Erdiansyah Rahmi
Fakultas Kedokteran Hewan, Universitas Syiah Kuala

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EFFECT OF STORAGE TIME OF EPIDIDYMIS AT 5 OC ON SPERMATOZOA QUALITY OF ACEH LOCAL GOAT Hamdan, Hamdan; Panjaitan, Budianto; Sutriana, Amalia; Aliza, Dwinna; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.236 KB) | DOI: 10.21157/j.ked.hewan.v4i2.9811

Abstract

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P 0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.
THE DISTRIBUTIONS OF GLYCOGEN IN THE STOMACH ON LONG TAILED MACAQUE (MACACA FASCICULARIS) DURING PRE AND POSTNATAL PERIOD Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2447.781 KB) | DOI: 10.21157/j.ked.hewan.v2i2.9785

Abstract

The study was done to investigate the glycogen distribution in the stomach of long tailed macaques (Macaca fascicularis). The study used eight individuals of long tailed macaques consist of 6 fetuses (55, 70, 85, 100, 120, and 150 days pregnancy) and 2 infants (10 and 105 days). The gastrict tract were histologically stained using Hematoxyllin-Eosin (H E), and histochemically stained using Periodic Acid Schiff (PAS), to detect glycogen. The results revealed that the histogenesis pattern of stomach glandullar cells are developed in line with the age of the fetuses and infant. We also found that glycogen existance are increase in line with the age in the glandullar stomach. From the present study we concluded that the glycogen distribution in gastrict glandular cells were influenced by the age in the fetal period, while in the infant was under influence of the digestion activities, and the activities of the gastrict glandular cells was already seen at 55 days gestation.
IN VITRO ANTIMALARIAL ACTIVITY OF JALOH LEAVES EXTRACT ON PLASMODIUM FALCIPARUM Asmilia, Nuzul; Sutriana, Amalia; Rahmi, Erdiansyah; Sugito, .
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Malaria still cause health problem in tropics including Indonesia, since its generate high mortality rate. Medication of Malaria has been performed long time ago without optimum result. One reason is the high rate of P.falciparum resistance on conventional antimalaria drug. This research aim to evaluate the activity of jaloh leaves extract (Willow leaves) on the inhibition of P.falciparum in vitro.This was a laboratory experimental research that using Indonesian isolates of cloroquin resistance P.falciparum obtained from NAMRU-2, Jakarta. P.falciparum isolate that has been synchronized were divided in to 3 groups of treatment : Jaloh leaves ethanolic extract, jaloh leaves ethyl acetate extract, and jaloh leaves n-hexane extract. Each group were then alloted into 5 dosage levels: 6,25ug/ml; 12,50ug/ml; 25,00ug/ml; 50,00ug/ml; and 100ug/ml. The observation of parasitemia degree were done 48 hours post-incubation in which the thick blood smear were colored with Giemsa and then examined using bioculer microscope. Data were analyzed using one way ANOVA and Newman-Keuls test. The result showed that the percentage of P.falciparum growth inhibition were sinificantly different (P 0.05) among treatments wherein ethanol extract and ethyl acetate extract more inhibit P.falciparum growth than the hexane extract. The administration of different dosage also resulted in significance different (P 0.05) of P.falciparum growth inhibition. This research concludes that jaloh leaves extract could inhibit P.falciparum growth.
Isolation and Identification of Genus Salmonella and Shigella from Sumatran Orangutan (Pongo abelii) Feces in Orangutan Reintroduction Center, Jantho Rahmi, Erdiansyah; Agustina, Dina; Jamin, Faisal
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.503 KB) | DOI: 10.21157/j.med.vet..v8i1.3316

Abstract

This study was done to identified bacteria of genus Salmonella and Shigella from sumatran orangutan (Pongo abelii) feces. Nine semi wild orangutan fecal samples were obtained in Orangutan Reintroduction Center, Cagar Alam Pinus Jantho, Aceh Besar. The samples were processed using Carter methode. The selective media used were Salmonella Shigella Agar (SSA), Eosin Methylen Blue (EMB), and MacConkey Agar. The separated colonies which grawn in SSA media were observed for their morphology, and followed by Gram staining methode then continued with biochemical test such as Indol, Methyl Red (MR), Voges Proskauer (VP), Sulfid Indol Motility (SIM), Simmons Citrate (SC), and sugar test such as glucose, sucrose, lactose, and manitol. From nine samples, one orangutan was infected with Salmonella and another one was infected with Shigella. All of the samples were identified with Escherichia. In Conclusion, orangutan lived at Reintroduction Center, Jantho infected with Salmonella and Shigella bacteria.Key words: feces, orangutan, Salmonella, Shigella
Analysis of the Role of Keeper as a Risk Factor for Infection of Gastrointestinal Nematodes in Sumatran Orangutan in the Re-introduction Station of Jantho Murtala, Ali; Ferasyi, T. Reza; Rahmi, Erdiansyah
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.335 KB) | DOI: 10.21157/j.med.vet..v8i2.3363

Abstract

This study was aimed to identify the role of keeper as a risk factor for the infection of gastrointestinal nematodes on orangutans in Jantho Re-introduction Station. This study was conducted using a census method. A number of 8 orangutan’s keepers were involved as respondent. The data was collected by interview using a structural questionnaire. In addition, feces of 6 orangutans caged in this location were taken as sample. The feces sample was then examined for gastrointestinal nematodes using native and centrifugation methods in Parasitology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University. The data obtained from this study were analyzed descriptively. The results of study by questionnaire showed that a possible potential risk factor for the infection of gastrointestinal nematode to orangutans in Jantho Re-introduction Station is the keeper avoids using gloves when supplying food to the animals. Then, the result of laboratory examination showed that it was confirmed that the feces of orangutans infected with gastrointestinal nematodes, which the prevalence of 16.5% for infection by Strongiloides sp. In conclusion, orangutans caged in Jantho Re-introduction Station are in a low infection of gastrointestinal nematodes of Strongiloides sp., and the keeper shows a good behavior to prevent gastrointestinal nematodes infection to orangutan.Key words: keeper, Sumateran orangutan, Pongo abelii, Strongyloides sp.
PENGARUH EKSTRAK ETANOL DAUN JALOH (SALIX TETRASPERMA ROXB) TERHADAP PERSENTASE PARASITEMIA PADA MENCIT (MUS MUSCULUS) YANG DIINFEKSI PLASMODIUM BERGHEI Asmilia, Nuzul; Sugito, Sugito; Rahmi, Erdiansyah; Febrianto, Niko
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.86 KB) | DOI: 10.21157/j.ked.hewan.v4i1.9796

Abstract

This study aimed to verify the effect of ethanol extract of jaloh leaves at various doses on parasitemia percentage of in mice (Mus musculus), wich inoculated Plasmodium berghei. This study used 12 male mice (Mus musculus) strains Balb / C with average body weight of 30.2 grams of 2-month-old. This research using Completely Randomized Design (CRD) with four treatments and three replications. All the mice were infected with Plasmodium berghei prior to treatment on three days post infected the mice in group 2,3 and 4 were given with extract ethanol jaloh leaves at dose 50, 100, 150 mg/kgbw inspectively. The mice in group 1 (K0) were not given anything and consident as control.Observations parasitemia percentage using microscopy on preparations stained thin blood smear Giemsa. The parasitemia percentage data were analyzed using one way ANOVA. On the average (±SD) percentage of parasitemia during the three days in four treatments (K0, K1, K2, and K3) on the first day were 24.87±1.86; 24.33±1.53; 24.20±1.06; and 24.93±1.01. On the second day were 22.67±2.52; 23.00±1.00; 22.80±1.20; and 24.13±2.01; and on the third day were 24.77±1.66; 22.97±3.76; 21.13±1.90; and 24.27±1.42. Statistical analysis showed that ethanol extract of the jaloh leaves for KO, KI, KII, and KIV respectively treatment did not affect the percentage of parasitemia in mice (Mus musculus) inoculated Plasmodium berghei.
ISOLATION OF ENTEROBACTERIACEAE BACTERIA SPECIES FROM FECES OF SUMATRAN ORANGUTANS (PONGO ABELII) Darmawi, .; Rahmi, Erdiansyah; Dewi, Maryulia; Hutabarat, Joharsyah; Abrar, Mahdi; Fakhrurrazi, .
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The porpouse of this research aimed to isolate enterobacteriaceae bacteria from feces of Pongo abelii. The samples of feces were collected from 15 captive orangutans in Orangutan Sumatera Batu Mbelin Sibolangit Qurantine, North Sumatra. Of each sample was cultured in nutrient broth media using sterilecotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on Methylene Blue Agar (Oxoid), examined by Gram staining, and tested by biochemically. The result showed that significantly more common appear Escherichia sp. (93,33%) and fewer Edwardsiella sp. (66,67%) wereisolated from feces samples of P. abelii. Others enterobacteriaceae found in feces of P. Abelii were Shigella sp. (46,67%), Klebsiella sp. (33,33%), Citrobacter sp., and Salmonella sp. (13,33%), respectively
THE EFFECT OF MALACCA LEAVES (PHYLLANTUS EMBLICA) ETHANOLIC EXTRACT ON PLASMODIUM FALCIPARUM GROWTH IN VITRO Asmilia, Nuzul; TR, T Armansyah; Aliza, Dwinna; Melia, Juli; Rahmi, Erdiansyah; Daulay, Lingga Surya Maret
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.356 KB) | DOI: 10.21157/j.ked.hewan.v12i4.10215

Abstract

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P 0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P 0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.
INHIBIN B MENURUNKAN KONSENTRASI FOLLICLE STIMULATING HORMONE (FSH) PADA TIKUS PUTIH (RATTUS NORVEGICUS): UPAYA PENGEMBANGAN KONTRASEPSI HORMON PRIA BERBASIS PEPTIDA Akmal, Muslim; A, Aulanni?am; Widodo, M. Aris; Sumitro, Sutiman B.; Purnomo, Basuki B.; Siregar, Tongku Nizwan; Hambal, Muhammad; A, Amiruddin; S, Syafruddin; Aliza, Dwinna; Sayuti, Arman; Adam, Mulyadi; Armansyah, T.; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.745 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2788

Abstract

Penelitian ini bertujuan mengetahui efek injeksi inhibin B terhadap penurunan konsentrasi follicle stimulating hormone (FSH) di dalamserum pada tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 24 ekor tikus putih berjenis kelamin jantan dengan strain Wistar berumur 4 bulan dengan bobot badan 150-200 g. Tikus-tikus dikelompokkan secara acak ke dalam 4 kelompok, yaitu KK0, KP1, KP2, dan KP3, masing-masing kelompok terdiri atas 6 ekor. Kelompok KK0 merupakan kelompok kontrol hanya diinjeksi dengan phosphate buffer saline (PBS), sedangkan kelompok KP1, KP2, dan KP3 diinjeksi dengan inhibin B dengan dosis berturut-turut 25, 50, dan 100 pg/ekor. Injeksi inhibin B dilakukan secara intraperitoneum sebanyak 5 kali selama 48 hari dengan interval waktu 12 hari. Injeksi pertama inhibin B dilarutkan dengan0,05 ml PBS dan 0,05 ml Freud?s complete adjuvant (FCA). Injeksi kedua sampai kelima, inhibin B dilarutkan dengan 0,05 ml PBS dan 0,05 ml Freud?s incomplete adjuvant (FICA). Pada hari ke-6 setelah injeksi inhibin B terakhir, tikus dikorbankan secara dislocatio cervicalis,lalu darah dikoleksi langsung dari jantung dan didiamkan hingga didapatkan serum untuk pemeriksaan konsentrasi FSH dengan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa injeksi inhibin B dengan dosis 100 pg/ekor menurunkan konsentrasi FSH secara nyata (P 0,05) bila dibandingkan dengan kelompok kontrol. Berdasarkan hal tersebut, inhibin B berpeluang untuk dikembangkan sebagai kandidat kontrasepsi pria hormon berbasis peptida.
CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD Syafruddin, Syafruddin; Rizal, Khairul; Rosmaidar, Rosmaidar; Melia, Juli; roslizawaty, roslizawaty; Zuhrawati, Zuhrawati; Rahmi, Erdiansyah; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.248 KB) | DOI: 10.21157/j.ked.hewan.v11i3.8687

Abstract

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2? (PGF2?, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.
Co-Authors . Darmawi . Fakhrurrazi . Sugito a, Aulanni?am Abdullah Hamzah, Abdullah Al Azhar Ali Murtala, Ali Amalia Sutriana Amiruddin A Amiruddin Amiruddin Arman Sayuti Awaluddin Awaluddin Azani, Wahyuni Azhar Azhar Azhari Azhari Basuki B. Purnomo budianto panjaitan Chairun Nisa Cut Dahlia Iskandar Darmawi Darmawi Dasrul Dasrul Daulay, Lingga Surya Maret Dian Masyitha Dina Agustina, Dina DONDIN SAJUTHI Dwina Aliza Dwinna Aliza Emha, Risfi Febrina Tri Utami Erni Sulistiawati Faisal Jamin Febrianto, Niko Gholib Gholib, Gholib Habiburrahman Habiburrahman, Habiburrahman Hamdan Hamdan Hamny - Herrialfian, Herrialfian Hery Wijayanto Idawati Nasution Ismail Ismail ITA DJUWITA Jalaluddin, M Joharsyah Hutabarat Juli Melia Khairiah Khairiah M. Aris Widodo M. Hasan M. Isa M. Jalaluddin, M. Mahdi Abrar Maryulia Dewi Masyita, Dian Muhammad Hambal Muhammad Hanafiah Muhammad Isa Mulyadi Adam, Mulyadi Muslim Akmal Mustafa Sabri Nazif, Rahmat Nofri Alfi, Nofri NURLIANA NURLIANA Nuzul Asmilia Pertiwi, Sedrisa Lidya Putri Dewi Ramadhan, Rezky Razali Daud Razali Razali Rinidar Rinidar, Rinidar Rizal, Khairul Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Rusli Rusli Rusli Sulaiman Safika, Safika Sarayulis, Sarayulis Siti Hajar Sr, Meuthya Srihadi Agungpriyono Sugito s Sugito Sugito Sutiman B. Sumitro Syafruddin s Syafruddin Syafruddin Syahputra, Yandi T. Armansyah T. Reza Ferasyi, T. Reza Teuku Reza Ferasyi Tongku Nizwan Siregar TR, T Armansyah Tri Wahyu Pangestiningsih Triva Murtina Lubis Ulfa, Anisa Diah Ummu Balqis Veronica, Vina Wahono Esthi Prasetyaningtyas Wenny Novita Sari Wisnu Nurcahyo Zainuddin Zainuddin Zuhrawati Zuhrawati, Zuhrawati