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PEMANFAATAN MANGROVE RHIZOPHORA MUCRONATA SEBAGAI PEWARNA ALAMI KAIN KATUN Dewi, Lutfianna Fatma; Pringgenies, Delianis; Ridlo, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v7i2.25896

Abstract

ABSTRAK : Bagian-bagian pohon dari R. mucronata seperti serasah daun, kulit kayu, maupun limbah propagul diketahui memiliki kandungan pewarna yang ramah lingkungan. Potensi pewarna alami dari tumbuhan ini dapat menjadi alternatif bahan produksi bagi industri batik di Indonesia. Pengambilan sampel dilakukan di Teluk Awur, Jepara, Jawa Tengah pada bulan Oktober 2015 kemudian dilakukan ekstraksi pewarna, pewarnaan, dan berbagai analisis. Tujuan penelitan adalah untuk mengetahui kualitas pewarna alami dari bagian-bagian pohon R. mucronata sebagai pewarna alami pada kain katun. Sampel R. mucronata yang diambil yaitu kulit kayu, limbah propagul, dan serasah daun. Ekstraksi pewarna dilakukan menggunakan air panas pada suhu 30°C, 50°C, dan 70°C. Pengikatan warna setelah pencelupan menggunakan kain katun mori primissima dengan mordan tawas. Hasil penelitian menunjukkan bahwa warna cokelat dihasilkan oleh ekstrak R. mucronata. Analisis FTIR dan UV Vis mengindikasikan adanya senyawa tanin terkondensasi. Pengujian Total Phenol Content (TPC) dan Total Flavonoid Content (TFC) menunjukkan hasil positif sebesar 2,4950 mg GAE/g untuk TPC dan 0,6516 mg QE/g untuk TFC. Hasil pindaian dengan Scanning Electron Microscope menunjukkan granula-granula yang pecah pada spesimen. Kualitas pewarnaan pada kain menunjukkan kisaran hasil antara 3 (cukup) hingga 4 (baik) dan telah memenuhi standar SNI. Pewarna dari serasah daun pada ekstraksi 70°C menunjukkan hasil terbaik di antara yang lain. ABSTRACT : The tree parts of R. mucronata such as the leaves litter, bark, and propagule are known for containing dyes that are environmentally friendly. This natural dye potential could be used for the production resource alternative for the batik industry in Indonesia. Sampling was conducted in Teluk Awur, Jepara, Central Java on October 2015 and followed by dye extraction, dying process, and also several analysis. The purpose of this research was to discover the quality of natural dye from R. mucronata on cotton fabric. Samples that were taken from R. mucronata were bark, leaves litter, and propagule litter. Dye extraction used hot water method with variants of temperature: 30°C, 50°C, dan 70°C. The color locking after dyeing used cotton fabric with mori primissima type and alum as the mordant. The obtained result from extract R. mucronata showed brown color. Condensed tannin was indicated from FTIR and UV Vis analysis. Total Phenol Content and Total Flavonoid Content assays showed positive result as follows: 2,4950 mg GAE/g for TPC and 0,6516 mg QE/g for TFC. Scanning result using Scanning Electron Microscope showed that the granules break on the specimen. Color fastness quality showed the range of result from colored fabric from 3 (enough) until 4 (well) and already fulfilled SNI standard. The dye extracted from leaves litter in 70°C showed the best result among the others. 
KAJIAN AKTIVITAS BIOAKTIF EKSTRAK TERIPANG PASIR (HOLOTHURIA SCABRA) TERHADAP JAMUR CANDIDA ALBICANS Pranoto, Eunike Noviana; Ma'ruf, Widodo Farid; Pringgenies, Delianis
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 1, No 2 (2012): Jurnal Pengolahan dan bioteknologi hasil perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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Abstract

Teripang dapat ditemukan di perairan Indonesia. Teripang diketahui memiliki zat yangsalah satunya berfungsi sebagai antijamur. Penyakit infeksi pada manusia yangdisebabkan oleh jamur Candida albicans di Indonesia masih relatif tinggi dan obatantijamur relatif lebih sedikit dibandingkan dengan antibakteri. Pengobatan terhadap C.albicans secara kimia dapat menimbulkan resistensi dan efek samping. Penelitian inibertujuan untuk mengetahui pengaruh penggunaan pelarut dan konsentrasi yang berbedadari ekstrak Holothuria scabra terhadap C. albicans serta mengetahui kandungansenyawa bioaktif dalam H. scabra. Hasil penelitian didapatkan bahwa pelarut terbaikuntuk mengisolasi senyawa antijamur H. scabra adalah metanol. Konsentrasi ekstrak 5mg/ml, 6 mg/ml,7 mg/ml menghasilkan zona hambat berturut-turut sebesar 5,1 + 0,56mm; 8,42 + 0,71 mm; 10,1 + 0,59 mm. Hasil uji kandungan bioaktif memperlihatkanbahwa ekstrak H. scabra mengandung senyawa alkaloid, saponin, steroid dantriterpenoid. Ada indikasi potensi antijamur pada ekstrak H. scabra.
ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER STICHOPUS VASTUS EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA Pringgenies, Delianis; Ridlo, Ali; Sembiring, Nerva
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v9i2.19302

Abstract

The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The study revealed that from 5 species MDR bacteria, which are Coagulant negative stapylococi (CNS), E. coli, Enterobacter 5, Klebsiella sp. dan Pseudomonas sp. There are two MDR bacteria had the most sensitive responses by the extract of sea cucumber, which were Enterobacter-5 and Klebsiella sp. The two bacteria were tested against five bioactive fractions obtained from OCC. Fraction criteria-2 had the highest antibacterial activity against Enterobacter-5 and Klebsiella sp, with serial concentration of 20 µg ? disc?1, 40 µg. disc?1 and 80 µg. disc?1.  Largest inhibition zone were obtained from 80 µg. disc?1 againts the two bacteria were (14.73 ± 0.48) mm and (11.22 ± 0.85) mm respectively.  GC-MS Analysis revealed that fraction criteria-2 had (or consisted of) cyclohexhane, ethanol, butanoic and pentanoic acids. Keywords: antibacterial activity, multi drug resistance (MDR), sea cucumber, Stichopus vastus
KARAKTERISASI METABOLIT SEKUNDER BAKTERI SIMBION GASTROPODA CONUS MILES DENGAN METODE GC-MS SEBAGAI ANTIBAKTERI MDR (MULTI DRUG RESISTANT) Hasanah, Nurul Fitrah; Pringgenies, Delianis; Wulandari, Sri Yulina
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v1i2.2038

Abstract

It is known that the bacterial symbionts are symbiotic with gastropods Conus miles has the potential to be able to produce antibacterial compounds against bacterial MDR (Multi Drug Resistant). Information found previously from bacterial symbionts and isolation based on screening results, the size of the zone of inhibition and inhibitory properties against several types of bacteria tests showed that isolates derived from Conus miles Gastropoda symbionts characterized by kinship rate of 98% in bacteria Pseudoalteromonas sp. The research objective was to determine the characteristics of the bacterial symbiont bioactive compounds associated with the type of mollusk Conus miles. The study was conducted three phases namely the isolation and purification of bacteria; characterization of isolates identified through test results on the reaction of physiological and biochemical tests, as well as the identification of secondary metabolites by GC-MS method QP2010S Shimadzu. The results showed that the bacterial symbiont isolates had a number of compounds with a percentage of the highest peak to the lowest compound the compound, Acetic acid (CAS) ethylic acid (35.22%); propanoic acid, 2-methyl-(CAS) Isobutyric acid (11.78%); Iso -valeric acid (9:38%), butanoic acid, 2-methyl-(CAS) 2-methylbutanoic acid (4.74%) and 1,2-Benzenedicarboxylic acid, dioctyl ester (CAS) Dioctyl phthala (4:36%). The results concluded that the bacterial symbiont isolates obtained in this study provide information on the results of potentially new antibiotics.
UJI FITOKIMIA DAN TOKSISITAS EKSTRAK KASAR GASTROPODA (TELESCOPIUM TELESCOPIUM) TERHADAP LARVA ARTEMIA SALINA Putri, Mukti K. Diana; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v1i2.2020

Abstract

Gastropod is one of the marine organisms that can be used as a source of natural bioactive compounds, one of which is Telescopium telescopium. T. telescopium until now it has not been yet exploited optimally, some people still use it as feed ingredients that contain high protein nutrition, however it must be supported by scientific information about the efficacy and side effects. This research aims at determining the secondary metabolite by crude extract T. telescopium, and the lethal toxicity value (LC50-24 hours). The research consisted of two steps, namely the phytochemical and toxicity test. The method used exploratif and experimental laboratory with a complete randomized design, which consited of five concentration treatments namely 25.12, 63.1, 158.5, 398.15, and 1000 ppm with three replications. Each treatment used 10 Artemia salina larvae, and observations were made during 24 hours of Artemia salina larvae mortality. LC50 was determined by probit analysis using the EPA Probit Analysis Program Version 1.5 Finney. The results of the phytochemical test showed that crude extract T. telescopium contain alkaloid compounds, steroids, flavonoids. The results BSLT (Brine Shrimp Lethality Test) of crude extract T. telescopium LC50-24 hours showed that the crude extract clorofom (229.562 ppm), crude extract ethyl acetate (244.906 ppm), and crude extract methanol (197.242 ppm). LC50-24 hours < 1000 ppm showed that the crude extract Telescopium telescopium potent antitumor compounds.
KAJIAN AKTIVITAS BIOAKTIF EKSTRAK TERIPANG PASIR (HOLOTHURIA SCABRA) TERHADAP JAMUR CANDIDA ALBICANS Pranoto, Eunike Noviana; Ma'ruf, Widodo Farid; Pringgenies, Delianis
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 1, No 1 (2012): Jurnal Pengolahan dan bioteknologi hasil perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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Abstract

Sea cucumbers are found in Indonesian oceans. Sea cucumbers have been known containing substances as antifungal. Infection diseases in human caused by fungi, such as Candida albicans, are still high and antifungal drugs are less than antibacterial drugs in amount. Medical curing of Candidiasis chemically can use chemical agents. But they can caused resistance and side effects. The purpose of this research is to know the influence of different solvent and concentration usage of Holothuria scabra extract towards C. albicans and to know the bioactive substances contained in H. scabra. The results obtained from this research were: the best solvent to extract antifungal agents from H. scabra is methanol. Extract concentration of 5 mg/ml, 6 mg/ml,7 mg/ml showed inhibition zone continuously as high as 5,1 + 0,56 mm; 8,42 + 0,71 mm; 10,1 + 0,59 mm. The results are significantly different (P < 0,01), different (0,01 < P < 0,05), and not different (P > 0,05) for antifungal activity test. Bioactive substance test showed that alkaloid, saponin, steroid, and triterpene were found in H. scabra. H. scabra extract indicates potential activity as antifungal agent.    
IDENTIFIKASI PIGMEN KAROTENOID PADA BAKTERI SIMBION KARANG POCILLOPORA DAMICORNIS Idris, Ryandha; Riniatsih, Ita; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v3i3.5996

Abstract

Karotenoid merupakan pigmen merah, kuning dan orange yang diketahui memiliki aktivitas antioksidan. Karotenoid dapat ditemukan pada tanaman, hewan dan bakteri.  Penelitian bertujuan untuk mengidentifikasi pigmen karotenoid pada bakteri simbion karang Pocillopora damicornis. Identifikasi pigmen menggunakan Spektrofotometer UV-Vis pada panjang gelombang 190-800 nm dan Kromatografi Cair Kinerja Tinggi (KCKT) fase terbalik ODS/C18 dengan fase gerak metanol:asetonitril (7:3 v/v) pada panjang gelombang 190-800 nm. Uji DPPH dilakukan dengan metode diphenylpicrylhydrazil (DPPH) dan pengukuran absorbansi dilakukan pada panjang gelombang 517 nm. Identifikasi bakteri simbion karang Pocillopora damicornis dilakukan menggunakan metode PCR 16S rDNA. Hasil penelitian memperlihatkan bahwa dari 9 isolat bakteri simbion terdapat 1 isolat bakteri simbion karang Pocilllopora damicornis yang positif memiliki pigmen kaotenoid yaitu 5.A.4.Isolat 5.A.4 mengandung pigmen karotenoid Prasinoxanthin, Alloxanthin, Siphonein dan Crocoxanthin yang merupakan kelompok xantofil dan memiliki aktivitas peredaman radikal bebas DPPH sebesar 6,12%. Hasil identifikasi bakteri dengan metode PCR 16S rDNA menujukkan bahwa isolat bakteri 5.A.4. memiliki tingkat kekerabatan sebesar 99% dengan bakteri Bacillius subtilis.Hasil penelitian menunjukkan bahwa bakteri karang P. damicornis mempunyai potensi untuk dikembangkan sebagai sumber pigmen alami yang berasal dari laut dan berkelanjutan.
BIOPROPEKSI BAKTERI SIMBION DARI GASTROPODA CONUS MILES TERHADAP STRAIN BAKTERI IMDR (MULTI DRUG RESISTANT) Pringgenies, Delianis
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 1 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.741 KB) | DOI: 10.14710/ik.ijms.14.1.42-49

Abstract

Keberadaan bakteri yang berasosiasi dengan moluska laut telah memungkinkan penggunaan mikro-organisme tersebut sebagai sumber senyawa bioaktif yang baru termasuk senyawa antimikroba khususnya dalam menangani strain strain yang resisten terutama multi-drugsresistant (MDR). Penelitian ini bertujuan untuk mengisolasi bakteri dari gastropoda Conus miles, menskrining bakteri simbionnya yang berpotensi sebagai antibakteri terhadap strain bakteri MDR. Sampling moluska dilakukan di perairan pulau Ternate, Maluku. Selanjutnyadilakukan isolasi bakteri, skrining bakteri penghasil senyawa anti-MDR, uji antibakteri moluska, isolasi bakteri patogen klinik (MDR), uji sensitifitas antibakteri dan analisis sekuen 16S rDNA. Hasilpenelitian memperlihatkan bahwa bakteri simbion Conus miles yang dapat menghambat bakteri MDR berjumlah 16 isolat. Sebanyak 9 isolat memiliki kemampuan menghambat beberapa jenis bakteri MDR, sedangkan sebanyak 7 isolat hanya dapat menghambat satu jenis bakteri MDR. Berdasarkan besarnya zona hambatan yang dibentuk dan kemampuan daya hambatan maka dipilih satu isolat terbaik untuk uji lanjutan, yaitu isolat TCM 6.1. Hasil identitlkasi I6S rDNA menunjukkan bahwa isolat TCM 6.1 memiliki kekerabatan terdekat dengan bakteri Pseudoalteromonas sp. dengan tingkat homologi 99 %.   Kata kunci : screening, bakteri simbiotik, Conus miles, antibacterial, multi-drug resistant
UJI FITOKIMIA DAN AKTIVITAS ANTIJAMUR EKSTRAK TERIPANG KELING (HOLOTURIA ATRA) DARI PANTAI BANDENGAN JEPARA TERHADAP JAMUR CANDIDA ALBICANS Septiadi, Tedi; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v2i2.2355

Abstract

Holothuria atra is one of the marine lifes that lives at the bottom of the substrate, and able to adapt to its environment. Several previous studies showed that extracts of H. atra from different waters have activity as an antifungal. Fungus Candida albicans is one of human pathogens that attack on the mucosa of the mouth, skin and vagina. The purpose of this study were to identify the compounds contained in the extracts of H.atra and examine the effect of extracts concentration against C. albicans. The process of extraction was done by maceration with solvent n-hexane, ethyl acetate and methanol. Testing of secondary metabolites was carried out using phytochemical screening methods while testing antifungal activity was using agar diffusion test. The results showed that the extracts of H.atra contained saponins, alkaloids, steroids and triterpenoids. Antifungal activity assays showed that the n-hexane extract did not show any inhibition zone, while the ethyl acetate and methanol extracts showed inhibition zone at a concentration of 1 mg / disk with a large zone of inhibition of 8.27 ± 0.06 and 8.07 ± 0 , 12 mm, respectively based on these results it can be concluded that the extract of ethyl acetate solvent H.atra has strong potential as antifungal.
BAKTERI SIMBION GASTROPODA PLEUROPLOCA TRAPESIUM DARI PERAIRAN TERNATE, SEBAGAI ALTERNATIF ANTIBAKTERI MDR (BACTERIAL SYMBIONT GASTROPODA PLEUROPLOCA TRAPEZIUM FROM TERNATE, AS ALTERNATIVE ANTIBACTERIAL MDR) Pringgenies, Delianis; Renta, Person Pesona
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 1 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.195 KB) | DOI: 10.14710/ik.ijms.19.1.55-62

Abstract

Bakteri yang resisten terhadap beberapa jenis antibakteri ini dikenal dengan bakteri multi drug resistant (MDR).Untuk mengatasi permasalahan tersebut, perlu dilakukan pencarian senyawa antibiotik baru yang lebih efektif dan efisien dalam mengatasi permasalahan bakteri MDR. Penelitian bertujuan untuk mengetahui potensi bakteri yang bersimbiosis dengan gastropoda Pleuroploca trapezium sebagai sumber antibakteri MDR. Sampel Moluska dikoleksi dari perairan Ternate, Maluku. Tahapan penelitian meliputi isolasi bakteri, skrining  bakteri simbion yang potensi sebagai anti bakteri MDR, uji antibakteri, isolasi bakteri patogen klinis MDR; uji sensitivitas anti-bakteri, ekstraksi, amplifikasi dan sekuensing DNA. Hasil 16S urutan r-DNA dianalisis dan diedit menggunakan program Genetix dan diikuti dengan analisis urutan 16S rDNA. Hasil menunjukkan bahwa terdapat 19 isolat bakteri dengan 5 bakteri aktif yang berasosiasi dengan Pleuroploca trapezium. Berdasarkan besarnya zona hambat yang dibentuk dan konsistensi munculnya zona hambatan, isolat terbaik adalah TPT 4.7. Isolat ini memiliki hubungan yang dekat dengan Paracoccus  sp. MBIC4019 dengan homologi sebesar 95% yang menunjukkan kekerabatan ditingkat genus. Hasil penelitian ini memberikan harapan adanya potensi besar sebagai bahan antibakteri baru. Kata kunci: antibakteri, simbion, Pleuroploca trapezium, multi drugs resistantThe bacteria resistant to some antibiotics are known as multi drug resistant (MDR). To overcome the problem, it is needed to search for a new antibiotic compounds more effectively and efficiently. This study aims to identify potential from symbionts of Pleuroploca trapezium as a source of antibacteria MDR and identifying the bacteria that were active against the MDR. Samples were collected from Ternate, Maluku. Isolation of symbiotic bacteria, screening for bacteria which producing secondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria of MDR. Conducting anti-bacterial sensitivity test,  sensitivity test for antibacterial,  DNA exctraction, DNA amplification based on PCR method, DNA sequencing.  Result of 16S r-DNA sequence was then analyzed and edited using GENETYX program and followed by 16S rDNA sequence analysis. Screening of bacteria associated with P. trapezium resulted in 19 isolates with 5 active bacteria. Based on the size of the zone forming and the consistency of zone, so the best isolate is TPT 4.7. The identification shows that TPT 4.7 has a close relationship with the Paracoccus sp. MBIC4019 with homologi of 95%, which shows the relationship at the genus level. Its suggest that these results are very promising as a new antibacterial material. Keywords: antibacterial, symbiotic bacteria, Pleuroploca trapezium, multi drugs resistant