Bambang S. Purwoko
Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor, Jl. Raya Darmaga, Bogor 16680

Published : 42 Documents
Articles

ELIMINASI ONION YELLOW DWARF VIRUS MELALUI KULTUR MERISTEM TIP PADA BAWANG MERAH (ALLIUM ASCALONICUM L.) ,, Aqlima; Purwoko, Bambang S.; Hidayat, Sri Hendrastuti; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol. 8 No. 1 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.098 KB) | DOI: 10.29244/jhi.8.1.22-30

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ABSTRACTMeristem tip culture is culture of isolated meristem with 1-2 leaf primordia on suitable medium. This method is generally used to obtain free virus plant. Optimation of plant growth regulators (PGRs) was done to accelerate explant growth without callus formation and to avoid somaclonal variation in meristem tip culture. The aims of this study were to achieve the best combination of PGR for meristem tip growth and to evaluate meristem tip culture potential for Onion yellow dwarf virus (OYDV) elimination in shallot. This study used combination of PGRs 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) with or without 0.1 mg L-1 IAA and medium without PGR. This research consisted of two experiments conducted separately. In experiment I, cv. Bima Brebes was used and experiment II cv. Tiron was used. Each experiment was arranged in completely randomized block design with single factor (PGR combination) that has 8 combination levels and 3 replications. The result showed that medium without PGR was the most efficient for meristem tip growth. Primary shoot was growing without callus formation. RT-PCR analysis showed that all of the tested samples were still infected by OYDV. Meristem tip culture method did not eliminate OYDV in both cultivars.Keywords: Auxin, cytokinin, GA3, OYDV, RT-PCRABSTRAKKultur meristem tip merupakan kultur meristem yang diisolasi 1-2 primordia daun dan pada media yang sesuai. Metode ini umum digunakan untuk mendapatkan tanaman bebas virus. Optimasi terhadap zat pengatur tumbuh (ZPT) dilakukan untuk mempercepat pertumbuhan eksplan tanpa disertai pembentukan kalus untuk menghindari terjadinya variasi somaklonal pada kultur meristem tip. Penelitian ini bertujuan untuk mendapatkan kombinasi ZPT terbaik bagi pertumbuhan meristem tip dan untuk mengevaluasi potensi kultur meristem tip dalam mengeliminasi virus Onion yellow dwarf virus (OYDV) pada tanaman bawang merah. Penelitian ini menggunakan 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) dengan penambahan atau tanpa 0.1 mg L-1 IAA serta media tanpa ZPT. Penelitian ini terdiri atas 2 percobaan terpisah. Percobaan 1 menggunakan cv. Bima Brebes dan Percobaan 2 menggunakan cv. Tiron. Masing-masing percobaan disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan 1 faktor, yaitu kombinasi ZPT yang terdiri atas 8 taraf kombinasi dan 3 ulangan. Hasil yang didapat menunjukkan bahwa media tanpa penambahan ZPTmerupakan media yang paling efisien untuk pertumbuhan tunas meristem tip. Tunas utama tumbuh tanpa disertai pembentukan kalus. Hasil analisis RT-PCR menunjukkan bahwa seluruh sampel yang dideteksi masih terinfeksi OYDV. Metode kultur meristem tip belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.Kata kunci: Auksin, GA3, OYDV, RT-PCR, sitokinin
INDUKSI KALUS DAN REGENERASI TIGA GENOTIPE TOMAT (SOLANUM LYCOPERSICON L.) MELALUI KULTUR ANTERA Ningsih, Ratna; Purwoko, Bambang S.; Syukur, Muhamad; Dewi, Iswari S.
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.486 KB) | DOI: 10.29244/jhi.7.2.75-82

Abstract

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others genotypes. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2% while in Tora was 2.1% and Ratna was 0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4%.Keywords: androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah tomat varietas Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2% lebih tinggi dibandingkan Tora (2.1%) dan Ratna (0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4%).Kata kunci: androgenesis, auksin, in vitro, media, sitokinin, tomat
TAKSONOMI MANGGA BUDIDAYA INDONESIA DALAM PRAKTIK Fitmawati, ,; Hartana, Alex; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.283 KB) | DOI: 10.24831/jai.v37i2.1405

Abstract

The classification  of  cultivated plants should meet two approaches namely the botanical (classifying based on essential systematic plant characters) and the practical approach (clustering based on the analysis of commercial properties). This study analyzed taxonomy of mango cultivars grown in Indonesia based on morphology and agronomy characters. It obtained 84 recognizable cultivars.They are grouped into eigth main cultivar-groups (e.g. Berem, Madu, Gedong, Golek, Bapang, Arumanis, Kepodang, and Kebo) and eigthteen cultivar-groups. The 'Lalijiwo' cultivars synonym with 'Thaber', 'Tabar', 'Gurih' whereas 'Arummanis' synonim with 'Gadung'. Meanwhile, the cultivar 'Kates277' which is a member of Golek cultivar main group is homonim with the cultivar 'Kates' in Arummanis cultivars main group. Furthermore, the cultivar Nanas93 (one of Madu main group cultivar) that different with  the cultivar 'Nanas71' which is categorized as member of Bapang main cultivar group.   Key words: Taxonomy of cultivated plant, Indonesian mango, morphology, agronomy characters
KERAGAMAN GENETIK DAN KARAKTER AGRONOMI GALUR HAPLOID GANDA PADI GOGO DENGAN SIFAT-SIFAT TIPE BARU HASIL KULTUR ANTERA Herawati, Reny; Purwoko, Bambang S.; Dewi, Iswari S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.979 KB) | DOI: 10.24831/jai.v37i2.1399

Abstract

The development  of new plant type of upland rice in relatively short time can be done by using anther culture technique. The technique has been recognized as a rapid and efficient technology for plant improvement. Plant materials  used in this research were 320 s doubled haploid lines derived from anther culture and their 4 parents   namely Fatmawati, SGJT-28, SGJT-36 and Way Rarem. Observation was conducted on  plant height, number of productive tillers, days of flowering and maturity, length of panicle, number of grains per panicle,  number of filled grains per panicle, percentage of unfilled grains, weight of 1000 grains and weight of grains per hill. The result showed that there were broad variations in the agronomic characters of doubled haploid lines. There were 58 upland rice lines can be obtained with new plant type characters (NPT).  Several lines showed superior characters. Line P6-105, P3-134, P3-135, P3-175 had productive tiller more than that of parental, P3-160, P3-196, P6-274  had long panicle, number of grain per panicle and low percentage of unfilled grain,  P3-135, P6-271, P6-274, and P6-276 had weight of grains per hill. These lines had potential as new plant type of upland rice. Length of panicle, number of grains per panicle,  number of filled grains per panicle, and weight of grains per hill had high heritability and wide genetic variability.   Key words: Genetic variability,  doubled haploid, anther culture, upland rice  
EVALUASI GALUR HAPLOID GANDA PELESTARI HASIL KULTUR ANTERA UNTUK PERAKITAN GALUR MANDUL JANTAN PADA PADI Rumanti, Indrastuti A.; Dewi, Iswari S.; Purwoko, Bambang S.; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.008 KB) | DOI: 10.24831/jai.v37i1.1387

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Hybrid rice has been proven to be the key factor to increase food supply in highly populated countries such as China and India. The success has encouraged the government of Indonesia to intensify research and development on hybrid rice using cytoplasmic-genetic male sterility system. The use of good male sterile line is a prerequisite for commercial seed production of hybrid rice.  The objective of this research was to evaluate several F1 plants to develop completely sterile cytoplasmic male sterile line (CMS) with good agronomic characters.  This research was conducted at screen house of Bogor Agricultural University during dry season (DS) of 2007.  Fourty eight F1s, derived from testcross between cytoplasmic male sterile sources with doubled-haploid (DH) maintainer lines, were evaluated in randomized complete design using three replications.  Observation was done on pollen sterility, panicle and stigma exsertion, plant height, and tillering ability.  The results showed 14 F1s were completely sterile (100 % pollen sterility).  They were A-2/H36-3-Ma, A-1/H36-3-Mb, A-2/H36-3-Mb, A-3/H36-3-Mb, A-1/H36-4-M, A-2/B1-1-Mb, A-3/B1-1-Mb, A-1/B1-2-Pa, A-2/B1-2-Pb, A-3/B2-1-M, A-1/B2-4-Pb, A-3/B4-1-Da, A-1/B4-1-Dc, and A-3/B4-1-Dc.  They also had early flowering date, averaged from 66 - 90 days after planting (DAP).  There were other 14 F1s with stigma exsertion more than 70 % and good panicle exsertion, i.e.  A-2/H36-3-Mb, A-1/H36-3-Mc, A-2/H36-3-Mc, A-1/H36-4-M, A-2/B1-2-Pa, A-2/B1-2-Pb, A-3/B2-1-Db, A-1/B2-1-Dc, A-1/B2-1-M, A-1/B2-4-Pb, A-3/B2-4-Pb, A-2/B4-1-Da, A-1/B4-1-Dc and A-3/B4-1-Dc.  However, only five F1s had all desirable characteristics as male sterile line candidates, such as 100% pollen sterility, good panicle exsertion, high stigma exsertion, semi-dwarf in plant height and good tillering ability.  They were A-1/H36-4-M, A-2/B1-2-Pb, A-1/B2-4-Pb, A-1/B4-1-Dc, A-3/B4-1-Dc.   Key words:  Doubled-haploid maintainer lines, CMS, pollen sterility, hybrid rice
EVALUASI BEBERAPA EKOTIPE JARAK PAGAR (JATROPHA CURCAS L.) UNTUK TOLERANSI CEKAMAN KEKERINGAN Lapanjang, Iskandar; Purwoko, Bambang S.; Hariyadi, ,; Budi R, Sri Wilarso; Melati, Maya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.78 KB) | DOI: 10.24831/jai.v36i3.1386

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Drought stress in crop  occurs due to imbalance between water supply and demand.  Crop responses to drought stress  depend on their ecotypes.  This study was aimed at examining the tolerance of the physic nut ecotype to drought stress in inceptisol. This study was conducted at Silviculture greenhouse, Forestry Faculty IPB, from September to December 2007. A Completely Randomized Design with two factors was used in the experiment, i.e. water content (80, 60, and 40 % of field capacity) and physic nut ecotypes (Palu, NTB, IP-A, and IP-P) The results showed that (1) drought reduced stem diameter (31.4%), root length (65.49%) and leaf area (72.73%), and decreased plant dry weight (74.83%),(2) tolerance level of the evaluated physic nuts on the drought stress were moderate tolerance (IP-1A, NTB,  Palu) and sensitive (IP- Pakuwon).   Key words:  physic nut, drought stress, tolerance
TANGGAP PERTUMBUHAN DAN HASIL CABAI (CAPSICUM ANNUUM L.) TERHADAP INOKULASI FUNGI MIKORIZA ARBUSKULA PADA TANAH ULTISOL Purnomo, D. Wasgito; Purwoko, Bambang S.; Yahya, Sudirman; Sujiprihati, Sriani; Mansur, Irdika; Amisnaipa, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.12 KB) | DOI: 10.24831/jai.v36i3.1381

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The aim of this research was to test the utilization of arbuscular mycorrhizal fungus (AMF) to improve adaptation ability of chili to aluminum (Al) stress based on growth and yield response in four genotypes of chili (Capsicum annuum L.).  The research was carried out at University Farm of IPB in Cikabayan from September 2006 to Maret 2007.  The media was Ultisol from Gajrug (Lebak, Banten) in polybag.  Four genotypes of chili, PBC 619 and Jatilaba (Al tolerant genotypes), and Cilibangi 3 and Helm (Al sensitive genotypes) were treated with and without inoculation of Gigaspora margarita, and planted further in media without Al stress (Al saturation 0.77%) and Al stress (Al saturation 60.85%).  Variables observed were degree of AMF infection, root length, plant height, shoot dry weight, number of harvested fruits, fruit length, fruit weight and weight of harvested fruits.  The results showed that inoculation of G. margarita effectively decreased negative influence of Al stress by increasing plant height, shoot dry weight, number of harvested fruit, fruit length, weight per fruit and weight of harvested fruits.  Inoculation of G. margarita to Al sensitive genotypes was more advantageous than to tolerant genotypes.  The Al sensitive genotypes were adapted to Al stress if they were inoculated with G. margarita.  In Al stress condition, inoculation of G. margarita to Al sensitive genotypes increased weight of harvested fruits up to 94.49% in Cilibangi 3 and 80.37% in Helm.   Key words: Adaptation, aluminum stress, arbuscular mycorrhizal fungus, Capsicum annuum L., Ultisol
ROLE OF POLYAMINES IN INHIBITION OF ETHYLENE BIOSYNTHESIS AND THEIR EFFECTS ON RICE ANTHER CULTURE DEVELOPMENT Dewi, Iswari S.; Purwoko, Bambang S.
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

The polyamines such as putrescine, spermidine, and sperminewere reported to increase green plant regeneration in rice antherculture. Low response of anther culture of rice sub-speciesindica may be improved with the addition of putrescine in theculture media. Four experiments were conducted to study therole of polyamines in inhibition of ethylene biosynthesis andtheir effects on rice anther culture development. Anthers oftwo subspecies of rice, indica (IR64, Krowal, Jatiluhur) andjaponica (Taipei 309) were cultured onto media supplementedwith putrescine (N6P) and without putrescine (N6). Youngpanicles containing the anthers at mid-to-late nucleatemicrospores were cold pretreated at 5 + 2°C and incubated inthe dark for 8 days before the anthers were cultured. Resultsshowed that medium without putrescine produced an earliersenescence of indica rice anther than that of japonica. Theaddition of 10-3 M putrescine into the culture media inhibitedethylene biosynthesis as anther senescence delayed, increasedthe three polyamines contents, and decreased the ACC contentas well as ACC oxydase activity in anther-derived calli. In theanther and anther-derived calli of subspecies indica, the totalpolyamines content was lower (10.14 nM g-1 anther and 8.48 nMg-1 calli) than that of subspecies japonica (12.61 nM g-1 antherand 10.16 nM g-1 calli), whereas the ethylene production washigher (32.31 nM g-1 anther and 2.48 nM g-1 calli) than thejaponica (31.68 nM g-1 anther and 1.76 nM g-1 calli). This studysuggests that application of 10-3 M putrescine in anther cultureof rice subspecies indica improves androgenesis by inhibitingearly senescence of cultured anthers and enhancing embryo orcallus formation from microspores.
PEMBENTUKAN GALUR HAPLOID GANDA PADI GOGO DENGAN SIFAT-SIFAT TIPE BARU MELALUI KULTUR ANTERA Herawati, Reny; Purwoko, Bambang S.; Khumaida, Nurul; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.735 KB) | DOI: 10.24831/jai.v36i3.1375

Abstract

The breeding of upland rice with New Plant Type characters in relatively short time can be done by using anther culture technique. The technique has been recognized as a rapid and efficient technology for crop improvement. Plant materials used in this research were F1 crossing P1 (Fatmawati x Way Rarem), P2 (Fatmawati x SGJT-28),  P3 (Fatmawati x SGJT-36), P4  (Way Rarem x Fatmawati), P5 (SGJT-28 x Fatmawati), and P6  (SGJT-36 x Fatmawati). Media for calli induction (N6) and regeneration (MS) were according to Dewi methods (2003). The results of this study indicated that P3 (Fatmawati x SGJT-36) and P6 (SGJT-36 x Fatmawati)  from resiprocal crosses gave better response in anther culture than the others crosses for their calli induction and green plant regeneration. From the six F1 crosses, 348 (53.5%) spontaneous doubled haploid (DH) pure lines were obtained, six lines from Farmawati x Way Rarem, 13 lines from Fatmawati x SGJT-28, 187 lines from Fatmawati x SGJT-36, three lines from Way Rarem x Fatmawati, five lines from SGJT-28 x Fatmawati, and 134 lines from SGJT-36 x Fatmawati.  These lines should be evaluated and characterized for their agronomical and morphological  traits.    Key words: Anther culture, doubled haploid, callus induction, green plant regeneration        
PENGARUH APLIKASI ASAM GIBERELIN (GA3) TERHADAP HASIL BENIH PADI HIBRIDA Susilawati, Pepi Nur; Surahman, Memen; Purwoko, Bambang S.; Suharsi, Tatiek K; Satoto, ,
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 17, No 2 (2014): Juli 2014
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

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Abstract

The Effect of Giberelin Acid (GA3) Application to Seed Yield of Hybrid Rice. Seed yield of rice hybrid on three line system has weakness in panicle exertion and outcrossing that cause low yield.  GA3 has been proved to increase the seed set through increasing panicle exertion and improved outcrossing. The purpose of this study was to determine the effect of GA3 application to increase seed yield of hybrid rice (F1). The study was conducted in Singamerta Experimental Farm, Banten Assesment Institute for Agricultural Technology from May to October 2013. The experiment was arranged in a split plot designed with four replications. The main plot were parental lines (cytoplasmic male sterility/CMS and restorer/R) of hybrid i.e. HIPA 8 (A1 and BP51-1), HIPA 6 (A2 and B8094), HIPA Jatim 3 (A6 and PK88) and HIPA 14 SBU (A7 and BH33d-Mr-57-1-2-2). Sub plot were the  frequency of GA3 treatments i.e. control (W0), two (W1), and three (W3) times application of GA3. GA3 dosage was 200 ppm, the best dose from the previous studies. The results showed that the GA3 applications increased plant height, stigma exertion, panicle exertion, duration of floret opening and panicle length. Two times application (W1) of  GA3  gave the best results as indicated by the highest seed yield (1429 kg/ha) that was better than three times application of GA3 that gave seed yield 1215 kg/ha and control with yield of 703 kg/ha.Keywords : GA3, hybrid rice, seed yieldABSTRAKHasil benih padi hibrida sistem tiga galur memiliki kelemahan, yaitu rendahnya eksersi malai dan tingkat penyerbukan silang,sehingga hasilnya rendah. Percobaan terdahulu menunjukkan bahwa aplikasi GA3 dapat meningkatkan hasil melalui peningkatan eksersi malai dan penyerbukan silang. Penelitian ini bertujuan untuk mengetahui pengaruh frekuensi aplikasi GA3 terhadap peningkatan hasil benih padi hibrida (F1). Penelitian dilakukan di Kebun Percobaan Singamerta, Balai Pengkajian Teknologi Pertanian (BPTP) Banten pada Mei sampai Oktober 2013. Penelitian menggunakan rancangan petak terbagi dengan empat ulangan. Petak utama yaitu tetua padi hibrida (galur mandul jantan/GMJ dan restorer) yaitu HIPA 8 (A1 dan  BP51-1), HIPA 6 (A2 dan B8094), HIPA Jatim 3 (A6 dan PK88) dan HIPA 14 SBU (A7 dan BH33d-Mr-57-1-2-2). Anak petak ialah tiga taraf frekuensi penyemprotan GA3 terdiri atas : kontrol (W0), dua kali aplikasi (W1) dan tiga kali aplikasi (W2). Dosis GA3 yang digunakan adalah 200 ppm yang merupakan konsentrasi terbaik dari hasil penelitian terdahulu. Hasil penelitian menunjukkan bahwa aplikasi GA3 mampu meningkatkan tinggi tanaman, eksersi malai, eksersi stigma dan durasi bunga membuka dibandingkan kontrol. Hasil yang terbaik dicapai pada perlakuan GA3 dengan dua kali aplikasi (W1). Hasil benih  pada perlakuan W1 mencapai 1429 kg/ha, lebih tinggi dibandingkan dengan aplikasi tiga kali GA3 (1215 kg/ha) dan kontrol (703 kg/ha).Kata kunci : GA3, padi hibrida, hasil benih