdi, Ibnu; Susanto, Bambang
Jurnal Riset Akuakultur Vol 13, No 1 (2018): (Maret 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.108 KB) | DOI: 10.15578/jra.13.1.2018.39-46

Abstract

Masalah utama yang umum terjadi pada produksi benih abalon adalah kematian yang tinggi (&gt; 90%) setelah abalon menempel pada plate pemeliharaan. Penggunaan pakan dalam bentuk tepung untuk mengganti diatom sebagai pakan postlarva beberapa spesies ikan, udang, dan abalon sudah dilakukan. Penelitian ini bertujuan untuk mendapatkan jenis pakan dalam bentuk tepung yang sesuai dan efektif untuk mendukung sintasan dan pertumbuhan larva abalon Haliotis squamata. Percobaan terdiri atas lima perlakuan pakan pada pemeliharaan larva abalon yaitu tepung Spirulina sp., Ulva sp., Chaetoceros sp., Gracilaria sp., dan diatom (kontrol). Masing-masing perlakuan terdiri atas empat ulangan. Pakan berupa tepung yang digunakan pada masing-masing perlakuan, terlebih dahulu dicampur merata dengan larutan tepung agar (7,5 mg/mL dalam air laut; suhu 40°C) dengan konsentrasi tepung 40 mg/mL larutan agar. Pemberian pakan dilakukan setiap tiga hari dengan cara menyemprotkan larutan pakan pada permukaan plate pemeliharaan larva. Penelitian dilakukan selama 30 hari. Hasil penelitian menunjukkan bahwa sintasan larva abalon yang diberi pakan tepung Spirulina sp. paling tinggi dan berbeda nyata (P&lt;0,05) dengan yang diberi diatom, tepung Chaetoceros sp., dan Ulva sp., yaitu masing-masing 81,49%; 79,25%; 76,57%; dan 76,46%; tetapi tidak berbeda nyata dengan yang diberi pakan tepung Gracilaria sp. 81,37% (P&gt;0,05). Laju pertumbuhan harian panjang cangkang larva abalon tertinggi diperoleh pada larva yang diberi pakan tepung Gracilaria sp. (203,81 ± 1,23 µm/hari) dan Spirulina sp. (205,59 ± 1,71 µm/hari). Nilai laju pertumbuhan harian panjang cangkang larva abalon yang paling rendah dijumpai pada larva yang diberi pakan tepung Ulva sp. (146,07 ± 1,73 µm/hari).The most common problem in abalone seed production is the high mortality occurrence (&gt; 90%) after postlarvae settlement to the rearing plates. The use of microparticle diets to replace the natural feed of postlarval has been performed on various species of fish, shrimp, and abalone. This research aims to determine the most effective and suitable powder-based feed to support the survival and growth of abalone Haliotis squamata larvae. The experiments consisted of five feed treatments, i.e., Spirulina sp., Ulva sp., Chaetoceros sp., and Gracilaria sp. Flour, and diatoms (as control). Each treatment had four replicates. The powder-based feed used in each treatment was firstly mixed with a solution of agar powder (7.5 mg/mL sea water, 40°C) with a concentration of 40 mg of flour/mL of agar solution. Feeding was done every three days by spraying the feed solution onto the surface of the larval rearing plate. The study was conducted for 30 days. The results showed that survival rate of abalone larvae fed with Spirulina sp. flour was the highest and significantly different (P&lt;0.05) compared with those given diatoms, Chaetoceros sp. and Ulva sp. flours, which were 81.49%, 79.25%, 76.57%, and 76.46%, respectively, and not significantly different from those fed with Gracilaria sp. 81.37% (P&gt;0.05). The highest daily growth rate of the shell length of abalone larvae was achieved by larvae fed with Gracilaria sp. (203.81 ± 1.23 ¼m/day) and Spirulina sp. flours (205.47 ± 1.71 µm/day). The lowest daily growth rate of shell length was found on abalone larvae fed with Ulva sp. flour (146.07 ± 1.73 µm/day).