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Pektin Dalam Tepung Kesemek Mempengaruhi Kadar Trigliserida Pada Tikus Wistar Jantan Yang Diberi Diet Aterogenik Setyaningtyas, Stefania Widya; Permatasari, Nur; Mustafa, Annasari
Amerta Nutrition Vol 1, No 1 (2017): AMERTA NUTRITION
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.652 KB) | DOI: 10.20473/amnt.v1i1.2017.38-45

Abstract

Background: Moderate hypertriglyceridemia is almost certainly an independent risk factor for cardiovascular disease. Pectin is a kind of soluble fiber that can be used to decrease triglyceride level and it  can be found in fruits such as persimmon. Objective: This research intens to prove the effect of soluble fiber of persimmon flour to decrease triglyceride level. Methods: The search used Post Only Control Group design. Normal diet, atherogenic diet, and atherogenic diet with various amount of dried persimmons were given to 30 subjects, male wistar rats, for 12 weeks. 1.2 g, 2.4 g, and 3.6 g persimmon flour were used as the given amount for every groups. Results: There were significant differences between atherogenic diet group and the other groups of research (p-value<0.001). Compared to normal diet group, atherogenic diet with 1.2 g and 2.4 g persimmon flour resulted unsignificant differences of triglycerides level. But, atherogenic diet with 3,6 g dried persimmon group was different significantly to normal diet groups (p-value=0.036). Both dose of persimmon flour and fat intake affect elevation of tryglyceride level up to 62.5%. Conclusion: The contribution of persimmon flour (Diospyros Kaki L. var Junggo) in inhibitation of the increase of triglyceride serum level on male wistar rats.  The most effective dose is 1.2 g, because the result of the trigliceride level was closest to normal and energy intake was not affected. ABSTRAK Latar belakang : Hipertrigliseridemia kadar sedang hampir pasti merupakan faktor risiko tersendiri untuk penyakit kardiovaskular. Pektin merupakan jenis serat larut air memiliki efek menurunkan trigliserida dan banyak terdapat dalam buah-buahan, salah satunya buah kesemek.Tujuan : Penelitian ini bertujuan menguji efek serat larut air pada tepung kesemek dalam menurunkan kadar trigliserida.Metode : Penelitian dilakukan dengan metode Post Test Only Control Group. Diet normal, diet aterogenik, diet aterogenik + kesemek dengan berbagai jumlah diberikan kepada 30 subyek penelitian, yaitu tikus wistar jantan selama 12 minggu. 1,2 g, 2,4 g, dan 3,6 g tepung kesemek digunakan sebagai intervensi untuk kelompok perlakuan.Hasil : terdapat perbedaan kadar trigliserida yang signifikan antara kelompok diet aterogenik dengan kelompok perlakuan lainnya (p=0,000). Bila dibandingkan dengan kelompok diet normal, perlakuan dengan pemberian tepung kesemek 1,2 g dan 2,4 g menghasilkan kadar trigliserida yang tidak berbeda signifikan, namun jumlah pemberian tepung kesemek 3,6 g berbeda nyata dengan diet normal (p=0,036). Pemberian tepung kesemek dan asupan lemak bersama-sama mempengaruhi pembentukan trigliserida dengan kontribusi sebesar 62,5%.Kesimpulan : Tepung kesemek (Diospyros Kaki L. Var. Junggo) terbukti dapat menghambat peningkatan kadar trigliserida serum pada tikus wistar jantan yang diberi diet aterogenik. Jumlah yang dinilai paling efektif menurunkan trigliserida adalah sebesar 1,2 g, karena pada jumlah tersebut, kadar trigliserida yang dihasilkan paling mendekati normal dan tidak mempengaruhi asupan energi.
PENGARUH PEMBERIAN MORFIN DOSIS LETHAL TERHADAP PERTUMBUHAN LARVA LALAT Chrysomya sp. PADA MEDIA BANGKAI TIKUS Rattus norvegicus GALUR WISTAR Fadhlurrahman, Ahmad Reza; Baskoro, Aswin D; Permatasari, Nur
MANDALA of Health Vol 6, No 1 (2013): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Abstract

Death is the end of life. To identify unproper death on corpses, we use Post Mortem Interval (PMI). Insect evidence on the corpses can be used to estimate the Post Mortem Interval, however many factors including drugs may affect the rate of fly larva growth. Two rats with average BW 200 grams were used in this experimental study in order to analyze the influence of morphine on growth rate of fly larvae. One rat were given 120 grams of morphine orally (LD50 = 461mg/KgBW), and the other were killed by cervical dislocation and used as control. The rats were then simultaneously killed and caged individually into Chrysomya sp. cages to let the flies deposit their eggs on the rat corpses. Five developing larvae were sampled twice daily to determine the body length, weight and the growth rate until they were emerging to be adult. Result of this study showed that the length and the weight were lower and the growth of Chrysomya sp. larvae in rat corpses containing morphine were faster than control. The significant result occured at third stadium (p &lt; 0,005) This result suggested that morphine can precipitate the growth rate of fly larvae.
The Effectiveness Of Tofu Liquid Waste As A Natural Phytoestrogen For Mandibular Bone Of Ovariectomized Rats Aquina, Meilia; Permatasari, Nur
Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Estrogen is a steroid compound that has many benefits for the bodys physiology.Hypoestrogenic women could have periodontitis in their mouth. Hormonereplacement therapy (HRT) is a therapy that used to reduce the disturbance causedby estrogen deficiency. However, HRT has many side effects. Theoretical studyshows that isoflavones in tofu liquid waste has an element of a steroid compound.The purpose of this study was to prove the effectiveness of isoflavones in the tofuliquid waste by examining the microscopic structure, estrogen receptor expressionand MDA levels in rat mandibular bone post-ovariectomy. This research methoduse a true experimental laboratory with randomized post test control group design.Twenty-four female wistar rats were divided randomly into six groups, consistedof a group of normal rat that does’nt ovariectomized (C1), 4 weeksovariectomized rats (C2), 8 weeks ovariectomized rats (C3) but no tofu liquidwaste was given, and 8 weeks ovariectomized rats that given tofu liquid waste atthe end of the 4th week of ovariectomy with three different doses (C4 = 1.2, C5 =6; C6 = 12 ml / kg). Histopathological slide used to see the changes resulted in anumber of osteoclasts, osteocytes, periodontal ligament width, alveolar boneheight, the amount of estrogen receptor expression and MDA levels. The resultsof statistical tests show that there is a difference in the rats between six differentgroups (ANOVA, p &lt;0.05) and there is a close relationship between the dose ofthe tofu liquid waste with all parameter. The conclusion of this study is tofuliquid waste could improve the microscopic structure, estrogen receptorexpression and MDA levels in rat mandibular bone post-ovariectomy.
EffectOf Panax Ginseng Extract For The Increased Number Of Fibroblasts Cells After Tooth Extraction Rizky Hutomo, Ferdian; Permatasari, Nur; Andari Wulan, Kartika
Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

The process of tooth extraction can lead to injury. One of the factors that influencethe process of wound healing after tooth extraction is the number of fibroblastcells. The content of saponins can increase the number of fibroblasts cells aftertooth extraction. Asian ginseng plants known to contain saponins (ginsenoside).The purpose of this study was to determine the effect of Asian ginseng extract(Panax ginseng extract) for the increased number of fibroblasts cells after toothextraction. The research method used is in vivo experimental design with thedesign methods Randomized Post Test Only Control Group Design. In this studyused twentty male rattus novergicus and divided into four group, consisting ofcontrol group (K1) were not given Asian ginseng extract, treatment groupsnumber one (K2) were given 25mg/Rattus novergicus/day doses of Asianginseng, treatment groups number two (K3) were given 50mg/ Rattusnovergicus/day doses of Asian ginseng, and treatment groups number three (K4)were given 75mg/Rattus novergicus/day doses of Asian ginseng. Histopathologicpreparations used for counting the number of fibroblasts cells. The results ofstatistical tests indicate there are differences in the number of fibroblast cells inRattus novergicus between the four different groups (ANOVA, p &lt;0.05) and thereis a very strong correlation between increasing doses of ginseng extract with anumber of fibroblast cells in Rattus novergicus tooth socket (Pearson, R = 0.915 p&lt;0.05). The conclusion of this study is Asian ginseng extract can increase thenumber of fibroblasts in the tooth socket after tooth extraction, it is recommendedthat research about the amount of saponin in Asian Ginseng extract
EFEK KOMBINASI ARTEMISININ DAN N-ACETYLCYSTEINETERHADAP KADAR MALONDIALDEHYDE(MDA) OTAK DAN PARU MENCIT GALUR BALB/C YANG DIINFEKSI PLASMODIUM BERGHEI Fitri, Loeki Enggar; Iskandar, Agustin; Permatasari, Nur; Gunawan, Joko Agus; Indrawan, Khadafi
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.577 KB) | DOI: 10.21776/ub.jkb.2008.024.02.4

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Cerebral and lung damage during malaria infection is believed to be caused by free radicals activitiesthat are produced during immunology process. The free radicals react with lipid component of cellular membrane which generates malondialdehyde (MDA) as its end-product. The aim of the research was to determine whether combination of artemisinin and NAC was moreeffective in decreasing cerebral and lung MDA level compared to artemisinin mono-therapy . The researchwas post-test-control-only design using 5 groups consisted of group A (negative control group), group B mice which infected with P.berghei without therapy (positive control group), group C mice which infected with P.berghei and received artemisinin mono-therapy (0.04 mg/g BW for 7 days), group D mice which infected with P.berghei and received artemisinin in combination with NAC (1 mg/g BW for 7 days) and group E mice which infected with P.berghei and received artemisinin in combination with NAC (1 mg/g BW for 3 days and tapered into ½ mg/g BW for 4 days). On the 3rd, 5th,and 7thday, 3 mice from each group were scarified and assayed for MDA level. On the 3rd day, a decreasing trend of cerebral and lung MDA level wasobserved on all treatment groups. On the 5thday, a decreasing trend of cerebral and lung MDA level wasobserved in group that received artemisinin and NAC whereas group?s that received artemisinin mono-therapy increased. Cerebral and lung MDA level of groupthat received artemisinin mono-therapy was significantly different with group that received combination of artemisinin and NAC in constant dose (p = 0.014) and with group that received combination artemisinin  and NAC in tapering dose (p = 0.004).
PENGARUH EKSTRAK JINTAN HITAM TERHADAP MDA DAN SEL SPERMATOGONIUM TIKUS YANG DIPAPAR ASAP ROKOK KRETEK SUBAKUT P, Happy Kurnia; Permatasari, Nur; Subandi, Subandi
Jurnal Kedokteran Brawijaya Vol 26, No 3 (2011)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (868.083 KB) | DOI: 10.21776/ub.jkb.2011.026.03.6

Abstract

Radikal  bebas  dalam  asap  rokok  diketahui  dapat  menyebabkan  gangguan  infertilitas.  Aktivitas  radikal  bebas  dapat dihambat oleh antioksidan seperti yang terkandung dalam jintan hitam (thymoquinone, nigellone, carvacrol, t-anetholedan 4-terpineol). T ujuan penelitian ini  adalah untuk mengetahui pengaruh ekstrak jintan hitam terhadap kadar Malon Di Aldehid  (MDA)  testis  dan  jumlah  sel  spermatogonium  testis  tikus  yang  dipapar  asap  rokok  kretek  selama  21  hari  (subakut). Penelitian eksperimental ini menggunakan 20 ekor tikus (Rattus norvegicus) strain Wistar jantan yang dibagi menjadi 5 kelompok,  terdiri  dari  kelompok  kontrol  negatif,  kelompok  kontrol  positif ,  kelompok  perlakuan  1,  2,  dan  3  (masing-masing dipapar asap rokok kretek dan diberi ekstrak jintan hitam dosis 0,6; 1,2; 2,4 g/kgBB/hari secara berurutan). Kadar MDA testis  diukur  menggunakan  metode  Thio  Barbituric  Acid  test  (TBA  T est).  T estis  tikus  dijadikan  preparat  histopatologi menggunakan  pengecatan  Haematoxcylin  Eosin  (HE),  untuk  dihitung  jumlah  sel  spermatogonium  yang  melekat  pada membrana  basalis.  Dapat  disimpulkan  bahwa  jintan  hitam  mempunyai  efek  antioksidan,  yaitu  dapat  mencegah penurunan jumlah  sel spermatogonium pada tubulus  seminiferus testis  tikus strain Wistar melalui hambatannya pada pembentukan  peroksidasi  lipid  (MDA)  akibat  paparan  asap  rokok  kretek  subakut.
MEKANISME KERJA BENALU TEH PADA PEMBULUH DARAH AS, Nour Athiroh; Permatasari, Nur
Jurnal Kedokteran Brawijaya Vol 27, No 1 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (834.06 KB) | DOI: 10.21776/ub.jkb.2012.027.01.1

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Secara garis besar ada dua jenis  penatalaksanaan  terhadap hipertensi yaitu menggunakan obat-obatan dan non obat. Adapun penatalaksanaan secara non obat, salah satunya dengan terapi   herbal dengan memanfaatkan benalu teh dari famili  Loranthaceae  misalnya  (Viscum  album,  Dendrophtoe  pentandra  (L.)  Miq, Scurrula  parasitica,  Scurulla  oortiana,  dan Macrosolen javanus)  yang  berpotensi  sebagai  antihipertensi  (vasodilator). Mekanisme  kerja  dari flavonoid  benalu teh sebagai vasodilator karena peran otot polos dan endotel pembuluh darah. Pada umumnya   pengobatan hipertensi yaitu pada    organ  target  pembuluh  darah  (sistem  vaskular).  Flavonoid  benalu  teh  dalam  hal  ini  quercetin  mampu  bekerja langsung pada otot polos pembuluh arteri dengan menstimulir atau mengaktivasi Endothelium Derived Relaxing Factor(EDRF) sehingga menyebabkan vasodilatasi. Beberapa penelitian tentang pengaruh flavonoid tanaman teh pada fungsi endotel bahwa kandungan dari flavonoid yaitu polifenol dapat meningkatkan aktivitas dari Nitric Oxide Synthase (NOS) pada  sel  endotel  pembuluh  darah.  Quercetin  mempunyai  potensi  meningkatkan  produksi  Nitric Oxide  (NO)  di  sel  endotel. Zat  aktif  tersebut  mampu  mensintesa  NO  dalam  endotel  dan  berdifusi  secara  langsung  ke  otot  polos  selanjutnya merangsang guanylate cyclase untuk membentuk cGMP sehingga terjadi vasodilatasi.
POTENSI KARBOKSIMETIL KITOSAN SEBAGAI ANTIKANKER DENGAN PENDEKATAN LABORATORIUM Ibrahim, Moh. Nuh; Widjajanto, Edi; Permatasari, Nur; Sabarudin, Akhmad
Jurnal Fish Protech Vol 1, No 1 (2018): Jurnal Fish Protech, April 2018
Publisher : Universitas Halu Oleo

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Abstract

Faktor prilaku dan pola makan yang tidak sehat dapat menyebabkan penyakit kanker. Penyakit ini menjadi penyebab utama kematian seluruh dunia.  Kanker dapat terjadi akibat paparan radikal bebas atau disebut ROS salah satu produknya adalah hydrogen peroksida. Ketidakseimbangan ROS dengan antioksidan menyebabkan oxidative stress dan oxidative damage sel tubuh. Untuk itu dibutuhkan antioksidan pencegah terjadinya kanker. Karboksimetil Kitosan dapat menurunkan kadar hydrogen peroksida pada tikus uji setelah dipapar dengan allergen,  melalui gugus aktifnya. Efektivitas Karboksimetil Kitosan ini sangat berpotensi sebagai antikanker.
VITAMIN E MEMPERTAHANKAN KEMAMPUAN EPC YANG DIPAPAR GLUKOSA TINGGI DALAM PELEPASAN NO DAN INDUKSI MIGRASI SEL ENDOTEL Nugrahenny, Dian; Widodo, M Aris; Permatasari, Nur
Jurnal Kedokteran Brawijaya Vol 27, No 1 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1004.234 KB) | DOI: 10.21776/ub.jkb.2012.027.01.2

Abstract

Peran sel progenitor endotel (EPC) dalam angiogenesis terganggu pada diabetes. Penelitian dilakukan untuk mengamati efek  vitamin  E  pada  kemampuan  EPC  yang  dipapar glukosa  tinggi  dalam melepaskan  NO dan  menginduksi  migrasi  sel endotel.  Sel mononuklear diisolasi dari darah perifer subjek sehat.  Pada hari ke-7, kultur EPC diberikan glukosa normal (5 mM) dengan atau tanpa pemberian vitamin E 22 µM atau 50 µM sebagai kontrol, atau diberikan glukosa tinggi (22 mM) dengan  atau  tanpa  pemberian  vitamin  E  22  µM  atau  50  µM  selama  24  jam.  Fungsi  EPC  dinilai  dengan  mengevaluasi  migrasi HUVEC setelah pemberian supernatan EPC. Migrasi HUVEC dinilai dengan uji migrasi wound-healing. Konsentrasi NO dan H O   EPC diukur dengan uji kolorimetrik.  Superoksid EPC dinilai dengan uji NBT .  Pemberian glukosa tinggi mengakibatkan 2 2penurunan kemampuan EPC dalam menginduksi migrasi HUVEC, penurunan NO EPC, serta peningkatan superoksid dan H O   EPC.  Pemberian  vitamin  E  50  µM  dapat  menghambat  penurunan  kemampuan  EPC  dalam  menginduksi  migrasi 2 2HUVEC,  dan  efek  ini  terkait  dengan  konsentrasi  NO,  superoksid  dan  H O   EPC.  Pemberian  vitamin  E  dapat 2 2mempertahankan kemampuan EPC yang dipapar glukosa tinggi dalam melepaskan NO dan menginduksi migrasi HUVECs melalui  hambatan  peningkatan  superoksid  dan  H O   EPC.2 2Kata  Kunci:  EPC,  glukosa  tinggi,  NO,  ROS,  vitamin  E
Physalis minima Leaves Extract Induces Re-Endothelialization in Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction in Rats Nugrahenny, Dian; Permatasari, Nur; Rohman, Mohammad Saifur
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.898 KB) | DOI: 10.21776/ub.rjls.2017.004.03.6

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The administration of deoxy-corticosterone acetate (DOCA)-salt can induce oxidative stress leading to decrease the bioavailability of nitric oxide (NO), increase senescence of circulating endothelial progenitor cells (EPCs), thus contributing to endothelial dysfunction. This study was aimed to investigate the effects of Physalis minima L. leaves extract on serum NO levels, circulating EPCs number, and histopathology of tail artery endothelial cells in DOCA-salt-induced endothelial dysfunction in rats. Twenty-five male Wistar rats were randomly divided into five groups: rats without any treatment (normal), rats treated with DOCA (10 mg/kgBW s.c. twice weekly) and given 0.9% NaCl to drink ad libitum for 6 weeks, and DOCA-salt-induced rats orally supplemented with P. minima leaves extract at doses of 500, 1500, or 2500 mg/kgBW for 4 weeks. Serum NO levels were measured by colorimetry. The number of circulating EPCs (CD34+/CD133+ cells) was determined by flow cytometry. The tail artery sections were histologically processed with hematoxylin-eosin staining. DOCA-salt-induced rats showed significantly (p&lt;0.05) decrease in serum NO levels and circulating EPCs number compared to the normal. There was also more detached tail artery endothelial cells in DOCA-salt-induced rats. P. minima leaves extract at a dose of 500 mg/kgBW significantly (p&lt;0.05) increased serum NO level and circulating EPCs number, and also induced an optimal re-endothelialization in DOCA-salt-induced rats. P. minima leave extract dose-dependently increases NO bioavailability contributing to enhanced EPCs mobilization, thereby promoting re-endothelialization in DOCA-salt-induced endothelial dysfunction in rats.