FEDIK ABDUL RANTAM
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

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DETERMINAN ANTIGEN GEN OMP2A BRUCELLA ABORTUS ISOLAT LOKAL Ratnasari, Ratih; Handijatno, Didik; Suwarno, .; Rantam, Fedik Abdul
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.347 KB) | DOI: 10.29244/avi.2.1.17-25

Abstract

Penyakit Brucellosis pada sapi disebabkan oleh Brucella abortus dan dikenal sebagai penyakitreproduksi menular pada ternak. Brucellosis merupakan penyakit zoonosis karena dapat menularpada manusia. Penelitian ini bertujuan untuk mengetahui determinan antigen yang terdapat pada genomp2a B. abortus isolat lokal yang telah diblasting ke asam amino (protein). Sampel bakteri berasaldari sapi penderita Brucellosis asal Sulawesi Selatan dan Nusa Tenggara Timur. Gen omp2a diamplifikasimelalui tehnik Polymerase Chain Reaction (PCR) dengan menggunakan primer 2ab5F dan 2a900R.Produk PCR disekuensing untuk mendapatkan sekuen nukleotida gen omp2a B. abortus isolat lokal.Sekuen nukleotida ini dianalisis tingkat homologinya terhadap isolat asal mancanegara yang diaksesdari GenBank dengan menggunakan BLAST. Sekuen nukleotida gen omp2a diblasting ke asam aminokemudian dengan metode Kolaskar dan Tongaonkar antigenicity dapat diperoleh determinan antigenpada antigen protein membran luar (OMP) B. abortus isolat lokal. Hasil menunjukkan bahwa tingkathomologi antara sekuen gen omp2a B. abortus isolat lokal dengan isolat asal mancanegara mempunyaitingkat homologi yang tinggi (99% - 100%). Hasil prediksi determinan antigen didapatkan enamdeterminan antigen pada antigen OMP B. abortus isolat lokal.
UTILIZATION OF VIABLE BONE MARROW DERIVAT STEM CELLS THROUGH AN ADAPTION IN LOW OXIGEN TENSION AS AN ATTEMPT TO INCREASE CELLULAR TRANSPLANTATION EFFICACY FOR SPERMATOGENESIS PROCESS Safitri, Erma; Utama, Suzanita; Bumi, Candra; Mardi, Sri Wigati; Mulyani, .; Helen, .; Purwati, .; Prasetyo, R. Heru; Hariadi, Mas?ud; Rantam, Fedik Abdul
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Cellular transplantation using stem cells provides very promising solutions in the regeneration and repairment of cells that have experienced degeneration where recovery through medical or surgical intervension is impossible. However, the very low viability of transplanted stem cells limits the transplantation efficacy. The aim of this research was to obtain viable bone marrow derived stem cells by an adaptation treatment in a low oxygen tensioned in vitro culture. Low oxygen tension adaptation was adjusted to the niche of the stem cells in vivo. In this study, in vitro culture of stem cells in 1% oxygen was compared to those of the conventional culture in 21 % oxygen.Results showed that under 1% oxygentension cell proliferation was slower with larger or rounded triangle shaped cells, and senescence or dead cells was low. Meanwhile under 21 % oxygen tension cell proliferation was two fold faster with flattened and slender cells, and senescence or dead cells was higher. In conclusion, conventional in vitro culture under 21% oxygen caused cell aging (senescence) and rapid cell death, therefore the transplanted cells were not viable.
PEMBERIAN BUAH MERAH (PANDANUS CONOIDEUS LAM) SEBELUM DIPAPAR TIMAH HITAM MENEKAN EKSPRESI CASPASE-8 DAN JUMLAH SEL HOFBAUER MENCIT (MUS MUSCULUS) BUNTING (THE PROVISION OF RED FRUIT (PANDANUS CONOIDEUS LAM) BEFORE EXPOSED BY LEAD DECREASE EXPRESSION OF C Wahyuni, Ika; Widjiati, Widjiati; Madyawati, Sri Pantja; Rantam, Fedik Abdul
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.1.128

Abstract

The research aims to observed the provision of red fruit in placenta pregnat mice before exposed by lead. The observed case are expression of caspase-8 and number of hofbauer cells. Red fruit was expected to decrease expression of caspase-8 and number of hofbauer cells. The study design used was complete randomized design. Each treatment utilized four pregnant mice as negative control group (K-), for this group given distilled water orally during gestation 6th-15th. Positive control group (K+) given lead (0.011mg/20 g BW in 1 mL distilled water) orally during gestation 6th-15th. The treatment group namely P1, P2, and P3 treated by red fruit with different doses i.e., 8.29 mg/20 g BW, 23.98 mg/20 g BW, and 25.68 mg/20 g BW, respectively, during gestation 6th-15th, then one hour later exposed with lead. The obtained data were analyzed by Kruskal-Wallis and Mann Whitney test for calculate caspase-8 expression, ANOVA and Duncan for calculate the number of hofbauer cells. The result indicated that treatment groups which provided by red fruit of 0.8 mL/20 g BW and 0.9 mL/20 g BW showed decline expression of caspase-8 and number of hofbauer cells compare control group without admission of red fruit antioxidant and P1. Inconclusion red fruit can decrease expression of caspase-8 and number of hofbauer cell that means decrease apoptosis. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian buah merah pada plasenta mencit bunting sebelum dipapar timah hitam. Variabel yang diamati adalah ekspresi caspase-8 dan jumlah sel hofbauer. Buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer. Penelitian ini menggunakan rancangan acak lengkap (RAL). Setiap kelompok perlakuan menggunakan empat mencit bunting sebagai kelompok kontrol negatif (K-), untuk kelompok ini diberi aquades pada kebuntingan hari ke-6 sampai 15. Kelompok kontrol positif (K+) diberi timah hitam dosis 0,011 mg/20 g BB dalam 1 mL aquades selama kebuntingan hari ke-6 sampai 15. Kelompok perlakuan (P1, P2, dan P3) diberi minyak buah merah dengan dosis yang berbeda yaitu 8,29 mg/20 g BB, 23,98 mg/20 g BB, dan 25,68 mg/20 g BB. Data dianalisis dengan Kruskall-Wallis dan Mann Whitney untuk menghitung ekspresi caspase-8, sidik ragam dan uji Duncan digunakan untuk menghitung jumlah sel hofbauer. Hasil penelitian menunjukkan kelompok perlakuan yang diberi buah merah 23,98 mg/20 g BB dan 25,68 mg/20 g BB dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer dibandingkan dengan kelompok kontrol positif dan P1. Simpulan penelitian ini adalah bahwa minyak buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer yang berarti menekan terjadinya apoptosis.
Efek ekstrak buah delima (Punica Granatum L) terhadap ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster Hernawati, Sri; Rantam, Fedik Abdul; Sudiana, I Ketut; Rahayu, Retno Pudji
Dental Journal (Majalah Kedokteran Gigi) Vol 46, No 3 (2013): (September 2013)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.509 KB) | DOI: 10.20473/j.djmkg.v46.i3.p148-151

Abstract

Background: Squamous cell carcinoma is the most common cancer in the oral cavity. DNA tests showed that almost 90% of cases revealed wild p53 gene mutations. Wild p53 gene mutations cause p53 inactivation so the cell cycle does not stop in G1 phase but continues to S phase and G2 and M, it makes the mutated DNA remains multiplied and apoptosis does not occur. One candidate of the cancer treatment alternatives is pomegranate extract (Punica granatum L – PGL). Purpose: The purpose of study was to examine the effect of PGL on wild p53 expression in oral cavity malignant cell of swiss webster strain mice. Methods: Thirty- two swiss webster strain mice (Balb/c) 5 months old were randomly divided into four groups. Two control groups (K0: no benzopirene exposed and untreated; K1: benzopirene exposed and untreated); and 2 treatment groups (P1: benzopirene exposed and given EA; P2: benzopirene exposed and given PGL extract). The expression of wild p53 was determined by immunohistochemical techniques. Results: The results showed that administration of PGL could increase the expression of wild p53 in malignant epithelial cells in the oral mucosa of mice, and the expression was higher than EA. Conclusion: This study suggested that the PGL extract could express wild p53 in the oral cavity malignant cells of swiss Webster strains mice.Latar belakang: Karsinoma sel skuamosa merupakan kanker yang sering terjadi pada rongga mulut. Pemeriksaan DNA menunjukkan hampir 90% kasus dijumpai adanya mutasi gen wild p53. Mutasi gen wild p53 menyebabkan inaktivasi wild p53 sehingga siklus sel tidak berhenti pada fase G1 tetapi berlanjut ke fase S dan G2 dan M, sehingga DNA yang mengalami mutasi tetap dilipatgandakan dan apoptosis tidak terjadi. Salah satu kandidat obat kanker adalah ekstrak buah delima (Punica Granatum L - PGL). Tujuan: Penelitian ini bertujuan untuk meneliti efek ekstrak PGL terhadap ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster. Metode: Tiga puluh dua ekor mencit (Balb/c) strain swiss webster jantan berumur 5 bulan dibagi secara random menjadi 4 kelompok, yaitu 2 kelompok kontrol (K0: tidak dipapar benzopirene dan tidak diberi perlakuan; K1: dipapar benzopirene dan tidak diberi perlakuan); serta 2 kelompok perlakuan (P1: dipapar benzopirene dan diberi EA; P2: dipapar benzopirene dan diberi ekstrak PGL). Pemeriksaan ekspresi wild p53 dilakukan dengan teknik  imunohistokimia. Hasil: Hasil penelitian menunjukkan bahwa pemberian ekstrak PGL dapat meningkatkan ekspresi wild p53 pada sel epitel ganas pada mukosa rongga mulut mencit, dan lebih tinggi dibanding dengan pemberian EA. Simpulan: Penelitian ini menunjukkan bahwa ekstrak PGL dapat meningkatkan ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster
CHARACTERIzATION of CD4 + T LYMPHOCYTE FROM BONE MAROW STEM CELL USING INDIRECT IMMUNOFLUORESENCE FOR HIV & AIDS TREATMENT Purwati, Purwati; Nasronudin, Nasronudin; Rantam, Fedik Abdul
Indonesian Journal of Tropical and Infectious Disease Vol 1, No 3 (2010)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2298.797 KB) | DOI: 10.20473/ijtid.v1i3.2195

Abstract

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HIV). At the beginning of infection, gp120 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gp120 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell. Therefore, CCR5 and CXCR4 also determine the fate of the target cell. It is the performance of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HIV infection. Coding gene CCR5 may mutate to become protective or resistant against HIV infection. In homozygote individuals, it tends to be resistant against infection, while in heterozygote individuals it tends to be susceptible to HIV infection. Objective: To characterize TCD4 lymphocyte in the next that is resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HIV and comparison to control. Result: This study was performed in several steps, such as mononucleated cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification. Conclusion: Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment.
ANTIBODY OF GOAT ZONA PELLUCIDA-3 (GZP3) PROTEIN OF MICE(MUS MUSCULUS) BLOCK IN VITRO FERTILIZATION OF MICE AS AN ANIMAL MODEL= ANTIBODI PROTEIN ZONA PELUSIDA-3 KAMBING (GZP3) ASAL MENCIT(MUS MUSCULUS) MENCEGAH ... Mustofa, Imam; Mahaputra, Laba; Dachlan, Yoes Priyatna; Rantam, Fedik Abdul; ., Suwarno; ., Widjiati; Hinting, Aucky
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2056.388 KB) | DOI: 10.22146/jsv.344

Abstract

The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (p
PENENTUAN PROTEIN ANTIGEN LIMFOSIT SAPI YANG BERASAL DARI SAPI BALI DAN SAPI PERANAKAN ONGOLE (DETERMINATION OF THE PROTEIN OF BOVINE LYMPHOCYTE ANTIGEN, OF BALI CATTLE AND ONGOLE CROSS BREED CATTLE) Suwiti, Ni Ketut; Rantam, Fedik Abdul
Jurnal Veteriner Vol 4 No 2 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

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Ekspresi protein P53 dan HSP70 pada sel punca karsinoma nasofaring yang resisten terhadap radioterapi Yusuf, Muhtarum; Romdhoni, Ahmad Chusnu; Kentjono, Widodo Ario; Rantam, Fedik Abdul
Oto Rhino Laryngologica Indonesiana Vol 44, No 2 (2014): Volume 44, No. 2 July - December 2014
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.659 KB) | DOI: 10.32637/orli.v44i2.93

Abstract

Latar belakang: Pada penderita Karsinoma Nasofaring (KNF) masih sering ditemukan kekambuhan meskipun sudah mendapat terapi yang lengkap. Penelitian terbaru membuktikan bahwa kekambuhan disebabkan oleh sel punca KNF yang resisten terhadap radioterapi. Mekanisme resistensi sel punca kanker terhadap radioterapi diduga karena hambatan terhadap apoptosis dan atau memicu proliferasi. Hambatan terhadap apoptosis disebabkan oleh penurunan protein p53 (wild type), selain over-ekspresiHsp70. Tujuan: Menjelaskan mekanisme resistensi sel punca KNF terhadap radioterapi berdasarkan profil ekspresi protein p53(wild type)dan Hsp70. Metode: Penelitian true experimental dengan menggunakan rancangan randomisasi kelompok kontrol sebelum dan sesudah tes. Kultur sel punca KNF dibagi menjadi dua kelompok, masing-masing 16 sampel. Pada kelompok perlakuan diberikan paparan radioterapi dengan dosis 1,5 Gy menggunakan pesawat Linac, lalu diinkubasi selama 24 jam. Sebelum dan sesudah perlakuan pada kedua kelompok diperiksa ekspresi p53 (wild type) dan Hsp70. Pemeriksaan menggunakan metode flowcytometry. Hasil: Ekspresi p53 (wild type) antara kelompok perlakuan dan kontrol terdapatperbedaan yang tidak bermakna dengan p=0,576 (p≥0,05). Ekspresi Hsp70, antara kelompok perlakuan dan kontrol terdapat perbedaan yang tidak bermakna dengan p=0,172 (p≥0,05). Kesimpulan: Tidak terdapatperubahan ekspresi p53 (wild type) dan Hsp70 pada sel punca KNF yang resisten terhadap radioterapi.Kata kunci : Sel punca KNF, p53 (wild type), Hsp70, karsinoma nasofaring ABSTRACTBackground: Recurrences are frequently occurred in nasopharyngeal  carcinoma (NPC) patients, eventhough they had received complete therapy. Recent studies have proved that those recurrences were caused by NPC cancer stem cells that resistant to radiotherapy. Mechanisms of resistance of cancer stem cells to radiotherapy is assumed due to the block of apoptosis and or proliferation inducing. The block of apoptosis was caused by the decrease of p53 (wild type) expression, in addition to Hsp70 over expression. Objective: To find out the mechanism of NPC stem cells that resistant to radiotherapy based on profiles of protein p53 (wild type) and Hsp70 expression. Methods: Using true experimental study by randomizedpre and post test control group design. The cultured NPC stem cells were divided into two groups, with 16 samples each. The treatment group had 1,5Gy dose of radiotherapy exposure with Linac device, then incubated for 24 hours. Both groups were examined for p53 (wild type) and Hsp70 expressions before and after treatment. The examinations were conducted by flowcytometry method. Result: The P53 (wild type) expression between the treatment and control group showed insignificant difference with p=0.576(p≥0.05). The Hsp70 expression between treatment and control group showed insignificant difference with p=0.172 (p≥0.05). Conclusion: There were no changes of p53 (wild type) and Hsp70 expressions on NPC stem cells that resistant to radiotherapyKeywords: NPC stem cells, p53 (wild type), Hsp70, nasopharyngeal carcinoma
Cytotoxicity test and characteristics of demineralized dentin matrix scaffolds in adipose-derived mesenchymal stem cells of rats Sari, Desi Sandra; Maduratna, Ernie; Ferdiansyah, F.; Sudiana, I Ketut; Rantam, Fedik Abdul
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 4 (2018): (December 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1443.079 KB) | DOI: 10.20473/j.djmkg.v51.i4.p194-199

Abstract

Background: Demineralized dentin matrix (DDM) scaffold is a substitute material for the bone contained in human teeth. DDM is a scaffold-derived tooth dentine containing type I collagen and bone morphogenetic protein (BMP). While DDM possesses the ability to perform osteoinductive and osteoconductive roles, a cytotoxicity test of DDM scaffold remains extremely important in evaluating the level of toxicity of a material if cultured in cells. Adipose-derived mesenchymal stem cells (ADMSCs) are multipotent in nature because they contain progenitor cells and have the potential for differentiation via adipogenic, osteogenic and chondrogenic pathways. ADMSCs are also known to have high biocompatibility and the ability to combine with other bone material. Purpose: The purpose of this study was to determine the cytotoxicity and characteristics of DDM scaffolds derived from bovine teeth in the ADMSCs of rats cultured in vitro. Methods: This research constituted an experimental study. ADMSCs were isolated from the inguinal fat of rats. Thereafter, DDM was extracted from bovine teeth and formed 355-710 μm-sized particles. DDM scaffolds were assessed using SEM and the effects of DDM scaffolds on the cell viability of ADMSCs at concentrations of 10%, 50%, and 100% analyzed by means of 3-4,5’dimethylihiazol-2-yl,2.5-di-phenyl-tetrazolium bromide (MTT) assay. The results obtained were then analyzed by an ANOVA to establish the difference between the groups. Results: SEM results showed the diameter sizes of the dental tubulis DDM scaffolds to be approximately 4.429 μm and 7.519 μm. The highest cell viability (97.08%) was found by means of an MTT test to be in ADMSCs at a concentration of 10% compared to those at concentrations of 50% and 100%. Conclusion: In conclusion, DDM scaffold derived from bovine teeth with a particle size of 355-710 μm produces a low cytotoxicity effect on ADMSCs.