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DAYA LARVASIDA EKSTRAK DAUN MUDA KEDONDONG HUTAN TERHADAP HAEMONCHUS CONTORTUS SECARA IN-VITRO (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) Wirawan, I Gusti Komang Oka; Nurcahyo, Wisnu; Prastowo, Joko; Kurniasih, Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.2.283

Abstract

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).  
STRONGYLOIDES SPP DISTRIBUTION ON ORANGUTANS IN TANJUNG PUTTING NATIONAL PARK, CARE CENTER IN PANGKALANBUN, AND SEBANGAU NATIONAL PARK Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Strongyloides spp is a parasitic nematode in livestock, primate and human which is  considered asa danger zoonotic disease. Therefore, study about parasite distribution is very important in order to find outgenetic diversity among orangutan in quarantine, zoo and nature, as an effort to explore infection patternand life cycle of Strongyloides spp on orangutan. Amount of 326 orangutan feces were taken from threedifferent habitat of orangutan in Central Borneo, Tanjung Puting National Park, Orangutan Care Centerand Sebangau National Park. Samples which were collected from Tanjung Puting, Care Center and Sebangauwere 75, 80 and 171 respectively. Those samples were transported to the Parasitology laboratory in Facultyof Veterinary Medicine, Gadjah Mada University, Yogyakarta for examination and detection.  Prevalence ofstrongyloides in Tanjung Putting, Sebangau and Orangutan Care Center were 24%, 14,6% and 13,3%respectively. Among positive samples of Strongyloides, 62,5% were from male orangutans, while 37,5% werefrom female orangutans. Strongyloides in pre adult and baby orangutan were 91,6% and 4,2% respectively.Meanwhile, Strongyloides in adult orangutan were very rare. Orangutan habitat in Sebangau National Parkis an ideal habitat for orangutan, supported by the watery condition of peat land, so that Strongyloides re-infection become difficult. Some factors may have important role in Strongyloidoses, such as behavior,physical condition, nutrition, age, body weight, sex, immunity and social status of orangutan.
FAKTOR RISIKO INFEKSI TOXOPLASMA GONDII PADA KUCING DOMESTIK YANG DIPELIHARA DI YOGYAKARTA Hanafiah, Muhammad; Nurcahyo, Wisnu; Prastowo, Joko; Hartati, Sri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2792

Abstract

Tujuan penelitian adalah menentukan kemungkinan faktor-faktor risiko terhadap kemunculan toksoplasmosis. Sebanyak 132 sampel serum darah diambil pada kucing lokal di Yogyakarta. Data-data epidemiologis seperti asal usul kucing, ras kucing, jenis kelamin, umur, lokasi sistem pemeliharaan, jenis pakan, dan frekuensi diare diberi kode untuk mempermudah analisis, kemudian dimasukkan, disimpan, dan dianalisis dengan program Statistix Versi 7 (Analytical Sofware inc). Analisis data dilakukan secara bivariat (Chi-square (?2), dan kekuatan asosiasi (OR), dan multivariat (regresi logistik). Hasil penelitian menunjukkan bahwa faktor-faktor yang berpengaruh terhadap adanya toksoplasmosis pada kucing berdasarkan analisis bivariat adalah pembersihan kotak pasir 1 kali sehari dan mandi 2-3 kali seminggu sedangkan faktor-faktor yang memiliki peluang meningkatkan seropositif toksoplasmosis berdasarkan analisis multivariat adalah pemeliharaan kucing yang bebas di dalam rumah, dimandikan lebih dari 1 kali seminggu, dan dimandikan lebih besar dari 1 bulan sekali.
IN VITRO DEVELOPMENT OF ASCARIDIA GALLI EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Mubarokah, Wida Wahidah; Kurniasih, Kurniasih; Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12978

Abstract

The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
IDENTIFIKASI TOKSOPLASMOSIS PADA FESES KUCING SECARA MIKROSKOPIS DAN SEROLOGIS Nurcahyo, Wisnu; Prastowo, Joko; P, Priyowidodo
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2639

Abstract

Penelitian ini bertujuan mengetahui tingkat prevalensi Toxoplasma pada kucing dan menyusun basis epidemiologis pada kucing yang sangat penting dalam peranannya sebagai hospes definitif toksoplasmosis. Sampel serum darah dan feses kucing digunakan dalam penelitian ini. Metode pemeriksaan menggunakan card agglutination Toxoplasma test (CATT) Pastorex? Toxo kit dan metode sentrifus. Data yang dip eroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa tingkat prevalensi Toxoplasma dengan pemeriksaan serum darah kucing sebesar 6,8% dan pemeriksaan feses sebesar 9,4%. Infeksi toksoplasmosis yang terjadi pada kucing secara umum dari pemeriksaan klinis tidak mempunyai gejala yang spesifik.
PARASIT GASTROINTESTINAL PADA SAPI DI DAERAH ALIRAN SUNGAI PROGO YOGYAKARTA (THE GASTROINTESTINAL PARASITES COWS ON PROGO WATERSHED IN YOGYAKARTA) Nugraheni, Yudhi Ratna; Priyowidodo, Dwi; Prastowo, Joko; Rohayati, Eryl Sri; Sahara, Ana; Awaludin, Aan
Jurnal Ilmu Peternakan Terapan Vol 1, No 2 (2018)
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipter.v1i2.889

Abstract

INTISARIPenelitian ini bertujuan untuk mengidentifikasi parasit gastrointestinal pada sapi yang berlokasi di daerah aliran Sungai Progo. Penelitian ini menggunakan feses sapi sebanyak 63 sampel yang diambil dari daerah aliran Sungai Progo. Pemeriksaan feses menggunakan metode sentrifuse, dan Parfitt Bank. Hasil penelitian menunjukkan bahwa  63 sampel feses sapi yang diperiksa, 25 diantaranya terinfeksi cacing jenis Fasciola sp., 7 ekor Paramphistomum sp., 14 Strongyle, dan 9 Coccidiosis.  Kesimpulan dari penelitian ini adalah prevalensi parasit gastrointestinal pada sapi di aliran Sungai Progo yang dominan adalah kejadian Fasciolosis sebesar 40%, infestasi nematodiasis jenis Strongyle 22%, Coccidiosis 14%, dan infestasi Paramphistomum sp.11%. Kata kunci: Coccidiosis, Fasciola sp., Parampistomum sp., Strongyle   ABSTRACTThe aim of study was investigate gastrointestinal disease on cow fed forage from Progo watershed. Sixty three feses samples were taken from cows on Progo watershed. The method of feses analysis used was sentrifuge and Parfitt Bank. The results showed that 63 samples of cows feces were examined, among 25 were infected by Fasciola sp., 7 Paramphistomum sp., 14 Strongyle, and 9 Coccidiosis. The conclusion was the prevalence of gastrointestinal disease in cows was dominant incidence of Fasciolosis by 40%, Strongyle 22%, 14% Coccidiosis, and Paramphistomum sp.11%. Keywords: Coccidina, Fasciola sp., Parampistomum sp., Strongyle
THE EFFECT OF APUS BAMBOO (GIGANTOCHLOA APUS) LEAVES INFUSION TO MORTALITY RATE AND MORPHOMETRY OF HAEMONCHUS CONTORTUS ADULT WORM IN VITRO Widiarso, Budi Purwo; Nurcahyo, Wisnu; Kurniasih, Kurniasih; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8167

Abstract

This present project was carried out to determine the effect of apus bamboo (Gigantochloaapus )leaves infusion to mortality rate and morphometry of Haemonchus contortus adult worms in vitro. The formulated tools were collected through camera, 3 ml syringe, object glass, deck glass, mortar, drip dropper, waterbath, microscope, stopwatch, counter check, oven, erlenmeyer flask, electric scale, surgical scissor, and pipette. The allocated materials used in this studywere bamboo leaves infusion, Haemonchus contortus adult worms, aquadestilata, ethanol and NaCl 0.62%. The research was firstly concernedwith making 0.1% and 1% apus bamboo leaves infusion. The samples were obtained from Haemonchus contortus collection. Adult worms were obtained directly from abomasum of naturally infected goats that were cut at Animal Slaughter House (RPH). To investigate the matter, mortality rates of adult worms at various doses and times of observation as well as differences in Haemonchus contortus morphometry were analyzed using ANOVA. The findings disclosed that apus bamboo leaves infusion demonstrated a significant difference in the mortality rate of adult worms Haemonchus contortusat various doses and times of observation. This report presented the findings of research that the best dose to increase the mortality of worms was 1% bamboo leaves infusion. Moreover, apus bamboo leaves infusion affected the morphometry of Haemonchus contortus adult worms, especially in body length, cervical papilla width, and spicules length in males, and body length, cervical papilla width, and vulvar length in females.   
THE EFFECT OF INCUBATION TIME ON EXCYSTATION OF EIMERIA TENELLA SPOROZOITES IN VITRO Prastowo, Joko; Mufasirin, Mufasirin; Sumartono, Sumartono
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8596

Abstract

This purpose of the research is to know the effect of incubation time on excystation of Eimeria tenella sporo-zoites in vitro.Eimeria tenella oocystes treated in clorox were broken by glass beads. Sporocystes liberated from oocys were divid­ed in six groups (I - VI), each ghroup contained 1.68.106 sporocystes. All of the groups were treated by 0,75% taur-ocholat sodium and 0,25% trypsin. Those sporocystes were incubated at 41°C for 15, 30, 45, 60, 75 and 90 minutes respectively. The data collected was the percentage at the number of sporozoites found in each group.The result showed that the increase of the number of sporozoites was paralelly with the incubation time. The highest number of sporozoites excystation was found in the 90 minutes of incubation group.
DYNAMICS OF NUMBER OF NEMATODE LARVAE IN GRASS IN THE PASTURE Prastowo, Joko; Sumartono, Sumartono
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8641

Abstract

The existence of nematode larvae in a pasture can be used as an indication of infection or reinfection in animal grazing on it. The objective of the current study was to reveal the dynamics of the number of nematode larvae found on the grass of a pasture.Three locations of the pasture of the teaching farm of Faculty of Veterinary Medicine GMU, were chosen as sampling areas. Grass of the areas was cut just on top of the soO five times a day for ten day namely at 5, 6-9 am - 1 pm, 2-5 pm and 6 pm. Following cutting, the grass was kef t m a tube and washed with detergent solution. The washing solution was examined under a stereomicroscope, and the larvae observed were counted. Differences in the number of larvae between locations and collection times were analysed statistically by names of factorial analysis of variance.The results show that the mean number of larvae varies between 03 ± 0.67 and 6.1 ± 2.95 per grass. The number of larvae is not significantly different (F > 0,05). Cutting at however, is influenced by collection time (P < 0,05). Cutting at 5-9 am gives the largest number of larvae, while 6 pm gives the lowest count.  '
INFEKSI NEMATODA GASTROINTESTINAL PADA ORANGUTAN (PONGO PYGMAEUS)DI ICEBUN BINATANG GEMBIRA LOKA YOGYAKARTA Rianawati, .; Prastowo, Joko
Jurnal Sain Veteriner Vol 21, No 1 (2003): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1187.19 KB) | DOI: 10.22146/jsv.491

Abstract

Telah dilakukan penelitian tentang infeksi nematoda gastrointestinal pada orangutan (Pongo pygmaeus) di Kebun Binatang Gembira Loka Yogyakarta. Penelitian ini bertujuan untuk mengetahui garnbaran infeksi nematoda gastrointestinal dengan melakukan inventarisasi cacing tersebut di Kebun Binatang Gembira Loka Yogyakarta. Penelitian ini dilakukan dengan mengambil sampel tinja dan 13 ekor orangutan seminggu sekali selama 8 minggu dan diperiksä dengan metode natif, sentrifus dan Mc. Master. Telur cacing diidentifikasi berdasar bentuk dan ukuran, serta dihitung jumlahnya setiap gram tinja. Gejala klinis yang diarnati adalah kondisi tubuh seperti berat badan, aktivitas, nafsu makan dan bentuk tinja. Basil penelitian menunjukkan bahwa semua orangutan di Kebun Binatang Gembira Loka Yogyakarta terinfeksi cacing Strongyloides fitelleborni dan 2 ekor orangutan terinfeksi cacing Trichuris trichiura. Dengan analisis t-test dibuktikan bahwa tidak ada penganih yang nyata antara jenis kelamin dan tipe kandang dengan kejadian nematodiasis pada orangutan. Infeksi yang ditimbulkan oleh cacing Strongyloides fuellehorni cukup berat hingga menimbulkan gejala klinis diare, nafsu makan turun, lemah dan lesu, sedangkan infeksi oleh Trichuris trichiura tergolong ringan. Kata kunci : nematoda gastrointestinal, orangutan (Pongo pygmaeus), gembira loka