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SISTEM KULTIVASI ORGANIK PADA KACANG TANAH (Arachis hypogea L.) DENGAN MEMANFAATKAN TINGKAT KEMATANGAN KOMPOS Agustiyani, Dwi; Martono, Budhi; Rahmansyah, Maman
Jurnal Teknologi Lingkungan Vol. 13 No. 2 (2012)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.875 KB) | DOI: 10.29122/jtl.v13i2.1419

Abstract

Untuk mendukung suatu sistem pertanian yang berkelanjutan, praktek pertanian organic dilakukan pada kultivasi kacang tanah. Pada penelitian ini, pengkombinasian antara penggunaan bakteri pelarut fosfat (Pseudomonas sp.), bakteri penambat nitrogen (Rhizobium spp.), dan fungi pendegradasi selulosa (Trichoderma sp. dan Aspergillussp.) digunakan untuk inokulan. Kompos berupa bahan hijauan daun dan limbah kotoran ternak kemudian diformulasikan dengan inokulan untuk digunakan sebagai pupuk hayati. Kompos-matang dan kompos-setengah-matang fermentasi digunakan untuk pengkayaan hara tanah. Penggunaan kompos setengah matang merupakanpraktek semi-on-site-composting di dalam upaya menstimulasi pertum-buhan tanaman. Pengaruh perlakuan pada penelitian ini dievaluasi melalui pertum-buhan tanaman dan hasil polong. Tidak didapat perbedaan yang signifikan di antara perlakuan pupuk hayati, namun seluruh perlakuan memperlihatkan perbedaan yang nyata terhadap kontrol.Oleh sebab itu, praktek pengunaan kompos setengah matang mampu mempersingkat persiapan tanam dan dapat diterapkan pada kultivasi kacang tanah. Penggunaan kompos separuh matang hasil penelitian ini dapat dipraktekan di dalam pengembangan tehnik kultivasi untuk mendukung sistem pertanian berkelanjutan. Kata kunci : pupuk hayati, semi-on-site-composting, sistem pertanian berkelanjutan, Arachis hypogea L. AbstractIn order to support a sustainable agriculture system, an organic farming purpose had been carried out all through groundnut cultivation. In this experiment, the combination of phosphate solubilizing bacteria (Pseudomonas sp.), fixing nitrogen bacteria (Rhizobium spp.), and fungal degrading cellulose (Trichoderma sp. and Aspergillus sp.) were used as inoculant. Organic substrate as a green manure compost and cattle waste was mixing with the inoculant to utilize into biofertilizer improvement. Full and half fermented compost materials designed for soil augmentation. Half fermented compost materials exploited to semi-on-site-composting practice in appropriate to fuel plant growth. Result of the treatment evaluated through plant growth and the pods yield. There have not significant  different among biofertilizer handling, and all have the exclusion to the control. As due
AKTIVITAS FOSFATASE TANAH DILINGKUNGAN BENTANG HUTAN ALAMI DAN NON-ALAMI Suliasih, Suliasih; Rahmansyah, Maman
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.287 KB) | DOI: 10.14203/beritabiologi.v9i6.856

Abstract

The spatial dispersal of acid and alkaline phosphates activity in forest soils appears to be controlled by position in the landscape and its soil microbial density. Soil bacteria expressively produced significant level of acid phosphatase in the investigation.The variety of acid phosphatase activity (8.25-37.55 />-nitrophenol.g"soil.h") noted higher and followed by alkaline (0.78-7.15 p-nitrophenol.g-soil.h" ), correspondingly. Acid and alkaline phosphates were exist in both of soil of natural as well as for non-natural forest soil landscape, with the value (po/o=0.6210) of correlation are 0.6889 and 0.6532, respectively. Phosphate Solubilizing Bacteria (PSB) average density in natural forest soil equivalent to 0.61xl0Â colony forming unit (cfu) and total bacteria is 160xl0 cfu, while the PSB in non-natural forest soil is 7.75xl0 cfu and total bacteria is 48.25x10 cfu. Concerning to the forest environment as soil bacteria inhabitants of PSB, there were significantly difference of PSB population in natural and non-natural landscape forest. Forest environment is negatively affect the soil enzymes activities under Pinus maritima and Caliandra calothyrsus as the lowest activities, and to the highest ones under the Schima wallichii and Eucalyptus saligna vegetation.
KARAKTER RESPIRASI DAN MINERALISASI KARBON ORGANIK PADA SAMPEL TANAH DIKOLEKSI DARI PULAU BANGKA [Respiration and Organic Carbon Mineralization Character in Soil Samples Collected from Bangka Island] Rahmansyah, Maman; Suliasih, Suliasih
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3877.84 KB) | DOI: 10.14203/beritabiologi.v15i1.2855

Abstract

The study was designed to explore soil biomass content and soil enzymatic activities that involved in carbon organic soil mineralization. Samples of soil were collected from two locations in Bangka Island. Bulk samples of top soil (TP) and excavated (TG)  were collected from garden soil in Pangkalpinang. Other soils were gathered from forest floor (TU), beneath pelawan (Tristaniopsis merguensis Griff.) trees which is endemic plant to North Bangka forest. Soil biological character was evaluated by measuring soil microbial population, respiration rate, and cellulase and amylase activities. Experiments were carried out in Research Center for Biology, Indonesian Institute of Sciences, on dried soil treatment (Control), soil moistened with 5 mL H2O in 100g soil (Gluc.0), and soil moistened with 5 mL 1% glucose solution (Gluc.1). Bacterial population obtained was higher than fungal population in all soil samples. Soil respiration activity in TU after treatment was 4.51±0.49; 5.26±0,79; and 8.28±1.41 ppm CO2/100g-soil/5-minutes, respectively. Meanwhile , respiration in TP  were 3.65 ± 2.12; 3.57 ± 1.18; and 7.94 ± 1.05 ppm CO2/100g-soil/5-minutes; and in the TG are 2.61 ± 0.70; 3.34 ± 0.94; and 5.46 ± 2.30 ppm CO2/100gsoil/5-minutes. Cellulase activity of all samples were not significantly different. Forest soil cellulase activity was significantly different compared to garden soil. Glucose induction was positively increased amylase activity compared to cellulase. Soil enzyme, soil respiration activities and microbial population of forest soil could be used as a reference to recover degraded land in Bangka Island.
Pengaruh Inokulasi Bakteri Terhadap Pertumbuhan Awal Jarak Pagar (Jatropha curcas L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

Abstract

ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.
Ragam Aktivitas Urease dan Fosfomonoesterase serta Perannya dalam Ketersediaan Nutrisi N dan P pada Tanah Kebun Biologi Wamena Rahmansyah, Maman; Latupapua, H.J.D.; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.513 KB) | DOI: 10.14203/jbi.v3i4.3325

Abstract

ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.
Pseudozyma aphidis as inoculant for local chicken Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.679 KB) | DOI: 10.14203/jbi.v12i1.2327

Abstract

SHORT COMMUNICATION
Pertumbuhan Bakteri Hasil Isolasi dari Tanah Perkebunan yang Tumbuh pada Media Mengandung Pestisida Propoksur dan Karbaril Imamuddin, Hartati; Laili, Nur; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.636 KB) | DOI: 10.14203/jbi.v8i2.3054

Abstract

Growth Capacity of Soil Bacteria Isolates Obtained from Estate Area from Media Containing Propoxur and Carbaryl. Isolation actions have been carried out with three soil samples derived from soil estate of pine apple, sugar cane, and banana in Lampung area, Southern Sumatra. The isolates were grown in the selected media containing carbaryl insecticide, and herbicide of diuron and bromocyl. The highest and vigorous growth isolates have gathered from threedifferent soil samples, and subsequently named GGPC, GM, and NTF isolates. Furthermore, those isolates were studied through the growth rate in the media containing propoxur and carbaryl in the nutrient rich liquid (NB) and compared to limited mineral (MM). All of the isolates grew faster against pesticides in the liquid medium of NB compared to MM. High correlated value among parameters nurtured as due to bacterial population and those processes were not affected by media composition. The GM isolate showed similar pattern during propoxur decrease along with incubation even though the rate of pesticide elimination from the media was higher in the rich nutrient liquid media (NB) compared to MM.Keywords: microbe isolate, pesticide, propoxur, carbaryl
Pengaruh Pemberian Kompos dan Pupuk Hayati terhadap Aktivitas Fosfatase dan Urease pada Tanah yang Ditanami Brokoli (Brassica oleracea L.) Rahmansyah, Maman; Antonius, Sarjiya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2163

Abstract

Organic farming basically work on improving organic soil substrate which give impact to plant growth promoting bacteria. On the other hand, the use of agrochemicals leave chemical residues in the soil. In an effort to get the quality of organic matter and selected microbes as a biofertilizer material, this research had been done. Half ripe composting (HRC) process of organic substrate was utilized to enrich soil containing agrochemical residue, while ripe compost (RC) substance amended to soil free residue. That compost was used in broccoli cultivation. Microbial degrading pesticide was added to compost as biofertilizer formula. Even though plant biomass and the yield of flower was unclearly affected, but moreover the HRC application as due to “semi-on-site-composting” practices in soil containing agrochemical residue would cut off compost processing time. Soil microbial activity measured through soil respiration, urease and phosphomonoesterase indicated that the value was lower in samples collected from the soil free residue compared with soils containing residues. Utilization of chicken manure resulted in the availability of high nitrogen compounds and causing imbalance absorption by plants, compared with the use of HRC and RC in this study. Keywords: soil respiration, phosphatase, urease, Brassica oleracea L. 
Optimasi Enzim ?-Amilase dari Bacillus amyloliquefaciens O1 yang Diinduksi Substrat Dedak Padi dan Karboksimetilselulosa Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1523.768 KB) | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
Production of Acid Phosphatase in Bacillus sp. Isolated from Forest Soil of Gunung Salak National Park Rahmansyah, Maman; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3140

Abstract

ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.