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3-ACETYL -2,5,7-TRIHYDROXY-1,4-NAPHTALENEDIONE, AN ANTIMICROBIAL METABOLITE FROM THE CULTURE OF ENDOPHYTIC FUNGUS COELOMYCETES TCBP4 FROM Tinospora crispa Praptiwi, Praptiwi; Jamal, Yuliasri; Fathoni, Ahmad; Nurkanto, Arif; Agusta, Andria
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 3 Sep (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Abstract Isolation, identification and testing of antimicrobial activity of secondary metabolites of endophytic fungal culture TCBP4 isolated from bratawali (Tinospora crispa) has been performed. The fungus TCBP4 was cultivated in Potato Dextrose Broth (PDB) for 1 month, media and fungi extracted with ethyl acetate. The extract was separated and purified by several chromatographic techniques, from which 9 fractions were obtained. Fraction 3e was purified again and was obtained 6 fractions (3e1-3e6). Fractions 3e3-3e6 were tested against bacteria isolates Staphylococcus aureus Bacillus subtilis,Eschericia coli and yeast isolate Candida albicans by microdillution method. Antimicrobial activity test result showed that fractions 3e3-3e5 had better antibacterial activity compared to chloramphenicol as commercial antibiotic. It was indicated by MIC value of the fractions was lower (8 ug/ml) compared with the antibiotic chloramphenicol (16 ug/ml). Fraction 3e3 had better antifungal activity compared to commercial antifungal nystatin and cabisidin against C. albicans. GC-MS analysis showed that the chemical constituent of 3e5 fraction was identified as 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Key words : Tinospora crispa, endophytic fungi, isolation, identification, antimicrobial Abstrak Isolasi, identifikasi dan uji aktivitas antimikrobia terhadap metabolit sekunder dari kultur jamur endofit TCBP4 yang diisolasi dari tumbuhan bratawali (Tinospora crispa). Jamur endofit TCBP4 dikultivasi pada media Potato Dextrose Broth (PDB) selama 1 bulan, selanjutnya media dan jamur diekstrak dengan pelarut etil asetat. Ekstrak dipisahkan dan dimurnikan dengan beberapa teknik kromatografi sehingga diperoleh 9 fraksi. Fraksi 3e dimurnikan kembali dan diperoleh 6 fraksi (3e1-3e6). Fraksi 3e3-3e6 diuji aktivitas antibakterinya terhadap beberapa isolate bakteri Staphylo-coccus aureus Bacillus subtilis, Eschericia coli dan isolate khamir Candida albicans dengan metode mikrodilusi. Hasil uji antimikrobia menunjukkan bahwa fraksi 3e3-3e5 mempunyai aktivitas antibakteri lebih baik bila dibandingkan dengan chloramfenikol, dimana nilai MIC dari fraksi (8 ug/ml) lebih rendah dari antibiotika chloramfenikol (16 ug/ml). Fraksi 3e3 mempunyai aktivitas antijamur lebih baik bila dibandingkan dengan antijamur komersial nistatin dan kabisidin terhadap  C. albicans. Analisis GC-MS menunjukkan bahwa komponen kimia dari fraksi 3e5 diidentifikasi sebagai 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Kata kunci : Tinospora crispa, jamur endofit, isolasi, identifikasi, antimikrobia
UJI AKTIVITAS ANTIBAKTERI DAN SITOTOKSIK EKSTRAK METANOL AGLAIA SILVESTRIS (M.ROEMER) MERR. Praptiwi, Praptiwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2532

Abstract

The antibacterial and sitotoxic activities of ganggo (Aglaia silvestris (M.Roemer) Merr. methanol extract were determined in this study. Antibacterial in-vitro test of ganggo methanol extract was exposed to Gram positive (Staphylococcus aureus ATCC 25923, Streptococcus agalactiae ATCC 8190, and Streptococcus sp.) and Gram negative (Salmonella typhii NCTC 786 E, Eschericia coli ATCC 25922, and Pseudomonas pseudomallei ATCC 15682) bacteria. The extract concentrations were 50, 25 and 12.5%, and done in triplicate. The growth inhibition area of extract was compared to those of standard antibiotic (10 unit ampicilin). Cytotoxic test of ganggo extract was done utilizing Brine Shrimp Lethality Test (BST) with Artemia salina.The result showed that growth inhibition area of 12.5% ganggo methanol extract to P. pseudomallei (19 mm) was wider than that of 10 unit ampicilin (0 mm). It showed that P. pseudomallei was sensitive to ganggo methanol extract. The result of BST showed that LC50 of ganggo extract was 345.44 ppm. It was concluded that ganggo methanol extract had antibacterial effects on some bacteria isolates and had cytotoxic effects with LC50 345.44 ppm.
SKRINING AWAL AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SEMUT (INSECTA: FORMICIDAE) DARI GARUT - JAWA BARAT Efendy, Oscar; Fathoni, Ahmad; Praptiwi, Praptiwi; Royyani, Mohammad Fathi; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3621

Abstract

Studies on the therapeutic use of insects and insect products have been neglected compared to the use of other animals or plants.This study aims to determine the antibacterial and antioxidant potential of ants extracts. Preliminary study related to antibacterial and antioxidant screeningof 17 extracts of ant colonies that belongs to 8 species were performed by Thin Layer Chromatography (TLC)-Bioautography. Antioxidant activity was measured by DPPH free radical scavenging method. The antibacterial activity was done against three pathogenic bacteria, i.e Bacillus subtilis InaCC B-1, Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5 were performed by non-eluted TLC-autobiography assay. The minimum inhibitory concentration (MIC) and IC50 values of DPPH radical scavenging activity of active extracts were determined by microdilution in 96-well microplate. The results showed 6 extracts active against B.subtilis, 5 extracts active against S.aureus. The lowest MIC value was 512 µg/ ml.  Ten extracts had the antioxidant activity with various IC50 values. The extracts of ants might be used as bioactive resources for antibacterial and antioxidant.  
IDENTIFIKASI SENYAWA KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK PIPER SP. ASAL PAPUA Priyono, Sumarnie; Praptiwi, Praptiwi
Jurnal Teknologi Lingkungan Vol 10, No 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jtl.v10i3.1473

Abstract

The aim of this study was to identity the chemical compounds of the piper sp.Fruit originated from Papua, that has been used as ?sirih pinang?(chew betle)and to determine the antibacterial activity of hexane and chloroform extraxt ofpiper sp. to Gram + bacteria isolates(Bacillus subtilis, Listeria monocytogenesdan Staphylococcus aureus) and Gram ? bacteria isolates(Salmonellatyphimurium, Eschericia coli and Pseudomonas pseudomallei). The extractconcentration were 50 % and 25 %, and every treatment consisted of 3replications. The bacteriae growth media was Mueller Hinton Agar(MHA). Theresult of phytochemical screening of Piper sp.fruit(ether, alcohol,and waterextracts)showed that it contained esential oil, lipid,emodol,tannin,reducingsugar, anthrasenoid,polyuronide,glucocide,steroid glycoside.
SUMBANGAN ALL-TRANS ASAM RETINOAT (ATRA) BAGI PENYEMBUHAN PERIODONTITIS Praptiwi, Praptiwi; Muis, Siti Fatimah; Hadisaputro, Soeharyo; Suryono, Suryono
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTAll-trans retinoic acid (ATRA) contribution to periodontitis healingBackground: In inflammation and infection of periodontal ligament i.e periodontitis, collagen fiber is damaged. All-trans retinoic acid (ATRA), metabolite of vitamin A, has potency to depress damage in inflammation. Indonesia with high vitamin A deficiency, facing to delayed recovery of periodontitis. The disease is vastly suffered by common people in this country. The study aimed to know the collagen status of periodontal ligament suffering periodontitis treated with tetracycline and ATRA supplementation.Method: The experimental study using post test only design in 12 adult male Wistar rat was done in 7 days. The animal study were induced periodontitis through Porphyromonas gingivalis inoculation. Tetracycline 90 mg/kg was given, beside ATRA supplementation of 10 mg/kg and 5 mg/kg. Four experimental groups were chosen randomly. Group of periodontitis + tetracycline was the control of the study. The independent variables were ATRA supplementation of those high and low dose. The dependent variable was collagen status. Collagen status consist of degradation and synthesis of collagen, indicated by mRNA MMP-2 and mRNA P1CP respectively. They were measured by reversed transcriptase polymerizing chain reaction and thin layer chromatography. All-trans retinoic acid concentration was measured using high performance liquid chromatography. The dataanalyzed using t-test, Oneway ANOVA, post hoc duncan and least significant differences.Result: In group receiving 10 mg ATRA/kgBB, the ATRA level was highest but the collagen degradation was lower although not significant compared to the low dose ATRA group. In both groups the degradation was significantly lower than the control group (p=0.007). The synthesis of collagen in the low dose ATRA group was higher than the high dose ATRA group and the control group.Conclusion: High dose of ATRA supplementation contribution to less degradation of collagen, but in term of collagen synthesis, low dose ATRA supplementation gives better result.Keywords: ATRA supplementation, collagen degradation, periodontitis ABSTRAKLatar belakang: Pada peradangan dan infeksi kronik ligamentum periodontal yaitu periodontitis, terjadi kerusakan serat kolagen. All-trans asam retinoat (ATRA), metabolit vitamin A, berpotensi menekan kerusakan pada peradangan. Tingginya defisiensi vitamin A di Indonesia, menghambat proses penyembuhan periodontitis yang banyak diderita masyarakat luas. Penelitian bertujuan mengetahui status kolagen ligamentum periodontal periodontitis yang mendapat pengobatan tetrasiklin dan suplementasi ATRA.Metode: Studi eksperimental dengan rancangan post test only pada 12 tikus Wistar jantan dewasa telah dilakukan selama 7 hari. Hewan coba diinduksi periodontitis melalui inokulasi bakteri Porphyromonas gingivalis. Diberikan tetrasiklin 90 mg/kg, juga suplementasi ATRA 10 mg/kg dan 5 mg/kg. Empat kelompok studi dipilih secara acak. Kontrol adalah kelompok sakit + tetrasiklin. Variabel bebas adalah suplementasi ATRA dosis tinggi dan dosis rendah, variabel tergantung adalah status kolagen. Status kolagenmencakup degradasi dan sintesis, berturut-turut dengan petanda mRNA MMP-2 dan mRNA P1CP, diukur menggunakan reverse transcriptase polymerizing chain reaction dan thin layer chromatography. Kadar ATRA diukur dengan high performance liquid chromatography. Data dianalisis dengan uji t, Oneway ANOVA, post hoc Duncan dan least significant differences.Hasil: Pada kelompok yang menerima suplementasi ATRA 10 mg/kgBB kadar ATRA tertinggi, tetapi degradasi kolagennya lebih rendah walau tidak bermakna. Kedua kelompok sumplementasi degradasi kolagennya lebih rendah secara bermakna dibanding kelompok kontrol (p=0,007), dalam sintesis kolagen,kelompok yang mendapat suplementasi ATRA 50 mg/kgBB sintesisnya lebih baik dibanding kelompok yang mendapat ATRA 10 mg/kgBB maupun kelompok kontrol.
UJI ANTIBAKTERI Lasianthus (RUBIACEAE) SEBAGAI TUMBUHAN BERKHASIAT OBAT DAN UPAYA PERBANYAKANNYA Purwantoro, R. Subekti; Siregar, Hartutiningsih-M.; Sudarmono, Sudarmono; Praptiwi, Praptiwi
Buletin Kebun Raya Vol 13, No 2 (2010): Buletin Kebun Raya Vol. 13 (2) July 2010
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Lasianthus, a member of the family Rubiaceae has potential for medicinal plants. The aims of the research were to find out the potential antibacterial activity of Lasianthus leaf extract and to investigate the effectiveness of plant propagation by stems cutting. The species tested were Lasianthus laevigatus Blume, L furcatus (Miq.) Bremek and L. cf. obscurus Blume. The extracted leaves in n-hexana, ethyl acetate and methanol were tested in vitro against Staphylococcus aureus and Escherichia coil activities that grown on medium of Mueller-Hinton agar. The methanol extracts of each plant material showed significant antibacterial activities on S. aureus and E. coll. Antibacterial activity increased with increasing concentration of the extracts. Plant propagation by stems cutting were done as a first step of conservation effort for respective species. Stems cutting of L. laevigatus, L. purpureus, L. furcatus, L. acuminatus, L rhinocerotis. and L. cf. obscurus with length of 10 cm were applied with Rotoon F to stimulate root growth. The result of plant propagation research showed that only the stems cutting of L. cf. obscurus grew well although roots did not grow until 4 months.
EFEKTIVITAS METODE KOOPERATIF TIPE GI DAN STAD DITINJAU DARI KEMAMPUAN AWAL Praptiwi, Praptiwi; Handhika, Jeffry
Jurnal Penelitian Pembelajaran Fisika Vol 3, No 1/april (2012): JP2F
Publisher : Jurnal Penelitian Pembelajaran Fisika

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Abstract

Penelitian ini bertujuan untuk mengetahui: 1) Perbedaan penggunaanmetode pembelajaran kooperatif tipe GI dan STAD terhadap prestasibelajar fisika. 2) Perbedaan kemampuan awal tinggi dan kemampuanawal rendah terhadap prestasi belajar fisika. 3) Interaksi antarametode pembelajaran dan kemampuan awal siswa terhadap prestasibelajar fisika. Jenis penelitian ini adalah eksperimen. Populasi dalampenelitian ini adalah semua siswa SMPN 1 Barat. Sebagai sampeldiambil dua kelas sebanyak 68 siswa yang kemudian dijadikan kelaseksperimen I dan kelas eksperimen II. Kelas VIIIB sebanyak 34 siswasebagai kelas eksperimen I diberikan pendekatan menggunakanmetode pembelajaran kooperatif tipe GI sedangkan kelas VIIIDsebanyak 34 siswa sebagai kelas eksperimen II diberikan pendekatanmenggunakan metode pembelajaran kooperatif tipe STAD denganteknik pengambilan sampel cluster random sampling. Teknik analisisdata yang digunakan adalah analisis variansi (anova) dua jalan.Hasilpenelitian dengan α = 5% dapat disimpulkan bahwa: (1) Metodekooperatif tipe GI lebih baik daripada metode kooperatif tipe STADdengan Fhitung = 92,77. (2) Siswa dengan kemampuan awal tinggimempunyai prestasi belajar fisika yang lebih baik dibandingkandengan siswa yang mempunyai kemampuan awal rendah dengan Fhitung = 4,199. (3) Ada interaksi antara metode pembelajaran dankemampuan awal siswa terhadap prestasi belajar fisika (F hitung =5,16), metode GI dan STAD cenderung berinteraksi pada kemampuanawal rendah.Kata kunci : Metode GI, STAD, Kemampuan Awal, Prestasi Belajar
PENAPISAN FITOKIMA DAN BILANGAN PEROKSIDA (POV) TIGA JENIS TUMBUHAN FAMILI MORACEACE (Artocarpus sp., A.elasticus, dan Ficus sp.) DARI TAMAN NASIONAL BOGANI NANI WARTABONE (SULUT) Harapini, Mindarti; Praptiwi, Praptiwi
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3804.176 KB) | DOI: 10.14203/beritabiologi.v6i4.3456

Abstract

The  aim of study were to determine the chemical compounds and peroxide values (POV) of methanol extract of three plants species belongs to Moraceae family (Artocarpus (Artocarpus sp., A.elasticus, dan Ficus sp.). Their chemical compounds were identified by Cuiley method, while peroxide value was done by iodometri titration. Methanol extract percentage of Artocarpus sp., A.elasticus, dan Ficus sp. were 20.80%, 3.10% and 4.10% respectively. All of the extract tested contained essential oil, sterol and triterpenoid, aglicon flavonoid, tannin, flavonoid glicoside and saponin. POV of Artocarpus sp., A.elasticus, dan Ficus sp. were 325.24, 306.25 and 1580.07 respectively and POV of q tocopherol was 363.96. So, it can be concluded taht all of three methanol extract act as reductor, because their POV less that POV of q tocopherol and might be act antioxidant. These correalted with their chemical compounds such as flavonoid, tannin and peroxide sugar.
EFEKTIVITAS METODE KOOPERATIF TIPE GI DAN STAD DITINJAU DARI KEMAMPUAN AWAL Praptiwi, Praptiwi; Handhika, Jeffry
Jurnal Penelitian Pembelajaran Fisika Vol 3, No 1/april (2012): JP2F
Publisher : Pendidikan Fisika Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jp2f.v3i1/april.384

Abstract

Penelitian ini bertujuan untuk mengetahui: 1) Perbedaan penggunaanmetode pembelajaran kooperatif tipe GI dan STAD terhadap prestasibelajar fisika. 2) Perbedaan kemampuan awal tinggi dan kemampuanawal rendah terhadap prestasi belajar fisika. 3) Interaksi antarametode pembelajaran dan kemampuan awal siswa terhadap prestasibelajar fisika. Jenis penelitian ini adalah eksperimen. Populasi dalampenelitian ini adalah semua siswa SMPN 1 Barat. Sebagai sampeldiambil dua kelas sebanyak 68 siswa yang kemudian dijadikan kelaseksperimen I dan kelas eksperimen II. Kelas VIIIB sebanyak 34 siswasebagai kelas eksperimen I diberikan pendekatan menggunakanmetode pembelajaran kooperatif tipe GI sedangkan kelas VIIIDsebanyak 34 siswa sebagai kelas eksperimen II diberikan pendekatanmenggunakan metode pembelajaran kooperatif tipe STAD denganteknik pengambilan sampel cluster random sampling. Teknik analisisdata yang digunakan adalah analisis variansi (anova) dua jalan.Hasilpenelitian dengan ?Ä?? = 5% dapat disimpulkan bahwa: (1) Metodekooperatif tipe GI lebih baik daripada metode kooperatif tipe STADdengan Fhitung = 92,77. (2) Siswa dengan kemampuan awal tinggimempunyai prestasi belajar fisika yang lebih baik dibandingkandengan siswa yang mempunyai kemampuan awal rendah dengan Fhitung = 4,199. (3) Ada interaksi antara metode pembelajaran dankemampuan awal siswa terhadap prestasi belajar fisika (F hitung =5,16), metode GI dan STAD cenderung berinteraksi pada kemampuanawal rendah.Kata kunci : Metode GI, STAD, Kemampuan Awal, Prestasi Belajar
SKRINING BEBERAPA JAMUR ENDOFIT TUMBUHAN DARI PULAU ENGGANO, BENGKULU SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN [Screening of Plant Endophytic Fungi from Enggano Island, Bengkulu for Antibacterial and Antioxidant Activites] wulansari, dewi; Putra, Aldho Pramana; Ilyas, Muhammad; Praptiwi, Praptiwi; Fathoni, Ahmad; Palupi, Kartika Dyah; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3260.703 KB) | DOI: 10.14203/beritabiologi.v15i3.2259

Abstract

Fungal endophytes have been known as sources for bioactives with high chemical structure variability. This study aimed to screen extracts of some endophytic fungi associated with plants from Enggano Island as antioxidant and antibacterial using thin layer chromatography (TLC) bioautography method. Antibacterial ativity was performed against Escherichia coli and Staphylococcus aureus. Seventeen out of 22 extracts showed growth inhibition activity against both bacteria, while 16 extracts were active as DPPH free radical scavenging agents. Further determination on IC50 value of four prominent extracts revealed that fungal endophytes AK3018-1, FC-1, KC-4, and SO-3 have IC50 value of 85, 84, 704, and 347 ?g/ml, respectively in DPPH radical scavenging method. Extracts of fungal endophytes CR-3, CS-2, and SM-2 showed prominent antibacterial activity among other extracts, indicated by wide clear white zone around the spot. Further evaluation on minimum inhibitory concentration (MIC) of those three extracts by microdilution method showed that CR-3, CS-2, and SM-2 have MICs value of 512, >512, and 64µg/ml, respectively against S. aureus.Fungal endophytes AK3018-1, FC-1, KC-4, SO-3, CR-3, CS-2, and SM-2 were respectively isolated from Dioscorea bulbifera tuber, Fibraurea chloroleuca twig, Knema cinerea twig, Smilax odoratissima stem, Cryptocarya sp. twig, Calophyllum soulattri twig, and Smilaxmacrophylla stem.