Mulyorini Rahayuningsih
Department of Agroindustrial Technology, Faculty of Agricultural Techonology, Bogor Agricultural University, Darmaga Campus, Bogor, 16680

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DIRECT ETHANOL PRODUCTION FROM BREADFRUIT STARCH (ARTOCARPUS COMMUNIS FORST.) BY ENGINEERED SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (ESSF) USING MICROBES CONSORTIUM Farida, Iftachul; Syamsu, Khaswar; Rahayuningsih, Mulyorini
International Journal of Renewable Energy Development Vol 4, No 1 (2015): February 2015
Publisher : Center of Biomass & Renewable Energy, Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (879.554 KB) | DOI: 10.14710/ijred.4.1.25-31

Abstract

Breadfruit (Artocarpus communis Forst.) is one of sources for ethanol production, which has high starch content (89%). Ethanol production from breadfruit starch was conducted by Simultaneous Saccharification and Fermentation (SSF) technology using microbes consortium. The aim of the research was to examine a method to produce ethanol by SSF technology using microbes consortium at high yield and efficiency. The main research consisted of two treatments, namely normal SSF and enginereed SSF. The results showed that normal SSF using aeration and agitation during cultivation could produce ethanol at 11.15 ± 0.18 g/L, with the yield of product (Yp/s) 0.34 g ethanol/g substrate; and yield of biomass (Yx/s) 0.29 g cell/g substrate, respectively. A better result was obtained using engineered SSF in which aeration was stopped after biomass condition has reached the end of the exponential phase. The ethanol produced was 12.75 ± 0.04 g/L, with the yields of product (Yp/s) 0.41 g ethanol/g substrate, and the yield of cell (Yx/s) 0.09 g cell/g substrate.
PENGOLAHAN LIMBAH CAIR PERIKANAN MENGGUNAKAN KONSORSIUMMIKROBA INDIGENOUS PROTEOLITIK DAN LIPOLITIK Oktavia, Devi Ambarwaty; Mangunwidjaja, Djumali; Wibowo, Singgih; Sunarti, Titi Candra; Rahayuningsih, Mulyorini
AGROINTEK Vol 6, No 2 (2012)
Publisher : University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v6i2.1975

Abstract

Fish processing industries produce liquid waste at different capacity in every level from production line. Because of less attention and knowledge, wastes become worse. The aim of this study was to discover potential microbial consortium that can degrade protein and fat of liquid waste from fish production processing. The consortiums formula were chosen from bacteria that  have the highest specific growth. Formulation A which degraded soluble protein than others. Formulation of B which degraded fat than others. All formulation had pH in range are 6 - 9. Liquid waste which outoclaved had degraded protein, soluble protein and pH parameters
KARAKTERISTIK KEFIR DENGAN PENAMBAHAN PUREE UMBI GEMBILI Kartika, Kartika; Rahayuningsih, Mulyorini; Setyaningsih, Dwi
EDUFORTECH Vol 4, No 2
Publisher : Program Studi Pendidikan Teknologi Agroindustri, UPI (Universitas Pendidikan Indonesia)

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Abstract

Kefir merupakan produk susu yang beraroma asam, alkoholik, karbonat, dan dibuat melalui proses fermentasi menggunakan mikroba bakteria dan yeast. Kefir merupakan probiotik alami yang mengandung bakteri hidup yang dapat memberikan efek menyehatkan bagi tubuh manusia apabila dikonsumsi. Umbi gembili mengandung inulin yang dapat dimanfaatkan sebagai prebiotik. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan puree gembili dan waktu fermentasi pada nilai pH, total asam tertitrasi, total gula dan gula pereduksi, serta pertumbuhan bakteri asam laktat dan khamir pada kefir. Metode yang digunakan dalam penelitian ini adalah eksperimen dengan analisis data deskriptif. Hasil dari penelitian ini menunjukan bahwa penambahan puree gembili sebanyak 4% dapat menurunkan nilai pH, meningkatkan total asam tertitrasi, meningkatkan total gula dan gula pereduksi, serta meningkatkan pertumbuhan bakteri asam laktat dan khamir dibandingkan kefir kontrol. Lama waktu fermentasi kefir puree gembili terbaik adalah selama 24 jam dengan nilai pH 4,17; total asam tertitrasi 0,64%; bakteri asam laktat 8,991 log CFU/ml dan khamir 8,579 log CFU/ml.
Bioinsecticides Production by Bacillus thuringiensis subsp. aizawai using Agroindustrial by-Product in Solid Fermentation Sasmitaloka, Kirana Sanggrami; Sunarti, Titi Candra; Rahayuningsih, Mulyorini
Jurnal Penelitian Pascapanen Pertanian Vol 13, No 1 (2016): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v13n1.2016.1-10

Abstract

Bacillus thuringiensis subsp. aizawai is one of well-known bioinsectiside resources, and utilized in organic farming for the replacement of chemical insecticide. The active compounds produced from fermentation process of B. thuringiensis subsp. aizawai are bacterial spores and protein crystal of δ-endotoxins, which are variously toxic to larvae of the Lepidoptera and Coleoptera. The use of bioinsecticides in Indonesia is still rarely because bioinsecticide marketed in Indonesia is still an import product so that price is relatively expensive. This problem can be overcome with producing bioinsecticide contain active B. thuringiensis using agroindustrial by product as a raw materials. This research compared the bioinsecticide production from several agroindustrial by product in solid fermentation of B. thuringiensis subsp. aizawai. This research aimed to find out the ability of B. thuringiensis subsp. aizawai using substrate combination of carbon source (cassava dregs, pulp of coffee, starch fractions of iles-iles, and sago dregs) and nitrogen source (tofu’s waste, peanut meal, palm kernel cake, and corn hominy) in bioinsecticides production using solid media cultivation. The research consisted of three stages, there were substrates characterization, microorganisms characterization, and bioinsecticides production. The main parameters to select the carbon and nitrogen sources are LC50 value and potential bioinsecticides products. The results showed that the best cultivation of B. thuringiensis subsp. aizawai contained in combination cassava dregs and palm kernel cake blends. This cultivation produced total cell counts 11.2 log CFU/g, viable spore counts 8.9 log CFU/g, LC50 0.04 μg/ ml and potential products 20000 IU/mg. Biopesticide produced can be used to kill Crocidolomia binotalis on cabbage. PRODUKSI BIOINSEKTISIDA OLEH Bacillus thuringiensis subs. aizawai PADA KULTIVASI MEDIA PADAT MENGGUNAKAN LIMBAH AGROINDUSTRI Bacillus thuringiensis subsp. aizawai merupakan salah satu bakteri yang digunakan untuk menghasilkan bioinsektisida dan digunakan pada pertanian organik untuk menggantikan pemakaian insektisida kimia. Komponen aktif yang diproduksi dari proses fermentasi B. thuringiensis subsp.aizawai adalah spora dan kristal protein δ-endotoksin, yang bersifat toksin terhadap larva Lepidoptera dan Coleoptera. Penggunaan bioinsektisida di Indonesia masih jarang karena biasanya berupa produk impor yang harganya mahal. Permasalahan ini dapat diselesaikan dengan memproduksi bioinsektisida menggunakan bahan aktif B. thuringiensis dan hasil samping agroindustri sebagai bahan baku. Penelitian ini bertujuan mengkaji kemampuan produksi B. thuringiensis subsp. aizawai pada beberapa jenis substrat sumber C (onggok, kulit kopi, fraksi pati iles-iles, dan ela sagu) dan sumber N (ampas tahu, bungkil kacang tanah, bungkil inti sawit, dan ampok jagung) dalam memproduksi bioinsektisida dari limbah agroindustri. Penelitian ini terdiri dari tiga tahap, yaitu karakterisasi substrat, karakterisasi isolat dan produksi bioinsektisida. Parameter utama untuk menetapkan sumber karbon dan nitrogen yang tepat adalah nilai LC50 dan potensi produk bioinsektisida. Hasil penelitian menunjukkan bahwa produksi bioinsektisida yang terbaik terdapat pada kultivasi B. thuringiensis subsp. aizawai menggunakan kombinasi onggok dan bungkil inti sawit. Pada kultivasi ini dihasilkan jumlah sel hidup 11.2 log CFU/g, jumlah spora 8.9 log CFU/g, nilai LC50 0.04 μg/ ml dan potensi bioinsektisida 20000 IU/mg. Bioinsektisida yang dihasilkan dapat digunakan untuk membasmi larva Croccidolomia binotalis pada tanaman kubis.
PENGGANDAAN SKALA PRODUKSI BIOINSEKTISIDA BACILLUS THURINGIENSIS VAR. ISRAELENSIS UNTUK MEMBASMI JENTIK NYAMUK AEDES AEGYPTI Rahayuningsih, Mulyorini; Syamsu, Khaswar; Darwis, Abdul Aziz; Purnawati, Rini
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.727 KB)

Abstract

The objective of this research is to study the scaling up of bionsecticide production from Bacillus thuringiensisvar. israelensisusing onggok (a cassava by-product) as a carbon source. The insecticide produced was used to eradicate Aedes aegypti larvae. The product was a crystal protein produced during bacterial sporulation. Scaling up from laboratory to pilot plant scale was done using two methods, i.e. constant agitation power per unit volume (Pg/V) and constant oxygen transfer coefficient (kLa). The results showed that yield of product per substrate (Yp/s) of Pg/V based product with the value of 3.52 ± 0.02 spora per gram substrates was higher than Yp/s of  kLa based product with the value of 2.96 spora per gram substrate.  Logarithmic value of viable spore count (log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as compared to 7.17 ± 0.20 for kLa based product. Substrate efficiency was also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of Pg/V based product was lower (0.49 ?g/ml) meaning that it was more toxic than kLa based product (0.62 ?g/ml). Amino acid content of Pg/V based product was also higher than kLa based product. Constant Pg/V method was suggested as a based on the scaling up of bioinsecticide production of B. thuringiensis israe/ensison industrial scale. Keywords: bioinsecticide, Bacillus thuringiensisvar. israelensis, kLa, Pg/V, LC50, viable spore count