Dwi Priyowidodo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Studi Respon Imun Humoral Mencit, Tikus, dan Ayam terhadap Infeksi Toxoplasma Gondii Ratnaningrum, Gifti Rosalina; Meida, Dana; Putri, Andhini Mutiara; Devi, Hilda Syara Shita; Priyowidodo, Dwi
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6673.961 KB) | DOI: 10.22146/jsv.27556

Abstract

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. T. gondii is an obligate intracellular parasite that is transmitted by congenital and acquisition. T. gondii can infect all warm-blooded animals and are zoonotic. It is estimated that more than a third of the world’s population is infected by T. gondii. Chicken is one of the animals that can be infected with T. gondii and plays an important role in the spread of this disease to humans because it is one of the animals used as a source of animal feed. Mice and rats are also play an important role in the spread of toxoplasmosis in the definitive host, especially in cats. T. gondii infection is usually asymptomatic, but in certain circumstances, such as when the host’s immune system is weakened, this parasite can causeserious illness. This study were aimed to determine the humoral immune response against T. gondii infection in mice, rats and chickens by serological Latex Agglutination Test (LAT) using Pastorex-Toxo® kit. Two weeks oldbroiler chickens were 8 heads, Wistar strain rats aged 8 weeks as many as 18 heads and strain Balb C mice aged 8 weeks as many as 13 cows used in this study. Animals were grouped into two groups, namely the control andinfection groups. Mice, rats, and chickens were infected each with 103, 107 and 105 takizoit by intra-peritoneal injection. Blood samples were taken from the animals and infection control from day to-1, 2, 3, 4, 5, 6 for mice,the to-1, 2, 3, 4, 5, 6, 7, 8 for rats, and day - 2, 4, 14 for chicken by intracardia 3 ml, then put in a non-EDTA tubes and centrifuged at a speed of 10,000 rpm for 5 minutes to collect serum. The results showed a humoral immuneresponse against T. gondii in rats began to appear on day 5 post-infection, chickens began to emerge the 2nd day post infection, whereas mice do not indicate a humoral immune response against T. gondii post infection.
The Effect of Red Fruit's (Pandanus conoideus) Extract to Eritrocytes, Hemoglobin, PCV and TPP Concentrations of Mice (Mus musculus) Infected by Toxoplasma gondii Utami, Anis Dwi; Priyowidodo, Dwi
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.413 KB) | DOI: 10.22146/jsv.5418

Abstract

Toxoplasmosis is a zoonotic parasites caused by Toxoplasma gondii. The red fruit’s extract contains tocopherol and betacarotene as antioxidant and it can repair the bodies immune system. This research was aimed to study the effect of red fruit’s extract to erithrocytes, hemoglobine, the values of PCV and TPP concentration of mice infected with T. gondii, so that the red fruit extract can be used as alternative herbal medicine for eliminating Toxoplasmosis. 15 of Balb/C mice, male, three months, 30 gr of body weight were used as experimental animals. The mice were then devided into three groups. Group I was a control, group II  was tachyzoite infected and group III was tachyzoite infected and given red fruit’s extract. Group III given red fruit’s extract on first day with 0,53 g/kg BW/mice/day. Mice in group II and III were infected by injection Toxoplasma gondii tachyziote 1x103 a mice intraperitoneally. The blood sample was taken from five mice in each group at 7th and 12th day pass through cantus medialis. The blood was examinated in Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Data were analyzed by using Factorial 2 x 3. The result showed no significant difference (P>0,05) in hemoglobin, and PCV’s value between groups I, II and III before and after infection of T. gondii’s tachyzoite. The TPP’s value between groups I, II and III before and after infection of T. gondii’s tachyzoite was significantly difference (P<0,05).
PARASIT GASTROINTESTINAL PADA SAPI DI DAERAH ALIRAN SUNGAI PROGO YOGYAKARTA (THE GASTROINTESTINAL PARASITES COWS ON PROGO WATERSHED IN YOGYAKARTA) Nugraheni, Yudhi Ratna; Priyowidodo, Dwi; Prastowo, Joko; Rohayati, Eryl Sri; Sahara, Ana; Awaludin, Aan
Jurnal Ilmu Peternakan Terapan Vol 1, No 2 (2018)
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipter.v1i2.889

Abstract

INTISARIPenelitian ini bertujuan untuk mengidentifikasi parasit gastrointestinal pada sapi yang berlokasi di daerah aliran Sungai Progo. Penelitian ini menggunakan feses sapi sebanyak 63 sampel yang diambil dari daerah aliran Sungai Progo. Pemeriksaan feses menggunakan metode sentrifuse, dan Parfitt Bank. Hasil penelitian menunjukkan bahwa  63 sampel feses sapi yang diperiksa, 25 diantaranya terinfeksi cacing jenis Fasciola sp., 7 ekor Paramphistomum sp., 14 Strongyle, dan 9 Coccidiosis.  Kesimpulan dari penelitian ini adalah prevalensi parasit gastrointestinal pada sapi di aliran Sungai Progo yang dominan adalah kejadian Fasciolosis sebesar 40%, infestasi nematodiasis jenis Strongyle 22%, Coccidiosis 14%, dan infestasi Paramphistomum sp.11%. Kata kunci: Coccidiosis, Fasciola sp., Parampistomum sp., Strongyle   ABSTRACTThe aim of study was investigate gastrointestinal disease on cow fed forage from Progo watershed. Sixty three feses samples were taken from cows on Progo watershed. The method of feses analysis used was sentrifuge and Parfitt Bank. The results showed that 63 samples of cows feces were examined, among 25 were infected by Fasciola sp., 7 Paramphistomum sp., 14 Strongyle, and 9 Coccidiosis. The conclusion was the prevalence of gastrointestinal disease in cows was dominant incidence of Fasciolosis by 40%, Strongyle 22%, 14% Coccidiosis, and Paramphistomum sp.11%. Keywords: Coccidina, Fasciola sp., Parampistomum sp., Strongyle
Identifikasi Cacing Trematoda dan Gambaran Patologi Ginjal Burung Merpati yang Terinfeksi (IDENTIFICATION AND PATHOLOGICAL FEATURES OF TREMATODE IN PIGEON’S KIDNEY) Sahara, Ana; Prastowo, Joko; PriyoWidodo, Dwi; Rohayati, Eryl Sri; Widyarini, Sitarina
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.803 KB)

Abstract

This study is conducted to identify species of trematode and its pathological features in pigeons’kidney. Twenty five of Yogyakarta pigeons were examined for trematode infection in kidneys. One ofkidneys was mashed in mortar with a little water, the other was examined for histopathological featuresstained with hematoxyline-eosin.Trematodes found were stained with Schmison ´s Carmine. Seven pigeons(28%) were infected by trematode with non significant clinical features. Identification trematodescharacterized by oral sucker, pharynx, testes are slighty diagonal in position, irregular in shape and intracaecal. Ovary pretesticular and vitellaria widespread from anterior ovarium to the posterior body.Histopathological examination showed segment of trematode in the medullary collecting ducts lumen,dilatation, flattening and emptyness of ducts epithelial cells, emptyness, giant cells and dominationmononuclear cell in interstitial tissue, characterizing a granulomatous nephritis. Trematodes foundidentified as Paratanaisia bragai. The trematodes were found in very dilated medullary collecting ductsand caused inflammation in adjacent tissues. Further studies are needed to find out vector of trematodein pigeons .
Efektivitas Pemberian Vaksin Koksidia Melalui Air Minum Dan Pakan Priyowidodo, Dwi
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2134.824 KB) | DOI: 10.22146/jsv.358

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas penggunaan vaksin koksidia yang diberikan melalui air minum dan semprot pakan terhadap infeksi tantangan E. tenella. Sebanyak 60 ekor anak ayam pedaging umur satu hari dipakai sebagai hewan percobaan. Ayam dibagi menjadi 3 kelompok secara acak, tiap kelompok terdiri dari 20 ekor. Pada umur 5 hari, kelompok II divaksin dengan Coccivac-D® yang diberikan melalui semprot pakan, kelompok III divaksin dengan Immucox® diberikan lewat air minumda kelompok I (kontrol) tidak divaksin. Duapuluh satu hari sesudah perlakuan, ayam dari kelompok I,II,dan III ditantang dengan 3.000 oosista E. tenella. Lima hari sesudah infeksi, 10 ekor ayam dari masing-masing kelompok dinekropsi untuk melihat derajat perlukaan sekum. Hari ke-7 sampai ke-11 setelah infeksi, tinja ayam diperiksa dengan metode McMaster untuk menghitung jumlah oosista per gram tinja. Data derajat perlukaan sekum dianalisis dengan metode Rank test, sedangkan data jumlah eliminasi oosista dianalisis dengan metode Split plot (Gill, 1978). Kesimpulan penelitian menunjukkan pemberian vaksin Coccivac-D® melalui semprot pakan lebih baik juka dibandingkan dengan pemberian Immucox® melalui air minum..
DISTRIBUSI Culicoides spp. (DIPTERA: CERATOPOGONIDAE) PADA PETERNAKAN AYAM PETELUR DI KABUPATEN SLEMAN YOGYAKARTA = A DISTRIBUTION OF Culicoides spp. (Dag bRA: CERATOPOGONIDAE) ON THE LAYER POULTRY FARMS IN SLEMAN REGEN Sahara, Ana; Priyowidodo, Dwi
Jurnal Sain Veteriner Vol 20, No 1 (2002): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5330.004 KB) | DOI: 10.22146/jsv.315

Abstract

Telah diteliti distribusi spesies Culicoides yang ada di kitar peternakan ayam petelur di Kabupaten Sleman Daerah Istimewa Yogyakarta. Penelitian ini ditujukan untuk mengetahui spesies-spesies Culicoides yang mempunyai peranan dalam menyebarkan penyakit leukositozoonosis pada ayam petelur. Sebanyak 762 ekor Culicoides (Diptera: Ceratopgonidae) dikumpulkan dari dua belas peternakan ayam petelur di wilayah Kabupaten Sleman dengan menggunakan perangkap serangga Pirbright type rniniatur light trap. Culicoides yang diperoleh dikelompokkan dan diidentiflkasi berdasarkan karakter morfologis menurut Wirth den Hubert. Basil penelitian menunjukkan tidal( ada perbedaan populasi Culicoides dari petemakan ayam petelur yang ada di wilayah Kabupaten Sleman pada dataran rendah maupun dataran tinggi. Ada delapan spesies Culicoides yang diperoleh dari petenakan ayam petelur di Kabupaten Sleman yang berhasil diidentifikasi yaitu: C. lye, C. guttifer, C. arakawae, C. oxystoma, C. parahumeralis, C. peregrinus, C. sumatrae dan C. clavipalpis. Spesies Culicoides yang dicurigai sebagai penyebar penyakit leukositozoonosis di Kabupaten Sleman adalah C. huffi, C. guitifer , dan C.arakawae .
Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Priyowidodo, Dwi; Hartati, Sri; Kusumawati, Asmarani; Prastowo, Joko
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.315 KB)

Abstract

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.
EHRLICHIOSIS PADA KUCING YANG MENGALAMI ANEMIA DAN INDIKASI GAGAL GINJAL Kurnia, Kurnia; Anggoro, Dito; Budhi, Setyo; Priyowidodo, Dwi
ARSHI Veterinary Letters Vol. 4 No. 2 (2020): ARSHI Veterinary Letters - Mei 2020
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.271 KB) | DOI: 10.29244/avl.4.2.23-24

Abstract

Kucing persia diperiksa sebanyak 2 ekor diperiksa tanggal 23 April 2019 dan 29 April 2019 dengan gejala lesu, mukosa pucat, tidak mau makan sejak 3-5 hari, dehidrasi dan mengalami penurunan berat badan dalam satu bulan terakhir. Kucing tersebut berasal dari pemilik berbeda yang memungkin pernah kontak dengan anjing. Pemeriksaan klinis menunjukkan kedua kucing mengalami anemia, lethargi dan abnormalitas ukuran ginjal yang membengkak pada kucing I dan atropi pada kucing II. Hasil pemeriksaan laboratorium kedua kucing mengalami anemia, SGPT/ALT turun, albumin normal, blood urea nitrogen (BUN) dan creatinin keduanya meningkat. Kucing I mengalami trombositopenia, leukositosis dengan neutrofilia dan protein plasma normal. Kucing II menunjukkan trombosit normal, neutrofilia dan total protein yang meningkat. Pemeriksaan preparat apus darah ditemukan inklusi intrasitoplasmik dalam neutrofil dari kedua kucing yang mengarah pada morula Ehrlichia sp. Diagnosa kedua kucing mengarah pada dugaan Ehrlichiosis. Penanganan anemia dan dehidrasi diberikan infus NaCl 0,9% intravena, injeksi Meylon dan Hematodin. Kondisi kedua kucing terus menurun, kucing I mati setelah 2 hari terapi dan kucing II mati setelah 5 hari terapi.