Articles

KERAGAAN KARAKTER PEMBUNGAAN KUANTITATIF DAN PROFIL METABOLOMIK BAWANG MERAH (ALLIUM CEPA VAR. AGGREGATUM) YANG DIINDUKSI DENGAN PERLAKUAN VERNALISASI Marlin; Maharijaya, Awang; Sobir; Purwito, Agus
Jurnal Hortikultura Indonesia Vol. 9 No. 3 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.116 KB) | DOI: 10.29244/jhi.9.3.197-205

Abstract

Proses pembungaan pada bawang merah (Allium cepa var. aggregatum) sangat dipengaruhi faktor internal dan eksternal tanaman. Induksi pembungaan dengan perlakuan vernalisasi dilakukan untuk mendorong inisiasi pembentukan bunga bawang merah. Penelitian bertujuan untuk menganalisis karakter kuantitatif pembungaan dan profil metabolomik bawang merah yang diberikan perlakuan vernalisasi pada stadia perkembangan umbi. Vernalisasi dilakukan pada suhu 8 0C selama 6 minggu terdiri atas empat taraf, yaitu umbi tanpa vernalisasi (S0), vernalisasi pada stadia embrio (S1), vernalisasi pada stadia tumbuh tunas 1 cm (S2), dan vernalisasi pada stadia tumbuh tunas 2 cm (S3). Perlakuan vernalisasi pada stadia awal pertumbuhan umbi mampu meningkatkan karakter jumlah anakan, jumlah umbel, diameter umbel, dan persen berbunga bawang merah dibandingkan vernalisasi pada stadia lainnya. Stadia awal pertumbuhan umbi merupakan stadia terbaik dalam menerima perlakuan vernalisasi. Semakin besar jumlah umbel dan diameter umbel semakin meningkatkan persen berbunga bawang merah. Karakter jumlah umbel dan diameter umbel dapat digunakan sebagai karakter seleksi untuk peubah persen berbunga bawang merah. Analisis metabolomik berhasil mengidentifikasi 104 metabolit spesifik dan mengelompokkan bawang merah menjadi 3 kelompok stadia. Vernalisasi pada stadia awal perkembangan umbi (stadia embrio dan tunas 1 cm) menunjukkan adanya perubahan fase reproduktif, yang dicirikan dengan adanya senyawa phytol dan 2 propanone. Kata kunci: metabolomik, analisis pengelompokan hierarkis, induksi pembungaan, korelasi
EMBRIOGENESIS DAN DESIKASI EMBRIO SOMATIK JERUK KEPROK BATU 55 (CITRUS RETICULATA BLANCO.) UNTUK MENINGKATKAN FREKUENSI PERKECAMBAHAN Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol. 8 No. 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.578 KB) | DOI: 10.29244/jhi.8.2.79-87

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ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan
EVALUASI KERAGAMAN GENETIK MUTAN HARAPAN GENERASI MV1 JERUK KEPROK SOE (CITRUS RETICULATA BLANCO) BERDASARKAN PENANDA MORFOLOGI DAN ISSR Husain, Indriati; Purwito, Agus; Husni, Ali; Mutaqin, Kikin H.; Susanto, Slamet
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.675 KB) | DOI: 10.29244/jhi.7.2.102-110

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ABSTRACTMandarin?s SoE is national variety originated from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. Genetic diversity detection can be based on morphological and ISSR markers. The aim of this research was to obtain information on the genetic diversity on putative mutants mandarin SoE induced by gamma ray irradiation based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with 5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals samples obtained from gamma irradiation, based both morphological and ISSR markers, was different from individual's genetic make up before irradiation.Keywords: cluster, gamma ray, genetic distance, genetic diversitys, similarity ABSTRAKJeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma
PERBANYAKAN IN VITRO DENDROBIUM INDONESIA RAYA ‘INA’ MELALUI EMBRIOGENESIS SOMATIK BERBASIS SISTEM BIOREAKTOR Rachmawati, Fitri; Wiendi, Ni Made Armini; Mattjik, Nurhajati Ansori; Purwito, Agus; Winarto, dan Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.183 KB) | DOI: 10.24831/jai.v44i3.12816

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ABSTRACTAn effective and efficient in vitro propagation system has important roles in preparing and producing high quality-seedlings of Dendrobium for commercial scale. The objective of this research was to establish an effective and efficient embryogenic callus (EC) proliferation method using bioreactor system and regeneration EC into plantlet for producing high quality seedlings of Dendrobium Indonesia Raya ?Ina?. Differences in callus densities (5, 10, 15, and 20 g callus in 250 mL medium), aeration levels (2.5, 5.0, and 10.0 O2 volume  per  medium volume per minute; vvm), and regeneration media half-strength MS and 2 g L-1 NPK (32:10:10) combinated by 0.00, 0.05 mg L-1 BA, 150  mL L-1 coconut water and their combinations were tested in this experiment. The experiments were arranged using randomized completely block design (RCBD) with three replications for EC proliferation and randomized completely desaign (RBD) for EC regeneration. The results showed that combination of  aeration at 2.5 vvm and 10 g of EC was the most suitable aeration level and callus density for proliferation of EC in the 500 ml airlift bioreactor with 6.85 multiplication rate, 92.5% EC formation, and malformed callus morphology as low as 6.1%. The highest somatic embryos (SEs) formation was 87.7% with 44.5 SEs per clump and 92.1% SEs germination with 41.0 germinated-SEs per clump, 85.1% normal germinated-SEs, and whereas the best performance of plantlet was obtained from 1/2 MS + 0.05 mg L-1 BA semi solid medium. Plantlets were successfully acclimatized using Cycas rumphii medium with high survival rate (91.6%). Keywords: aerations, callus densities, germination, media, somatic embryos
RADIOSENSITIVITAS DAN SELEKSI MUTAN PUTATIF JERUK KEPROK GARUT (CITRUS RETICULATA L.) BERDASARKAN PENANDA MORFOLOGI ,, Karyanti; Purwito, Agus; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.529 KB) | DOI: 10.24831/jai.v43i2.10417

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ABSTRACTMandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%.Keywords: gamma irradiation, germination, lethal dosis 50 (LD50), maturation, somatic embryo
PEMBENTUKAN TANAMAN CABAI HAPLOID MELALUI INDUKSI GINOGENESIS DENGAN MENGGUNAKAN SERBUK SARI YANG DIRADIASI SINAR GAMMA Suharsono, ,; Alwi, Muhammad; Purwito, Agus
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.329 KB) | DOI: 10.24831/jai.v37i2.1404

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The objective of this research was to obtain haploid hot pepper plants by inducing gynogenesis with g-ray irradiated pollen for pollination. Fruits resulted from pollination by irradiated pollen was used as the explants of the in vitro culture in solid MS medium containing BAP, IAA and GA3. The resulted plants then cultivated in MS0 medium. The result of the research showed that hot pepper immature embryo could grow and developed into whole plant when the age of this embryo was 9 days after pollination or more. The haploid hot pepper plants can be obtained by pollinating the pistil with 10 Gy irradiated pollen. The MS medium containing BAP 0.3 + IAA 0.2 + GA3 0.5 mg/l and BAP 0.4 + IAA 0.1 + GA3 0.5 mg/l supported well the development of immature embryo into whole plants. The growth of haploid hot pepper plants was slower than that of diploid ones.   Key words:  Hot pepper, haploid plant, gynogenesis, g-ray.
INDUKSI TETRAPLOID TUNAS PUCUK JERUK SIAM SIMADU (CITRUS NOBILIS LOUR) MENGGUNAKAN KOLKISIN SECARA IN VITRO Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.427 KB) | DOI: 10.24831/jai.v43i1.9593

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Seedless fruit is one of the criteria (necessary) to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is a diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines.The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0, 0.1, 0.2, and 0.3%) for 3 hours. The results showed that the high concentration of 0.3% reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine.The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplast per pair of guard cells. Compared to those of control, leaves with 0.1% colchicine had lower stomatal density, and larger stomatal size. It appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.Keywords:chloroplasts, doubling chromosomes, stomatal size, stomatal density
RESISTANCE EVALUATION ON POPULATIONS OF CROSSES BETWEEN TRANSGENIC POTATO KATAHDIN RB AND NON-TRANSGENIC ATLANTIC AND GRANOLA TO LATE BLIGHT (Phytophthora infestans) IN CONFINED FIELD TRIAL Ambarwati, Alberta Dinar; Herman, Muhammad; Purwito, Agus; Sumaraw, Sientje Mandang; Aswidinnoor, Hajrial
Indonesian Journal of Agricultural Science Vol 12, No 1 (2011): April 2011
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Late blight resistance gene (RB gene) isolated from Solanum bulbocastanum, is a broad resistance gene against all races of Phytophthora infestans. The gene was transformed into Katah-din event SP904 and SP951 using Agrobacterium tumefaciens and these transgenic plants have been crossed with susceptible potato cultivars Atlantic and Granola. Populations of the crosses have been molecularly characterized for the integration of the RB transgene. The study aimed to evaluate the resistance of the populations of crosses between transgenic Katahdin RB  and susceptible non-transgenic parents (Atlantic and Granola) to late blight in a confined field trial at Pasir Sarongge, Cianjur, West Java. A total of 84 clones originated from four popula-tions were evaluated for resistance to late blight. These included 22 clones of Atlantic x transgenic Katahdin SP904, 16 clones of Atlantic x transgenic Katahdin SP951, 19 clones of Granola x transgenic Katahdin SP904, and 27 clones of Granola x transgenic Katahdin SP951. Observations of the late blight infection were conducted when late blight symptoms were detected, i.e. at 56, 60, 63, 70, and 77 days after planting (DAP). The result showed there were high variations in the resistance level of all the 84 clones tested. Clones of crosses between susceptible parents (Atlantic or Granola) and resistant parents (transgenic Katahdin SP904 or Katahdin SP951) showed a similar pattern based on the area under disease progress curve (AUDPC) value, i.e. 377.2 greater than the AUDPC of the resistant parents (180.1), but smaller than that of the susceptible parents (670.7). Observation at 77 DAP resulted four resistant potato clones having resistance score of 7.0-7.6, higher than the transgenic parents Katahdin SP904 (4.6) and Katahdin SP951 (6.8), i.e. clone B8 (Atlantic x transgenic Katahdin SP951) with resistance score of 7.6 and clones B26 (Atlantic x transgenic Katahdin SP951), C183 (Granola x transgenic Katahdin SP904), and D89 (Granola x transgenic Katahdin SP951) with resistance score of 7. These four transgenic potato resistant clones need to be further developed as promising potato clones to late blight.
DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica) Ibrahim, Meynarti Sari Dewi; Hartati, Rr. Sri; Rubiyo, Rubiyo; Purwito, Agus; Sudarsono, Sudarsono
Indonesian Journal of Agricultural Science Vol 14, No 2 (2013): October 2013
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.
Efficacy of RB gene in transgenic potato Katahdin SP904 and SP951 to West Java isolates of Phytophthora infestans Ambarwati, A. Dinar; Sumaraw, S. M.; Purwito, Agus; Herman, M.; Suryaningsih, E.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

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Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant disease. Potato yield losses due to this disease ranged from 47-100%. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum. RB gene has been integrated into cultivated potato Katahdin using Agrobacterium-mediated transfor-mation, and showed durable and broad spectrum resistance either in laboratory assay or in confined field trial. Evaluation of transgenic Katahdin SP904 and SP951 was conducted to verify whether the RB gene with broad spectrum to all known races of P. infestans in the United States and in Toluca, Mexico was also effective against P. infestans isolates in Indonesia. Efficacy of RB gene was evaluated for foliar and tuber resistance to West Java isolates. Transgenic Katahdin were more resistant in foliar than non transgenic plants, at 14 days after inoculation. Diseases intensity of transgenic Katahdin SP904 and SP951 were 19.8-43.8%, whereas non transgenic Katahdin, Granola, and Atlantic were 46.9-100%. In contrast to the foliar resistance phenotype, RB-containing tubers in transgenic Katahdin did not exhibit increased resistance to Lembang, Pangalengan and Galunggung isolates. Tubers of transgenic Katahdin SP904, SP951, and non transgenic Katahdin showed lesion volume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene in transgenic Katahdin showed efficacy against late blight P. infestans in foliar, but did not showed efficacy in tuber. Transgenic Katahdin RB thus providing a potential source of resistance for breeding programs.
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Sobir , Suharsono , Supenti, , A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Alberta Dinar Ambarwati Ali Husni Anas D Susila Andri Ernawati ANTONIUS SUWANTO Arifin Noor Sugiharto Asep Setiawan Atra Romeida Awang Maharijaya Budi Marwoto Budi Winarto Catur Herison DEWI SUKMA Didy Soepandi, Didy DIDY SOPANDIE Dini Dinarti Diny Dinarti Dwi Andreas Santosa E. Suryaningsih Endang Suhendang Enny Sudarmonowati ERNI SUMINAR Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti Frihadian, Rd. Zainal G. A. Wattimena G. A. Wattimenal GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena, Gustav Adolf H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri I Made Arisudana Putra, I Made Iis Rahmawati Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono IRDIKA MANSUR Ismail Maskromo J. M. Pasaribu JULIUS DWI NUGROHO Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H. Mutaqin, Kikin H. laela Sari, laela M. Herman M. Machmud Maharijaya, dan Awang Marlin Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Mia Kosmiatin Muhamad Herman Muhammad Alwi MUHAMMAD HERMAN Muhammad Mahmud Muhammad Syukur Ni Made Armini Wiendi Noor Farid Nurhajati Ansori Mattjik Nurhayati Ansori Mattjik Nurul Khumaida Prayogi, Mohamad Prima Muklisa RAGAPADMI PURNAMANINGSIH Ravenska, Nidya Rd. Selvy Handayani Retno Prihatini Reza Ramdan Rivai Ridho Kurniati ROEDHY POERWANTO Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rubiyo Rubiyo S M Sumaraw, S M S. M. Sumaraow, S. M. S. M. Sumaraw SIENTJE MANDANG SUMARAW Slamet Susanto Sobir Sri Rianawati SUDARSONO SUDARSONO Sudarsono, dan SUDIRMAN YAHYA Surjono Hadi Sutjahjo SURJONO HADI SUTJAHJO Suryanah Suryanah Susiyanti ., Susiyanti Suskandari Kartikaningrum Syarifah Iis Aisyah Tri Muji Ermayanti WARID, WARID Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno