Endang Prangdimurti
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor

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PENGARUH MINUMAN DIPERKAYA CINNULIN TERHADAP MDA DAN SENYAWA INFLAMASI PLASMA PENDERITA DMT2 Putri S., Nawasari Indah; Prangdimurti, Endang; Zakaria, Fransiska R.
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 1 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.667 KB) | DOI: 10.6066/jtip.2016.27.1.51

Abstract

 Cinnamon has been used as a traditional treatment for thousands of years. Aqueous cinnamon extract namely cinnulin is known to have  positive effects on type-2 diabetes mellitus (T2DM). We aimed to study the effect of cinnulin enriched drink rich in polyphenols on plasma malondialdehyde (MDA), interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) in T2DM patients. 255 mg of cinnulin was mixed with tea extract into a 2.5 gram of instant drink with alumunium foil as the packaging material. A single blind study were performed on 33 respondents with T2DM from Dramaga District. Respondents were divided into three groups, i.e. group given cinnulin enriched drink (1), instant tea drink (2), and none of the products (control) (3). Group 1 and 2 were assigned to drink two sachetsof the products a day for 5 weeks. The MDA content of all groups remain unchanged after treatment although slightly increased (Wilcoxon, P>0.050) and no differences between groups was observed (P>0.050) suggesting that high phenolic content in the producst cause a reverse effect. Both inflammation marker (IL-6 and COX-2) decreased significantly for groups given cinnulin enriched drink and instant tea, whereas there is a significant increase for IL-6 and a significant decrease for COX-2 in control group. These results conclude that cinnulin enriched drink can be used as an antiinflammation drink for T2DM.
Potensi rumput laut: Kajian komponen bioaktif dan pemanfaatannya sebagai pangan fungsional Erniati, Erniati; Zakaria, Fransiska Rungkat; Prangdimurti, Endang; Adawiyah, Dede Robiatul
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Aquatic Sciences Journal, Vol. 3: No. 1 (April, 2016)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v3i1.332

Abstract

Rumput laut merupakan sumber daya hayati yang sangat berlimpah di perairan Indonesia. Namun demikian pemanfaatannya untuk pengolahan produk pangan sangat terbatas, terutama untuk produk pangan fungsional. Rumput laut berpotensi dikembangkan sebagai produk pangan fungsional karena mengandung zat gizi dan komponen bioaktif yang berkhasiat untuk kesehatan. Rumput laut mengandung sejumlah komponen bioaktif seperti senyawa fenolik, pigmen alami, polisakarida sulfat, serat dan komponen bioaktif lainnya yang telah diteliti berkhasiat untuk kesehatan. Untuk dapat dikembangkan sebagai produk pangan fungsional, rumput laut yang digunakan harus bebas dari cemaran logam berat dan bahan pencemar lainnya, harus mengandung komponen bioaktif dan zat gizi yang tinggi sehingga harus ada penerapan standar penanaman dan penanganan pasca panen yang baik di tingkat petani rumput laut. Selain itu Proses pengolahan pangan yang diterapkan tidak merusak komponen bioaktif yang terkandung dalam rumput laut. Optimalisasi pengolahan rumput laut sebagai produk pangan fungsional merupakan alternative pemanfaatan potensi rumput laut Indonesia yang dapat meningkatkan nilai ekonomi rumput laut dan yang lebih penting dapat menyediaakan akses pangan sehat bagi masyarakat luas.Seaweed is a living resource that is abundantly available in Indonesian water. However, its utilization in food processing is very limited, especially as functional food products. Seaweed has the potential to be developed as functional food products because it has nutrient and bioactive components that are beneficial for health. Seaweed has a number of bioactive components such as phenolic compound, natural pigment, polysaccharide sulphate, fiber and other bioactive components that has been studied to be advantageous for health. For a seaweed to be developed into functional food product, it must be free from heavy metal and other pollutant contamination, and must contain bioactive components and high nutrients, thus, a good cultivation and postharvest handling standard have to be applied in seaweed farmer level. Moreover, the food processing applied should not damage the bioactive component within the seaweed. Optimization of seaweed processing into functional food product is an alternative for seaweed potential utilization in Indonesia, which could improve the economic value of the seaweed, and more importantly it could provide access for healthy food for community.
FTIR-METABOLOMICS TO CORRELATE SORGHUM’S CHEMICAL PROFILE AND HCT-116 CYTOTOXICITY CHANGES DURING RICE-ANALOGUE PRODUCTION Yuliana, Nancy Dewi; Prangdimurti, Endang; Faridah, Didah Nur
Jurnal Teknologi dan Industri Pangan Vol. 29 No. 2 (2018): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.574 KB) | DOI: 10.6066/jtip.2018.29.2.110

Abstract

Rice-analogue (RA) made from sorghum (Sorghum bicolor L. Moench) has recently been popular in Indonesia for its potential as an alternative staple food besides rice. Sorghum has many phytochemicals with various functional properties including those which correlate to anticancer activity. The RA production in-volves several steps; polishing, milling, mixing, extruding, and oven drying. This study used FTIR based metabolomics to identify if these steps affect sorghum phytochemicals composition and its cytotoxicity acti-vity against HCT-116 cell lines in-vitro. It was discovered that sorghum cytotoxic activity was relatively sta-ble during the process. The RA final product (oven dried RA) showed higher activity (90.85%) as compared to other samples taken from previous step (2000 ppm dose). The correlation between FTIR profile and cytotoxic activity of RA was analyzed using one of the multivariate data analysis method namely orthogonal projection to the least square (OPLS). By comparing the OPLS data with FTIR data of compounds reported to be found in sorghum, it is shown that typical FTIR patterns for phenolic compounds particularly ferulic acids, p-coumaric acid, and procyanidins, as well as phytosterols, were highly correlated with RA?s cytotoxic activity. The signals were found to be dominant in the most active sample (oven dried RA). It can be concluded that sorghum phytochemicals responsible for its cytotoxic activity were not affected by processing steps, therefore RA is potential to be promoted as alternative func-tional staple food in Indonesia.
KANDUNGAN GIZI DAN MUTU PROTEIN TEPUNG BIJI KELOR TERFERMENTASI Sakinah, Ni'mawati; Prangdimurti, Endang; Palupi, Nurheni Sri
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 2 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.112 KB) | DOI: 10.6066/jtip.2019.30.2.152

Abstract

Moringa oleifera seed has the potential as a source of new food ingredients having high nutritional content, especially protein. The objective of this study was to evaluate the effect of fermentation toward biochemical composition and in vitro protein digestibility (IVPD) of Moringa seed flour. Fermentation was carried out by soaking the seeds at room temperature (30±2°C) for 24 and 48 h, either naturally (without starter addition) and with starter addition (i.e. commercial starter containing lactic acid bacteria/LAB). Unfermented and fermented seeds were processed into flour and their proximate composition, antitrypsin, tannin and IVPD were analyzed. The statistical methods used were ANOVA and Duncan's test at confi-dence level of 95%. The best treated flour was chosen using the De Garmo method and the amino acid profile was then analyzed. Protein digestibility-corrected amino acids (PDCAAS) were calculated to deter-mine the biological quality of proteins. The results showed that fermentation affected the changes in bio-chemical composition of the flour. Longer fermentation time could reduce the crude protein and antitrypsin content in both types of fermentation. On the other hand, there was an increase in tannin content during fermentation. The IVPD also increased by 75% at 48 h fermentation from the initial digestibility of raw seeds of 71%, thus increase in tannin content did not affect the IVPD. Natural fermentation of moringa seeds for 48-hour resulted in the best flour with IVPD and PDCAAS values of 75.33% and 0.18 (18.31%) respectively.
PENGARUH PERBEDAAN WAKTU PANEN TERHADAP KARAKTERISTIK KIMIA BIJI KECIPIR Setiawan, Rizki Dwi; Zakaria, Fransiska Rungkat; Sitanggang, Azis Boing; Prangdimurti, Endang; Adawiyah, Dede Robiatul; Erniati, Erniati
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 2 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.897 KB) | DOI: 10.6066/jtip.2019.30.2.133

Abstract

In Indonesia, the utilization of winged bean seeds as a food source is very limited. Currently there is inadequate information on the characteristics of the seeds, especially the chemical properties associated with its maturity. This research aimed to analyze the chemical properties of winged bean obtained from different harvesting time. Three different harvesting times were investigated, i.e. eight (K1), twelve (K2), and as six (6) weeks after the first flowering stage as a control. K1 and K2 were dried at 40°C (24 h) to mimic the conventional preparation of beans practiced in Indonesia, while K3 was unripe seeds commonly consumed fresh thus it is analyzed as fresh seeds. K1 and K2 have water content between 12.3-13.0% (wb), ash content 4.7-4.8% (db), lipid content 13.4-15.4% (db), protein content 38.9-40.7% (db), carbo-hydrate content 40.8-41.0% (db), total phenolic content 7.6 and 5.3 mg GAE/g (db), antioxidant activity (IC50) 558.3 and 511.1 µg/mL, starch content 25.6-29.1%, reducing sugar content 1.3-1.7 mg/g. Mean-while, the unripe winged bean seeds (K3) has water content of 75.5% (wb), ash content 5.0% (db), protein content 19.6% (db), carbohydrate content 68.4% (db), total phenolic content of 59.4 mg GAE/g (db), anti-oxidant activity (IC50) 485.6 µg/mL, starch content 7.2% and reducing sugar 5.4 mg/g. Based on these che-mical properties and time efficiency, harvesting winged bean at 8 weeks (K1) was sufficient to produce winged bean potential as protein source, as well as a potential functional foods with good antioxidant acti-vity, total phenolic content, low starch and reducing sugar.
POTENSI LACTOBACILLUS YANG DIISOLASI DARI AIR SUSU IBU UNTUK MENCEGAH DIARE [POTENTIAL OF LACTOBACILLUS ISOLATED FROM BREAST MILK TO PREVENT DIARRHEAE] Nuraida, Lilis; Hana, .; Hartanti, Apriliana W.; Prangdimurti, Endang
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.476 KB) | DOI: 10.6066/jtip.2012.23.2.158

Abstract

Some of Lactobacillus species isolated from breast milk are known to have antimicrobial activities, including against Escherichia coli. The aims of this study were to evaluate the antimicrobial activity of Lactobacillus species isolated from breast milk against enteropathogenic Escherichia coli strain K1.1 and the effectiveness of the Lactobacillus isolates to prevent diarrhea on rats (Sprague Dawley). The infective dose of E. coli K1.1 to induce diarrhea without causing death were also determined. Based on the antimicrobial activity there were three isolates of Lactobacillus that exhibited good inhibition againts E. coli K1.1, i.e. Lactobacillus rhamnosus R14, L. rhamnosus R23, and L. rhamnosus B16. Determination of E. coli infective dose showed that 108 CFU of E. coli K1.1 was sufficient to induce diarrhea on rat without causing death. The number of diarrhea rats and severity level in group treated with L. rhamnosus were lower than groups untreated with the Lactobacilli. This study showed that the three L. rhamnosus isolated from breast milk were able to prevent diarrhea due to infection of E. coli K1.1 when the Lactobacillus was regularly introduced prior to infection. L. rhamnosus R23 showed the best capabilities of preventing diarrhea in rats as compared to two other isolates of Lactobacillus. The incidence of diarrhea correlated with the number of lactobacilli in the feces. However when the period of diarrhea ceased, there were no difference in total lactobacilli and E. coli in the caecum, colon and feces between rats treated with L. rhamnosus and the control. This finding revealed the L. rhamnosus isolated from breast milk were potential for prevention of diarrhea when consumed regularly.
Crude Palm Oil Consumption As A Hepatoprotectors To Housewives In Bogor Perdani, Claudia Gadizza; Zakaria, Fransiska R; Prangdimurti, Endang
Jurnal Teknologi Pertanian Vol 17, No 2 (2016)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.905 KB)

Abstract

Kekurangan Vitamin A (KVA) di Indonesia masih merupakan masalah utama. Kekurangan vitamin A tingkat berat (rabun senja) telah jarang ditemui, tetapi tingkat KVA subklinis masih bisa ditemukan. Minyak sawit mentah (CPO) memiliki kandungan tinggi karotenoid sebagai sumber provitamin A. CPO secara alami berwarna merah karena mengandung karotenoid yang tinggi, dan belum dimanfaatkan di Indonesia. CPO mengandung karotenoid dan vitamin E yang berfungsi sebagai antioksidan bagi tubuh. Antioksidan dapat mengurangi kerusakan sel dengan menetralisir radikal bebas serta mencegah kerusakan lipid, protein, enzim, dan DNA. Tujuh puluh responden dipilih untuk konsumsi CPO selama 2 bulan dengan dosis ± 3.27 ml/hari. Plasma darah dari 22 responden yang sehat dianalisis dengan metode asam Tri Fluoro asetat (TFA), enzim aspartat Transaminase (AST), Alanine transaminase (ALT), Alkaline Phospatase (ALP) kit. Hasil penelitian menunjukkan 16 responden mengalami peningkatan jumlah retinol plasma dan penurunan tingkat aktivitas AST, ALT, dan enzim ALP. Penelitian ini menunjukkan bahwa CPO memiliki aktivitas antioksidan yang dapat meningkatkan kesehatan hati, sehingga dapat menjadi alternatif sumber provitamin A untuk menangani kekurangan vitamin A di Indonesia
PRODUKSI PEKTIN BERMETOKSIL RENDAH DARI KULIT JERUK NIPIS (Citrus aurantifolia Swingle) SECARA SPONTAN MENGGUNAKAN PELARUT AMONIUM OKSALAT DAN ASAM Usmiati, Sri; Mangunwidjaja, Djumali; Noor, Erliza; Richana, Nur; Prangdimurti, Endang
Jurnal Penelitian Pascapanen Pertanian Vol 13, No 3 (2016): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v13n3.2016.125-135

Abstract

Pengembangan teknologi ekstraksi pektin bermetoksil rendah terus dieksplorasi karena tanaman sumber yang relatif terbatas. Tujuan penelitian adalah untuk memperoleh pektin bermetoksil rendah langsung dari ekstraksi kulit jeruk nipis (Citrus aurantifolia Swingle). Penelitian didahului oleh dua tahap penelitian pendahuluan menggunakan rancangan acak lengkap yang masing-masing bertujuan memperoleh suhu ekstraksi (tahap pertama) dan lama waktu ekstraksi (tahap kedua) terbaik untuk digunakan sebagai kondisi proses ekstraksi pada penelitian utama. Desain penelitian utama menggunakan rancangan acak lengkap pola faktorial dengan tiga ulangan. Faktor pertama adalah tipe pelarut (P): P1 (amonium oksalat+asam oksalat, asam sitrat) dan P2 (amonium oksalat+asam oksalat, asam klorida), dan faktor kedua pH (K): K1 (pH 1) dan K2 (pH 2), serta sebagai pembanding adalah menggunakan pelarut P0 [asam sitrat, asam klorida; pH 2,0]. Ekstraksi suksesif dua tahap menggunakan suhu 100oC dilakukan selama 45 menit dengan pelarut amonium oksalat+asam oksalat, dilanjutkan 10 menit menggunakan pelarut asam sitrat atau asam klorida. Parameter pengukuran meliputi rendemen, derajat esterifikasi (DE), kadar asam uronat, kadar metoksil, kadar air, kadar abu, serta berat ekivalen. Dari hasil penelitian diketahui bahwa interaksi tipe pelarut dan pH pada perlakuan P2K2 (amonium oksalat+asam oksalat, asam klorida) dapat langsung menghasilkan pektin bermetoksil rendah dari kulit jeruk nipis dengan nilai DE 45,77%, kadar metoksil 1,53% dan kadar abu 4,77%. Pelarut amonium oksalat+asam oksalat (t1) dan asam klorida (t2) (P2) pada kedua tingkat pH menghasilkan rendemen pektin 10,0%, kadar AUA 19,21%, dan nilai BE 1878,82. Kadar air pektin sebesar 7,91% dihasilkan dari tingkat pH 2 (K2) pada kedua tipe pelarut. Untuk memperoleh pektin bermetoksil rendah dari kulit jeruk nipis direkomendasikan menggunakan pelarut P2K2 (amonium oksalat+asam oksalat, asam klorida; pH 2) pada suhu ekstraksi 100oC.English Version AbstractSpontaneously production of low methoxyl pectin from peel of Citrus aurantifolia Swingle using solvent of ammonium oxalate and acidThe development of extraction technology of low methoxyl pectin/LMP continue to be explored caused by limited the plant source. The research objective was to obtain low methoxyl pectin directly from the pectin extraction of lime peel (Citrus aurantifolia Swingle). The study was preceeded by a twostage preliminary researches using a completely randomized design, each of which was to obtain the best temperature (first stage) and duration of extraction (second stage) to be used as a condition of extraction process in the primary research. The primary research used factorial completely randomized design with three replications. The first factor was solvent type (P): P1 (ammonium oxalate+oxalic acid), citric acid) and P2 (ammonium oxalate+oxalic acid, hydrochloric acid), and the second factor was pH (K): K1 (pH 1) and K2 (pH 2), as well as control was solvent P0 [citric acid (t1), hydrochloric acid (t2); pH 2.0]. Successive two-stage extraction on 100°C was done for 45 minutes using ammonium oxalate+oxalic acid followed by 10 minutes using citric acid or hydrochloric acid. Measurement parameters included yield, degree of esterification (DE), anhidrouronic acid/AUA levels, methoxyl content, moisture content, ash content, and equivalent weight. From the results of research, the interaction between solvent tipe and acidity level of P2K2 (ammonium oxalate+oxalic acid, hydrochloric acid; pH 2) could directly produce LMP from extraction of lime peel characterized by DE of 45.77%, methoxyl content of 1.53% and ash content of 4.77%. Effect of solvent of ammonium oxalate+oxalic acid, hydrochloric acid (P2) at both pH levels resulted pectin yield of 10.0%, AUA of 19.21%, and equivalent weight of 1878.82. The pectin moisture of 7.91% was produced from acidity of pH 2 (K2) on both type of solvent. To obtain LMP from peel of Citrus aurantifolia Swingle it was recommended to use the extraction solvent of P2K2 (ammonium oxalate+oxalic acid, hydrochloric acid; pH 2) on temperature of 100oC.
Alergenisitas Sistem Glikasi Isolat Protein Kedelai-Fruktooligosakarida Suseno, Rahayu; Palupi, Nurheni Sri; Prangdimurti, Endang
Agritech Vol 36, No 4 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.811 KB) | DOI: 10.22146/agritech.16770

Abstract

Food allergy is an immunological response caused by allergens contained in food. Soybean is one of the eight kinds of food products that can cause allergies. Genetically modified food crops that are most widely produced worldwide is soybean (47 % worldwide). Genetically Modified Organisms (GMO) products is concerned may increase the allergenicity of the  product. The aims of the research were to study the allergenicity of GMO and non-GMO Soy Protein Isolates (SPI) and the glycation effect to allergenicity of SPI. GMO and non-GMO SPI were glycated with fructooligosaccharides (FOS) through the Maillard reaction in liquid systems. Allergenicity was determined qualitatively using immunoblotting and quantitatively using Enzyme-Linked Immunosorbent Assay (ELISA). The glycation degree of GMO and non-GMO SPI can increase up to 75.03 % and 73.50 % in the liquid system. There were 9 protein allergens in GMO soybean and 8 protein allergens in non-GMO soybean. The glycation reaction could reduce protein allergens in GMO and non-GMO SPI up to 91.69 % and 87.07 %.ABSTRAKAlergi pangan merupakan sebuah respon imunologis yang disebabkan oleh alergen yang terdapat pada pangan. Kacang kedelai merupakan satu dari delapan jenis bahan pangan yang sering menyebabkan alergi. Tanaman pangan hasil rekayasa genetika (GMO) yang banyak diproduksi di dunia adalah kacang kedelai yaitu sekitar 47 %. Produk GMO dikhawatirkan dapat meningkatkan alergenisitasnya. Penelitian ini bertujuan untuk mempelajari tinggat alergenisitas antara Isolat Protein Kedelai (IPK) GMO dan non-GMO serta pengaruh glikasi terhadap alergenisitas IPK. IPK GMO dan non-GMO diglikasi dengan fruktooligosakarida melalui reaksi Maillard dengan sistem cair. Alergenisitas diukur secara kualitatif menggunakan immunobloting dan secara kuantitatif menggunakan Enzyme-Linked Immunosorbent Assay (ELISA). Peningkatan derajat glikasi IPK GMO dan non-GMO pada sistem cair masing-masing memperlihatkan hasil 75,03 % dan 73,50 %. Terdapat 9 protein alergen pada kacang kedelai GMO dan 8 protein alergen pada kacang kedelai non-GMO. Reaksi glikasi dapat mengurangi alergen pada kacang kedelai GMO dan non-GMO hingga 91,69% dan 87,07%.
POTENSI ANTIOKSIDAN GEL DAN DAUN KACAPIRING (GARDENIA JASMINOIDES ELLIS) Widnyana Yoga, IB Ketut; Andarwulan, Nuri; Prangdimurti, Endang
Prosiding Seminar Nasional MIPA 2011: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2011
Publisher : Prosiding Seminar Nasional MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract: Leaf of kacapiring (Gardenia jasminoides Ellis) is a part of plant which is having component that can form gel. It has chlorophyll pigment and phenolic compound. The aims of this research were to identificate bioactive compound in leaves and gel which has antioxidant potency. Chlorophyll total was analyzed by spectrophotometer and its derivations of aceton extract (99.9%) by thin layer chromatography (TLC), meanwhile phenolic total and antioxidant capacity were analyzed by spectrophotometer. The result showed that bioactive component of kacapiring leaves and gel were chlorophyll total of 4926.25+190.31 and 1166.86+8.73  mgKg-1db. Both of them had 5 fractions by acetone extract, i.e. chlorophyll a, chlorophyll b, lutein (chlorophyll derivates), feofitine and carotene. Phenolic total in leaves and gel contained 5215.91+2.97 and 2648.16+56.22 GAE/g db, and antioxidant capacity had 1.5 x 10-1+0.00 and 3.1x10-3 +0.00 mM TEAC/mg dw respectively