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AKTIVITAS LARVASIDA BIJI BENGKUANG SEBAGAI INSEKTISIDA NABATI TERHADAP LARVA LALAT CRYSOMYA BEZZIANA Mustika, Aulia Andi; Hadi, Upik Kesumawati; Wardhana, April Hari; Rahminiwati, Min; Wientarsih, Ietje
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.789 KB) | DOI: 10.29244/avi.4.2.68-73

Abstract

Bengkuang merupakan salah satu tanaman obat yang berpotensi sebagai bioinsektisida. Penelitian ini bertujuan mengetahui efektivitas biji bengkuang sebagai insektisida nabati terhadap larva lalat Crysomya bezziana (C. bezziana) agen penyebab miasis secara in vitro. Penelitian ini terbagi menjadi 5 kelompok perlakuan. Masing-masing sebanyak 20 Larva instar 1 (L1), Larva instar 2 (L2), dan Larva instar 3 (L3) C.bezziana digunakan untuk pengujian in vitro menggunakan pot plastik yang berisi media larva dan ekstrak ethanol biji bengkuang dengan konsentrasi bertingkat 0,06, 0,12, dan 0,25%. Coumaphos 0,06% dan akuades steril digunakan sebagai kontrol positif dan negatif. Hasil penelitian menunjukkan bahwa pada konsentrasi 0,25% mampu menyebabkan 100% kematian larva dan 100% pupa tidak menetas. Pengujian L3 menunjukkan bahwa ekstrak ethanol biji bengkuang mampu menyebabkan penurunan daya tetas pada semua konsentrasi. Pengujiaan L1 dan L2 untuk mengindikasikan efektifi tas ekstrak sebagai racun perut, sedangkan pengujian pada L3 sebagai indikasi racun kontak. Biji bengkuang memiliki daya larvasida terhadap beberapa jenis larva serangga C. bezziana.
BIOPROSPEKSI EKSTRAK JAHE GAJAH SEBAGAI ANTI-CRD: KAJIAN AKTIVITAS ANTIBAKTERI TERHADAP MYCOPLASMA GALLISEPTIKUM DAN E. COLI IN VITRO Rahminiwati, Min; P., Aulia Andi Mustika; Saadah, Siti; Andriyanto, .; Soeripto, .; P., Unang
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 1 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

CRD is chronic respiratory disease in chicken caused by infection of Mycoplasma gallisepticum (M gallisepticum) and E. coli. Rio-prospective of jahe for controlling the disease was investigated through the study of antibacterial activity against M. gallisepticum of fresh ginger juice extract and fraction of hexan, ethyl acetate, methanol and water against M. gallisepticum and E. coli. The results showed that the juice of fresh ginger inhibited the growth of M. gallisepticum with the minimum inhibitory concentration that could inhibit the growth was 10 °/o. The fractions that effectively inhibited the growth of M. gal/isepticum are hexan fraction and water fraction with the smallest inhibition zone was found at concentration of at least 8 % and 10 % respectivelly.TLC examination results of hexan fraction showed a purple spot with Rf value of 0.9 and a dark blue spot with Rf value of 0.36. Based on Rf values and color reference, the first spot was suggested zingiberen and the second spot was gingerol. All fractions that were examined, did not show any inhibitory activity against thegrowth of E coli. Thus the extract of fresh ginger was only to be used to control the respiratory disease causedby M. gallisepticum but not coli.
GABUNGAN EKSTRAK RIMPANG TEMULAWAK, DAUN TANJUNG, DAN DAUN BELIMBING MANIS BERDASARKAN ELECTROCARDIOGRAM BERPOTENSI SEBAGAI ANTIARITMIA PADA KUCING Rahminiwati, Min; Safitri, Widia; Noviana, Deni
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.3.409

Abstract

Mimusops elengi L. , Averrhoa carambola L. and Curcuma xanthorrihiza Roxb singly were reported to have hypothensif effect. Mechanism underlying decrease of blood pressure was suggested through modification of cardiac activity. The Effect of Mimusops elengi L., combined with Averrhoa carambola L. and Curcuma xanthorrihiza Roxb extract on cardiac activity were studied using ECG on 12 male cats that were grouped to be a control group administered aquadestilata, and treatment group administered extract of 21 mg and 82 mg/2 kg bw orally respectively. The extracts were given 3 h prior to ECG. The results showed a decrease in P wave, QRS complex and the speed of the heart rate after administration of combination of extract . However QT and PR intervals were increased. This showed that the extract can weaken a contraction of the Atria and ventricles, prolonging the onset of the occurrence of atrial contraction towards ventricular contraction, extending the onset of contraction and relaxation of the ventricles and cause a decrease in heart rate. Based the cardiogram, It was concluded that their combination is valuable for treatment of aritmia.
BIOAKTIVITAS EKSTRAK METANOL DAUN NAMNAM SERTA KOMBINASINYA DENGAN MADU TRIGONA Sumarlin, La Ode; Suprayogi, Agik; Rahminiwati, Min; Tjahja, Achmad; Sukandar, Dede
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 2 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.745 KB) | DOI: 10.6066/jtip.2015.26.2.144

Abstract

Namnam (Cynometra cauliflora L) and trigona honey are two natural ingredients that are potential to be developed in Indonesia. However, scientific evidence of its active compounds and bioactivity is still rarely found, particularly the combination of these two materials. Therefore, this research is to explore their active ingredients of combination of these two materials such as total phenolic, flavonoids, vitamin C and ?-carotene and bioactive capabilities such as antioxidant activity and antibacterial activity. The analysis showed that antibacterial activity of methanol extract of Namnam leaves (EMDN) and trigona honey either in their sole form or combined form has antioxidant and antibacterial activity. The EMDN was more sensitive to Staphylococcus aureus (23.7±3.3 mm) than to Escherichia coli, while the Trigona honey (MT) more sensitive to Escherichia coli (32.6±4.4 mm) than Staphylococcus aureus (16.6±4.1 mm). Similarly, the combination of EMDN and MT was more sensitive to Escherichia coli (23±1.9 mm) than Staphylococcus aureus (17.6±2.6 mm). Analysis of the antioxidant activity also showed that EMDN provided the highest activity (IC50 0.0048±0.000 mg/mL), a combination EMDN and MT (IC50 0.0085±0.000 mg/mL) and MT (IC50 3.736±0.112 mg/mL). Moreover, this analysis also showed thatsole samples of MT and EMDN have total phenolic content, flavonoids, and vitamin C that were higher than the combination of MT and EMDN. However, the content of ?-carotene in the combined form of MT and EMDN was higher. Thus trigona honey, methanol extract of leaves Namnam (Cynometra cauliflora L) in a single form or in a combination are potential to be utilized and developed as a source of antioxidants and antibacterial in the form of functional food.
POTENSI JARAK PAGAR (JATROPHA CURCAS) SEBAGAI LARVASIDA HAYATI PENCEGAH PENYAKIT DEMAM BERDARAH DENGUE Iswantini, Dyah; Riyadhi, Adi; Kesumawati, Upik; Rosman, Rosihan; Mangunwidjaja, Djumali; Rahminiwati, Min
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Recently, Indonesia has big problem caused by dengue haemorraghic fever with A. aegypti as vector. Potential medicine and vaccine for curing this disease have not been found. The effective method to prevent this disease is the use of A. aegypti larvicidal. The chemical larvicidal has disadvantage for evironmental aspect. To solve this problem, the biological larvicidal has a good chance to develop. Indonesia has megadiversity which can be developed as biological larvicidal. Crude aqueous extracts and ethanol extracts of Jatropha curcas (Jarak pagar) seed and oil of Jatropha curcas were evaluated for larvicidal potential against the Aedes aegypti mosquito. Among all extracts, Jatropha curcas oil possessed a highest activity against the 3th instar larvae of Aedes. LC50 values of Jatropha curcas oil was 1507 ppm for 24 h and 866 ppm for 48 h. It was suggested that the Croton tiglium oil and Jatropha curcas oil possess larvicidal properties that could be developed and used as biological larvicidal. 
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN DESTILAT JAHE DAN SIRIH TERHADAP MYCOPLASMA GALLISEPTICUM DAN ESCHERICHIA COLI (ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT AND DESTILAT OF GINGERS AND PIPER BETLES AGAINST MYCOPLASMA GALLISEPTICUM AND ESCHERICHIA CO Rahminiwati, Min; Mustika, Aulia Andi; Zaim, Agung; Sutardi, Lina Novianti
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Betle and ginger juice extracts have been reported to have antibacterial activity against E. coliand M. gallisepticum. However, fractionation analysis showed the have differences in their antibacterialpotency which appeared to be associated with the nature of the solvent polarity. This study wasconducted to obtain information about antibacterial activity of Piper betles and gingers extract againstE. coli and M. gallisepticum. Three types of betle, P. betle, P. betle var nigra, P. crocatum, wereexamined for their antibacterial activity using disc method and three types of ginger, Zingiber officinale,Z. officinale Linn var rubrum, and Z. majus rumph which were extracted by soxhletation using ethanol30%, 60% and 90% as well as distestillation for three and five hours. Piper betle and P. crocatumconsistently have antibacterial effect against E. coli whereas Z. officinale consistently has antibacterialeffect against M. gallisepticum, either extracted by distillation or soxhletation. Piper betle is potentialyield of distillate and extract that has the highest antibacterial activity against E. coli, and M.gallisepticum with inhibitory zone were 9.76 mm and 22 mm respectively.
EVALUASI IN VITRO KEMAMPUAN PENYERAPAN GLUKOSA OLEH EKSTRAK DAUN NAMNAM (CYNOMETRA CAULIFLORA) PADA OTOT DIAFRAGMA TIKUS Sumarlin, La Ode; Suprayogi, Agik; Rahminiwati, Min; Satyaningtijas, Aryani
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 4, No. 1, Mei 2018
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.795 KB) | DOI: 10.15408/jkv.v4i1.7345

Abstract

Diabetes Melitus (DM) adalah kelompok gangguan metabolisme yang ditandai oleh hiperglikemia akibat kerusakan pada sekresi insulin, kerja insulin atau keduanya.  Penderita Diabetes Melitus Tipe 2 (DMT2) mencapai 90-95% dari keseluruhan populasi penderita diabetes tersebut. Salah satu tanaman yang potensial sebagai alternatif obat antidiabetes adalah tanaman namnam (Cynometra cauliflora).  Pada penelitian ini kemampuan penyerapan glukosa oleh otot diafragma yang diisolasi dari tikus non diabetes Sprague Dawley oleh ekstrak metanol daun namnam (C. cauliflora) pada berbagai konsentrasi ditentukan.  Gugus fungsi senyawa dominan pada ekstrak  menggunakan FTIR (Fourier Transform Infrared). Hasilnya menunjukkan bahwa terjadinya peningkatan penyerapan glukosa oleh diafragma ketika konsentrasi ekstrak daun namnam (C. cauliflora) meningkat dan tertinggi pada konsentrasi 450 mg/mL dengan serapan 0.4254 ± 3.23 mg/mL/30 menit dengan peningkatan sebesar 373.72% dibandingkan kontrol air.  Diduga kemampuan ekstrak tanaman C. Cauliflora menyerap glukosa disebabkan keberadaan golongan flavonoid. Oleh karena itu daun tanaman namnam (C. cauliflora) memiliki potensi sebagai obat antidiabetes DMT2 melalui mekanisme peningkatan penyerapan glukosa ke dalam sel.DOI:http://dx.doi.org/10.15408/jkv.v4i1.7345
Dyspepsia in Nonsteroidal Anti-inflammatory Drugs Gastropathy Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 2, August 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) gastropathy is a common complication, which has characteristic symptoms of dyspepsia syndrome. Mostly, it includes epigastric discomfort with bloating and nausea. The aim of this study was to provide evidences that clinical symptoms of dyspepsia are related to macroscopic changes of gastric form in rats, which are expected to be applied in human. Method: The study was conducted in 20 white rats (Rattus norvegicus, Sprague-Dawley strain) at the Department of Pathology and Clinical Reproduction, Bogor Agricultural University between January and December 2008. The rats were divided to treatment group and control group and each group consisted of 10 rats. Acetyl salicylic acid (ASA/aspirin) was administered at 400 mg dose, diluted in distilled water and was given to the treatment group using gastric cannula, once daily for three days period; while the control group had received aquabidest only. Subsequently, necropsies were conducted for both groups, followed by macroscopic observation and measurement of sagittal and transversal diameter. Gastric incisions along the minor curvature were performed in both groups to recognize any macroscopic changes of gastric mucosa. ANOVA test was utilized for data analysis, which was followed by Duncan test when the results were significant. Results: Gastric diameters in treatment group with positive lesion were significantly different from the control group and the treatment group with negative lesion on anthrum/pylorus region, with p < 0.05. Conclusion: Prominent gastric dilatation at anthrum/pylorus region found in the treatment group may become the initial cause and signs of dyspepsia in human. Keywords: NSAID gastropathy, dyspepsia, NSAIDs/aspirin, gastric dilatation
Preventing NSAID-induced Gastropathy: The Role of Mucus Cells to Prevent Aspirin-Induced Acute Gastric Mucosal Damage Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 3, December 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Mucus is pre-epithelial gastric layer that may prevent damages due to direct contact between aspirin and gastric epithelial cells. The integrity of gastric mucosa and mucous cellular reaction may serve as primary and secondary prevention of extended aspirin-induced gastric mucosal damage. The aim of this study was to prove the function of mucus as defensive factor in rats. Method: The study was conducted in twenty white rats of the Sprague-Dawley strain at Department of Pathology and Clinical Reproduction, Bogor Agricultural University, between January and December 2008. The rat in the treatment group were given 400 mg aspirin diluted in aqua bidest through intra- gatric canules; while the control group received aqua bidest only once daily for 3 days. Necropsies, macroscopic and microscopic observation were performed by counting the number of Alcian blue- periodic acid Schiff-stained mucous cells at fundus/corpus and antrum/pylorus regions. Data analysis was performed using ANOVA and Duncan test. Results: The number of mucous cells with positive lesions in the treatment group was significantly different from the control group at both regions. There was no significant difference of negative lesions between treatment and control group at both regions. At antrum/pylorus region, there was no difference of negative lesions between treatment and control groups; however, both groups demonstrated significant difference of positive lesions in treatment group. Conclusion: In primary prevention for gastric mucosal lesions, there is no increasing number of mucous cells in normal mucosa. Increasing number of mucous cells is a secondary prevention against extended aspirin-induced gastric mucosal damage. Keywords: NSAIDs/ASA, mucus cells, gastric mucosal lesion, rat
LIPASE ACTIVITY OF ENDOPHYTIC ACTINOBACTERIA FROM MEDICINAL PLANTS Lestari, Yulin; Wirawan, Budi; Budiarti, Sri; Rahminiwati, Min
HAYATI Journal of Biosciences Vol. 25 No. 1 (2018): January 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.91 KB) | DOI: 10.4308/hjb.25.1.1

Abstract

Endophytic bacteria are known to reside within host plant tissue without giving a harmfull effect. The endophytes may play an important role, as they may produce similar bioactive compounds as produced by the host plant. Various medicinal plants have long been used to cure diseases. Traditionally, leaves extract of Guazuma ulmifolia, Psidium guajava, or the rhizome of Curcuma xanthorrhiza can be used to treat disease, e.g. hyperlipidemic. The mechanism can be through lipase activity, where the lipase catalyzes the hydrolysis of triacylglycerol to fatty acids and acylglycerol. The objective of this research was to assess potency of endophytic bacteria as anti-hyperlipidemic compounds producer through their lipase activity. Sixty nine endophytic bacteria which comprised of 22, 27 and 20 isolates were isolated from the leaves of G. ulmifolia, P. guajava, and the rhizome of C. xanthorrhiza, respectively. Eight out of the 69 isolates showed lipase activity, and the two selected isolates, i.e. DPG 3(2) and AJB 4(4) were considered as good lipase producers. The highest specific lipase activity of DPG 3(2) isolate was observed for 0.874 units per mg at 38 h, whereas AJB 4(4) isolates had the specific lipase activity at 1.139 units per mg after 72 h observation. These data indicate that the two selected isolates have the potency as antihyperlipidemic compounds producer through their lipase activity.