Ramadhani Eka Putra
Sekolah Ilmu dan Teknologi Hayati Institut Teknologi Bandung

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EFIKASI PROPOLIS LEBAH TRIGONA SP. SEBAGAI BAHAN EDIBLE COATING UNTUK PERLINDUNGAN PASCA PANEN BUAH PISANG AMBON LUMUT (MUSA ACUMINATA L.) Fathurrahman, Imam; Putra, Ramadhani Eka
JURNAL AGROTEKNOLOGI Vol 10 No 01 (2016)
Publisher : Faculty of Agricultural Technology, University of Jember

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Abstract

This study aimed to determine the efficiency of the use of propolis as a biocoating for post-harvest bananas in order to extend shelf life. This study used local banana that typical in Indonesia, Moss banana (Musa acuminata) and the propolis bee Trigona laeviceps, which is also produced by local agriculture, with a concentration of application 5%, 10%, 15%, coating using 70% ethanol as a positive control, and bananas without coating as a negative control. Observation parameters that measured are weight loss, pulp to peel ratio, size, color, fruit temperature, cell death, fruit firmness, total soluble solid (TSS), sucrose content, vitamin C, and potassium content on days 0, 3, 6, 9, 12, 15, 18 and 21 after coating. The results showed that coating using 5% propolis significantly extend the shelf life compared to the control by slowing the increase in fruit maturity index (color), weight loss, size loss, pulp to peel ratio, fruit temperature, pulp softening, and increased levels of vitamin C. Treatment process itself does not have a significant effect on changes in the value of TSS and sucrose during the storage period.Keywords: propolis, moss banana, post-harvest protection.
Pola kunjungan serangga liar dan efek penambahan koloni Trigona (Tetragonula) laeviceps Smith pada penyerbukan kabocha (Cucurbita maxima) Putra, Ramadhani Eka; Subagio, Julio; Kinasih, Ida; Permana, Agus Dana; Rosmiati, Mia
Jurnal Entomologi Indonesia Vol 14, No 2 (2017): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.14.2.69

Abstract

Serangga penyerbuk liar maupun terdomestikasi memainkan peran penting dalam proses penyerbukan pada berbagai tanaman bernilai ekonomi terutama tanaman monoceious seperti dari kelompok Cucurbitaceae. Tujuan dari penelitian ini adalah mendapatkan informasi mengenai peran dari serangga penyerbuk liar dan aplikasi koloni Trigona (Tetragonula) laeviceps Smith terhadap produksi dan kualitas buah kabocha (Cucurbita maxima) sebagai model tanaman pada lahan pertanian tradisional. Pengamatan dilakukan pada pukul 07:00–15:00 selama periode pebungaan dalam kurun waktu tiga minggu. Pengamatan dilakukan pada tiga plot perlakuan: open pollination + T. laeviceps (OPT), open pollination (OP), dan hand pollination (HP). Efek dari polinator ditinjau melalui tiga parameter, yaitu pola kunjungan, plant reproductive success, dan kualitas buah. Pola kunjungan diukur berdasarkan frekuensi dan lama kunjungan serangga, plant reproductive success diukur dari fruit set dan jumlah biji, sedangkan kualitas buah diukur berdasarkan diameter dan berat buah serta produksi buah/tanaman. Hasil penelitian menunjukkan mayoritas polinator liar aktif pada pagi hari, sedangkan T. laeviceps lebih aktif mulai siang yang diduga disebabkan oleh pengaruh variasi toleransi terhadap suhu pada tiap spesies. Terdapat perbedaan signifikan pada fruit set antara perlakuan OPT (80%), OP (65%), dan HP (38.3%) (F2,35 = 19.907, P = 0.000). Jumlah biji dan kualitas buah yang dihasilkan pada perlakuan OPT dan OP lebih tinggi dibandingkan dengan perlakuan HP, sedangkan jumlah buah pada OPT jauh lebih tinggi dibandingkan dengan lainnya. Rendahnya kesuksesan penyerbukan pada plot HP menunjukkan ketepatan waktu penting bagi penyerbukan kabocha. Berdasarkan hasil ini maka penyerbukan oleh lebah pada tanaman kabocha sulit digantikan oleh manusia dan variasi kunjungan yang dimiliki oleh T. laeviceps berdampak positif pada produksi buah kabocha.  
Toksisitas beberapa jenis fungisida komersial pada serangga penyerbuk, Trigona (Tetragonula) laeviceps Smith Kinasih, Ida; Nugraha, Rusdy Syachrul; Putra, Ramadhani Eka; Permana, Agus Dana; Rosmiati, Mia
Jurnal Entomologi Indonesia Vol 14, No 1 (2017): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.14.1.29

Abstract

Salah satu pestisida yang umum digunakan pada sistem perkebunan adalah fungisida. Walaupun spesifik didesain untuk mengatasi serangan cendawan, beberapa penelitian menunjukkan bahwa fungisida memiliki efek neurotoksik terhadap hewan uji. Hal ini memberikan potensi merugikan bagi hewan-hewan non target yang menguntungkan pada sistem pertanian, seperti serangga penyerbuk. Penelitian ini dilakukan untuk menentukan toksisitas dari fungisida yang beredar di pasaran pada salah satu agen penyerbuk Trigona (Tetrgonula) laeviceps Smith, dengan parameter toksisitas berupa nilai LD50 dan Hazard Quotient (HQ). Fungisida yang diujikan adalah dari golongan triazole (triadimefon dan difenoconazole), golongan propamokarb (propamokarb HCl), dan golongan ditiokarbamat (mankozeb dan propineb). Lebah pencari makanan T. laeviceps dikoleksi dan dianestesi dengan suhu -10 °C selama 1 menit. Sebanyak 1 μl larutan triadimefon, difenoconazole, propamokarb HCl, mankozeb, dan propineb diaplikasikan secara topikal dengan microsyringe di bagian dorsal lebah. Setiap kelompok perlakuan terdiri atas 10 individu lebah yang diulangi sebanyak tiga kali. Pengamatan mortalitas lebah dilakukan 48 jam setelah aplikasi. Hasil penelitian menunjukkan bahwa nilai LD50 (48 jam) dan HQ untuk fungisida uji adalah triadimefon 13,43 ±1,78 μg/lebah, HQ = 18,62; difenoconazole 9,25 ± 1,21 μg/lebah, HQ = 27,03; propamokarb HCl 270,45 ±19,48 μg/lebah, HQ = 14,34; mankozeb 3,75±0,47 μg/lebah, HQ = 512; dan propineb 9,71 ±2,20 μg/lebah, HQ = 144,18. Dari kelima fungisida, terdapat dua fungisida yang memiliki toksisitas moderat dan memiliki potensi menyebabkan mortalitas bagi lebah pencari makanan dan merugikan bagi koloni lebah saat aplikasi di lapangan, yaitu mankozeb dan propineb.
GROWTH PERFORMANCE OF BLACK SOLDIER FLY LARVAE (HERMETIA ILLUCENS) FED ON SOME PLANT BASED ORGANIC WASTES Kinasih, Ida; Putra, Ramadhani Eka; Permana, Agus Dana; Gusmara, Finsa Firlana; Nurhadi, Muhammad Yusuf; Anitasari, Ramadini Aini
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.936 KB) | DOI: 10.4308/hjb.25.2.79

Abstract

Insect larvae, such as black soldier fly larvae (BSFL: Hermetia illucens) are currently being considered as an alternative source of protein for poultry and aquaculture industry while in the same time apply as agent to manage organic wastes. In order to achieve both roles it is necessary to develop an appropriate farming methodology and understand the impact of different feeding source for the insect production. The study explored the effect of three local plant based organic wastes as feedstocks to growth of the black soldier fly larvae. Separate feeding trials on horse manure (HM), vegetable wastes (VW), and tofu dreg (TD) was done and their effect on BSF larvae growth rate, larvae weight and total prepupal yield in grams content analysed. The efficiency of the BSF larvae to consume and ability to reduce the waste load of the different substrates was also evaluated. The result showed all materials suitable as feeding material for black soldier fly. Difference on physical properties and chemical content affected the development time, harvested biomass, efficiency of digestivity, and efficiency of biomass production which is unique for each type of waste. This study provide base line information for future development of plant based organic waste management.
ANALISIS FAKTOR-FAKTOR PENYEBAB TERJADINYA LEDAKAN KASUS MALARIA DI KECAMATAN CINEAM, KABUPATEN TASIKMALAYA PADA TAHUN 1998 Putra, Ramadhani Eka; B. Wicaksono, Satrio Aribowo
JURNAL ISTEK Vol 8, No 2 (2014): ISTEK
Publisher : JURNAL ISTEK

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Abstract

Pada tahun 1998, kecamatan Cineam di kabupaten Tasikmalaya, Jawa Barat, mengalami Kejadian Luar Biasa (KLB) malaria dengan jumlah kasus mencapai 800 kasus sehingga Pusat Kesehatan Masyarakat (PUSKESMAS) kecamatan Cineam berada pada stratifikasi High Case Incidence (HCI). Kondisi berbeda terjadi pada tahun 2009 dan 2010 dimana jumlah kasus yang terjadi adalah 15 dan 6 kasus, secara berurutan. Penelitian ini bertujuan untuk melakukan analisis terhadap faktor-faktor yang menyebabkan KLB malaria tahun 1998. Metoda pengambilan data dengan (1) pencuplikan nyamuk Anopheles menggunakan CO2 Trap di 5 dari 10 desa pada kecamatan Cineam untuk mengetahui populasi nisbi dari nyamuk vektor malaria dan (2) wawancara dengan petugas pemberantasan penyakit menular (P2M) yang bertugas pada PUSKESMAS kecamatan Cineam saat KLB pada tahun 1998. Hasil yang diperoleh pada penelitian ini, dari 320 individu nyamuk Anopheles yang ditangkap, terdapat empat jenis nyamuk Anopheles dengan tingkat dominansi, Anopheles vagus (33,13%), Anopheles aconitus (28,75%), Anopheles barbirostris (23,75%), dan Anopheles kochi (14,38%) dimana hanya An. aconitus dan An. barbirostris yang merupakan vektor malaria. Keberadaan nyamuk Anopheles ini didukung oleh faktor klimat serta keadaan lingkungan setempat yang menyediakan feeding place, resting place, dan breeding site bagi nyamuk Anopheles. Walaupun demikian, keberadaan populasi nyamuk Anopheles bukan merupakan faktor utama penyebab KLB malaria, melainkan rendahnya pemahaman masyarakat pada penyakit ini. Hal ini ditunjukkan oleh rendahnya tingkat prevalensi malaria yang sangat rendah (Low Case Incidence) yaitu pada tahun 2009 dan 2010 (sebesar 0,44‰ dan 0,18‰ secara berurutan) setelah dilakukan penanggulangan penyebaran malaria dalam bentuk penyuluhan pada warga sejak KLB pada tahun 1998.
Effect of Water Temperature to Survival and Development of Larvae of Two Local Aedes Aegypti Strains Putra, Ramadhani Eka; Trinuroni, Gunadi
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.3843

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  Global warming has been reported in last decades. Chang-es in average Earth’s temperature may affect the physiology of many insect species, especially the ones which act as a human disease vec-tor, like Aedes aegypti. In this study, the effect of increasing water temperature on physiological components related to development period, sex ratio, and body size of two distinctively strains, VCRU (Vector Control Research Unit) and Pangandaran. Thirty larvae of each strain kept inside distilled water with the temperature of 25°C, 27°C, 30°C, 33°C, 35°C, 40°C which replicated three times. Observa-tions were conducted until all larvae metamorphed into adults or all larvae dead. Development rate and time were analyzed by frequency dependent mean. The result showed that the optimum temperature for larvae development of VCRU strain was 27-30°C with a survival rate of 84% while it was 30°C for Pangandaran strain, with the surviv-al rate of 83%. Larvae of both strains showed 100% mortality rate when kept inside a container with water temperature exceed 33°C. Both strains showed the highest and the lowest male:female ratio at similar water temperature which were 33°C and 30°C, respec-tivelly. Highest and lowest ratio of VCRU was 1.25 and 0.6, respec-tively, while it was 1.4 and 0.6 for Pangandaran. In general, larvae lived in increasing water temperature showed reducing wing width. 
The Potency of Trigona’s Propolis Extract as Reactive Oxygen Species Inhibitor in Diabetic Mice Ridwan, Ahmad; Sari, Ayu Nirmala; Putra, Ramadhani Eka
Journal of Mathematical and Fundamental Sciences Vol 47, No 3 (2015)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.633 KB) | DOI: 10.5614/j.math.fund.sci.2015.47.3.4

Abstract

Hyperglycemia has been proven to increase oxidative stress due to the production of reactive oxygen species (ROS) that exceed the capabilities of the natural antioxidant defenses, causing a deficiency in insulin receptors and insulin resistance. In this study, the effect of propolis on ROS was observed. Fifty five (55) male mice (Mus musculus SW.) were divided into 5 groups, i.e. KN (normal control), KDM (diabetes control), and P1, P2, P3. Propolis solution 50, 100 and 175 mg/kg bw was given to groups P1, P2 and P3 respectively, while distilled water was given to groups KN and KDM by oral gavage for 21 days. Density of ROS was measured every 7 days, while measurement of plasma insulin was carried out every 3 days. The results show that the density of ROS in the groups treated with propolis was lower than in the KDM group. However, the plasma insulin levels in the propolis groups were higher than in the KDM group. It was concluded that propolis can decrease ROS density and causes an increase in plasma insulin levels.
Infeksi Subletal Bacillus thuringiensis pada Helicoverpa armigera: Mungkinkah Menyebabkan Resurgensi? Anggraeni, Tjandra; Putra, Ramadhani Eka; Ahmad, Intan
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 1 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9946.571 KB) | DOI: 10.22146/jpti.10016

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Research on the effect of B. thuringiensis sublethal infection to the chance of H. armigera to resurgence has been conducted. Third instar insects were used and the concentration applied were 0 ppm, 150 ppm, 300 ppm, 450 ppm and 600 ppm. The result showed that along with the increase of B. thuringiensis infection, the number and the viability of the eggs production declined. In addition, the weight difference between the 3rd and the 4th instar became higher however between the 4th and the 5th became lower, moreover, the weight of female imago decreased, the life longevity of male and female imago were shorter and longer, respectively, along with the increase of B. thuringiensis infection. The possibility for resurgence will be discussed.Key words: B. thuringiensis, H. armigera, sublethal, resurgence
Aplikasi campuran serbuk kayu pinus dan fipronil sebagai umpan rayap tanah Macrotermes gilvus (Hagen) (Isoptera: Termitidae) di Bandung Amran, Amran; Ahmad, Intan; Putra, Ramadhani Eka; Kuswanto, Eko
Jurnal Entomologi Indonesia Vol 12, No 2 (2015): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.12.2.73

Abstract

In this study, termite bait comprising pinewood of sawdust and a slow action insecticide, fipronil, was made and applied to control subterranean termite Macrotermes gilvus (Hagen) (Isoptera: Termitidae) in Bandung. Colony size was measured using capture-mark-release-recapture prior to the bait application, along with the measurement of wood consumption at each station.  Colony foraging populations at all stations were estimated to be 77,951 termites, with mean wood consumption rates ranging from 0.02 to 6.16 g/station/day. Observation at 18 stations installed with bait consisted of 40 ppm fipronil mixed with pinewood sawdust showed that number of foraging activity termites was effectively reduced in 40 days. It is concluded that fipronil-treated pinewood sawdust bait is effective in controlling the population of foraging M. gilvus workers in 40 days.
Pertumbuhan dan perkembangan larva Musca domestica Linnaeus (Diptera: Muscidae) dalam beberapa jenis kotoran ternak Putra, Ramadhani Eka; Rosyad, Abdul; Kinasih, Ida
Jurnal Entomologi Indonesia Vol 10, No 1 (2013): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.10.1.31

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House fly (Musca domestica) is an important urban insect that can transmit various infectious diseases. This insect usually utilized organic wastes as nutrition source for their larvae. One of the main sources of organic wastes is livestock manure produced by animal farming located near human dwelling area. Thus, appropriate house fly population control program at animal farm is needed,based on information on the house fly’s life history. The research is focused on the development of house fly larvae reared with different livestock manures, such as cow, chicken, and horse. As comparison, rice bran were used as control. Results showed that larvae reared with horse manure has the shortest development time (5 days), with lowest larval survival rate (30%), pupal weight (6.8 ± 0.141 g), and weight of female imago (4.9 ± 0.14 g). This finding indicates the lowest nutrition value of horse manure for house flies larvae. Further research is needed to find the effect of manure to variables that directly influence population growth, such as fecundity of female flies and egg survivorship. These additional information on life history will help the design of appropriate house fly population management program for animal farm.