Hery Pratiknyo
Fakultas Biologi, Universitas Jenderal Soedirman Purwokerto

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A Life Table of Spodoptera litura Fabr. with Different Artificial Diets Lestari, Sri; Ambarningrum, Trisnowati Budi; Pratiknyo, Hery
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.06 KB) | DOI: 10.22146/jsv.3801

Abstract

Abstract Sustainable insect supply is a routine activity in research of pest control, experimental insecticide bioassay, entomophatogen, parasitoid, and nature of competitor. Armyworm (Spodoptera litura Fabr.) is one of the alternative test insect. A mass rearing method is required to provide high demands of S. litura. A life table of insect is regularly used to study several biological parameters including the net reproduction rate of population (Ro), the mean length of life period in one generation (T), reproduction potential value of one generation from a population (rm), and the potential of a population to multiply in one generation (ë). The aim of this study were to asses the development time and reproduction potential of S. litura which different of artificial diets. Results showed that S. litura on artificial diet in recipe of Singh and Moore is T = 29,90, R = 2692,33, r = 0,26, ë = 1,29, o m whereas artificial diet in recipe of Waldbauer et al. is 32,29, R = 891,99, r = 0,21, ë = 1,23. o m
Populasi Tungau Parasit Varroa jacobsoni: Penyebab Kematian Pupa dan Korelasinya terhadap Produksi Madu Apis cerana pada Sisiran Sarang Membujur dan Melintang Pratiknyo, Hery; Darsono, Darsono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.248

Abstract

Bee keeping of local bee (Apis cerana Fab.) in Purbalingga Regency is spread widely from  25 m to 350 m asl, eventhough quatatively the production is still low. It is caused by parasitic mite attack,  Varroa jacobsoni. This mite attack then suck haemolymph of the pupae,  meanwhile if the bee comb cell  consist of more than one mites, the pupae will die, consequently the honey production will decrease. The aims of the research are: 1) to know a mount of individual mite living and caused the death of one bee pupae, .2) to define the correlationship between density of parasitic mite and honey production, 3). To differ parasite mite V. jacobsoni  density in longitudinal and cross posision of combs.  Method used was survey with purposive random sampling tehnique on two posision of bee comb, 15 samples were taken in each posision.  The data were analyzed  by counting the amount of mite living on every dead pupae and the correlation was analyzed for the relationship between relative density of parasite mite and  honey production. The result shows that correlation between relative density of parasitic mite and honey production is unsignificant. Conclusion of the research are: 1) amount of  parasitic mite living on bee pupae and causing death is 5,80 individual on cross posisition comb and 4,71 individual on longitudinal posision comb. 2) correlation between relative density of parasitic mite and honey production is unsignificant. 3). The relative density of parasitic mite between two posisions of comb is unsignificant.
Kajian Status Resistensi Tungau Hama Brevipapalpus phoenicis dan Tungau Predator Ambleyseius deleoni sebagai Dasar Evaluasi Aplikasi Pestisida Pratiknyo, Hery; Basuki, Edi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.115

Abstract

The Increasing demand of tea product this decade causes the tea become prospective commodity. In other hand, free residual of pesticide in the tea culture could inhibit the rise of this demand. Therefore the evaluation of pesticide application policy in the field should be conducted, especially in term of the effect of the residual of pesticide on the resistance status of the mite. The research had been done using survey and experimental methods. The aim of the research was to decide the resistance status of the pest mite of tea leaf Brevipalpus phoenicis and it main predatory mite Ambleyseius deleoni on the application of three kind of pesticides (herbicide, fungicide and acaricide), and its effect on predator-prey feeding link. The result shows that both B. Phoenicis and A. deleoni are still resistant to herbicide (Round Up 486 AS) and fungicide (Kocide 77WP), but they are susceptible on acaricide (Kelthane 200EC). Also pesticide application has no effect significantly on predator-prey link (predation level). The conclusion is pest mite B. phoenicis is resistant on fungicide (Kocide 77WP) and Herbicide (Round Up 486 AS) but it is not resistance on Acaricide (Kelthane 200EC). Application of pesticides has no effect significantly on predator-prey link (predation level), so predatory mite A. deleoni is still effective in biological control for its prey.
HUBUNGAN ANTARA KERAGAMAN TUNGAU FAMILI Macrochelidae (ACARI: MESOSTIGMATA) DAN FAKTOR LINGKUNGAN PADA MANUR AYAM PEDAGING DENGAN TIPE KANDANG PEMELIHARAAN BERBEDA Firmansyah, Nurhadi Eko; Budianto, Bambang Heru; Pratiknyo, Hery
Scripta Biologica Articles in Press
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Livestock of broiler chickens using several types of cages, including the type of litter stage and litter postal. Treatment of chicken manure in both types of cages will cause the condition of the manure as the habitat of mite family Macrochelidae will be different. The diversity of mites family Macrochelidae are suspected to be different in manure found on stage litter cages compared to litter postal cages and influenced by some environmental factors. The aim of this study is to analyze the diversity of mites family Macrochelidae and its relation to environmental factors. The obtained manure sample was then taken to the laboratory and placed on a modified Barlese-Tullgren for mite isolation. The mites obtained are then counted, preserved, and identified at the Laboratory. Environmental factors measured are temperature and humidity. The diversity data obtained were analyzed using the Shannon-Wiener index and the Shannon-Evennes index. Pearson correlation test to determine the relationship between temperature and humidity to the diversity of mites of Macrochelidae family. Five species of mite Macrochelidae are Macrocheles sp.1, Macrocheles sp.2, Glyptholaspis sp., Neopodocinum sp., Holostaspella sp. The diversity index of mites family Macrochelidae on manure litter stage is 0.81354. The index diversity of mites on manure litter postal is 0.79871. The humidity factor influences the diversity of the mites of family Macrochelidae in both types of cages.
KEHADIRAN RAYAP Schedorhinotermes javanicus (F: RHINOTERMITIDAE) PADA TONGGAK POHON JATI (Tectona grandis) DAN WANGKAL (Albizia procera) DI CAGAR ALAM BANTARBOLANG PEMALANG JAWA TENGAH Himawanti, Hena; Widhiono, Imam; Pratiknyo, Hery
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.355 KB) | DOI: 10.20884/1.bioe.2019.1.2.1695

Abstract

Rayap menjadikan kayu sebagai sumber makanan sekaligus sebagai tempat tinggal (shelter). Di hutan, terdapat banyak tonggak kayu sebagai sumber makanan rayap. Rayap akan dihadapkan pada banyak pilihan makanan sehingga rayap akan memilih tipe makanan yang paling disukai.Tujuan penelitian ini yaitu untuk mengetahui kehadiran rayap Schedorhinotermes javanicus (familia Rhinotermitidae) pada Tonggak Pohon Jati (Tectona grandis) dan Wangkal (Albizia procera) di Cagar Alam Bantarbolang Pemalang, Jawa Tengah. Penelitian ini menggunakan metode survey dengan teknik sampling diatur dengan pola Rancangan Acak Kelompok (RAK). Sebagai plot utama adalah kedalaman masuk hutan (0 m, 50 m, 100 m, 150 m dan 200 m) dari tepi hutan, sedangkan ulangan sekaligus blok berupa kelompok umur tonggak sejak pemotongan. Spesimen rayap diambil pada tonggak kayu Jati (Tectona grandis) dan Wangkal (Albizia procera) yang terdapat pada area 0 m sampai 200 m dari tepi hutan. Dengan memperhatikan umur tonggak sejak pemotongan pohon. Hasil penelitian menunjukkan tidak terdapat pengaruh yang signifikan dari umur tonggak dan jarak tonggak dari batas tepi hutan dengan kehadiran rayap S. javanicus pada tonggak kayu Jati (Tectona grandis) dan Wangkal (Albizia procera).
Preferensi Rayap (Isoptera: Termitidae) Pada Berbagai Tonggak di Kawasan Cagar Alam Bantarbolang Pemalang Jawa tengah Deviriani, Risty; Widhiono, Imam; Pratiknyo, Hery
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.06 KB) | DOI: 10.20884/1.bioe.2019.1.2.1800

Abstract

Termite family Termitidae is a type of wood-eating termites, humus, or materials consisting of cellulose. This familia termite has a role in helping the ecosystem as a decomposer by destroying wood or other organic material and returning it as nutrients to the soil. Preference or prefer food sources that exist in the environment, influential in supporting the development of termites. The purpose of this study is to find out the type of wood that is a food preference for termites and species of the termite family Termitidae in the Bantarbolang Pemalang Nature Reserve in Central Java. This research was conducted at the Bantarbolang Nature Reserve located in the Kebon Gede Village Area, Bantarbolang District, Pemalang Regency, Central Java. The research method used survey method with termite sampling technique on the milestone paying attention to the depth of entering the forest (0 m, 50 m, 100 m, 150 m and 200 m) from the edge of the forest and paying attention to the age of the post since cutting trees. Termite species were taken on teak (Tectona grandis), Wangkal (Albizia procera), and Mahoni (Switenia marcrophilla) stakes found in areas of 0 m to 200 m. The data obtained were analyzed using analysis of variance (ANOVA). The termites taken are inserted into vial bottles containing 70% alcohol and all termite colonies are counted to determine the number of individual termites. Measurement of environmental parameters include temperature, humidity, soil pH, canopy cover and light intensity. The results obtained by a species of termite Macrotermes gilvus familia Termitidae. The results of data analysis using the f test on teak (Tectona grandis), Wangkal (Albizia procera), and Mahoni (Switenia marcrophilla) milestones show that the significance value is > 0.05, which means the age of the milestone and the distance of the milestone from the forest edge do not affect significantly to the presence of Macrotermes gilvus termites on these wooden posts. The conclusion of this study is that the preferences of Macrotermes gilvus termites for various wooden stakes in the Bantarbolang Nature Reserve are not influenced by the age of the stakes and the distance of the wooden stakes from the edge of the forest.
Efektivitas Zat Aktif Fipronil pada Berbagai Substrat Fagostimulan untuk Pengendalian Kecoak Jerman (Blattella germanica L.) Maula, Riska Isna; Pratiknyo, Hery; Susilo, Untung; Ambarningrum, Trisnowati Budi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.841 KB) | DOI: 10.20884/1.bioe.2020.2.2.1859

Abstract

German cockroach (Blattella germanica L.), is a residential insect pest and vector of various diseases. To control its population, alternative chemical control models with Fipronil 0.03% are now being developed. Fipronil has been proven to be effective and has no resistance. To increase the effectiveness of the bait in controlling German cockroaches, a fipronil 0.03% combination needs to be developed with a phagostimulant bait of bananas, durian, erythisol sugar, and sugar formulation, so this study needs to be done. This study used an experimental method with a randomized block design (RBD), five treatments such as A: bait with a combination of banana phagostimulant and 0.03% fipronil, B: bait with a combination of durian phagostimulant and 0.03% fipronil, C: bait with a combination erythisol phagostimulant and 0.03% fipronil, D: bait with a combination of sugar phagostimulant and 0.03% fipronil, K: bait with a combination of matrix and fipronil 0.03% (control positive) was used for the interest test, and bait with a matrix without fipronil 0.03% (control negative) was used for mortality testing. The observations the average results for durian, banana, sugar formulated, erytrisol sugar and controls, respectively 20%, 20%, 21.6%, 12.4%, and 22.8%, while the results bait consumed with durian stimulation are 0.29g, banana 0.31g, sugar formulation 0.28g, erytrisol sugar 0.26g and control 0.24g. However, based on statistical tests, the results of the interest test with the feed consumption test between treatments showed no significant difference (p>0.05). The test results in mortality of cockroaches Germany's feed with phagostimulan durian, banana, formulations sugar, sugar erytrisol and control consecutively 98% 100 %, 98%, 98%, and 20%, based on the statistical test there is a significant difference (p<0.05), where the difference based on the Duncan test is their treatment compared to control.
The Diversity and Abundance of Termites (Order: Isoptera) at Altitude 200 M ASL Karst Area Southern Gombong, Kebumen Regency nufus, Wafiyyatun; Pratiknyo, Hery; Sukarsa, Sukarsa
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.966 KB) | DOI: 10.20884/1.bioe.2020.2.1.1816

Abstract

Termites are social insects that have a very wide distribution. The termites in the forest ecosystem have many species and feeding habits, it can be said that diversity and abundance determine the role in the termite ecosystem. The termite are very important as a mediator for decomposition of the ecosystem of karst area. Therefeore, the aim of this study was to determine the diversity and abundance of termites in kasrt area at an altitude of 200 m asl Southern Gombong, Kebumen Regency. The study was conducted in a survey method and arranged as follows, in which 20 sub transect size of 5 m x 2 m as repetition and the termite was found is taken to vial bottle with alcohol 70% for identification. The data analysis using F test and continue with the LSD analysis. The study was obtained three diversity of termite in the karst area, there are M. gilvus, M. inspiratus and S. javanicus. The abundance of termite was obtained by M. gilvus is highest than the other termite with percentage of 90% while the abundance of termites based on plot was obtain in plot 1 with percentages 30,4%.
Ketertarikan dan Kesukaan Kecoak Jerman, Blatella germanica L. (Dictyoptera: Blattellidae) Terhadap Fagostimulan Berbeda Ilhami, Femilia Hajar; Ambarningrum, Trisnowati Budi; Pratiknyo, Hery
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.888 KB) | DOI: 10.20884/1.bioe.2020.2.2.1983

Abstract

German cockroaches (Blatella germanica) L. are includes in settlement bugs that are hard to terminate. One alternative to control German cockroaches that are using bait. To make bait formulation, the first thing is look for the type of phagostimulant that can be interesting and attractive to German cockroaches. The purpose of this research is to study the type of phagostimulant that has the ability to attract and liked by German cockroaches. Phagostimulant types used are bananas, durian, sugar formulations, and erythrisol. Data from observations of interest in test interest were analyzed using the t test and calculation of the attraction index, while the data for German roaches favored were analyzed using ANOVA and index feeding calculations. The results obtained were that German cockroaches were attracted to bananas, durian, sugar formulations, and erythrisol. ANOVA analysis results obtained sig 0.029 (p <0.05). The results of the calculation of the attractiveness index and feeding index obtained German cockroaches are preferred by bananas compared to other phagostimulants. The conclusion of this study is the type of phagostimulant that can attract cockroaches Germany is banana, durian, sugar formulation, and erythrisol, while the type of phagostimulant most preferred by German cockroaches is banana.