Articles

Found 27 Documents
Search

Keefektifan ekstrak daun kecubung (Datura metel L.) dalam menghambat penetasan dan siklus hidup Aedes aegypti L. Martini, Martini; Astriana, Novi; Yuliawati, Sri; Hestiningsih, Retno; Purwantisari, Susiana
Jurnal Entomologi Indonesia Vol 15, No 1 (2018): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.1.50

Abstract

Kecubung (Datura metel L.) merupakan jenis tanaman perdu yang mempunyai batang kayu, keras, dan tebal. Daun kecubung mengandung senyawa kimia alkaloid, saponin, flavonoida, dan fenol. Dilihat dari kandungan kimianya daun kecubung memiliki potensi sebagai insektisida nabati yang dapat menggantikan penggunaan insektisida sintetik. Penggunaan insektisida sintetik dalam mengendalikan populasi Aedes aegypti L. telah menimbulkan dampak negatif, diantaranya adalah polusi lingkungan, masalah kesehatan masyarakat, dan resistensi vektor. Tujuan penelitian ini adalah untuk mengetahui keefektifan ekstrak daun kecubung dalam menghambat penetasan telur dan  siklus hidup Ae. aegypti. Jenis penelitian ini adalah true experiment dengan 4 kali pengulangan dan perlakuan 6 konsentrasi, yaitu 125, 250, 500, 750, 1.000, dan 1.250 ppm. Subyek penelitian adalah telur Ae. aegypti fertil. Setiap unit perlakuan ditempatkan 25 telur sehingga jumlah total telur yang dibutuhkan adalah 800 butir telur nyamuk. Metode yang digunakan untuk ekstraksi adalah maserasi. Hasil analisis probit menunjukkan aktivitas insektisida ekstrak daun kecubung dengan nilai LC50 sebesar 199,340 ppm dan nilai LC90 sebesar 749,080 ppm. Hasil uji ANOVA menunjukkan terdapat perbedaan rata-rata jumlah telur yang tidak menetas akibat paparan ekstrak daun kecubung (P = 0,001). Persentase kegagalan penetasan telur Ae. aegypti paling rendah pada konsentrasi 125 ppm, yaitu sebesar 41% dan yang paling tinggi pada konsentrasi 1250 ppm, yaitu sebesar 98%. Daya hidup larva, pupa, dan nyamuk paling tinggi pada konsentrasi 125 ppm, yaitu masing-masing sebesar 49,18%; 55,17%, dan 43,75%. Sebagai kesimpulan, ekstrak daun kecubung memiliki potensi sebagai insektisida nabati terhadap telur Ae. aegypti.
HUBUNGAN HIGIENE SANITASI DENGAN KUALITAS MIKROBIOLOGIS PADA MAKANAN GADO-GADO DI KECAMATAN TEMBALANG KOTA SEMARANG Yuniatun, Tuti; Martini, Martini; Purwantisari, Susiana; Yuliawati, Sri
Jurnal Kesehatan Masyarakat (e-Journal) Vol 5, No 4 (2017): Jurnal Kesehatan Masyarakat (E-Journal)
Publisher : Fakultas Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gado-gado is one of traditional foods from Indonesia which sold in Tembalang District. Gado-gado is made from various vegetables mixed with peanut sauce, served at temperature 5-60oC which is the "temperature danger zone" for any food. This situation makes gado-gado such a watery yet not-so-hot food when its served, so has a potency to be contaminated by microbes and fungis. The purpose of this research is to understand correlation between hygiene sanitation and microbiological quality of gado-gado in Tembalang District, Semarang City. This research is observational research using cross sectional design and consists of three variables, namely sellers health condition, sellers hygienic practice, and places sanitation. The samples of this research are 36 gado-gado and respondents of this research are 36 gado-gado sellers which is observed their hygienic sanitation while processing gado-gado. Data were analyzed by frequency distribution and bivariate analysis using chi square. The results showed that sellers health condition categorized as good condition (88,9%); sellers hygienic practice categorized as good condition (33,3%); and places sanitation categorized as good condition (22,2%). There was statistically significant correlation between sellers hygienic practice (p=0,008) and places sanitation (p=0,028) with gado-gado microbiological quality. While there is no corrrelation between sellers health condition (p>0,999) with gado-gado microbiological quality.Gado-gado sellers should pay attention to their personal hygiene and the places sanitation when processing gado-gado, to improve gado-gado microbiological quality.
GAMBARAN SANITASI TEMPAT BERDAGANG WARUNG PENYET DI KECAMATAN TEMBALANG Wasisto, Barkah Haryo; Martini, Martini; Yuliawati, Sri; Purwantisari, Susiana
Jurnal Kesehatan Masyarakat (e-Journal) Vol 5, No 1 (2017): Jurnal Kesehatan Masyarakat (e-Journal)
Publisher : Fakultas Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

District Tembalang is one of the sub-district in the city of Semarang with many colleges so that there are many penyet restaurant. The purpose of this study was to determine the factors that influence the contamination of Salmonella sp on a vegetables penyet in District Tembalang, Semarang City. This study is observational analytical survey method using cross sectional design. These samples included 40 penyet restaurant observed water and vegetables fresh vegetables supplied to consumers. Data were analyzed by frequency distribution and bivariate with chi square test. The results showed the storage of vegetables vegetables using plastic bags (50%); the basket (27.5%) and sacks (22.5%). Sanitation trade places category is good (57.5%). There is a statistically significant relationship between sanitation trade places (p = 0.006) with Salmonella sp contamination in fresh vegetables. Traders are paying less attention sanitation and personal hygiene as well as a sanitation vegetables can contaminate vegetables in the vegetable vegetables Salmonella sp. Penyet restaurant traders should begin to attention about hygiene and sanitation where trade, personal hygiene when to trade, and improve the way the vegetable washing vegetables to reduce the risk of contamination of Salmonella sp.
ISOLATION AND IDENTIFICATION OF MOLD CONTAMINANTS ON MUSHROOM GROWING MEDIUM (BAG LOG) AND THEIR CELLULOLYTIC PERFORMANCE TEST Handayani, Tatik; Purwantisari, Susiana
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 2 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.241 KB)

Abstract

Mushrooms naturally grows on logs that have experienced weathering. Raw materials of mushroom growth media containing plenty of wild microbes, especially wild mold on sawdust media. Sterilization conducted did not prevent the occurrence of contamination. This study aim was to determine the types of contaminant molds in cultivated mushroom growth media (bag logs), as well as determine their cellulolytic performance. Mushroom growth media (bag log) samples were taken from three mushroom cultivation locations. Mold isolates obtained were identified by their macroscopic and microscopic characteristics. Test the mold cellulolytic performance was conducted by measuring the ratio of hydrolysis zone with the diameter of mold on CMC (Carboxy Methyl Cellullose) media. The isolation and identification results of contaminant molds consisted of mold isolates which could be grouped into 7 genus which were Aspergillus, Penicillium, Paecillomyces, Trichoderma, Rhizopus, Fusarium and Syncephalastrum. The cellulolytic performances of mold on CMC media showed that Aspergillus flavus has the greatest cellulolytic ability.
THE POTENTIAL TEST OF FUNGAL ANTAGONIST TRICHODERMA VIRIDE TO INHIBIT THE GROWTH OF PATHOGENIC FUNGI FUSARIUM MONILIFORME AND ALTERNARIA SOLANI IN-VITRO Purwantisari, Susiana; Evendi, Agus
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 3 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.695 KB)

Abstract

Fusarium moniliforme and Alternaria solani are two types of mold which often cause the diseases of cultivated plants. Fusarium moniliforme causes the ear rot disease on corn and the wilt disease of Solanaceae family. Whereas the pathogenic fungus Alternaria solani causes an early bright disease on the onion and potato. This aim of this study was to determine the ability of fungal antagonist Trichoderma viride in inhibiting the growth of Fusarium moniliforme and Alternaria solani in vitro. The growth inhibition ability test were conducted on dual cultures by growing the fungal antagonists with pathogenic fungi in Petri dish containing potato dextrose agar media face-to-face in a distance of 3 cm. Percentages of the growth inhibiting were observed every day in 7 days incubation. The results showed that the fungal antagonist T. viride exhibited the highest inhibition on F. moniliforme in 3 days incubation period which was 63.07 %. Yet the highest inhibition against A. solani was in 2 days incubation period which was 57.35 %. T. viride growth continued to increase since the first day until the seventh day incubation period but contrarily the growth of both pathogenic fungi underwent inhibition. This suggested that T. viride was potential as a biological control agent of F. moniliforme and A. solani growth and have a potency as an active bio fungicide ingredient. Keywords: Dual culture; percentage inhibiting; Trichoderma viride; Alternaria solani; Fusarium moniliforme
PRODUKSI KITOSAN SECARA ENZIMATIK OLEH BACILLUS FIRMUS E65 UNTUK PENGENDALIAN PENYAKIT ANTRAKNOSA PADA BUAH MANGGA (MANGIFERA INDICA L.) Mulyaningtyas, Dwina; Purwantisari, Susiana; Kusdiyantini, Endang; Suryadi, Yadi
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Anthracnose is postharvest disease on mango caused by fungi Colletotrichum gloesporioides. Anthracnose can be inhibited development by chitosan as a preservative anti-fungal for fruit. Effectiveness of chitosan can be increased by enzymatic hydrolyze method to obtain a low molecular weight chitosan. Low molecular weight chitosan can be reducing the particle size into nano chitosan. This study aims to obtain the low molecular weight of chitosan by hydrolyzing the commercial chitosan enzymatically using B. firmus E65, the production of nano chitosan with ionic gelation method and to determine the volume ratio of low molecular weight of chitosan and natrium tripolifosfat (NaTPP) to conduct in vitro and in vivo inhibition on the growth of fungi C. gloeosporioides. The method consist of the production of chitinase from B. firmus E65, the production chitinase of low molecular weight chitosan, ionic gelation, in vitro and in vivo nanochitosan bioassay test on the growth of C. gloeosporioides. The research result showed that the activity of  the crude extract of chitinase is 0.05 U/mL and the purity is 0.07 U/mL. The spesific activity value of crude extract is 0.44 U/mg, and the purity is 0.82 U/mg. The purity level of crude extract is increased 0.83 times from 1 to 1.83 times after purification process. Molecular weight of enzimatically hydrolyzed chitosan is  511.850 Kda. In vitro and in vivo bioassay showed the best result on the growth of C. gloeosporioides are 90 and 91% is obtained in the combination 3:1 of the low molecular weight chitosan and NaTTP. This comparison showed that the size of nano chitosan is 228.74 and the polidispesity index is 0.884. Keywords : Chitinase, chitosan, B, firmus E65, antrachnose, C. gloesporioides.
PRODUKSI ENZIM INULINASE PICHIA MANSHURICA DUCC-Y015 DENGAN PENAMBAHAN SUBSTRAT TEPUNG BENGKOANG (PASCHYRHIZUS EROSUS) Rofiqoh, Adzar; Wijanarka, W; Purwantisari, Susiana
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bengkoang (Pachyrhizus erosus) tubers has a high inulin content. Inulin bengkoang flour can be used as substrate to produce inulinase enzyme. The inulinase enzyme can be produced by Pichia manshurica DUCC-Y015. This research aims to determine the ability of Pichia mansurica DUCC-Y015 in producing inulinase enzyme with the addition of several variations of substrate concentration of bengkoang flour in its production medium. Determination of inulinase activity was done by DNS method. This research used a Completely Randomized Design (RAL) with 4 treatments: B0 (control), B1 (1 g bengkoang flour), B2 (3 g bengkoang flour) and B3 (5 g bengkoang flour). Each treatment was repeated 3 times. The inulinase activity of each treatment was 0.029 IU/mL, 0.033 IU/mL, 0.053 IU/mL and 0.015 IU/mL. The addition of variation substrate concentration bengkoang flour in the production medium did not affect the inulinase activity of Pichia manshurica DUCC-Y015Kata Kunci: Pachyrhizus erosus, inulinase, Pichia manshurica DUCC-Y015.
UJI POTENSI EKSTRAK DAUN SUREN DALAM MENGHAMBAT PERTUMBUHAN JAMUR COLLETOTRICHUM CAPCISI SECARA IN VITRO Andriyani, Fiva; Purwantisari, Susiana
Jurnal Akademika Biologi Vol. 8 No. 1 Januari 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun suren terhadap penghambatan pertumbuhan jamur patogen Colletotrichum capsici. Penelitian dilakukan di Laboratorium Pengamatan Hama dan Penyakit Tanaman (PHP) Kedu Temanggung dan Laboratorium Terpadu Universitas Diponegoro, Semarang. Ekstrak daun suren dengan konsentrasi 0%, 10%, 20%, dan 30% diujikan terhadap pertumbuhan jamur C.capsici secara in vitro. Pengamatan penghambatan pertumbuhan jamur C.capsici dilakukan pada hari ke-5, ke-10, dan ke-15 setelah inokulasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan setiap perlakuan masing-masing dilakukan tiga pengulangan. Data yang diperoleh ke dianalisis dengan ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun suren mampu menghambat pertumbuhan jamur C.capsici. Ekstrak daun suren dengan konsentrasi 30% menunjukkan penghambatan tertinggi dibandingkan dengan konsentrasi yang lain terhadap pertumbuhan jamur C. capsici. 
KEMAMPUAN ANTAGONISME PSEUDOMONAS SP. DAN PENICILLIUM SP. TERHADAP CERCOSPORA NICOTIANAE IN VITRO Iskandar putra, Muhammad Bismar; Purwantisari, Susiana
Jurnal Akademika Biologi Vol. 7 No.3 Juli 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antagonisme merupakan mekanisme suatu mikroorganisme dalam melakukan penghambatan terhadap organisme lainnya. Mekanisme penghambatan ini dapat dilakukan dengan adanya produksi senyawa antibiotik untuk menghambat pertumbuhan organisme lainnya. Pada penelitian ini, pengujian kemampuan antagonisme memanfaatkan Pseudomonas sp. dan Penicillium sp. yang diujikan pada jamur patogen Cercospora nicotianae. Penelitian ini bertujuan untuk mengetahui kemampuan antagonisme dari Pseudomonas sp. dan Penicillium sp. dalam mekanisme penghambatannya terhadap jamur patik Cercospora nicotianae. Parameter yang diamati dalam penelitian ini yaitu pengamatan mikroskopis bentuk morfologi hasil isolasi Cercospora nicotianae, pengamatan mikroskopis bentuk morfologi hasil isolasi Penicillium sp., inokulasi bakteri Pseudomonas sp., dan pengujian antagonisme Pseudomonas sp., Penicillium sp. terhadap Cercospora nicotianae. Hasil pengamatan secara mikroskopis pada isolasi Cercospora nicotianae memiliki morfologi konidia bersekat dan tidak berwarna, konidiofor meruncing pada bagian ujungnya. Pengamatan mikroskopis pada isolasi Penicillium sp. menunjukkan kenampakan mikroskopis seperti sapu, terdapat konidia, phialid, dan konidiofor, konidiumnya berbentuk rantai. Berdasarkan hasil penelitian ini, pertumbuhan miselium Cercospora nicotianae mulai terlihat pada masa inkubasi hari ke-3, sedangkan pada Penicillium sp. optimal pertumbuhannya terlihat pada masa inkubasi hari ke-7. Hasil pengujian antagonisme Pseudomonas sp. dan Penicillium sp. setelah diinkubasikan selama 4 hari pada media yang berisi jamur patogen Cercospora nicotianae menunjukkan pada Pseudomonas 1, persentase daya hambatnya 43,4% dan pada Pseudomonas 2, persentase daya hambatnya 43,4%. Pengujian pada Penicillium sp. 1 menunjukkan persentase daya hambat sebesar 58,6% dan Penicillium sp. 2 persentase daya hambatnya 60,8%. Hal ini menunjukkan bahwa Penicillium sp. memiliki kemampuan antagonisme yang lebih kuat dibandingkan Pseudomonas sp.    
PENINGKATAN PERTUMBUHAN DAN HASIL PANEN KENTANG OLEH APLIKASI BIOFUNGISIDA TRICHO POWDER PRODUK LOKAL TEMANGGUNG Purwantisari, Susiana; Parman, Sarjana; Sitepu, Harum
Jurnal Akademika Biologi Vol. 7 No. 4 Oktober 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penyakit hawar daun oleh jamur patogen Phytophthora infestans merupakan penyakit utamapada tanaman kentang yang dapat menurunkan produksi hingga 100%. Penggunaan agensia hayati Trichoderma spp. sebagai penginduksi ketahanan tanaman budidaya terhadap serangan patogen penyebab penyakit tanaman telah lama terbukti sebagai metode yang sangat efektif dilakukan. Tujuan penelitian ini adalah mengevaluasi penggunaan biofungisida Tricho powder berbahan aktif jamur antagonis Trichoderma harzianum produk petani lokal untuk menginduksi ketahanan tanaman kentang sehingga mampu meningkatkan hasil panennya. Metode penelitian menggunakan penelitian eksperimental dengan pola penelitian rancangan acak lengkap dengan 5 perlakuan. Penelitian dilakukan di lahan demplot pertanaman tanaman kentang di Desa Kledung Temanggung dengan ketinggian 1350 meter di atas permukaan laut. Hasil penelitian menunjukkan bahwa aplikasi biofungisida Tricho powder dapat meningkatkan pertumbuhan dan hasil panen tanaman kentang.