MADE PHARMAWATI
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia

Published : 20 Documents
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Journal : Advances in Tropical Biodiversity and Environmental Sciences

Morphological and Anatomical Changes by Cochicine in Seedling of Impatiens balsamina L. Wiendra, Ni Made Sastriyani; Pharmawati, Made
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 2 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2019.v03.i02.p04

Abstract

Impatiens balsamina L. is a plant that is widely cultivated in Bali. The flower of this plant is used as a component of offering in Hindu religious ceremonies. The flower petals are thin and wilt easily, therefore genetic modification is needed to get better quality. This research aimed to study the effect of 0.01% colchicine with soaking periods of 4 hours, 8 hours, 12 hours and 24 hours to seedlings of I. balsamina. Seeds of I. balsamina were germinated and sprouted seeds were treated with 0.01% colchicine for 4 hours, 8 hours, 12 hours and 24 hours. The sprouted seeds were then transfer to polybag with soil media. The experiment was designed with randomized blok design with five replicates. Observations were done on plant height, length and width of leaf, stem diameter, number of stomata as well as observation on plant stem cortex cells. Results showed that colchicine reduced plant height at three weeks after planting, while plant stem diameter increased. Control plants had the smallest leaf length and width. The number of stomata decreased at plants derived from colchicine treated sprouted seeds and the lowest stomatal number observed was at soaking duration of 24 hours.
Comparison of DNA Yield from Different Plant Materials of Plumeria sp. (Apocynaceae) Martida, Vanesa; Pharmawati, Made
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2019.v03.i01.p03

Abstract

DNA extraction that gives good quantity and quality DNA is a basic step that must be completed for molecular studies, especially in DNA fingerprint imaging.  The aim of this research was to find out the better quality and quantity of DNA extracted from different plant materials of frangipani cultivars (Plumeria sp.). Leaves and flowers were collected from Taman Jepun, Denpasar Bali.  Fresh young leaves and flowers were used as plant materials as well as dried leaves (silica gel dried leaves) of Plumeria sp.  This research used CTAB buffer with modification as lysis buffer.  Purification techique used NucleoSpin? Gel and PCR Clean Up Kit. The results showed that the colour of DNA solution from fresh material was clear and the quantities of DNA from young fresh leaves were between 70-300 ng/?l. The DNA colour solution from flowers was also transparent with concentration between 0-40 ng/?l. DNA isolated from dry material resulted in brown solution with DNA quantity between 30-100 ng/?l and need to be purified to obtain clear DNA solution.