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RHIZOPUS SPECIES FROM FRESH TEMPEH COLLECTED FROM SEVERAL REGIONS IN INDONESIA Hartanti, Anastasia Tatik; Rahayu, Gayuh; Hidayat, Iman
HAYATI Journal of Biosciences Vol. 22 No. 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2074.192 KB) | DOI: 10.4308/hjb.22.3.136

Abstract

Recent changes in taxonomy of Rhizopus, which are now heavily relying on molecular approach, create significant problem in assigning species name to particular Rhizopus strains isolated from various sources, including tempeh. The present study aims to determine 36 strains of Rhizopus from tempeh originated from 26 locations in Indonesia, using combination of molecular phylogenetic analysis based on internal transcribed spacer ribosomal DNA sequence, physiology, and morphology to species level. The results showed that most of the strains belong to R. microsporusecomplex, and only one strain belongs to R. delemar. Morphological variations within R. microsporus were observed, but under current approach they were insufficient for infraspecific delimitation. The current report is an important contribution in validating the identity of Rhizopus from fresh tempeh in Indonesia.
IMPACT OF DOMESTICATION ON THE ENDOPHYTIC FUNGAL DIVERSITY ASSOCIATED WITH WILD ZINGIBERACEAE AT MOUNT HALIMUN SALAK NATIONAL PARK Putra, Ivan Permana; Rahayu, Gayuh; Hidayat, Iman
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.363 KB) | DOI: 10.4308/hjb.22.4.157

Abstract

Mount Halimun Salak National Park is one of the tropical forest remnants in Java island. The national park has been recognized with high diversity of wild Zingiberaceae. Of that Zingiberaceae, two species namely Alpinia malaccensis (AM) and Horstendia conica (HC), were domesticated as garden plants in the surrounding area of the forest for medicinal use. The impact of domestication on the fungal endophytes associated with these two species of Zingiberaceae is reported here. Fungal endophyte diversity in the wild and domesticated AM and HC was analyzed based on the culturable fungi. Identification of species level used morphological and molecular approaches of ITS rDNA sequence. This study determined 19 species of fungal endophytes, namely Arthrinium malaysianum, Aspergillus flavipes, As. sydowii, Chaetomium globosum, Cladosporium oxysporum, Cladosporium sp., Colletotrichum boninense-complex, Co. cliviae-complex, Co. gloeosporioides-complex, Diaporthe sp., D. anacardii, D. gardenia, Exophiala sp., E. lecanii-corni, Guignardia mangiferae, Ochroconis gallopava, Penicillium citrinum, Pyricularia costina, and unsporulated Sydowiellaceae. Among them, A. malaysianum, C. globosum, Co. cliviae-complex, D. gardenia, and unsporulated Sydowiellaceae were only found in domesticated plants, while some others were absent. Colletotrichum boninense-complex was commonly found in both wild and domesticated plants. Domestication activity affected the diversity of endophytic fungi of AM and HC.
IDENTIFIKASI INFRASPESIFIK FUSARIUM OXYSPORUM ASAL SUBTRAT NONPISANG DAN KEMAMPUAN PINDAH INANGNYA KE TANAMAN PISANG Rahayu, Gayuh; Widodo, Widodo; Mahasari, Ni Putu Winda
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.619 KB) | DOI: 10.14692/jfi.15.1.27

Abstract

Infra-specific Identification of Fusarium oxysporum from Nonbanana Substrates and Its Ability to Move Hosts to Banana PlantsFusarium oxysporum has various life style, i.e. saprobe, endophyte and pathogen. Plant pathogenic F. oxysporum are divided into many forma specialis (f. sp.) depending on the host, for instance F. oxysporum f. sp. cubense (Foc), a causal agent of Panama disease of banana. The study aimed to determine the infraspecific identity of F. oxysporum from non-banana host and evaluate its ability to jump banana plants. Infraspecific identity was determined through a molecular approach using 3 specific primers to recognize TR4 race (TR4 F/R, TR4 F/R1, and FocSc-1/ FocSc-2), while the host?s transfer ability was tested on 2 banana cultivars namely cv. Ambon and Tanduk. Thirteen strain studied  i.e IPBCC 88,012, IPBCC 07,528, IPBCC 07,561, IPBCC 08,562, IPBCC 08,568, IPBCC 10.674, IPBCC 14.1236, IPBCC 14.1237, IPBCC 14.1238 and IPBCC 14.1239 were TR4 Foc; IPBCC 07,338 and IPBCC 14.1242 are race Foc 4. The pathogenicity test of Foc IPBCC 88,012, 07,328, 08,561, 10,674 and 14.1236 derived from cucumbers, soil, agarwood sapwood, insect nests and quinine tree endophytes showed that these strains were able to move to banana plant. This shows that Foc may not host-specific and the infaspecific term forma specialis is therefore debatable.
POPULATION DYNAMICS OF YEASTS AND LACTIC ACID BACTERIA (LAB) DURING TEMPEH PRODUCTION EFRIWATI, .; SUWANTO, ANTONIUS; RAHAYU, GAYUH; NURAIDA, LILIS
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.422 KB) | DOI: 10.4308/hjb.20.2.57

Abstract

Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates.
Alkaloid profile of endophytic Diaporthe spp. from Cinchona calisaya Radiastuti, Nani; Rahayu, Gayuh; Okane, Izumi; Hidayat, Iman; Achmadi, Suminar S.
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v18i1.69

Abstract

Endophytic fungi have been known as potential source of bioactive compound, similar to their host.  This study was conducted to identify endophytic Diaporthe spp. isolated from Cinchona calisaya and to explore their potential in producing alkaloids, particularly quinine. A total of 39 strains of Diaporthe spp. were identified using ITS1-5.8S-ITS2 and EF1-α gene. Seventeen strains have species name and they represent eight species of Diaporthe i.e. D. cynaroidis, D. endophytica, D. ganjae, D. gardeniae, D. litchicola, D. phaseolorum, D. pseudomangiferae, and D. rhoina. The re­maining 22 strains represents 15 groups of unidentified Diaporthe sp. High performance liquid chromatography analyses of chloroform extract of 21-days old static cultures of all fungi in potato dextrose broth showed that these strains produce totally 82 different alkaloids. Each strain producedabout 2-38 different alkaloids. Sixteen out of 39 strains (41%), i.e. one strain each of D. endophytica, D. gar­deniae,  D. phaseolorum, and  D. pseudo­mangiferae, 2 strains of D. litchicola, and 10 strains of unidentified Diaporthe were able to produce quinine. The quinine concentration ranges from 1.1 mg/l to 155.2 mg/l. By using Jaccard’s similarity index 0.44 as the cutting score, UPGMA analyses of alkaloid profile of these strains shows that these strains forms 23 clusters. Alkaloid profile clustering does not support the phylogenetic grouping. This indicates that the alkaloid profile is strain dependent. Some potential strains should be further investigated for optimization of their quinine production.
FUNGI PADA BATANG POHON Paraserianthes falcataria DAN ASOSIASINYA DENGAN Xystrocera festiva (Coleoptera : Cerambycidae) Yunasfi, Yunasfi; Hadi, Soetrisno; Rahayu, Gayuh; Santoso, Teguh
Jurnal Penelitian Hutan Tanaman Vol 6, No 4 (2009): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2009.6.4.251-259

Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis fungi yang berkembang pada batang pohon Paraserianthes falcataria yang diserang oleh Xystrocera festiva. Fungi diisolasi dari kulit dan kayu gubal pada beberapa tahap gerekan X. festiva. Acremonium sp. 1 berhasil diisolasi dari kulit batang. Pada permukaan kayu gubal ditemukan Trichoderma virens dan Acremonium sp. (aff. Acremonium sp. st. imperfect Nectria arenuloides). Trichoderma harzianum dan Acremonium sp. (aff. Acremonium sp. st. imperfect N. cyathea) ditemukan pada terowongan kayu gubal yang dibuat oleh X. festiva. Acremonium spp. juga terdapat pada larva dan imago X. festiva. Hal ini menunjukkan adanya interaksi antara Acremonium spp. dengan X. festiva. Terdapat indikasi bahwa T. virens dan T. harzianum menekan perkembangan Acremonium spp. pada batang pohon P. Falcataria.
Potensi Simbiosis Cendawan Endofit pada Beberapa Tanaman Pangan dan Hortikultura Seviani, DN; Rahayu, Gayuh; Hidayat, Iman
Jurnal Mikologi Indonesia Vol 1, No 1 (2017): Juni 2017
Publisher : Perhimpunan Mikologi Indonesia

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Abstract

Produktivitas tanaman pangan dan hortikultura seperti kedelai, sorgum manis, cabai, dan Chinese cabbage diduga dapat ditingkatkan melalui simbiosis dengan cendawan endofit. Cendawan endofit koleksi IPB Culture Collection strain IYT30, IYT50, IYT64, IYT65, dan IYT102 belum diketahui potensi endosimbiosisnya. Oleh sebab itu penelitian ini bertujuan untuk menguji potensi simbiosis cendawan endofit tersebut terhadap tanaman pangan dan hortikultura yaitu kedelai, sorgum manis, cabai, dan Chinese cabbage pada sistem interaksi kultur aksenik. Endosimbiosis diamati dengan adanya kolonisasi cendawan endofit pada jaringan akar, serta pertumbuhan tanaman inangnya (tinggi tajuk, jumlah daun, panjang akar, dan bobot kering biomassa). Hasil penelitian menunjukkan akar kedelai dan sorgum manis tidak dapat bersimbiosis dengan cendawan endofit pada kondisi uji. Oleh sebab itu pertumbuhan tanaman lebih dipengaruhi faktor-faktor selain simbiosis. Pada akar cabai dan Chinese cabbage, kolonisasi hanya mencapai ruang antar sel jaringan korteks, kecuali pada perlakuan IYT64 pada tanaman cabai dimana kolonisasi sampai ke dalam sel. Tanaman yang diinteraksikan dengan cendawan menunjukkan respon yang tidak konsisten pada semua parameter pertumbuhan. Kata kunci – cendawan endofit – interaksi – kolonisasi – simbiosis
HUBUNAGN KEMAMPUAN PERGANTIAN INANG DENGAN PLASTISITAS GENETIKA PADA CENDAWAN BLAS PADI (PYRICULARIA GRISEA) Listiyowati, Sri; Widyastuti, Utut; Rahayu, Gayuh; Hartana, Alex; Jusuf, Muhammad
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P. grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Bali and Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolates Dc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1.
Uji Simbiosis Kapang Endofit pada Tanaman Chinese cabbage (Brassica rapa) dan Cabai (Capsicum annuum) Hapipah, R; Rahayu, Gayuh; Hidayat, Iman
Jurnal Mikologi Indonesia Vol 2, No 1 (2018): Juni 2018
Publisher : Perhimpunan Mikologi Indonesia

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Abstract

Kapang endofit berperan membantu tanaman inang beradaptasi pada lingkungan yang kurang mendukung, dan membantu penyerapan nutrisi sehingga memperbaiki pertumbuhan inang melalui berbagai mekanisme.Penelitian ini bertujuan untuk menguji potensi simbiosis lima kapang endofit akar koleksi IPBCC (IYT11, IYT23, IYT42, IYT72, dan IYT84) dengan tanaman chinese cabbage dan cabai. Simbiosis diuji pada media Oat Meal Agar (OMA) dan zeolit selama 3 minggu. Potensi simbiosis diamati melalui kolonisasi akar dan respon tumbuh tanaman (tinggi tajuk, panjang akar, jumlah daun, dan bobot kering biomassa tanaman). Kolonisasi akar diamati menggunakan teknik pewarnaan biru tripan 0,005%. Hasil uji menunjukkan bahwa kelima isolat tidak mengkolonisasi akar chinese cabbage, namun mampu mengkolonisasi akar cabai pada medium OMA. Hasil uji pada medium zeolit menunjukkan bahwa isolat Leohumicola sp. IYT23 and Hortea sp. IYT42 mampu mengkolonisasi akar tanaman cabai, tetapi tidak meningkatkan pertumbuhan tanaman.