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POLIMORFISME G.-371T>A PROMOTOR GEN MIOSTATIN PADA SAPI PEDAGING INDONESIA Sutikno, Sutikno; Priyanto, Rudy; Sumantri, Cece; Jakaria, Jakaria
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.2.239

Abstract

Myostatin (MSTN) gene acts as a negative regulator of muscle growth. The aim of the present study was to identify polymorphism of g.-371T>A in promoter region of MSTN gene in Indonesian beef cattle. Blood samples were collected from 191 cattle, including Bali (BL) (42), Madura (MD) (20), Pesisir (PI) (17), Katingan (KT) (16), Ongole grade (PO) (22), Pasundan (PD) (14), Sumba ongole (SO) (10), Brahman (BH) (17), Simmental (SM) (15), and Limousin (LM) (18). Polymorphism of MSTN gene was analyzed using PCR-RFLP (DraI) and direct sequencing methods. Results of genotyping g-371T>A were polymorphic (TT, TA, and AA genotypes) in Simmental, SO, and Katingan. The frequencies of alleles T and A were 0.83; 0.90; 0.97 and 0.17; 0.10; 0.03 respectively. The values of Ho and He were 0.06?0.20 and 0.06?0.28, respectively. The results of this study are in Hardy-Weinberg equilibrium (P> 0.05). Whereas in PD, MD, PI, PO, BH, LM, and BL were monomorphic (TT genotype). The result of sequencing the promoter region of MSTN gene found that mutations transversion was occurred in T to A at g.-371. It was concluded that g.-371T>A of MSTN gene was polymorphic which was potential to be used as genetic markers of muscle growth in SM, SO, and KT cattle. Keywords: SNP g.-371T>A of MSTN gene, Sumba ongole cattle, Katingan cattle, Simmental cattle
KUALITAS KARKAS BABI POTONG YANG DILAHIRKAN DARI INDUK YANG DISUPEROVULASI SEBELUM PENGAWINAN (CARCASS QUALITIES OF FINISHER PIG BORN TO SUPEROVULATED SOWS BEFORE MATING) Lapian, Mien Theodora Rossesthellinda; Siagian, Pollung Hasiholan; Manalu, Wasmen; Priyanto, Rudy
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

An experiment was conducted to study the effect of superovulation of the sows before mating on thecarcass quality of the finishing piglets.  Forty two gilts were divided into two treatments i.e., 1)nonsuperovulate gilts and 2) superovulate gilts.  At parturition, each group of sows were divided into threelitter size ranges i.e., 6-8 (low), 9-11 (medium), and 12-14 (high), with three replications.  Each replicationwas represented by two offsprings, one male and one female.  Therefore, the total samples were 36 piglets.During growing to finishing periods, the experimental piglets were fed commercial feed.  The experimentalpiglets were slaughtered at average weight of 92,5- 94 kg.  The results showed that piglets born tosuperovulated sows reached the slaughter weight at 185 days as compared to 200 days in control piglets.Piglets born to superovulated sows had higher carcass weight (70,25 ± 2,70 kg) and carcass percentage(74,73 ± 3,09%) as compared to control piglets (64,18 ± 3,58 kg carcass weight and 68,28 ± 3,90% carcasspercentage, respectively).  Carcass length and  backfat thickness were not affected by superovulation.Piglets born to superovulated sows had 74,56 ± 4,72 cm carcass length and 3,07 ± 0.35 cm backfatthickness, while control piglets had 74,00 ± 2,81 cm carcass length and 3.20 ± 0.35 cm backfat thickness.Piglets born to superovulated sows had higher  loin eye area (44,81 ± 3,55 cm²) as compared to control(39,97 ± 4,29 cm²).  It was concluded that superovulation of sows prior to mating could produce fastergrowing piglets with better carcass qualities
KERAGAMAN SIFAT KUALITATIF PADA SAPI SILANGAN PO DAN BELGIAN BLUE MENGGUNAKAN ANALISIS KOMPONEN UTAMA Jakaria, Jakaria; Zulkipli, Fuadi; Edwar, Edwar; Ulum, Mokhamad Fakhrul; Priyanto, Rudy
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 1 (2020): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v7i1.8675

Abstract

The purpose of this study was to determine the diversity of qualitative traits in PO, Belgian Blue and PO-Belgian Blue crosses using principal component analysis. The total samples of cattle used were 36 consisting of 8 heads Belgian Blue (4 males and 4 females), 17 heads Belgian PO-Belgian cross-breed (11 males and 6 females) and 11 PO heads (all females)). The diversity of qualitative traits observed were 17 characteristics namely plain body-color (WTP), combination body-color (WTK), horned (MT), humped (MP), sagging (MG), black tail tip color (WUEH), white tail tip color (WUEH), black eyelash color (WBMH), white eyelash color (WBMP), circle hair head (PRK), circle hair back (PRP), circle hair bracket (PRB), black nail color (WKH), nail color white (WKP), double muscle (PG), black muzzle (WMH) and white muzzle (WMP). Qualitative data were analyzed descriptively by calculating the proportion value, while the determinant variables of each breed were analyzed using the principal component analysis method (AKU) with the Minitab version 7 program. The results showed that the characteristics of qualitative traits in PO, Belgian Blue, and PO-Belgian Blue cross-breed cattle have a high diversity. PO and Belgian Blue cattle breeds can be clearly distinguished based on the characteristics of qualitative traits, whereas PO-Belgian Blue crossbred cattle have clusters that are close to PO cattle clusters. Found character traits for each cattle breed in both PO, Belgian Blue, and PO-Belgian Blue cross-breed. There are three characteristics of qualitative traits possessed in each cow nation, namely the variable circle hair head (PRK), circle hair back (PRP) and horned characters (MT). Based on the results obtained that the qualitative traits in cattle can be used as a distinguishing character between PO, Belgian Blue, and PO-Blue Belgian cross cattle breeds.
EVALUASI KINERJA PERTUMBUHAN SAPI SILANGAN BELGIAN BLUE DAN PERANAKAN ONGOLE Jakaria, Jakaria; Edwar, Edwar; Ulum, Mokhamad Fakhrul; Priyanto, Rudy
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.15022

Abstract

ABSTRAK. Tujuan penelitian ini adalah mengevaluasi kinerja pertumbuhan sapi persilangan Belgian Blue (BB) dan sapi Peranakan Ongole (PO) pada turunan pertama (F1). Sapi persilangan (BB dan PO) yang digunakan sebanyak 15 ekor terdiri atas jantan 6 ekor dan betina 9 ekor, selain itu digunakan 8 ekor sapi PO betina yang dipelihara di Balai Embrio Ternak (BET) Cipelang Bogor. Peubah yang diamati adalah bobot lahir (BW), bobot sapih (WW) 205 hari, bobot 1 tahun (YW), pertambahan bobot badan harian dari lahir (GBW) sampai sapih (GWW) dan pertambahan bobot badan harian dari sapih (GWW) sampai umur 1 tahun (GYW). Data dianalisis secara deskriptif dan perbedaan antar sapi silangan (BB-PO) dengan sapi PO dilakukan uji t. Hasil analisis bobot badan dan pertumbuhan menunjukkan bahwa, bobot badan sapi silangan BB dan PO lebih tinggi dibandingkan dengan sapi PO pada WW 205 hari (165,2±21,3 kg dan 115±15,2 kg), YW (365 hari) (240,7±28,1 kg dan 194,9±26,1 kg) dan GBW sampai GWW (0,675±0,097 kg dan 0,441±0,059 kg). BW dan GWW sampai GYW tidak berbeda antara sapi silangan BB-PO dengan PO. Hasil penelitian ini menunjukkan bahwa program cross-breeding sapi BB dengan PO dapat meningkatkan kinerja bobot badan dan pertumbuhan.  (Growth performance evaluation of belgian blue and ongole crossbreed) ABSTRACT. The purpose of this study was to evaluate the growth performance of cross-breeding of Belgian Blue (BB) and Ongole Grade (PO) cattle in the first generation (F1). Crossbred of BB and PO cattle used as many as 15 individuals consisting of 6 males and 9 females, also, 8 individual PO females are used which are kept in the Livestock Embryo Center (BET) Cipelang, Bogor. The observed variables were birth weight (BW), weaning weight (WW) 205 days, 1-year weight (YW) 365 days, daily body weight gain from birth (GBW) to weaning (GWW) and daily body weight gain from weaning (GWW) until the age of 1 year (GYW). Data were analyzed descriptively and the difference between cross-breeding (BB-PO) cattle and PO cattle was analyzed by t-test. The results of body weight and growth analysis showed that crossbred BB and PO cattle were higher than PO cattle at WW 205 days (165.2 ± 21.3 kg and 115 ± 15.2 kg), YW (365 days) (240.7 ± 28.1 kg and 194.9 ± 26.1 kg ) and GBW to GWW (0.675 ± 0.097 kg and 0.441 ± 0.059 kg). BW and GWW to GYW do not differ between crossbred BB-PO and PO cattle. The results of this study indicate that the cross-breeding program for BB cattle with PO cattle breeds can improve the performance of body weight and growth.
Energy Balance and Blood Metabolites Status of Local Sheep Based on Indigofera sp and Sproutbean Waste Ration Astuti, Dewi Apri; Rahayu, Sri; Satoto, Kukuh Budi; Priyanto, Rudy; Khotijah, Lilis; Suryati, Tuti; Baihaqi, Muhammad
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol 16, No 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.16.3.43-46

Abstract

This research was undertaken to evaluate energy utilization and blood  metabolites status of male Indonesian local sheep involving nutrient balances and using urea space techniques for measurement body composition. Factorial randomized completely design was used in this study using 16 growing male local sheep. Factor A was breed which consisted of 8 Garut sheep (av.BW 14.90 kg) and 8 Jonggol sheep (av.BW 13.60 kg). Factor B was different ration which received of diet containing Indigoferasp. and sproutbean waste. The ration offered were pellets containing CP around 16% and energy 15.6 MJ GE/kg. Energy balance and blood metabolites studies were conducted during three-months trial. Intake, digestibility, urinary and metabolism variables were measured based on total collection method.  Methane energy was measured by rusitec technique and methane gas production multiplied by calory equivalent value of methane. Energy retention (RE) was measured by urea space  technique. Heat production was calculated from ME-RE. Plasma glucose, cholesterol and urea-N were measured by using spectrophotometry. Results showed that there were signiϐicant differences of energy intakes, digestibilities and metabolism among the rations. Animals fed with sproutbean waste ration has higher energy utilization compared to those fed Indigofera sp ration. There were no signiϐicant different for all blood metabolite variables among breed and its interaction between combination treatments. It is concluded that sproutbean waste in local sheep ration has higher energy utilization and daily gain than Indigofera sp.
KARAKTERISTIK FISIK DAN MIKROSTRUKTUR OTOT SEMITENDINOSUS PADA SAPI LOKAL DAN SAPI IMPOR (PHYSICAL AND MICROSTRUCTURE CHARACTERISTICS OF SEMITENDINOSUS MUSCLE IN LOCAL CATTLE AND IMPORTED COWS) Safitri, Aldina; Priyanto, Rudy; Adnyane, I Ketut Mudite; Nuraini, Henny
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.4.488

Abstract

Semitendinosus muscle is widely used as raw material for traditional culinary in Indonesia. Studies on local beef cattle potency have been widely conducted, however there is limited information on physical and microstructures characteristics of semitendinosus muscle.  This study aimed to examine physical and microstructures characteristics of semitendinosus muscle from different breeds of local and import beef cattle.  This study used semitendinosus muscle from angus cross cattle, bali cattle, brahman cross cattle, PO cattle, and simmental X PO cattle, with age ranging from 18-30 months (I1-I2).  The results showed that semitendinosus muscle of local breed cattle could meet the criteria of physical characteristics of consumer demand in Indonesia. Based on perimysium thickness and fascicle area of the cross section of semitendinosus muscle, angus cross cattle and bali cattle had softer muscle texture compared to the other breeds. 
EVALUASI KESESUAIAN LINGKUNGAN BERDASARKAN PENAMPILAN PRODUKSI EMPAT BANGSA SAPI PADA KETINGGIAN BERBEDA DI PROVINSI LAMPUNG Suretno, Nandari Dyah; Purwanto, Bagus Priyo; Priyanto, Rudy; Supriyatna, Iman
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.3.478

Abstract

Each cattle breed can grow optimally at suitable environmental condition. bali cattle has better thermoregulation ability in incompaing with ongole crossbreed cattle in lowland. Simental or limousin cattle is from temperate zone with cold temperature area and intensive breeding management. Based on those considerations, this research aimed was to investigate the production performance of bali cattle, ongole crossbreed, limousine crossbreed and simental crossbreed cattle at different altitudes and seasons in Lampung Province. Research used adult female cattle consisting of 82 bali cattle, 138 ongole crossbreed cattle, 54 limousin crossbreed cattle and 32 simental crossbreed cattle. The observed variables as production response were body height, chest size and Body Condition Score (BCS). Data was then analyzed using Randomized Complete Design. Based on body size (height and chest circumference) and BCS, it can be concluded that the Bali cattle suitable to be developed in the lowlands, ongole crossbreed cows in the highlands, limousine crossbreed in the lowlands and simmental crossbreed suitable to be developed in both the lowlands and highlands. ABSTRAK Sapi bali mempunyai kemampuan termoregulasi yang lebih baik dibandingkan dengan sapi peranakan ongole di dataran rendah. Sapi simental atau limousin terbiasa hidup di daerah dengan suhu udara yang dingin dan tatalaksana pemeliharaan intensif. Berdasarkan hal tersebut maka tujuan penelitian ini adalah mengetahui kemampuan produksi sapi bali, sapi peranakan ongole, sapi peranakan limousin, dan peranakan simental pada beberapa ketinggian tempat dan musim yang berbeda di Provinsi Lampung. Materi yang digunakan adalah sapi betina dewasa: yaitu sapi bali 82 ekor, sapi peranakan ongole 138 ekor, sapi peranakan limousin 54 ekor, dan sapi peranakan simental 32 ekor. Peubah yang diamati untuk respons produksi adalah tinggi badan, lingkar dada, dan Body Condition Score (BCS). Penelitian ini menggunakan Rancangan Acak Lengkap. Hasil penelitian menunjukkan bahwa berdasarkan ukuran tubuh (tinggi dan lingkar dada) serta BCS, sapi bali cocok dikembangkan di dataran rendah. Sapi PO mempunyai ukuran tubuh sama pada ketiga ketinggian tempat namun BCS terbaiknya di dataran tinggi. Sapi peranakan limousin ternyata penampilan produksi terbaiknya baik musim hujan maupun musim kemarau di dataran rendah. Sementara sapi peranakan simental cocok dikembangkan di dataran rendah dan dataran tinggi.
EFEKTIVITAS MINYAK IKAN LEMURU TERPROTEKSI TERHADAP POPULASI MIKROB RUMEN DAN FERMENTASINYA PADA KERBAU DAN SAPI (THE EFFECT OF PROTECTED LEMURU FISH OIL ON RUMEN MICROBES AND ITS FERMENTATION IN BUFFALOES AND CATTLE) ., Yurleni; Priyanto, Rudy; Gurnadi, Eddy; Wiryawan, Komang Gede
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this experiment was to examine the effect of protected lemuru fish oil on rumenmicrobes and its fermentation  in buffalo and cattle fattened in feedlot.  Six  male buffaloes and eight malecattle aged between 1,5-2 years old with initial live weight of 218,66±16,28 kg and 217,37±15,44 kg,respectively, were used in this study. They were fattened in feedlot using 35% forage and 65% concentratediet for 2,5 months. The protected lemuru fish oil was in the form of dried carboxylate salt mixture (DCM)and given in the form of concentrate mixture,  45 g DCM/kg concentrate. The feeding treatments includedPO (forage+concentrate) dan P1 (forage+concentrate+DCM). At the end of feeding trial, the animals wereslaughtered and the rumen liquor were collected to analyze rumen microbes and its fermentation.  Thedata were  analyzed using a completely randomized design with 2x2 factorial models, feeding trial (PO and P1) as the first factor  and  animals as second factor (buffaloes and cattle). The results showed that nointeraction was found between the two factors on  rumen pH, NH3 concentration, partial VFA, and rumenmicrobes. There were interaction effect of  diets treatment and animal species on total VFA. Total VFAconcentration in buffaloes  with DCM supplementation was significantly higher (P<0,05) than cattle  withor without DCM supplementation. DCM supplementation decreased rumen pH and increased total VFA.The buffaloes had significantly higher total VFA, proteolitic bacteria, and butiric acid,  but  had lowerrumen pH and protozoa population compared to those of cattle. It is concluded that DCM supplementationcan increase the effectivity of   protected fish oil concentration and proportion of VFA as well as rumenmicrobial population.
PENGUKURAN MORFOMETRIK SAPI PERANAKAN ONGOLE DAN KERBAU JANTAN DENGAN METODE CITRA DIGITAL (MORPHOMETRIC MEASUREMENT OF MALE ONGOLE CROSSBRED CATTLE AND BUFFALO BY DIGITAL IMAGE ANALYSIS ) Hilmawan, Fiqy; Nuraini, Henny; Priyanto, Rudy; Putra, Bramada Winiar
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objectives of this study were to compare the methods of animal morphometricmeasurementsusing manual and digital image analysis methods on the identify of ongole crossbred cattle and buffalo. Asmany as38 maleongole crossbred cattle and 44 male buffalos were used in this study. Fifteen bodyparameters were examined and analyzed and the data obtained were analyzed by t-student to determinethe differences between of two measurement methods. The morphometric measurement of ongole crossbredcattle and buffalo by manual was not significantly different (P>0.05) as compared to that of by digitalimaging. Therefore the digital imaging analysis method could be used as an alternative morphometricmeasurement forlarge ruminant animalal such as cattle and buffalos. Based on body morphometric bydigital image analysis showed that ongole crossbred cattle has ossa radius-ulna, os metacarpale, ossatibia fibulla, os metatarsale, hip height and body height longer than buffalo (P<0,05). Depth chest ofbuffalo deeper than ongole crossbred cattle (P<0,05). Body measurement of livestock animal by digitalanalysis method could be applied as it has similar accuracy with the manual method. Ongole crossbredcattle has good potency as beef cattle because it has higher body size proportion than buffalo.
EVALUASI PENERAPAN STANDAR SANITASI DAN HIGIEN DI RUMAH POTONG HEWAN KATEGORI II (EVALUATION OF SANITATION AND HYGIENE STANDARD IMPLEMENTATION AT CATEGORY II ABATTOIR) Gaznur, Zikri Maulina; Nuraini, Henny; Priyanto, Rudy
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.1.107

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Meat is one of livestock industry products from abattoir. The existence of abattoir is necessary to ensure the meat product produced is safe, healthy and halal. This study was conducted to evaluate sanitation and hygiene standard implementation in category II abattoir. Total plate count (TPC), Salmonella sp, Coliform, and Escherichia coli were analized by using Bacteriological Analytical Manual (BAM) method. Analisis of water quality and liquid waste was done according to American Public Health Association (APHA) method. Based on laboratory test on TPC, Salmonella sp, Coliform, and Escherichia coli, the test results did not exceed the limit standard of SNI 3932:2008. The result of water quality and liquid waste analysis was around the threshold set by Indonesian Republic?s Regulation of Health Ministry No. 492/Menkes/Per/IV/2010 on the Quality Requirements of Water and Environment Regulation Ministry No. 5/2014 regarding Standard Liquid Waste. ABSTRAK Daging adalah salah satu produk industri peternakan dari usaha pemotongan hewan. Permintaan masyarakat terhadap daging sapi memengaruhi intensitas pemotongan, sehingga keberadaan rumah pemotongan hewan (RPH) diharapkan dapat menjamin kualitas daging secara aman, sehat dan halal. Penelitian ini bertujuan untuk mengevaluasi penerapan standar sanitasi dan higien di RPH kategori II. Pengujian total plate count (TPC) daging, Colliform, Escherichia coli, dan Salmonella sp berdasarkan metode Bacteriological Analytical Manual (BAM). Pengujian air bersih dan limbah cair menggunakan metode American Public Health Association (APHA). Hasil analisis mikrobiologi menunjukkan bahwa TPC, Salmonella sp, Coliform dan E. coli berada di bawah persyaratan SNI 3932:2008. Hasil analisis air bersih dan limbah cair sudah memenuhi baku mutu Peraturan Menteri Kesehatan Republik Indonesia 492/Menkes/Per/IV/2010 tentang Persyaratan Kualitas Air Bersih dan Peraturan Menteri Lingkungan Hidup 5/2014 Tentang Baku Mutu Air Limbah.