Articles

Found 13 Documents
Search

ABERASI KROMOSOM PADA PILOT DAN AWAK KABIN MASKAP AI PENERBANGAN KOMERSIAL Ramadhani, Dwi; Purnami, Sofiati; Suvifan, Viria Agesti
Buletin Alara Vol 13, No 2: Desember 2011
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2351.169 KB)

Abstract

Tidak ada abstrak
AUTOMATED ESTIMATION PARASITEMIA OF PLASMODIUM BERGHEI INFECTED MICE USING CELLPROFILER SOFTWARE Ramadhani, Dwi; Nurhayati, Siti
INKOM Journal Vol 7, No 2 (2013)
Publisher : Pusat Penelitian Informatika - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/j.inkom.263

Abstract

In this paper, we propose a technique for automatically recording parasitemia of mice infected with Plasmodium berghei by using Cellprofiler. Our purpose is to identify the difference number of parasitemia obtained by CellProfiler and manual assessment. We conducted a T-test analysis with p < 0.05. This value is considered to have a statistically significant different between automatic and manual process. Total of 50 thin blood smear images were analyzed for both automatically using CellProfiler and manual process. Results showed that there were insignificant difference between automatic and manual process (p > 0.05). It can be concluded that based on this research that automated quantification of parasitemia using CellProfiler was comparable but not better than manual.keywords: Automation, CellProfiler, Parasitemia, Plasmodium berghei, Thin blood smearPada tulisan ini, penghitungan parasitemia pada mencit yang diinfeksi dengan Plasmodium berghei dengan CellProfiler diusulkan. Tujuan penelitian yang dilakukan adalah untuk mengetahui apakah terdapat perbedaan antara nilai parasitemia yang diperoleh dengan menggunakan CellProfiler dibandingkan dengan secara manual. Uji T digunakan untuk analisis statistik dengan p < 0, 05 berarti terdapat perbedaan yang signifikan antara penghitungan otomatis dibandingkan dengan manual. Total sebanyak lima puluh citra apusan darah tipis mencit dianalisis secara otomatis menggunakan CellProfiler dan secara manual. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan signifikan antara nilai persentase yang diperoleh secara otomatis dibandingkan dengan manual (p > 0, 05) . Dengan demikian dapat disimpulkan bahwa nilai persentase parasitemia yang diperoleh secara otomatis menggunakan CellProfiler sebanding tetapi tidak lebih baik dibandingkan dengan teknik manual.kata kunci: CellProfiler, Otomatisasi, Parasitemia, Plasmodium berghei, Preparat Apusan Tipis
PEMAHAMAN KONSEP MATEMATIS SISWA PADA MATERI KESEBANGUNAN MELALUI PEMBELAJARAN COOPERATIVE TIPE NUMBERED HEADS DISERTAI LKS Ramadhani, Dwi; Haryono, Yulia; Lovia, Lita
Pendidikan Matematika Vol 2, No 2 (2013): Jurnal Wisuda Ke 47, Genap 2013-2014 Pendidikan Matematika
Publisher : STKIP PGRI Sumbar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research is introduced by the lack of the students understanding of mathematical concept and students prefer to copy paste their friends exercise without paying attention it is right or wrong. This research is aimed to know the students understanding of mathematicalconcept by using learning model of Cooperative type Numbered Heads with LKS better than the students understanding concept by using conventional learning at class IX SMP PERTI Padang. The type of this research is experiment. Population in this research is students class IX SMP PERTI Padang who registered in academic year 2013/2014 that consists of three class. The tecgnique of sampling is random and selected class IX.1 as an experimental class and class IX.2 as control class. Insrument used was final test contain mathematical concept umderstanding indicators in the from of essay. Technique of data analysis used was Mann-Withney test with MINITAB sofware. Based on the hypothesis obtained P-value = 0,003, because p-less than α = 0,05 so the hypotesisis accepted the students understanding of mathematical concept by using Cooperative type Numbered Heads learning with LKS is better than students understanding mathematical concept by using conventional learning at class IX SMP PERTI Padang.
Analisa Efektifitas Fasilitas Zebra Cross Pada Jl. MT Haryono dan Jl. Gajayana Arifin, M. Zainul; Prawito, Gagoek Soenar; Ramadhani, Dwi
Rekayasa Sipil Vol 1, No 1 (2007)
Publisher : Department of Civil Engineering, Faculty of Engineering, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.062 KB)

Abstract

Kemajuan Kota Malang yang semakin pesat kurang diimbangi dengan fasilitas jalan yang memadai terutama untuk pejalan kaki. Berdasarkan data kepolisian, rata-rata kecelakaan di JL. MT. Haryono dan Jl. Gajayana Malang melibatkan pengendara kendaraan bermotor dengan penyeberang jalan. Penyeberang jalan kurang memanfaatkan fasilitas zebra cross untuk menyeberang. Begitu pula dengan pengendara kendaraan bermotor, mereka cenderung kurang mengutamakan penyeberang jalan ketika akan menyeberang, kecepatan mereka relatif konstan. Maka dari itu diadakan penelitian untuk mengetahui efektifitas dari zebra cross apakah keberadaannnya sudah benar-benar tepat, sehingga dapat diambil sebagai dasar pengambilan tindakan dalam mengatasi masalah kecelakaan lalu lintas. Tujuan penelitian ini adalah untuk mengetahui efektifitas fasilitas zebra cross dengan parameter penurunan kecepatan kendaraan pada saat akan melewati zebra cross.Dari hasil perhitungan statistik, pada periode waktu pertama didapatkan kecepatan kendaraan pada jarak 30 m adalah 38,63150 km/jam dan pada jarak 50 m adalah 37,00380 km/jam. Sedangkan kecepatan kendaraan pada periode waktu kedua pada jarak 30 m adalah 37,3452 km/jam dan pada jarak 50 m adalah 35,5920 km/jam. Hal ini menunjukkan bahwa pada periode waktu pertama dan kedua, kecepatan kendaraan pada jarak 30 m lebih besar dibandingkan dengan kecepatan pada jarak 50 m. Jadi kesimpulannya zebra cross di Jl. MT Haryono dan Jl. Gajayana tidak efektif, berdasarkan tidak adanya penurunan kecepatan kendaraan pada saat akan melewati zebra cross. Kecepatan kendaraan cenderung naik pada saat akan melewati zebra cross. 
EVALUATION OF PERIPHERAL BLOOD LYMPHOCYTES PROLIFERATION IN BOTTENG VILLAGE, MAMUJU INHABITANTS USING BINUCLEATE INDEX Ramadhani, Dwi; Sardini, Sri; Lubis, Masnelli; Syaifudin, Mukh
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 12, No 2 (2016): Desember 2016
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.592 KB) | DOI: 10.17146/jair.2016.12.2.3564

Abstract

Botteng Village in Mamuju, West Sulawesi was known for the high natural background radiation exposure. Botteng Village inhabitants exposed to high natural radiation in their daily life. Radiation exposure can inhibit the mitosis mechanism at various phases. Our previous study revealed that mitotic and nuclear division indexes in Botteng Village inhabitants were lower compared to control samples. To validate our previous study results here we evaluate the binucleate index in peripheral blood lymphocytes of Botteng Village inhabitants. Blood samples were collected from thirteen healthy adult subjects in Botteng Village and thirteen healthy adult subjects in normal background radiation area. Binucleate index was calculated as the proportion of binucleated cell (BNC) in 500 cells for each sample. Our study showed that the BI in Botteng Village was higher compared to control group (23.58 ± 9.60 vs 23.47 ± 6.24). Statistical analysis revealed that the different was not significant (p=0.973). It is possible that the small sample numbers used in this study were not adequate to represent the BI value in Botteng Village inhabitants. This study also showed that there was insignificant difference of BI in respect to gender and age for all samples. Further study using larger sample number should be conducted to ensure the possibility of BI to evaluate the effect of chronic low radiation dose exposure on lymphocytes proliferation.
FREQUENCIES OF MICRONUCLEUS IN MONONUCLEATED CELLS OF PEOPLE LIVING IN TAKANDEANG VILLAGE – A HIGH LEVEL OF NATURAL RADIATION AREA IN INDONESIA Purnami, Sofiati; Lubis, Masnelli; Suryadi, Suryadi; Nurhayati, Siti; Ramadhani, Dwi; Syaifudin, Mukh
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 14, No 2 (2018): Desember 2018
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.156 KB) | DOI: 10.17146/jair.2018.14.2.4624

Abstract

Our previous study showed that the micronucleus frequencies of Botteng Village inhabitants in Mamuju (a high background radiation area) were higher compared to control samples even though the difference was not significant. To validate our previous study result, here in this study we assessed the micronucleus frequencies in mononucleated cells of Takandeang Village inhabitants. Twenty seven healthy adult subjects from Takandeang Village and normal background radiation area were included in this study. Micronucleus numbers (MN) in 1000 mononucleated cells (MNC) were evaluated in this study. Results showed that mean MN numbers in MNC (MNMNC) of Takandeang Village inhabitants were significantly higher compared to control samples [3.96 ± 0.488 vs 1.96 ± 1.675; p<0.05]. The age and gender factors did not affect the MNMNC in all samples (p>0.05). It is possible that the high background radiation exposure received by Takandeang Village inhabitants induced not only the clastogenic but also aneugenic effect in lymphocytes. Overall our study showed that scoring of MNMNC can provide additional information in cytokinesis block micronucleus (CBMN) assay. Further study should be performed to validate this study results. The 24 hours culture time should be used in the further study to obtain more comprehensive results. 
HAEMOZOIN DETECTION IN MOUSE LIVER HISTOLOGY USING SIMPLE POLARIZED LIGHT MICROSCOPE RAMADHANI, DWI; NURHAYATI, SITI; RAHARDJO, TUR
HAYATI Journal of Biosciences Vol. 21 No. 1 (2014): March 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (877.9 KB) | DOI: 10.4308/hjb.21.1.48-52

Abstract

The presence of malarial pigment (haemozoin) due to Plasmodium infection is a common histopathological effect in mouse liver. Previous research showed that by using a polarized light microscope, researchers were better able to detect haemozoin in mouse liver histology section. Thus, the aim of this research was to compare the haemozoin area observed by a conventional vs. simple polarized light microscope by using image processing analysis. A total of 40 images produced from both conventional light microscope and simple polarized light microscope were collected. All images were analyzed using ImageJ 1.47 software to measure the haemozoin areas. Our results showed that non birefringent haemozoin and birefringent haemozoin area was significantly different. This was because when using conventional light microscope the brown area that contained images of non birefringent haemozoin images also contained Kupffer cells which appeared as the same brown color as haemozoin. In contrast, haemozoin gave bright effect and can be easily differentiated with Kupffer cells in the birefringent haemozoin images. This study concluded that haemozoin detection in mouse liver histology using a simple polarized light microscope was more accurate compared to that of conventional light microscope.
AUTOMATED DETECTION OF BINUCLEATED CELL AND MICRONUCLEI USING CELLPROFILER 2.0 SOFTWARE RAMADHANI, DWI; PURNAMI, SOFIATI
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.964 KB) | DOI: 10.4308/hjb.20.4.151-156

Abstract

Micronucleus assay in human peripheral lymphocytes usually used to assess chromosomal damage. Manual scoring of micronuclei can be time consuming and large numbers of binucleated cells have to be analyzed to obtain statistically relevant data. Automation of the micronuclei analysis using image processing analysis software can provide a faster and more reliable analysis of micronucleus assay. Here the used of CellProfiler an open access cell image analysis software for automatic detection of binucleated cells and micronuclei were reported. We aimed to know whether there was a significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. Wilcoxon Rank test was used for statistical analysis to test H0 hypothesis that there was no significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. We analyzed 135 images for both manual and CellProfiler counting. Our results showed that there was no significant difference between manual and CellProfiler counting for binucleated cells (P = 0.851) and for micronuclei (P = 0.917). In conclusion, the binucleated cells and micronuclei counting using CellProfiler were comparable but not better than manual counting.
LIVER HISTOPHATOLOGICAL STUDIES OF MICE (MUS MUSCULUS SP.) INFECTED WITH GAMMA RAYS IRRADIATED PLASMODIUM BERGHEI STRAINS ANKA RAHARDJO, TUR; NURHAYATI, SITI; RAMADHANI, DWI
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.115 KB) | DOI: 10.4308/hjb.20.3.144

Abstract

Malaria is a serious global disease and a leading cause of morbidity and mortality in tropical and sub-tropical countries. Radiation attenuated vaccines have been demonstrated to be an effective means of controlling certain parasitic infections such as malaria. Our previous research showed that irradiation dose of 150-175 Gy to Plasmodium berghei were enable to suppress the infected erythrocyte cells and irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on parasitemia index. Aim of this research was to investigate the most suitable irradiation dose to attenuated P. berghei based on the the histological changes in the liver mouse malaria model. Histological changes in mice liver because of Plasmodium is congestion in the sinusoids and masses of hemozoin. Fourty Swiss mice were randomly assigned to four equal groups. First group of mice were inoculated with the unirradiated malaria parasites as a positive control while negative control group represented uninfected normal control. The third group of mice was inoculated with the irradiated malaria parasites with dose 175 Gy. Last group were inoculated with the irradiated malaria parasites with dose 150 Gy. Mice were sacrificed at day 6, 8, 12, 16, 20, and 24 post inoculation for liver histological study. Their livers processed for histomorphometric assessment of sinusoidal area, haemozoin area and hepatosomatic index as a quantitative measure of altered morphology. Hepatic sinusoidal area in mice infected with the unirradiated malaria parasites increased by 104% in day 24 post inoculated compared to day 6 post inoculated, whereas a smaller increase of 46% occurred in mice that were inoculated with the irradiated malaria parasites with dose 150 Gy. The difference between positive control and 150 Gy group was significant (P < 0.05).  The highest average haemozoin area in all days post inoculation was in positive control whereas a smaller occurred in mice that were inoculated with the irradiated malaria parasites with dose 175 Gy, but the different was not significant (P > 0.05). For hepatosomatic index (HSI) at day 24 post inoculation the HSI value in group 150 Gy (5.33) was the smallest compared to other groups. Based on our research it can be concluded that irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on assessment of sinusoidal area, and HSI value at 24 day post inoculation.
Deteksi Sel Rogue Pada Sel Limfosit Darah Tepi Pasien Kanker Serviks Pra dan Paska Kemoradioterapi Ramadhani, Dwi; Soetopo, Setiawan; Kurjana, Tjahya; S Hernowo, Bethy; DL Tobing, Maringan; Tetriana, Devita; Suvifan, Viria Agesti; Purnami, Sofiati; Lusiyanti, Yanti
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 1 (2016): Juni 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.755 KB)

Abstract

Proses penentuan nilai dosis radiasi pengion berdasarkan indikator biologis atau biodosimetri umumnya dilakukan berdasarkan analisis kromosom disentrik. Proses biodosimetri berdasarkan analisis kromosom disentrik yang tidak boleh melibatkan kromosom disentrik dalam sel rogue karena nilai dosis yang diperoleh lebih tinggi dari nilai sebenarnya. Sel rogue adalah sel dalam tahap metafase yang berasal dari kultur sel limfosit darah tepi dan memiliki jumlah aberasi kromosom sangat tinggi meskipun sampel darah tidak terpapar oleh radiasi pengion. Hingga kini belum diketahui secara pasti penyebab timbulnya sel rogue dalam sel limfosit darah tepi. Terdapat Didugaan bahwa infeksi virus atau bakteri penyebab terbentuknya sel rogue. Dugaan lainnya menyatakan bahwa paparan radiasi dengan Linear Energy Transfer (LET) tinggi adalah penyebab timbulnya sel rogue. Tujuan penelitian adalah mendeteksi keberadaan sel rogue pada pasien kanker serviks sebelum dan sesudah dilakukan proses kemoradioterapi. Prediksi nilai dosis radioterapi dilakukan berdasarkan jumlah kromosom disentrik dengan atau tanpa melibatkan kromosom disentrik dalam sel rogue. Sebanyak 20 ml sampel limfosit darah tepi dari lima pasien kanker serviks paska radioterapi dikultur, dibuat preparatnya dan diamati. Hasil penelitian menunjukkan adanya satu sel rogue pada salah satu pasien kanker serviks paska kemoradioterap. yang diakibatkan oleh paparan radiasi. Prediksi dosis menunjukkan bahwa nilai prediksi dosis dengan melibatkan kromosom disentrik dalam sel rogue menyebabkan nilai dosis yang diperoleh lebih tinggi dari nilai sebenarnya. Berdasarkan hasil penelitian yang dilakukan dapat disimpulkan bahwa paparan radiasi pengion dapat mengakibatkan terbentuknya sel rogue pada individu dengan tingkat radiosensitivitas tinggi. Dengan demikian proses biodosimetri berdasarkan analisis kromosom disentrik tidak dapat dilakukan dengan melibatkan kromosom disentrik dalam sel rogue. The process of determining the ionizing radiation dose based on biological indicator or biodosimetry is generally carried out using the analysis of dicentric chromosome. Biodosimetry process based on the analysis of dicentric chromosome should not involving the dicentric in a rogue cell that may cause the radiation prediction doses value more higher than the true doses value. Rogue cells is cells in metaphase derived from the peripheral blood lymphocytes culture and contain a high number of chromosome aberration even though the blood sample were not exposed to ionizing radiation. Until now it was not clear what factor that can induce the rogue cells in peripheral blood lymphocytes. There was suggestion that infection of virus or bacteria and radiation exposure of high linear energy transfer (LET) can induce rogue cells. Aim of this research was to detect the presence of rogue cells in cervical cancer patients before and after radio chemotherapy process. The prediction of radiotherapy doses was carried out with and without involving the dicentric chromosomes in the rogue cells. Twenty milliliter of blood samples from five cervical cancer patients obtained before and after radiotherapy was cultured, harvested and analyzed. The experimental result showed that there was a presence of one rogue cell in one cervical cancer patient after radio chemotherapy process. A radiotherapy prediction doses showed that predictive dose value dose involving dicentric chromosomes in rogue cell was higher compared to the real radiation dose value. Based on the research result it can be concluded that exposure to ionizing radiation can induced the presence of the rogue cells in high radiosensitivity person. It means that in the biodosimetry process based on the analysis of dicentric chromosome should not involve the dicentric chromosome in the rogue cell.