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SELEKSI ISOLAT BAKTERI AMILOLITIK DARI RHIZOSFER CANNA EDULIS, KERR. UNTUK PRODUKSI POLI HIDROKSI ALKANOAT DARI LIMBAH CAIR TAPIOKA Nurhayati, Nurhayati; Radjasa, Ocky Karna; Prijambada, Irfan Dwidya
Jurnal Rekayasa Proses Vol 11, No 2 (2017)
Publisher : Departemen Teknik Kimia Fakultas Teknik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.8 KB) | DOI: 10.22146/jrekpros.33194

Abstract

Petrochemical-based plastic waste accumulated in landfills have been posing serious threat to the environment as this kind of plastics are non-biodegradable. Replacing petrochemical-based plastics with biodegradable plastics constitutes a challenging solution both in terms of mechanical design of the process and most importantly the availability of powerful local microorganism for the process. Therefore, the current study was searching for appropriate local microorganisms for poly hydroxyl alkanoate (PHA) production from starch waste, which was considered as one of cheap carbon sources. Waste water of cassava industry is a good resource of such starch waste water. The microbes were isolated from Canna edulis, Kerr. rhizosphere from Cangkringan.  The expected isolates were the bacteria enable the coupling of carbon catabolic pathways with PHA anabolic pathways. It was found that ten isolates were able to use waste water of cassava flour industry as carbon source. The PHA quantitative analysis by spectrophotometer showed that the isolate of Bacillus sp. C8 produced the highest PHA of 2,095 g/L. Further FTIR analysis showed specific bands near 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 wavelengths which revealed the presence of CH3, ester carbonyl group (C=O), C-H and terminal OH group of PHA. ABSTRAKAkumulasi sampah plastik berbasis petrokimia di tempat pembuangan sampah mengganggu lingkungan karena plastik sifatnya tidak mudah didegradasi secara biologi dan sangat tahan di lingkungan. Penggantian plastik yang berasal dari bahan petrokimia dengan bahan plastik yang mudah terdegradasi secara biologi merupakan tantangan tersendiri, baik dari sisi perancangan proses maupun ketersediaan mikrobia lokal yang sesuai untuk proses tersebut. Oleh karena itu, penelitian ini dilakukan sebagai upaya untuk mendapatkan bakteri lokal penghasil PHA yang mampu mengkonsumsi substrat berupa pati. Substrat pati dipilih karena ketersediaan limbat industri tapioka sebagai bahan baku potensial dan murah untuk produksi PHA. Bakteri amilolitik untuk produksi PHA telah berhasil diisolasi dari rhizosfer Canna edulis, Kerr. di Cangkringan, Sleman, Yogyakarta, Indonesia. Bakteri yang diisolasi merupakan bakteri dengan kemampuan memproduksi PHA dan memiliki kemampuan melakukan rangkaian reaksi pada limbah cair industri tapioka dan rangkaian reaksi pembentukan PHA. Telah berhasil didapatkan 10 bakteri yang memiliki aktivitas amilolitik dan dapat menghasilkan PHA  menggunakan limbah cair industri tapioka. Analisis kuantitatif PHA menggunakan spektrofotometer menunjukkan bahwa isolat Bacillus sp. C8 menghasilkan PHA paling tinggi sebesar 2,095 mg/mL. Analisis hasil metabolism isolat C8 menggunakan FTIR memperlihatkan puncak spesifik 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 adalah verifikasi adanya CH3, C=O, C-H dan OH dari PHA.
PHYLOGENETIC RELATIONSHIP OF BAROPHILIC BACTERIA FROM NORTHWESTERN PACIFIC OCEAN Radjasa, Ocky Karna
JOURNAL OF COASTAL DEVELOPMENT Vol 5, No 1 (2001): Volume 5, Number 1, Year 2001
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

In order to elucidate the role of hydrostatic pressure on the distribution of marine microorganisms, deep-sea water samples from north-western Pacific Ocean were collected and used as the source of barophilic bacteria. One isolate, JTW-863 was obtained from a depth of 6000 m, while two isolates, MTW-1 and MTW-13, were obtained from a depth of 10,500 m. Phylogenetic analysis based on PCR-amplified 16S rDNA revealed that JTW-863 belonged to Moritella, while MTW-1 was mostly related to Shewanella. On the other hand, MTW-13 was affiliated with the uncultured clone NB1-d from deep environment.
POTENSI RUMPUT LAUT SARGASSUM DUPLICATUM SEBAGAI SUMBER SENYAWA ANTIFOULING Santi, Ika Wulan; Radjasa, Ocky Karna; Widowati, Ita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v3i3.5999

Abstract

Biofouling di laut menyebabkan kerusakan pada lambung kapal dan merusak infrastruktur kelautan lainnya. Rumput lautgenus Sargassum banyak dilaporkan sebagai alternatif sumber senyawa antifouling alami yang ramah lingkungan. Penelitian ini menggunakan S. duplicatum dari Perairan Teluk Awur Jepara yang diekstrak dengan berbagai pelarut (n-heksana, etil asetat dan metanol) untuk mengetahui potensi ekstrak sebagai antifouling dan golongan senyawanya serta untuk mengidentifikasi jenis bakteri biofilm yang sensitif terhadap ekstrak. Bakteri biofilm diisolasi dari panel kayu dan fiberglass yang dibenamkan dilaut selama 2 minggu. Uji aktivitas antimicrofouling menggunakan metoda disc diffusion, uji fitokimia menggunakan metoda Harborne (1987) dan uji biokimia menggunakan metoda Cowan and Steels (1974) dan Bergey?s (2005). Aktivitas antimicrofouling paling baik ditunjukan oleh ekstrak etil asetat yang dapat menghambat 22 dari 34 bakteri biofilm dengan kisaran zona hambat 0,65 ? 3,73 mm. Sedangkan, bakteri yang paling sensitif terhadap ekstrak S. duplicatum adalah bakteri K.10.3.1.4 dan F.10.3.1.2. S. duplicatum mengandung senyawa golongan alkaloid, saponin, quinon, fenolik, steroid, dan flavonoid. Berdasarkan identifikasi bakteri secara biokimia, bakteri K.10.4.1.5 adalah genus Achromobacter, sedangkan kode isolat F.10.3.1.2 adalah genus Flavobacterium cytophaga. Kehadiran satu ataulebih senyawa fitokimia dalam ekstrak diduga bertanggung jawab atas aktivitas antifouling yang terjadi.
SKRINING ANTIBAKTERI EKSTRAK RUMPUT LAUT SARGASSUM PLAGYOPHYLLUM DARI PERAIRAN BANDENGAN JEPARA TERHADAP BAKTERI PATOGEN ENTEROBACTER, PSEUDOMONAS AERUGINOSA DAN STAPHYLOCOCUS AUREUS Yunianto, Hendi Perdian; Widowati, Ita; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v3i3.5987

Abstract

Sifat resistensi dan infeksi patogenitas bakteri terhadap manusia saat ini meningkat, maka perlu adanya obat alternatif baru. Senyawa bioaktif yang terkandung dalam rumput laut merupakan salah satu sumber antibakteri baru yang diperoleh dari alam. Tujuan penelitian ini adalah mengetahui aktivitas antibakteri ekstrak Sargassum plagyophyllum terhadap bakteri patogen Enterobacter, P. aeruginosa dan S. aureus serta mengetahui golongan senyawa dan toksisitas senyawa bioaktif ekstrak S. plagyophyllum. Penelitian ini dilakukan pada bulan September 2012 ? Mei 2013. Pengambilan sampel dilakukan secara purposive sampling method. Metode yang digunakan dalam penelitian ini adalah metode eksperimental laboratoris. Ekstraksi dilakukan secara bertingkat dengan menggunakan tiga pelarut yang berbeda kepolarannya. Uji aktivitas antibakteri dilakukan menggunakan metode difusi agar (Kirby-bauer) dengan 3 kali pengulangan. Analisis fitokimia dilakukan secara deskriptif kualitatif berdasarkan perubahan warna serta karakteristik fisika kimia suatu golongan. Uji toksisitas dilakukan menggunakan larva Artemia salina. Nilai toksisitas akut (LC50) ditentukan dengan menggunakan persamaan regresi linier. Hasil penelitian menunjukkan bahwa ekstrak Sargassum plagyophyllum pelarut etil asetat memiliki aktivitas antibakteri terbaik dengan zona hambat 4,87 ± 0,49 (bakteri Enterobacter); 6,06 ± 0,58 (bakteri S. aureus) dan 6,30 ± 0,62 (bakteri P. aeruginosa) serta memiliki aktivitas bakteriosidal. Hasil analisis fitokimia menunjukkan bahwa secara umum ekstrak Sargassum plagyophyllum mengandung senyawa alkaloid (pelarut heksana), steroid (ketiga pelarut), saponin (pelarut metanol). Hasil uji toksisitas menunjukkan ekstrak S. plagyophyllum pelarut etil asetat memiliki toksisitas yang toksik dengan nilai LC50-24 jam sebesar 291 ppm (toksik kategori kronik).
UJI FITOKIMIA DAN TOKSISITAS EKSTRAK KASAR GASTROPODA (TELESCOPIUM TELESCOPIUM) TERHADAP LARVA ARTEMIA SALINA Putri, Mukti K. Diana; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v1i2.2020

Abstract

Gastropod is one of the marine organisms that can be used as a source of natural bioactive compounds, one of which is Telescopium telescopium. T. telescopium until now it has not been yet exploited optimally, some people still use it as feed ingredients that contain high protein nutrition, however it must be supported by scientific information about the efficacy and side effects. This research aims at determining the secondary metabolite by crude extract T. telescopium, and the lethal toxicity value (LC50-24 hours). The research consisted of two steps, namely the phytochemical and toxicity test. The method used exploratif and experimental laboratory with a complete randomized design, which consited of five concentration treatments namely 25.12, 63.1, 158.5, 398.15, and 1000 ppm with three replications. Each treatment used 10 Artemia salina larvae, and observations were made during 24 hours of Artemia salina larvae mortality. LC50 was determined by probit analysis using the EPA Probit Analysis Program Version 1.5 Finney. The results of the phytochemical test showed that crude extract T. telescopium contain alkaloid compounds, steroids, flavonoids. The results BSLT (Brine Shrimp Lethality Test) of crude extract T. telescopium LC50-24 hours showed that the crude extract clorofom (229.562 ppm), crude extract ethyl acetate (244.906 ppm), and crude extract methanol (197.242 ppm). LC50-24 hours < 1000 ppm showed that the crude extract Telescopium telescopium potent antitumor compounds.
IDENTIFIKASI PIGMEN KAROTENOID PADA BAKTERI SIMBION RUMPUT LAUT KAPPAHYCUS ALVAREZII Sahara, Fera Nur Idawati; Radjasa, Ocky Karna; Supriyantini, Endang
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v2i3.3133

Abstract

Carotenoid is one of the most important pigments that have important roles for human health. Carotenoids are believed to improve the better immune responses, anticancer, antioxidant, provitamin A and are also used in the treatment of disease that are sensitive to light. Carotenoids are yellow pigments, orange to red pigments and usually found in vegetables and fruits, and are also found in animals, humans, fungi and bacteria. The aims of study were to analyze carotenoid pigments from bacterial symbionts from seaweed Kappaphycus alvarezii and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Bacterial identification was performed based on biochemical tests. These results showed that from 12 bacterial symbionts isolated, there were 2 isolates that positively synthesize carotenoids pigments, ECJ K and ECJ OR bacteria. ECJ K bacterium contained of Violaxanthin and ?-carotene pigments. While ECJ OR bacterium contained of Diadinoxanthin, Neoxanthin, ?-carotene, ?-carotene and ?-carotene pigments. Bacterial identification showed that ECJ K bacterium was Brevibacterium maris and ECJ OR bacterium was Micrococcus varians. This result showed that bacterial symbionts of seaweed Kappaphycus alvarezii contained various pigments including in carotenoid pigments.
POTENSI PIGMEN KAROTENOID BAKTERIUM ENDOFIT LAMUN THALASSIA HEMPRICHII SEBAGAI SUMBER SENYAWA ALAMI PENANGKAL RADIKAL BEBAS DPPH (1,1-DIFENIL-2-PIKRILHIDRAZIL) Permata, Rr. Citra; Riniatsih, Ita; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v3i3.6001

Abstract

Karotenoid merupakan salah satu jenis pigmen yang penting bagi kesehatan manusia yang berfungsi sebagai antioksidan. Karotenoid merupakan pigmen kuning, orange sampai merah yang biasanya ditemukan dalam sayuran, buah-buahan serta bakteri dan fungi. Penelitian ini bertujuan mengetahui aktivitas antioksidan dari pigmen karotenoid bakteri endofit lamun Thalassia hemprichii serta mengidentifikasi bakteri tersebut secara molekuler. Analisis pigmen dilakukan dengan menggunakan spektrofotometer UV 1601, uji antioksidan dengan metode DPPH dan identifikasi bakteri dilakukan dengan PCR 16S rDNA. Penelitian ini dilaksanakan pada bulan Oktober 2013 ? April 2014. Sampel lamun didapatkan dari perairan Teluk Awur Jepara. Hasil penelitian menunjukkan bahwa bakterium Th1 mempunyai pigmen kuning dengan tiga puncak panjang gelombang yaitu 460 nm, 439.5 nm, 417 nm sesuai dengan serapan panjang gelombang pigmen karotenoid yaitu 300-600 nm. Identifikasi bakteri secara molekuler 16S rDNA menunjukkan bahwa isolat Th1 mempunyai homologi 97% dengan Erythrobacter vulgaris. Aktivitas penghambatan pigmen bakteri Th1 yaitu 18,97%.. Berdasarkan hasil penelitian dapat disimpulkan bahwa pigmen karotenoid bakterium Th1 mempunyai potensi sebagai senyawa alami penangkal radikal bebas DPPH.
DEEP- SEA BACTERIA AND THEIR BIOTECHNOLOGICAL POTENTIALS radjasa, Ocky Karna
JOURNAL OF COASTAL DEVELOPMENT Vol 7, No 3 (2004): Volume 7, Number 3, Year 2004
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Deep-sea environment characterized by its low temperature, high hydrostatic pressure, limited nutrient contents, and constant darkness has been a hostile environment for most marine microorganisms. Therefore it is a home for low temperature and high pressure-loving bacteria believed to functionally over shallow water intruders. Deep-sea has been regarded as rainforests in terms of their microbial diversity. Thus, the deep-sea should commend itself to microbiologists and biotechnologists alike as source of novel microorganisms and exploitable properties. Near Indonesia several deep-sea trenches exist with diverse environmental conditions. However, up to now there has been no effort to study the microbial communities of those deep-sea environments by Indonesian scientists. This is mainly because of the lack of knowledge in the field of deep-sea microbiology in Indonesia.
UJI FITOKIMIA DAN AKTIVITAS ANTIJAMUR EKSTRAK TERIPANG KELING (HOLOTURIA ATRA) DARI PANTAI BANDENGAN JEPARA TERHADAP JAMUR CANDIDA ALBICANS Septiadi, Tedi; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v2i2.2355

Abstract

Holothuria atra is one of the marine lifes that lives at the bottom of the substrate, and able to adapt to its environment. Several previous studies showed that extracts of H. atra from different waters have activity as an antifungal. Fungus Candida albicans is one of human pathogens that attack on the mucosa of the mouth, skin and vagina. The purpose of this study were to identify the compounds contained in the extracts of H.atra and examine the effect of extracts concentration against C. albicans. The process of extraction was done by maceration with solvent n-hexane, ethyl acetate and methanol. Testing of secondary metabolites was carried out using phytochemical screening methods while testing antifungal activity was using agar diffusion test. The results showed that the extracts of H.atra contained saponins, alkaloids, steroids and triterpenoids. Antifungal activity assays showed that the n-hexane extract did not show any inhibition zone, while the ethyl acetate and methanol extracts showed inhibition zone at a concentration of 1 mg / disk with a large zone of inhibition of 8.27 ± 0.06 and 8.07 ± 0 , 12 mm, respectively based on these results it can be concluded that the extract of ethyl acetate solvent H.atra has strong potential as antifungal.
RAPID GROUPING OF MARINE PSYCHROTROPIC BACTERIA USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF PCR-AMPLIFIED 16S RDNA Radjasa, Ocky Karna; Urakawa, Hidetoshi; Kita-Tsukamoto, Kumiko; Ohwada, Kouichi
JOURNAL OF COASTAL DEVELOPMENT Vol 3, No 1 (1999): Volume 3 Number 1 Year 1999
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Grouping of 78 psychrotrophic bacteria isolated from surface and deep-sea waters of the north-western Pacific Ocean was carried out by RFLP analysis on the basis of PCR-amplified 16S rDNA. Six RFLP patterns from surface water and eight RFLP patterns from deep-sea water were obtained by HhaI digestion. F pattern was only found in the surface water, while g, h, and I patterns exclusively exited in the deep-sea water. It was clearly shown that RFLP patterns of HhaI digestion were clear and distinguishable. This method is very simple and promises a rapid tool to group numerous isolated from natural environments.
Co-Authors A B Susanto A. Sabdono Adi Santoso Afriyanto, Rachmat Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Trianto Awang Surya Wiguna, Awang Surya Bachtiarini, Tiara Ulfa Bahry, Muhammad Syaifudien Boedi Hendrarto Damar Bayu Murti, Damar Bayu Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Edi Suhartono Elena Zocchi Endang Supriyantini Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu, Ferdy Semuel Ferry F. Karwur Ferry Fredy Karwur Gede Iwan Setiabudi, Gede Iwan Handung Nuryadi HANS- PETER GROSSART Hefni Effendi Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Iqna Kamila Abfa IRFAN DWIDYA PRIJAMBADA Ita Riniatsih Ita Widowati JOEDORO SOEDARSONO Johannes Hutabarat Khoeri, Miftahuddin M Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Kustiariyah Tarman Leenawaty Limantara Lia Kusmita Marhaeni, Bintang Meinhard Simon Meutia Samira Ismet Miftahuddin Majid Khoeri Misbakul Munir Muchlisin, Sakti Imam MUJIZAT KAWAROE Mukti K. Diana Putri Nestin E.W. Ningrum Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo, . Pratiwi Sudharmono, Pratiwi Radjasa, Septhy Kusuma Rahmawati, Meris Richardus F Kaswadji Riyanti Riyanti Rr. Citra Permata Rudhi Pribadi S arjito S. Sulistiyani, S. Sabdaningsih, Aninditia Sahala Hutabarat Sarjito Sarjito Sibero, Mada Triandala Sibero, Mada Triandala Slamet B Prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Stöhr, Rudiger Subagiyo Subagiyo Suharsono . Supriharyono ,, Supriharyono Suyono ,, Suyono Tedi Septiadi Tonny Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Uswatun Khasanah Utami, Ni Komang Tri Vivi Endar Herawati Widyaningsih, Sekar Widyastuti, Yustin Wiratno Wiratno Wittriansyah, Khoeruddin Wiwik Astuti Yatnita Parama Cita Yusidharta, Isai