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Pengaruh Polymorphisme PvuII Gen Reseptor Estrogen Alfa terhadap Kadar Kalsium dan Phosphat Serum pada Wanita Postmenopause Prasetyastuti, Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 26, No 1 (2010)
Publisher : Berita Kedokteran Masyarakat (BKM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.478 KB)

Abstract

Background: Estrogen has an important role in bone metabolism. The hypoestrogenemia condition occurring in postmenopausal women may affect bone metabolism. The mechanism action of estrogen to prevent the activity of osteoclast in bone resorption was mediated especially by estrogen receptor alpha gene (ESR1). The PvuII polymorphism that occurs in ESR1 gene may alter their expression and  function.Objectives: the aim of this research was to determine the difference of serum calcium and phosphate level in postmenopausal women with PvuII polymorphism of the Estrogen receptor alpha geneMethod: This was cross sectional design. The subjects are postmenopausal women 50-70 year old .Twenty seven subject divided into two group, group I consisted of 15 women with genotype PP,Pp and group II consisted of 12 with genotype pp. polymorphism were assessed by Polymerase Chain Reaction Restriction Fragmen Length Polymorphism(PCR-RFLP) technique. Determination of serum calcium and phosphate level used spectrophotometer. The data was analyzed with Independent sample t-test.Results: The serum calcium level are 8,15 ± 1,35 mg/dl and 8,13 ± 1,58 mg/dl in genotype PP,Pp and pp respectively. The serum phosphate level are 3,8 ± 0,47 mg/dl and 4,24 ± 0,86 mg/dl in genotype PP,Pp and pp respectivelyConclusion: there were no statistically significant differences in serum calcium and phosphate level among genotype PP,Pp with pp  ESRI gene(p>0,05) PvuII polymorphism of ESRI gene did not influence serum calcium and phosphate level in postmenopausal women.Keywords: postmenopausal women, estrogen receptor polymorphism, serum calcium, serum phosphate
Korelasi Antara Kadar Vitamin C dengan Kadar Methemoglobin Darah Lansia di Kecamatan Pakem Kabupaten Sleman Prasetyastuti, Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 25, No 1 (2009)
Publisher : Berita Kedokteran Masyarakat (BKM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.427 KB)

Abstract

Background: Oxidative damage  by  free  radical can  cause  aging  process. Erythrocyte  as an  oxygen –  carrying agent  has high  risk  of  oxidative  injury which can  inducemethemoglobin  production.  Vitamin C  is  an  exogen antioxidant  that work  in  aqueous  phase  like  blood  and  can  react   with    free  radical before  they  initiate  the oxidative  injury.Objectives: The  aim of  this study was  to  find out whether  there  is possibility  of correlation  between  vitamin C and methemoglobin  level  in blood of elderly  living  in Pakem DistrictMethod:  This was  an observational  research with  cross  sectional  design,  in which  32  elderly  from  Pakem District were  subjects.  The  blood  sample was  taken  from  cubital  vein.  Determination  of   vitamin C  and methemoglobin  level  used  spectrophotometer. Pearson Correlation was  employed  to  analyze  the  data.Results: The vitamin C and methemoglobin level of the elderly 0,43 + 0,25 mg/dl  and  0,035 +  0.02% respectively. The  correlation between  vitamin C  and methemoglobin  level was  not significant    (  p =  0.771).Conclusion: The  vitamin C was not  correlated with  the methemoglobin  level.Keywords: vitamin C, methemoglobin,  elderly,  free  radica
Vitamin E and MDA Concentrations in Plasma of Healthy Young Adult, Elderly and Pregnancy Asjari, Sri Rahajoe; Prasetyastuti, Prasetyastuti; N.A., Zainal Arifin; Ngadikun, Ngadikun; Manampiring, Aalda Ellen; Sudarmo, Risanto Siswo; Sofoewan, Sulchan
Indonesian Food and Nutrition Progress Vol 10, No 2 (2003)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.104

Abstract

Vitamin E is one of essential micronutrients. It is an antioxidant which potentially protects bioinolecules against oxidative damage. One ofparameters ofoxidafive damage is MDA or lipid peroxide. In this study, concentrations of plasma vitamin E as an antioxidant and MDA as one of oxidative damage markers were measured. The subjects consisted offive groups: (I) male medical students (young adult), (II) female medical students (young adult), (III) nursery inhabitants in Yogyakarta province (Abiyoso), (IV) nursery inhabitants in Manado, North Sulawesi Province (Senja Cerah), as the old ages, and (V) pregnant women within third trimester (of Prenatal Care Unit in Sardjito Hospital). The concentrations of Vitamin E (mg/dl) and MDA (nmol/ml) were as follows: Young Adult Male (n= 28) 20,8 + 3,8 and 0,190 + 0,057 ; Young adult Female (n-28) 21,9 + 4,5 and 0,123 + 0,057 ; Old age Yogyakarta (n=41) 30,6 + 10,4 and 0,291 + 0,062 ; Old age Manado (n=40) 26,5 + 4,5 and 0,391 + 0,081 ; Pregnant Women (=28) 39,9 + 11,3 and 0,165 -f 0,067. Groups of people who were hyperlipemic tend to have high concentration of Vitamin E, while group IV who eat less vegetable protein and more PUFA tend to have higher MDA concentration.
Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus oktiyani, neni; Sunarti, sunarti; Prasetyastuti, Prasetyastuti; Cahyono, Jujuk Anton
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (995.684 KB) | DOI: 10.31964/mltj.v1i1.4

Abstract

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats Ayunda, Rahmah Dara; Prasetyastuti, Prasetyastuti; Hastuti, Pramudji
Indonesian Journal of Pharmacy Vol 30 No 3, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (855.702 KB) | DOI: 10.14499/indonesianjpharm30iss3pp180

Abstract

Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats. 
Pengaruh Polymorphisme PvuII Gen Reseptor Estrogen Alfa terhadap Kadar Kalsium dan Phosphat Serum pada Wanita Postmenopause Prasetyastuti, Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 26, No 1 (2010)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.478 KB) | DOI: 10.22146/bkm.3479

Abstract

Background: Estrogen has an important role in bone metabolism. The hypoestrogenemia condition occurring in postmenopausal women may affect bone metabolism. The mechanism action of estrogen to prevent the activity of osteoclast in bone resorption was mediated especially by estrogen receptor alpha gene (ESR1). The PvuII polymorphism that occurs in ESR1 gene may alter their expression and  function.Objectives: the aim of this research was to determine the difference of serum calcium and phosphate level in postmenopausal women with PvuII polymorphism of the Estrogen receptor alpha geneMethod: This was cross sectional design. The subjects are postmenopausal women 50-70 year old .Twenty seven subject divided into two group, group I consisted of 15 women with genotype PP,Pp and group II consisted of 12 with genotype pp. polymorphism were assessed by Polymerase Chain Reaction Restriction Fragmen Length Polymorphism(PCR-RFLP) technique. Determination of serum calcium and phosphate level used spectrophotometer. The data was analyzed with Independent sample t-test.Results: The serum calcium level are 8,15 ± 1,35 mg/dl and 8,13 ± 1,58 mg/dl in genotype PP,Pp and pp respectively. The serum phosphate level are 3,8 ± 0,47 mg/dl and 4,24 ± 0,86 mg/dl in genotype PP,Pp and pp respectivelyConclusion: there were no statistically significant differences in serum calcium and phosphate level among genotype PP,Pp with pp  ESRI gene(p>0,05) PvuII polymorphism of ESRI gene did not influence serum calcium and phosphate level in postmenopausal women.Keywords: postmenopausal women, estrogen receptor polymorphism, serum calcium, serum phosphate
Korelasi Antara Kadar Vitamin C dengan Kadar Methemoglobin Darah Lansia di Kecamatan Pakem Kabupaten Sleman Prasetyastuti, Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.427 KB) | DOI: 10.22146/bkm.3572

Abstract

Background: Oxidative damage  by  free  radical can  cause  aging  process. Erythrocyte  as an  oxygen ?  carrying agent  has high  risk  of  oxidative  injury which can  inducemethemoglobin  production.  Vitamin C  is  an  exogen antioxidant  that work  in  aqueous  phase  like  blood  and  can  react   with    free  radical before  they  initiate  the oxidative  injury.Objectives: The  aim of  this study was  to  find out whether  there  is possibility  of correlation  between  vitamin C and methemoglobin  level  in blood of elderly  living  in Pakem DistrictMethod:  This was  an observational  research with  cross  sectional  design,  in which  32  elderly  from  Pakem District were  subjects.  The  blood  sample was  taken  from  cubital  vein.  Determination  of   vitamin C  and methemoglobin  level  used  spectrophotometer. Pearson Correlation was  employed  to  analyze  the  data.Results: The vitamin C and methemoglobin level of the elderly 0,43 + 0,25 mg/dl  and  0,035 +  0.02% respectively. The  correlation between  vitamin C  and methemoglobin  level was  not significant    (  p =  0.771).Conclusion: The  vitamin C was not  correlated with  the methemoglobin  level.Keywords: vitamin C, methemoglobin,  elderly,  free  radica
Hubungan timbal dan krom pada pemakaian pewarna batik dengan kadar hemoglobin dan packed cell volume pada pengrajin batik di Kecamatan Lendah Kulon Progo Hastuti, Pramudji; Sunarti, Sunarti; Prasetyastuti, Prasetyastuti; Ngadikun, Ngadikun; Tasmini, Tasmini; Rubi, Dianandha Septiana; Sutarni, Sri; Harahap, Indrasari Kusuma; Dananjoyo, Kusumo; Suhartini, Suhartini; Pidada, Ida Bagus Gd. Surya Putra; Widagdo, Hendro; Suciningtyas, Martiana
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.051 KB) | DOI: 10.22146/jcoemph.39156

Abstract

ABSTRAK Cara membatik di Indonesia mulai banyak beralih dari pemakaian bahan alami menjadi bahan cat sintesis. Cat sintetis yang digunakan sering kali mengandung logam berat di antaranya timbal (Pb) dan kromium (Cr). Pemakaian logam berat dalam waktu lama akan mengganggu kesehatan. Penelitian ini dilakukan untuk melihat hubungan kadar logam Pb dan Cr dalam darah dan limbah dengan kadar hemoglobin (Hb) dan packed cell volume (PCV) serta mengetahui prevalensi gejala toksisitas yang dirasakan pada pekerja di tiga sentra batik di Kecamatan Lendah Kulon Progo. Sebanyak 76 pegawai di tiga sentra batik terdiri atas 37 orang dari sentra SB, 20 orang dari FA dan 19 orang dari SA ditentukan kadar Hb, PCV, kadar Pb dan Cr-nya dengan metode inductively coupled plasma (ICP). Air limbah lingkungan dari ketiga lokasi sentra batik diambil dan diperiksa kadar Pb dan Cr-nya. Hasil antara ketiga lokasi diujinormalitasnya dan dianalisis dengan analisis varian atau Kruskal-Wallis. Hubungan antara kadar Pb dan Cr baik darah maupun limbah dengan kadar logam berat dalam darah dianalisis dengan uji korelasi Pearson atau Spearman. Hasil dinyatakan berbeda bermakna jika p < 0,05. Gejala penyakit yang dialami pekerja yang diperkirakan berhubungan dengan toksisitas logam berat dihitung frekuensinya. Hasil pemeriksaan antropometri, tekanan darah, kadar Hb, PCV dan logam berat dari ketiga lokasi yang diperiksa tidak berbeda bermakna (p > 0,05). Kadar Pb darah dan krom di sentra SB dan FA berbeda bermakna dengan di sentra SA (p < 0,05). Tidak terdapat korelasi yang signifikan secara statistik antara kadar Pb darah, Cr darah, dan Pb limbah dengan kadar Hb dan PCV. Gejala iritasi kulit, pusing, dan nyeri sendi dirasakan oleh 36,8-48,7% responden. Kadar timbal dan krom darah pembatik di sentra SB dan FA lebih tinggi dari pada di sentra SA. Tidak terdapat hubungan bermakna antara toksisitas Pb dan Cr dengan kadar Hb dan PCV. Gejala toksisitas di antara pekerja batik diperkirakan dapat terjadi karena toksisitas logam berat kronis akibat pemakaian cat batik. KATA KUNCI batik; hemoglobin; kromium; sel darah; timbal ABSTRACT The dye used to color batik has changed from natural to synthetic materials in several places in Indonesia. Synthetic paints often contain heavy metals including lead (Pb) and chromium (Cr). Chronic use of heavy metals can affect health. This study was conducted to examine the relationship between blood and waste water levels of Pb and Cr with hemoglobin (Hb) and packed cell volume (PCV), and to determine how many workers felt the toxicity symptoms in three batik centers in Lendah, Kulon Progo. A total of 76 batik center employees, consisted of 37, 20, and 19 people from the SB, FA, and SA centers were recruited. Determination of Hb, PCV, Pb and Cr levels were conducted with the inductively coupled plasma (ICP) method. Waste water from the three batik centers is taken and examined. The data were analyzed by Oneway ANOVA or Kruskal-Wallis. Correlation analyses were performed with Pearson or Spearman correlation test. Results were considered significant if p < 0.05. Disease symptoms experienced by workers that were estimated to be associated with heavy metal toxicity were calculated in frequency. Anthropometrics, blood pressure, Hb levels, PCV and heavy metals levels were not significantly different between centers (p>0.05). Blood Pb and Cr from the SB and FA were significantly different from those at the SA center (p<0.05). There were no statistically significant correlations between blood Pb levels, blood Cr, and Pb waste with hemoglobin and PCV levels. Symptoms of skin irritation, dizziness, and joint pain were felt by 37-49% of respondents. Blood levels of Pb and Cr in the SB and FA centers are higher than in the SA centers. There was no significant correlation between Pb and Cr toxicity with hemoglobin and PCV levels. Symptoms of toxicity among batik workers are thought to occur due to chronic heavy metal toxicity due to the use of synthetic paint.KEYWORDS batik; haemoglobin; chromium; blood cell; plumbum
Kadar glukosa darah dan tekanan darah pada penduduk pedesaan dan perkotaan di Daerah Istimewa Yogyakarta Tasmini, Tasmini; Farmawati, Arta; Sunarti, Sunarti; Hastuti, Pramudji; Sadewa, Ahmad Hamim; Prasetyastuti, Prasetyastuti; Ngadikun, Ngadikun
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1152.927 KB) | DOI: 10.22146/jcoemph.39569

Abstract

ABSTRAK Sebagian besar penduduk di daerah pedesaan bertaraf ekonomi menengah ke bawah, memiliki keterbatasan akses informasi, dan memiliki mata pencaharian berbeda dibanding penduduk kota. Bantar Kulon merupakan daerah pedesaan dan Kronggahan adalah daerah perkotaan di Yogyakarta. Mengingat terjadinya pergeseran pola penyakit dari penyakit menular ke penyakit tidak menular serta adanya pengaruh lingkungan dan gaya hidup terhadap terjadinya penyakit degeneratif, dilakukan pengkajian mengenai faktor risiko sindroma metabolik di dua daerah tersebut. Penelitian ini dilakukan untuk mengetahui apakah ada perbedaan kadar glukosa darah puasa (GDP) dan tekanan darah (TD), serta keluhan/ penyakit utama pada penduduk di daerah pedesaan dan perkotaan. Sebanyak 71 orang dari Bantar Kulon dan 91 orang dari Kronggahan diperiksa kadar GDP-nya menggunakan GCU Multi-Function Monitoring System (EasyTouch®). Tekanan darah diperiksa dengan sphygmomanometer raksa dan otomatis. Kadar GDP dan TD pada subjek dari kedua lokasi ditampilkan dalam bentuk deskriptif berdasarkan cut-off (GDP: ? 100 mg/dL; TD: ?140/90 mmHg). Uji t atau Mann Whitney U dilakukan untuk mengetahui perbedaaan nilai variabel antara kedua lokasi. Hasil dinyatakan berbeda bermakna jika p < 0,05. Data keluhan penyakit utama ditampilkan secara deskriptif. Tidak ada perbedaan kadar GDP antara desa dan kota (p = 0,385). Persentase subjek yang memiliki GDP ? 100 mg/dL lebih banyak di desa dibanding di kota (42,3% vs 26,4%). Persentase hipertensi lebih tinggi di kota dibanding di desa (50,5% vs 33,8%). Berdasarkan wawancara, keluhan/ penyakit utama terbanyak pada kedua wilayah adalah hipertensi sebanyak 23 orang (32,4%) di desa dan 30 orang (33,0%) di kota. Kadar GDP di atas normal lebih banyak ditemukan di desa sedangkan hipertensi lebih banyak ditemukan di kota. Keluhan/ penyakit utama di kedua wilayah adalah hipertensi. KATA KUNCI kadar glukosa darah; penyakit metabolik; hipertensi; pedesaan; perkotaanABSTRACT Most people living in rural areas are from lower to middle income class, have limited access to information, and have different occupations compared to those in urban areas. In Yogyakarta, Bantar Kulon is a rural area, while Kronggahan is an urban area. Currently, the pattern of disease is shifting from infectious diseases to non-communicable diseases with environment and lifestyle factors as determinants. Thus, it is necessary to study the trends of risk factors for metabolic syndrome in both areas. This study aimed to seek the difference of fasting blood glucose (FBG), blood pressure (BP), and major complaints/illness between rural and urban areas. Seventy one people from Bantar Kulon and 91 people from Kronggahan were examined for FBG levels using GCU Multi-Function Monitoring System (EasyTouch®). Blood pressures were checked using sphygmomanometer. Levels of FBG and BP were presented as frequencies based on cut-offs (FBG: ? 100mg/dL; BP: ? 140/90 mmHg). T-test or Mann-Whitney U test were used to analyze the difference of variables between both areas. Results were significantly different if p < 0.05. Chief complaint ilness data were displayed descriptively. There was no difference in FBG level between rural and urban areas (p = 0.385). The percentage of subjects with FBG ? 100 mg / dL was higher in Bantar Kulon than inKronggahan (42.3% vs 26.4%). Percentage of hypertension was higher in urban than rural areas (50.5% vs. 33.8%). Based on interviews, the chief complaint/ illness in both areas was hypertension. The number of subjects who were diagnosed with hypertension were 23 (32.4%) and 30 (33.0%) from Bantar Kulon and Kronggahan respectively. Impaired fasting glucose was more common in rural area while hypertension is more common in urban area. The chief complaint /illness in both regions is hypertension.KEYWORDS blood glucose; metabolic syndrome; hypertension; rural area; urban area