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COCONUT WATER BASED EXTENDER EFFECTS ON MOTILITY,VIABILITY, AND DNA INTEGRITY OF CHILLED KINTAMANI DOG SEMEN Gunawan, I Wayan Nico Fajar; Kardena, I Made; Suatha, I Ketut; Puja, I Ketut
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 50C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p<0.01), whereas it had no effect on the percentage of dead sperm (p> 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.
PREVALENCE OF EAR AND EYE DISORDERS IN KINTAMANI DOG Perayadhista, Ni Made Devityasih; Suatha, I Ketut; Puja, I Ketut
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

This observational study was undertaken to evaluate the prevalence of ear and eye disorders in Kintamanidog. All data were gathered from registers of veterinary clinic located at Denpasar, Badung, and Gianyar.Various aspects concerning ear and eye disorders were collected by questionnaires. The total of Kintamanidog recorded from 2009 until 2013was 477 dogs. The result of this study showed that the prevalence ofexophthalmos (protrusion of the eye ball) and glaucoma were rare (0%), the cataract was occurred in onedog (0.2%), otitis was occurred in two dogs (0.4%), and conjunctivitis was occurred in three dogs (0.6%).All Kintamani dogs with ear and eye disorders were the male and thier ages were more than 12 months. Inconclusion, this study indicates  that the Kintamani dog has a lower problem of ear and eye disorders. In thefuture, this low prevalence of the problem hopefully can be maintained or even decreased by giving goodbreeding management.
VPH-1 TURNING RESEARCH INTO RESULTS: CHARACTERIZING THE DOG MEAT TRADE IN BALI TO HELP END THE TRADE Sasadara, Maria Maliga Vernandes; Timur, Ni Putu VT; Puja, I Ketut; Dharmawan, Nyoman S; Hood, Jennifer
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The dog meat trade (DMT) in Bali is a black-market trade that threatens international tourism on both animal welfare and human health grounds. Outlets selling dog meat often advertise this by displaying ?RW? signs as this abbreviation stands for ?rintek wuuk? (soft fur) in Manadonese. The DMT is notoriously cruel and uses inhumane methods to catch, transport, and slaughter dogs, many of which are believed to be stolen.Dog meat is not a food under Indonesian law and as such is not subject to food safety or zoonotic disease regulation. In particular, the DMT is believed to contribute to the transmission of rabies, which is endemic in Bali.Despite this, there was little factual information about the DMT in Bali, and this was hindering Government efforts to close the trade. We believe our study is the first to characterize the DMT in Bali and provides insights as to how and why the trade exists, and what actions are needed to end it.
PERCENTAGE OF VIABLE SPERMATOZOA COLLECTED FROM THE EPIDIDYMES OF DEATH LOCAL DOG Sulabda, I Nyoman; Puja, I Ketut
Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The purpose of this study to determine the effectof post mortem time on percentage of lifeepididymessperm from postmortem dog caudae epididymides. A total of 9 dog were usedand divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6hours postmortem. This way, samples were obtained at different times postmortem. Spermwere extracted from the caudae epididymes by means of cuts.The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33 ± 5.60(T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life wasaffected by postmortem time. There was significant decrease life sperm recovered fromepididymes postmortem (P<0.01). In conclusion, epididymes sperm from dog undergodecrease of percentage of life, but it could stay acceptable within many hours postmortem.We intepreted these data to indicate that it may still be possible to obtain viablespermatozoa many hours later.
PENGARUH SUBSTITUSI AIR KELAPA MUDA DENGAN PENGENCER SITRAT KUNING TELUR TERHADAP MOTILITAS DAN PERSENTASE HIDUP SPERMATOZOA ANJING Sulabda, I Nyoman; Puja, I Ketut
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The purpose of this study was to determine the effect of tender coconut water substitutionon egg yolk citrate diluent with different doses on local breeds dog sperm motility and livespermatozoa. Semen was manually collected. Progressive motility and percentage of livespermatozoa were evaluated under a microscope utilizing a drop of semen between awarmed glass slide and coverslip, both at a temperature of 38 0C. The percentage of motileand live spermatozoa were examined by counting 100 spermatozoa using the classificationof Christiansen (1984). Sperm viability was assessed by eosinnegrosin staining. The result showed that coconut water substitution has significant effect on the motility and livespermatozoa . Combination between the levels of coconut water in the egg yolk citratediluent could be applied as an alternative diligent instead of egg yolk diligent for dogsemen up to 75%.
PERILAKU BERMASALAH PADA ANJINGKINTAMANI Fajar, I Wayan Nico; Sukada, I Made; Puja, I Ketut
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Anjing Kintamani adalah sebutan sekelompok anjing yang habitat aslinya di daerahKintamani. Penampilan dan karaketristik yang menarik menyebabkan anjing Kintamanisangat populer sebagai hewan kesayangan dan sekarang sedang diajukan ke FederationCynologique Internationale untuk penetapan sebagai anjing ras. Tujuan penelitian iniadalah mengevaluasi perilaku bermasalah pada anjing Kintamani. Penelitian ini dilakukandari bulan April sampai Mei 2011. Beberapa aspek yang berkaitan dengan masalah perilakudikumpulkan dengan menggunakan quesioner. Sebanyak 46 ekor anjing dari 75 anjing yangdigunakan sebagai sampel menunjukkan perilaku bermasalah (61.3%) dan 29 anjing tidakmenunjukkan perilaku bermasalah (38.7%). Di antara anjing yang digunakan sebagaisampel rata-rata umur anjing adalah 1- 2tahun dan hampir semua anjing belum disterilkan(92%). Juga didapat bahwa pemilik anjing memelihara anjingnya di halaman rumah (36%).Anjing berturut turt menunjukkan suara berlebihan (36%), perilaku merusak (17%), responberlebihan (6,7%), perilaku tidak pantas (34%) dan perilaku agresive (10,7%). Hasilpenelitian ini mendukung pendapat bahwa anjing kintamani tidak mempunyai perilakubermasalah, sehingga diharapkan sifat anjing Kintamani ini tetap dapat dipertahankan danuntuk dijadikan standar perilaku pada anjing kintamani
PERILAKU MERAWAT ANAK PADA ANJING KINTAMANI BALI PRIMIPARA DAN MULTIPARA Manuela, Ni Luh; Puja, I Ketut; Sulabda, I Nyoman
Indonesia Medicus Veterinus Vol 8 (6) 2019
Publisher : Faculty of Veterinary Medicine, Udayana University

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Abstract

Anak anjing yang baru dilahirkan, dalam melanjutkan proses kehidupannya sangat ketergantungan pada induk. Hubungan antara pengalaman induk dan penampilan dalam berinteraksi dengan anaknya akan meningkatkan ketahanan hidup anak secara signifikan. Penelitian ini bertujuan untuk menganalisis perilaku merawat anak pada anjing kintamani bali serta menginvestigasi apakah ada perbedaan perilaku merawat anak antara induk primipara dengan multipara. Sampel penelitian terdiri dari lima indukan primipara dan tujuh indukan multipara dan diambil dari kennel anjing kintamani yang berada di Bali, Solo, Bandung dan Surabaya dengan lingkungan terkontrol. Interaksi induk dan anak dicatat selama 15 menit setiap hari pada hari ke-7, -14 dan -21 setelah melahirkan. Rata-rata waktu berinteraksi antara induk primipara dan multipara dianalisis dengan Student T-test. Hasil penelitian menunjukkan adanya perbedaan yang nyata pada waktu yang diperlukan untuk menyusu antara induk primipara dengan multipara.  Pada indukan multipara, waktu yang dihabiskan untuk menyusu adalah 13,95 menit sedangkan pada induk primipara adalah 9,93 menit. Waktu yang dihabiskan induk untuk kontak dengan anak, seperti menjilat bagian tubuh dan alat genitalis anak tidak menunjukan perbedaan yang nyata. Hasil penelitian disimpulkan bahwa induk anjing kintamani multipara menghabiskan waktu lebih lama dibanding dengan induk primipara.
ASOSIASI LOKUS MIKROSATELIT BMS1282 DENGAN KEJADIAN KAWIN BERULANG PADA SAPI BALI Dewi, Yesy Febnica; Wandia, I Nengah; Puja, I Ketut
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle has an important role to fulfill the meat demands in Indonesia. The improvement of molecular technology will facilitated bali cattle genomic selection. The aim of this research was to verify association between BMS1282 microsatellite locus and repeat breeding in bali cattle. Totalling 52 bali cattle blood samples was taken from UD Sari Laba, Bangli, Bali as DNA resources. DNA was extracted using blood mini Kit qiagen and microsatellite locus was amplified by Polymerase Chain Reaction (PCR). Alleles were separated with 6% PAGE and visualized using silver stain. There were 5 alleles in BMS1282 locus which the alleles size varied from 148 to 156 bp. Allele 152 was the higest frequency (54.81%) and allele 156 was the lowest frequency (1.92%). The value of heterozygosity (Ho), He, and PIC was 0.346, 0.626, and 0.569. consecutively. Statistical analysis revealed that BMS1282 locus has no assosiation with the repeat breeding (P>0.05). This result indicates that BMS 1282 locus has no important role in regulating the repeat breeding.
PELUANG PENGGUNAAN SPERMATOZOA EPIDIDIMIS YANG DIKOLEKSI SETELAH KEMATIAN SEBAGAI SUMBER SEL GAMET PADA ANJING Puja, I Ketut; Trilaksana, IGN; Lontoh, Rudy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.434 KB) | DOI: 10.24002/biota.v10i2.2846

Abstract

The purpose of this study was to determine the effect of postmortem time on percentage of life epididymal sperm from postmortem canine caudae epididymides. A total of 9 dog were used and divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6 hours postmortem. This way, samples were obtained at different times postmortem. Sperm were extracted from the caudae epididymis by means of cuts. The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33± 5.60 (T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life was affected by postmortem time. There was significant decrease life sperm recovered from epididymides postmortem (P<0.01). In conclusion, epididymal sperm from dog undergo decrease of percentage of life, but it could stay acceptable within many hours postmortem. We intepreted these data to indicate that it may still be possible to obtain viable spermatozoa many hours later.
BIOAKTIVITAS GEL ALOE VERA PADA GONAD TIKUS PUTIH JANTAN (RATTUS NORVEGICUS) Suardita, I Ketut; Puja, I Ketut; Pemayun, Tjok Gde Oka
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus). This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD) Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it?has a high possibilities as an alternative herbal contraceptive agent for animals