RIKA RAFFIUDIN
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University,

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Morphological Variation of the Asian Honeybee Apis cerana (F.) (Hymenoptera: Apidae) in West Java. The diversity of Asian honeybee Apis cerana (F.) (Hymenoptera: Apidae) occupying low and high altitude habitats in West Java was studied using morphological analysis, based on the length of proboscis, hindleg, front wing, tergites, and sternites. All morphological characters measured were signifcantly longer in high altitude bees compareded to those of low altitude bees (p < 0.05), except probos Raffiudin, Rika; Sosmarsono, Soemartono; Ratna, endang S.; Solihin, Dedy D.
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 1 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Abstract

Morphological Variation of the Asian Honeybee Apis cerana (F.) (Hymenoptera: Apidae) in West Java. The diversity of Asian honeybee Apis cerana (F.) (Hymenoptera: Apidae) occupying low and high altitude habitats in West Java was studied using morphological analysis, based on the length of proboscis, hindleg, front wing, tergites, and sternites. All morphological characters measured were signifcantly longer in high altitude bees compareded to those of low altitude bees (p < 0.05), except proboscis. Dendogram analysis showed that bees from those two altitudes were separated, whereas the result of principal component analysis indicated that both belong to the same group (P < 0.05).
Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne Djakaria, Shafira Ananda; Batubara, Irmanida; Raffiudin, Rika
Jurnal Kimia Sains dan Aplikasi Vol 23, No 8 (2020): Volume 23 Issue 8 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.254 KB) | DOI: 10.14710/jksa.23.8.267-275

Abstract

Honey has natural bacteriostatic and bactericide activities; thus, it could be active compounds as an antibacterial against Propionibacterium acnes, the main bacteria in acne forming state. Our study aims to determine phenolic and flavonoid contents (qualitative and quantitative), antioxidants, along with antibacterial activity against P. acnes of selected Indonesian honey. Honey used in this study, including honeybees and stingless bees, were collected from Sumbawa, Riau, Belitung, Sukabumi, Banyuwangi, Bogor, and South Sulawesi. In the method, the quantitative content of phenols and flavonoid were measured using a spectrophotometer. Further, DPPH (2, 2’-diphenylpicryl hydrazyl) and ABTS (2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) assays were used to measure the antioxidant activities, and subsequently microdilution method was applied to determine antibacterial activity. The results showed that Indonesian honey contains phenolics and flavonoids in the range of 49.5-67.3 μg Gallic Acid Equivalent (GAE)/g and 766.5-1509.5 μg Quercetin Equivalent (QE)/g, respectively. All honey samples also show antioxidant and antibacterial activities. Of note, Apis cerana honey from Banyuwangi has the highest antioxidant and antibacterial activity compared to others. It has the Inhibition Concentration (IC50), Trolox Equivalent Antioxidant Capacity, Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) values of 59.85 mg/L, 122 μg Trolox/g, 125 mg/mL, and 500 mg/mL respectively. Our study reveals that Indonesian honey can inhibit and kill P. acnes as well. Nonetheless, the phenolic content of honey is not always linear with antioxidant and antibacterial activities.
KARAKTERISASI MORFOLOGI TIGA GENUS SERANGGA PENGGEREK (LEPIDOPTERA: PYRALOIDEA) Pratami, Gina Dania; Raffiudin, Rika; Samudra, I Made
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.32 KB) | DOI: 10.23960/j.hptt.216155-164

Abstract

Morphological characterization of three genere of insect borer (Lepidoptera: Pyraloidea). The objective of the research was to characterize the morphological differences of insect borers between Genus Etiella (Pyralidae: Phycitinae), Scirpophaga (Crambidae: Schoenobiinae), and Ostrinia (Crambidae: Pyraustinae). Observed characters were based on external morphology and genitalia. The result showed that Crambidae has praecinctorium in the tympanic organs, while lack of in Pyralidae. Phycitinae had chaetosema, proboscis, cubital pecten and the elongated forewing. Pyraustinae was lack of chaetosema and their forewings are wide towards termen. Whereas, Schoenobiinae had chaetosema with elongated forewing. Etiella had scales on antemedial area and their veins M2-M3 are fused. Forewing of Ostrinia had 11 veins and the corpus bursae shape was round irregular. Forewing of Scirpophaga had 12 veins, anal hair tuft, coremata, and the corpus bursae is round. The main characteristics used in identification at family and subfamily level were the praecinctorium, chaetosema, the shape of the forewings, proboscis, and cubital pecten. Whereas at genus level; anal hair tuft, coremata, and shape of the corpus bursae formed the basis of characterization. The morphological characterization was used to make the key identification of insect borers in Indonesia.
Kekerabatan rayap tanah Macrotermes gilvus Hagen (Blattodea: Termitidae) dari dua habitat di Bogor Mubin, Nadzirum; Hararap, Idham Sakti; Giyanto, Giyanto; Raffiudin, Rika
Jurnal Entomologi Indonesia Vol 12, No 3 (2015): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.864 KB) | DOI: 10.5994/jei.12.3.115

Abstract

Subterranean termites Macrotermes gilvus (Hagen) (Blattodea: Termitidae) belongs to the subfamily Macrotermitinae they are distributed widely in Southeast Asia such as Indonesia, Malaysia, Singapore, Myanmar, Philippine, Vietnam, and Thailand. Many studies on these termites have been conducted, but the study of relatedness termites from different locations are barely done. The objective of this study was analyze the relatedness between subterranean termites M. gilvus in IPB Dramaga Campus and Yanlappa Nature Reserve, Jasinga-Bogor. The termite relatedness was analyzed with agonistic behavioral approach and molecularly, technique using mitochondrial COI gene. Termites from both locations showed identical molecular relatedness, however behavioral analysis show that they belong from different parental lineages. Agonistic assessment showed that individuals from different habitat showed aggressive behaviour, whereas those that originated from the same nest do not show any aggression. Molecular detection however have failed to show.
Diversity and Abundance of Carabidae and Staphylinidae (Insecta: Coleoptera) in Four Montane Habitat Types on Mt. Bawakaraeng, South Sulawesi Qodri, Agmal; Raffiudin, Rika; Noerdjito, Woro Anggraitoningsih
HAYATI Journal of Biosciences Vol. 23 No. 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (869.581 KB) | DOI: 10.4308/hjb.23.1.22

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Carabidae and Staphylinidae are the two beetle families frequently found to be most abundant and diverse in forest ecosystem. Their roles especially as generalist predators are important in forest ecosystem. No studies reported their diversity and abundance in forest ecosystem on Mt. Bawakaraeng, specifically in montane habitat yet. This study was aimed to analyze diversity and abundance of Carabidae and Staphylinidae in four montane habitat types, i.e. agricultural area, pine forest, eucalypts and natural forest (1,835 m asl), and natural forest (2,165 m asl). They were collected using pitfall traps. A total of 42 carabid beetles belonging to nine species and 260 staphylinid beetles belonging to 37 species were collected. Diversity and abundance of Staphylinidae were higher than Carabidae, this is predicted because of higher mobility in Staphylinidae compared to Carabidae. In Carabidae, the highest species richness was recorded in agricultural area, whereas the highest species richness of Staphylinidae occurred in natural forest (2165 m asl). In Staphylinidae, the two largest subfamilies are Aleocharinae and Staphylininae. Aephinidius adelioides occupied the highest abundance of Carabidae and found in agricultural area. The differences in each montane habitat type are presumed to cause variation in species richness of soil beetles, especially for Carabidae.
Characterization and Identification of Cellulolytic Bacteria from gut of Worker Macrotermes gilvus Ferbiyanto, Andri; Rusmana, Iman; Raffiudin, Rika
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (760.677 KB) | DOI: 10.4308/hjb.22.4.197

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As a social insect, termite colony consists of three castes, i.e. reproductive, soldier, and worker castes. In their role of cellulose digestion, the worker termites use two sources of cellulolytic enzyme that include cellulases produced by the termite and the gut symbions. Macrotermes gilvus classified in mound builder termite, mostly depend on cellulolytic bacteria for cellulose digestion. This study aims to characterize cellulolytic bacteria of termite gut symbionts of worker M. gilvus and to identify the cellulolytic bacteria based on sequences of 16S ribosomal RNA (rRNA) gene. Cellulolytic bacteria of termite gut were isolated and cultured in CMC (Carboxymethyl cellulose) media. The biochemical characters of bacterial isolates were assayed using Microbact 12A and 12B. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The bacterial isolate that has the highest cellulolytic index was analyzed for its 16S rRNA gene sequences. Four isolates of cellulolytic bacteria were successfully isolated from gut of M. gilvus with aerobic and anaerobic conditions. The highest formation of cellulolytic index (2.5) was revealed by RA2. BLAST-N (Basic Local Alignment Search Tool for Nucleotides) result of 16S rRNA gene sequences of RU4 and RA2 isolates showed that the isolate has similarity with Bacillus megaterium and Paracoccus yeei, respectively. This result indicated that RA2 isolate was P. yeei, a cellulolytic bacterium of a termite gut of M. gilvus.
Three Species of Ectoparasite Mites (Acari: Pterygosomatidae) Infested Geckos in Indonesia PRAWASTI, TARUNI SRI; FARAJALLAH, ACHMAD; RAFFIUDIN, RIKA
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.433 KB) | DOI: 10.4308/hjb.20.2.80

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Limited data is hitherto available on the diversity and dispersal of parasitic mites of geckos in Indonesia. Here, we collected three species of geckos, namely Cosymbotus platyurus, Hemidactylus frenatus, and H. garnotii throughout Indonesia to study the distribution and diversity of its parasitic mites. We conducted detail morphological analysis of the mites using whole mount polyvinyl lactophenol and scanning electron microscope preparation. Three species of ectoparasite mites from genus Geckobia were identified in a total of 221 individuals out of 448 geckos collected from 25 sites in Indonesia. Two species were G. glebosum and G. bataviensis, and the other one was designated as Geckobia sp 1. Based on our result, the three mites species were spread randomly and live sympatrically. The G. bataviensis mite showed the widest distribution, because it was found in almost all gecko collection sites, hence the most cosmopolitan mites. We also found that C. platyurus gecko had the lowest mite prevalence which might due to the fact that it has the least number of skin folds, an important site for mite protection. This result implies that further research on the relationship of anatomy of gecko skin with chelicera and claw structure of mites is necessary in the future.
Morphological Characters and Histology of Pheretima darnleiensis DARMAWAN, ANDY; RAFFIUDIN, RIKA; WIDARTO, TRI HERU
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.12 KB) | DOI: 10.4308/hjb.19.1.44

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Pheretima darnleiensis is a native earthworm of Southeast Asia, India, and Japan. Although it is commonly found in Indonesia, the earthworm has never been studied well. This study was aimed to examine the morphological characters and structure of its several organs for an identification purpose, which is important for the earthworm culture. Earthworms were collected in a plot of 55-150 x 55-150 cm width and 20 cm depth at Bogor Agricultural University in Darmaga and Baranangsiang Campuses by hand sorting method. Examinations were carried out on its external as well as internal characters. The histology of the organs was studied using paraffin method. The observed characters on P. darnleiensis were the presence of prostate gland, one pair of male pores on segment XVIII, a cylindrical body with perichaetine setae, caeca on segment XXVII, copulatory pouches without diverticula and stalked glands, bithecal spermatheca with nephridia, and the first spermathecal pore on segment 4/5. In addition, other characters found on P. darnleiensis were the presence of an annular clitellum on segment XIV-XVI, an epilobus prostomium with open base, approximately 40 single pointed setae on segment XIII, one midventral female pore on segment XIV, one pair of lateroventral male pores on segment XVIII, four pairs of lateroventral spermathecal pores on segment 4/5, 5/6, 6/7, 7/8, and the first middorsal dorsal pore on segment 12/13. The histology of P. darnleiensis showed basic structure as found in other earthworms.
Apis koschevnikovi: Distribution in South Kalimantan and Cytochrome b Mitochondrial DNA Variations Fitriya, Jazirotul; Raffiudin, Rika; Atmowidi, Tri; Hepburn, Randall
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.79 KB) | DOI: 10.14203/jbi.v8i1.3063

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Apis koschevnikovi: Distibusi di Kalimantan Selatan dan Variasinya pada Mitokondria Cytochrome b. Variasi mitokondria berdasarkan Cytochrome b (Cyt b) pada Apis koschevnikovi di lakukan di tujuh lokasi di Kalimantan Selatan Indonesia. Di koleksi 29 koloni A. koschevnikovi yang diperoleh di kawasan hutan primer dan sebagian koloni tidak dijumpai dihutan bekas penebangan. Hasil analisis Cyt b juga mengindikasikan bahwa terjadi variasi genetik dari jenisini walaupun secara morfologi menunjukkan bentuk yang samaKeywords : Apis koschevnikovi, penebangan hutan, variasi haplotipe
Ecology Service Tumbuhan Herba untuk Lebah Trigona sp. ., RISMAYANTI; ., TRIADIATI; RAFFIUDIN, RIKA
Jurnal Sumberdaya Hayati Vol 1, No 1 (2015)
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.%p

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Herbaceous plants are able to flower any time, give them opportunity to interact with insects. This study aimed to analyze the diversity of pollen collected by Trigona sp. in herbaceous ecosystem and counted the percentage of pollen viability from the pollen basket. Trigona’s nest was adapted at plot and the herbaceous plants with their pollen were identified. Pollen were took from hind legs at 07.00, 08.00 and 09.00 am. Pollen slide were make by acetolysis and SEM methods. Pollen were identified and examined of viability. Identification herbaceous plants and their pollen on plot showed that there were 7 herbaceous plants: Kyllinga monocephala, Cleome rutidosperma, Pennisetum polystachyon, Ageratum conyzoides, Brachiaria mutica, Cyperus orodatus, and Eleutheranthera ruderalis. Otherwise pollen which were collected by Trigona sp. consist of 11 kinds of pollen from 6 families, that were Sapindaceae, Leguminoceae, Loranthaceae, Malvaceae, Symplocaceae, and unidentified family.Viability of pollen ranged from 88.8% to 99.8%. The result of identification herbacious plants and pollen that were taken by Trigona sp. indicated that there was not similarity therefore, it was no interaction between herbaceous plants in the plot with Trigona sp.