Trioso Purnawarman
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Institut Pertanian Bogor

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RESISTENSI ANTIBIOTIK TERHADAP BAKTERI ESCHERICHIA COLI YANG DIISOLASI DARI PETERNAKAN AYAM PEDAGING DI KABUPATEN SUBANG, JAWA BARAT (ANTIBIOTIC RESISTANCE TO ESCHERICHIA. COLI ISOLATED FROM BROILER FARMS IN SUBANG DISTRICT, WEST JAVA PROVINCE) Niasono, Aji Barbora; Latif, Hadri; Purnawarman, Trioso
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.2.187

Abstract

Antibiotic resistance in avian bacterial pathogens is a common problem in the Indonesia poultry industry. The present study aimed to provide information on the present status of antibiotic resistance in Escherichia coli (E. coli) from a broiler farm environment. The research method uses a cross sectional study with a stratified random sampling pattern. E. coli was collected from litter using boot swab method at the broiler house farms in Subang District. E. coli isolates (n = 74) were obtained from 74 samples of boot swabs. The sample was isolated and identified morphologically and biochemically using the IMVIC test to obtain E. coli isolates. The test was followed by a sensitivity test for nine antibiotics, namely tetracycline, sulfamethoxazole, trimethoprim, ampicillin, nalidixic acid, ciprofloxacin, enrofloxacin, gentamicin and chloramphenicol by using the agar dilution method. The interpretation of the results of this sensitivity test refers to the Clinical and Laboratory Standards Institute CLSI VET01S (2015) and CLSI M100 (2018). Measurement of the level of knowledge is done using a structured questionnaire that has been tested for validity and reliability. E. coli was isolated from 74 samples of boot swab. 93.2% of E. coli samples were found to be MDR with the highest prevalence in four types of antibiotics at 40.5%. The most common resistances observed were against tetracycline 97.3%, sulfamethoxazole 87.8%, trimethoprim 74.3%, ampicillin 68.9%, nalidixic acid 64.8%, ciprofloxacin 45.9%, enrofloxacin 40.5%, gentamicin 28.4% and chloramphenicol 10.8%. The findings of this study revealed the high level of antibiotic resistance in broiler environment is worrisome and have negative implications for human and animal health.
TRICHINELLOSIS PADA BABI DI KOTA MANADO PROVINSI SULAWESI UTARA Pramono, Syahdu; Satrija, Fadjar; Purnawarman, Trioso
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.403 KB) | DOI: 10.29244/avi.4.1.27-34

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Trichinellosis adalah penyakit zoonnotik yang berasal dari makanan yang disebabkan oleh cacing nematoda Trichinella spp. Penyakit ini masih kurang mendapatkan perhatian di negara maju dan negara berkembang. Parasit ini mempunyai distribusi yang sangat luas hampir di seluruh dunia. Tujuan dari penelitian ini adalah untuk mengkaji keberadaan trichinellosis pada daging babi di Manado. Penelitian ini menggunakan lintas sektional dengan total sampel otot maseter babi (n=139) dan otot diafragma babi (n=139) berasal dari 4 rumah potong hewan babi di Manado. Pengujian laboratorium terhadap Trichinella spp. menggunakan uji pool digesti terdeteksi larva yang diduga Trichinella spp. dalam satu pool yang terdiri dari 9 sampel otot diafragma babi. Pool yang terdeteksi positif kemudian secara individu diuji dengan menggunakan uji kompresi dan dilakukan pembuatan preparat dengan metode pengecatan Hemaktosili Eosin. Hasil dari pengujian individual tidak terdeteksinya Trichinella spp. pada sampel yang diuji. Berdasarkan hasil pengujian tersebut diatas daging babi yang diperiksa tidak terinfeksi oleh Trichinella spp.Kata kunci: digesti, kompresi, Trichinella spp. (Trichinellosis in Pig in Manado North Sulawesi Province)Trichinellosis is a food-borne zoonotic disease caused by the nematode Trichinella spp. However it is still a neglected disease in development and developing country. This parasite has worldwide distribution in a worldwide. The aims of this study were to observe the occurance Trichinella spp. in pork in Manado. The research was conducted using cross sectional study. A total of pig masseter muscle (n=139) and pig diaprhagmatic muscle (n=139) came from 4 slaughter house in Manado. Laboratory examination of Trichinella larvae using pooled sample digestion method was detected one larva in a pooled batch of 9 pig diaprhagmatic muscle samples whereas the suspected positive findings were individually subjected to the compression method and Hematoxilin Eosin staining method. The result showed that the infected  could not be identified. Based on these findings, the absence of Trichinella infection in pigs slaughtered pigs indicates that not infected by Trichinella spp.Keywords: compression, digestion, Trichinella spp.
DETEKSI SALMONELLA SPP. PADA TELUR AYAM KONSUMSI YANG DILALULINTASKAN MELALUI PELABUHAN TENAU KUPANG Nugroho, Susanto; Purnawarman, Trioso; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 3 No. 1 (2015): Januari 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.83 KB) | DOI: 10.29244/avi.3.1.16-22

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Salmonelosis adalah salah satu penyakit food-borne bakterial zoonotik yang paling penting di seluruh dunia. Salmonella spp. adalah penyebab salmonelosis akibat konsumsi makanan berbahan dasar unggas dan produk unggas yang terkontaminasi. Unggas dan telur ayam dianggap merupakan salah satu reservoir Salmonella spp. yang paling penting. Salmonella spp. ditularkan melalui rantai makanan dan akhirnya menular ke manusia. Meningkatkan keamanan produk unggas dengan cara deteksi dini terhadap food-borne patogen merupakan komponen penting untuk membatasi kontaminasi Salmonella spp.. Metode deteksi dan identifikasi Salmonella spp. merupakan strategi yang dirancang untuk mencegah kontaminasi unggas dan produk unggas. Tujuan dari penelitian ini adalah untuk mendeteksi Salmonella spp. dari telur ayam yang berasal dari 4 pengirim telur menggunakan metode konvensional. Jumlah sampel dihitung dengan menggunakan rumus menduga prevalensi dan diambil menggunakan metode acak berlapis. Analisis data hasil positif Salmonella spp. dilakukan secara deskriptif. Berdasarkan hasil pengujian dari 270 sampel, 5 pengujian dengan metode konvensional positif Salmonella spp.. Berdasarkan hasil pengujian positif kontaminasi Salmonella spp. pada telur ayam maka diperlukan evaluasi terhadap pengiriman telur ayam konsumsi antar pulau.Kata kunci: kontaminasi, Salmonella spp., metode konvensional, telur ayam. (Detection of Salmonella spp. in Commercial Hen Eggs Entering through Tenau Port Kupang)Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide. Salmonella spp. are causative agent of salmonellosis associated with contaminated commercial poultry and poultry product. Poultry and eggs are considered one of the most important Salmonella spp. reservoirs. Salmonella spp. were able to pass through the food chain and ultimately transmitted to humans. Improving safety of poultry products by early detection of food-borne pathogens would be considered an important component for limiting exposure to Salmonella contamination. Detection and identification method for Salmonella spp. are considered to be an important component of strategies designed to prevent poultry and poultry product. The aims of the study were to detect Salmonella spp. from hen eggs collected from 4 exporters using conventional method. Samples size were calculated using estimates prevalence formula and selected by stratified random sampling. Data regarding the proportion of Salmonella spp. positive samples were analyzed descriptively. 270 samples, 5 test by conventional method were positive Salmonella spp.. According of positive test results Salmonella spp. contamination in hen eggs was necessary to evaluate the delivery of commercial hen eggs between islands.Keywords: conventional methods, hen eggs, Salmonella spp. contamination.
KEBERADAAN BAKTERI LISTERIA MONOCYTOGENES PADA KEJU GOUDA PRODUKSI LOKAL DAN IMPOR (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES) Pazra, Debby Fadhilah; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey?s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.
VERIFIKASI UJI CEPAT KOMERSIAL ESCHERICHIA COLI PADA CONTOH UJI DAGING SAPI BEKU (VERIFICATION OF ESCHERICHIA COLI COMMERCIAL RAPID TEST KIT ON FROZEN MEAT) Ayunina, Yasmine Qurrota; Purnawarman, Trioso; Setiyaningsih, Surachmi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5088

Abstract

This study was aimed to assess the performance and suitability of commercial rapid test compared to the conventional test through verification process. This study used frozen meat from laboratory routine samples which divided into five groups, those were: natural group, low bacteria level group, medium bacteria level group, high bacteria level group, and control group, each sample test performed 9 replicates. All samples were tested for E. coli by conventional method (SNI 2897:2008) and commercial rapid test method. E. coli test result from both methods was calculated as precision (relative standard deviation), sensitivity, specificity, false negative, false positive, and kappa. The results showed that the commercial kit test had good precision with relative standard deviations score was 0.103. The sensitivity, specificity, false negative, false positive, and kappa score were 94.44%, 100%, 5.56%, 0%, and 0.872, respectively, indicates an equal performance with conventional method. The t student test showed that commercial rapid test method and conventional method had suitability on natural group, low bacteria levels group and medium bacteria level group.
IDENTIFIKASI LISTERIA MONOCYTOGENES PADA SUSU KAMBING DI KABUPATEN PURWOREJO JAWA TENGAH Andriani, Monika Danaparamitha; Purnawarman, Trioso; Damayanti, Retno; Daulay, Syafril
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (989.878 KB) | DOI: 10.22146/jsv.22809

Abstract

Listeria monocytogenes is a pathogenic Listeria species, especially for high-risk groups and it that can be transmitted through contaminated food. Goat milk produced by traditional milking process and storage has a high risk of contamination. The aim of this study was to identify the presence of L. monocytogenes in fresh goat milk in Puworejo regency, Central Java. This study used 60 samples of raw goat milk that were obtained from seven farms by disease detection sampling method. All of the used method in this research refer to Indonesian National Standard (SNI) ISO 11290-1: 2012 about Microbiology of food and feed for detection and enumeration of Listeria monocytogenes. A total of 60 samples of raw goat milk that used in this study were not contaminatedwith L. monocytogenes. Based on the results of this study, it can be concluded that all the samples of raw goat milk were free from L. monocytogenes and have fulfilled the Indonesian National Standard (SNI) ISO No. 7388: 2009 about Limit of Microbial Contamination in Food.
KEBERADAAN KOLIFORM PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DRAMAGA BOGOR Zelpina, Engki; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Teknologi Pangan dan Gizi Vol 19, No 1 (2020)
Publisher : Widya Mandala Catholic University Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jtpg.v19i1.2447

Abstract

Penelitian ini bertujuan untuk mengetahui cemaran bakteri koliform pada daging ayam suwir bubur ayam yang dijual Dramaga Bogor. Sampel daging ayam suwir bubur ayam yang digunakan berasal dari pedagang bubur ayam. Sejumlah 15 sampel daging ayam suwir bubur ayam di isolasi terhadap bakteri koliform. Metode yang digunakan adalah cara tuang (pour plate method). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa jumlah cemaran koliform pada daging ayam suwir bubur ayam yang dijual di Dramaga Bogor adalah 4,25 x 105 ± 2,74 x 105. Cfu/g. Jumlah koliform pada daging ayam suwir bubur ayam melebihi SNI sehingga dapat menimbulkan berbagai gangguan kesehatan masyarakat.
KARAKTERISTIK DAN PENGETAHUAN HIGIENE SANITASI PEDAGANG DAGING AYAM DI PASAR TRADISIONAL DI KOTA JAKARTA Sidabalok, Hasudungan A; Widaya Lukman, Denny; Purnawarman, Trioso
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2794

Abstract

Tujuan penelitian ini mengetahui karakteristik pedagang daging ayam (umur, tingkat pendidikan, pengalaman berjualan, pelatihan, dan statuskepegawaian) di pasar tradisional di Kota Jakarta dan hubungan antara karakteristik tersebut dengan pengetahuan terkait higiene dan sanitasiketika berjualan daging ayam. Sebanyak 217 pedagang daging ayam di pasar tradisional di DKI Jakarta digunakan sebagai responden. Metodepenentuan sampel dengan cluster random sampling dan penentuan sampel pasar dengan probability proportional to size (PPS). Data dianalisissecara deskriptif dan hubungan antara peubah diuji dengan uji Gamma. Hasil yang diperoleh adalah hampir seluruh responden (n= 216; 99,5%)berumur di atas 21 tahun, sebagian besar responden (n= 105: 52,3%) lulus SMP dan SMA, dan kebanyakan responden memiliki pengalamanberjualan 3 tahun. Tingkat pengetahuan responden termasuk dalam kategori sedang sampai dengan baik (n= 209; 96,3%). Hasil analisis datamenunjukkan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P 0,05) dengan kekuatan korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P 0,05) dengan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P 0,05) mempunyai korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P 0,05) mempunyai korelasi lemah (r= 0,296).
RESISTENSI ANTIBIOTIK PADA ESCHERICHIA COLI YANG DIISOLASI DARI DAGING AYAM PADA PASAR TRADISIONAL DI KOTA BOGOR (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR) Januari, Connie; Sudarwanto, Mirnawati Bachrum; Purnawarman, Trioso
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.1.125

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Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health. 
PREVALENCE OF SALMONELLA SPP. BACTERIA ANTIBIOTIC RESISTANCE IN DIGESTION TRACT IN THE BROILER FARMS OF SUBANG DISTRICT Walyani, Septa; Purnawarman, Trioso; Sudarnika, Etih
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.046 KB) | DOI: 10.21059/buletinpeternak.v43i1.41010

Abstract

This study is aimed to  estimate the prevalence of resistant Salmonella spp., determine the spread of bacterial resistance and investigate the serotypes of bacteria in the chicken?s digestion tract in the broiler farms in Subang District. As many as 74 farms were chosen, five poled caeca samples were taken from each farm and tested for isolation and identification of Salmonella spp. Salmonella isolates obtained were tested antimicrobial susceptibility against 8 antibiotics using the agar dilution method. The antibiotics were gentamycin, tetracycline, ciprofloxacin, nalidixic acid, ampicillin, chloramphenicol, trimethoprim, and sulfamethoxazole. The result showed that 8 out of 74 samples were positive for Salmonella. The prevalence of Salmonella spp. in the digestion tract was 10.8%; 95% confidence interval 3.7%-17.9%. Based on the serological test eight serotypes obtained were Salmonella enteritidis, Salmonella oslo, Salmonella narashino, Salmonella nakuru, and Salmonella nordufer. The result of antibiotic resistance test showed that from 8 Salmonella isolates obtained, 12.5% were found to be sensitive, 75% isolates were resistant to one or two antibiotics, and the remaining 12.5% isolates were resistant to more than two antibiotics; 95% confidence interval (0%-35.4%). The prevalence of resistant Salmonella spp. bacteria in chicken digestion tract in broiler farms in Subang District was high, so integrated control program to reduce antimicrobial resistance problem in broiler farm are greatly needed.