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Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line Widowati, Wahyu; Mozef, Tjandrawati; Risdian, Chandra; Ratnawati, Hana; Tjahyani, Susy; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of  C.  roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%.  Meanwhile doxorubicin at concentration of 10  μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using  in vitro assay: inhibition of  2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging  activity. Antioxidant activity of  C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.  The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL  and  94.178  μg/mL, respectively. We suggest that  C. roseus extract had a potential anticancer activity by inducing apoptosis.
Antioxidant Potential of Black, Green and Oolong Tea Methanol Extracts Widowati, Wahyu; Herlina, Tati; Ratnawati, Hana; Constantia, Gabriella; Deva, I Dewa Gde Sathya; Maesaroh, Maesaroh
Biology, Medicine, & Natural Product Chemistry Vol 4, No 2 (2015)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.897 KB) | DOI: 10.14421/biomedich.2015.42.35-39

Abstract

Degenerative diseasesandchronicdiseasesare oftencausedbyoxidativestress. Oxidative stress caused by free radicals. Antixodant as inhibitor are needed to prevent it which is one of antioxidant sources is tea. Tea processinggenerally produce various kinds of teas such as black, green and oolong tea. Tea processing  affect thecontent ofphenolic compounds. The aim of the research is to evaluate phytochemical content, totalphenolic content of blacktea, green tea andoolongtea extracts using catechin, quercetin, kaempferol, myricetin as standard, and to evaluate the antioxidative potency of black tea, green tea and oolong tea extracts compared to catechin, quercetin, kaempferol, myricetin. Phytochemical assay using modified Farnsworth method, the antioxidant activity were measured by  by its 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity. Green tea extract contained highest phenolic and flavonoid.  The highest antioxidant activity was green tea extract with IC50=0,487μg/mL. Green tea extract content phenol and flavonoid are higher compared to the other extracts,  green tea extract has the highest antioxidant activity.
The Antiplatelet Aggregation Effect of Extract And Ethyl Acetate Fraction of Velvet Bean Seed (Mucuna pruriens L.) in Dyslipidemic Rat Widowati, Wahyu; Ratnawati, Hana; Rusdi, Udju Djunaedi; Winarno, Wahyu; Kasim, Felix
Agritech Vol 31, No 1 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.31 KB) | DOI: 10.22146/agritech.9726

Abstract

Cardiovascular disease (CVD) is the first cause of death in the world, CVD has complex and multifactorial process including atherogenic lipoprotein, oxidized low density lipoprotein (LDL), endothelial dysfunction, plaque stability, vascular inflammation, thrombotic and fibrinolytic disorder, exercises and genetic factor. Inhibiting the platelet ag- gregation is one of the CVD prevention. Velvet bean seed (Mucuna pruriens L.) can be found abundantly in Indonesia, but has not been used as herbal medicine. Ethanol extract and ethyl acetate of velvet bean seed contain high flavonoids and antioxidants properties which is expected could inhibit platelet aggregation. The objectives of the research were to determine the activity of ethanol extract and ethyl acetate fraction of velvet bean seed towards clotting and bleeding time in dyslipidemic rats. This research used completely randomized design in dyslipidemic rats which were given by ethanol extract of velvet bean seed at the concentrations of 50, 100 and 200 mg/kg BW/day and ethyl acetate fraction of velvet bean seed at the concentrations of 15, 30 and 60 mg/kg BW/day and 42.2 mg/kg BW/day aspirin for ten days. Clotting and bleeding time were measured at days 0, 10, and 20. Data were analyzed using One way analysis of vari- ance and continued with Duncan’s post Hoc test with 95 % level of significancy. The results showed that administration of 60 mg/kg BW/day ethyl acetate fraction of velvet bean seed and at the concentrations of 100 and 200 mg/kg BW/ day ethanol extract of velvet bean seed, prolong the clotting time at day 10, ethyl acetate fraction at the concentration of 60 mg/kg BW/day, 200 mg/kg BW/day ethanol extract of velvet bean prolong bleeding time at day 10.
Prevalence and Characteristics of Liver Cancer Patients in Immanuel Hospital Bandung within January 2013 until December 2014 Period Permadi, Achmad R.; Ratnawati, Hana; Wargasetia, Teresa L.
Journal of Medicine and Health Vol 1, No 4 (2016)
Publisher : Maranatha Christian University

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Abstract

Liver cancer is the fifth most common cancer in Indonesia. This research is to find out the prevalence and characteristics of liver cancer patients in Immanuel Hospital Bandung within the January 2013 until December 2014 period based on age, gender, clinical symptoms and predilections. This study was a descriptive verificative research with data retrieval of patients medical records that have been diagnosed with liver cancer that were hospitalized in Immanuel Hospital Bandung within January 2013 until December 2014 period. The study showed that the liver cancer patient prevalence in Immanuel Hospital Bandung within the period of January 2013 until December 2014 was 46 people. Characteristics of liver cancer patients in Immanuel Hospital Bandung within January 2013 until December 2014 period showed that the most liver cancer patients were male, compare with female with ratio 4:1, the most common age group of 56-65 years old, the most common clinical symptoms were abdominal pain with or without reffered pain to the right scapular bone and the most common predilection was right lobe of the liver. Key words: liver cancer, patients characteristics, prevalence 
Lung Cancer Patient Description in Immanuel Hospital Bandung from January 2013 to December 2014 Bulan, Ida AKT; Ratnawati, Hana; Wargasetia, Teresa L
Journal of Medicine and Health Vol 1, No 6 (2017)
Publisher : Maranatha Christian University

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Abstract

Lung cancer is a disease which has high incidence and mortality rate. It makes lung cancer become one of the biggest public health problems. The aim of this study is to find out the description of lung cancer patients in Immanuel Hospital Bandung from January 2013 to December 2014 based on the prevalence, gender, age, clinical features, metastasis, and mortality percentage. The method design was a descriptive retrospective method with data retrieval and collection of patient medical records that have been diagnosed with lung cancer in Immanuel Hospital Bandung from January 2013 to December 2014 period. This study showed that lung cancer prevalence was 51 patients, the majority of patients were males compared to females with the ratio of 2.7:1, the most common age group was 61–70 years old, the most common clinical features were dyspnea and cough, the most common site of metastasis was liver, and the mortality percentage was 33.33%.Keywords: Immanuel hospital Bandung, lung cancer, patient’s description
POTENCY OF ANTIOXIDANT, ANTICHOLESTEROL AND PLATELET ANTIAGGREGATION OF BLACK TEA (Camelia sinensis ) Widowati, Wahyu; Herlina, Tati; Ratnawati, Hana; Mozef, Tjandrawati; Immanuel, Victor
Buletin Penelitian Tanaman Rempah dan Obat Vol 22, No 1 (2011): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v22n1.2011.%p

Abstract

Epidemiological studies have shown an in-verse correlation betweendiets rich in po-lyphenols, flavonoids and reduced risk of cardiovasculardisease. These associationswere mainly ascribed to the antioxidant, antiaggregation and anticholesterol capa-city of polyphenols. This association has been explained that atherogenesis is ini-tiated by hypercholesterol, lipid peroxida-tion and hyper aggregation platelet. The research was carried out to evaluate anti-oxidant, anticholesterol and antiaggrega-tion activities of methanol extract and frac-tions of black tea (Camelia sinensis). To evaluate antioxidant activity of methanol extract and fractions were compared  with (-)-Epigallocatechine 3-gallate (EGCG), an-ticholesterol activity were compared with simvastatin and antiaggregation activity were compared with aspirin. Antioxidant activity using the 1,1-diphenyl-2-picryl-hy-drazyl (DPPH) free radical scavenging acti-vity at two concentrations 100 µg/mL, 50 µg/mL and anticholesterol was cholesterol oxidase activity at two concentrations 0.005 µg/mL, 0.0025 µg/mL, the antiag-gregation  activity  used epinephrine (EPN)agonist at two concentrations 300 μM, 75 μM and one concentration 37.1 µg/mL of methanol extract and fractions of black tea. To know the difference of treatment, the data were analysed using Analysis of Variance (ANOVA) and be continued Dun-can’s new multiple range test. The results showed that DPPH scavenging activity were high (88.59-93.556%), the anticho-lesterol showed high activity (93.663-97.434%) and the antiaggregation sho-wed low up to high activity (79.967-4.31%). Using Duncan’s new multiple ra-nge test showed that anti-oxidant activity were not different among extract and fractions of black tea,the highest anti-cholesterol activity was black tea extract 0.005 µg/mL (97.434%) and the highest antiaggregation activity was ethyl acetate fraction on 75 μM EPN agonist (4.310 %), water fraction 37.1 µg/mL with 300 μM EPN agonist (21.833%).
Lung Cancer Patient Description in Immanuel Hospital Bandung from January 2013 to December 2014 Bulan, Ida AKT; Ratnawati, Hana; Wargasetia, Teresa L
Journal of Medicine & Health Vol 1 No 6 (2017)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.773 KB) | DOI: 10.28932/jmh.v1i6.548

Abstract

Lung cancer is a disease which has high incidence and mortality rate. It makes lung cancer become one of the biggest public health problems. The aim of this study is to find out the description of lung cancer patients in Immanuel Hospital Bandung from January 2013 to December 2014 based on the prevalence, gender, age, clinical features, metastasis, and mortality percentage. The method design was a descriptive retrospective method with data retrieval and collection of patient medical records that have been diagnosed with lung cancer in Immanuel Hospital Bandung from January 2013 to December 2014 period. This study showed that lung cancer prevalence was 51 patients, the majority of patients were males compared to females with the ratio of 2.7:1, the most common age group was 61–70 years old, the most common clinical features were dyspnea and cough, the most common site of metastasis was liver, and the mortality percentage was 33.33%.Keywords: Immanuel hospital Bandung, lung cancer, patient’s description
Prevalence and Characteristics of Liver Cancer Patients in Immanuel Hospital Bandung within January 2013 until December 2014 Period Permadi, Achmad R.; Ratnawati, Hana; Wargasetia, Teresa L.
Journal of Medicine & Health Vol 1 No 4 (2016)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.433 KB) | DOI: 10.28932/jmh.v1i4.531

Abstract

Liver cancer is the fifth most common cancer in Indonesia. This research is to find out the prevalence and characteristics of liver cancer patients in Immanuel Hospital Bandung within the January 2013 until December 2014 period based on age, gender, clinical symptoms and predilections. This study was a descriptive verificative research with data retrieval of patients medical records that have been diagnosed with liver cancer that were hospitalized in Immanuel Hospital Bandung within January 2013 until December 2014 period. The study showed that the liver cancer patient prevalence in Immanuel Hospital Bandung within the period of January 2013 until December 2014 was 46 people. Characteristics of liver cancer patients in Immanuel Hospital Bandung within January 2013 until December 2014 period showed that the most liver cancer patients were male, compare with female with ratio 4:1, the most common age group of 56-65 years old, the most common clinical symptoms were abdominal pain with or without reffered pain to the right scapular bone and the most common predilection was right lobe of the liver. Key words: liver cancer, patients' characteristics, prevalence 
PHYTOCHEMICAL ASSAY AND ANTIPLATELET ACTIVITY OF FRACTIONS OF VELVET BEAN SEEDS (MUCUNA PRURIENS L.) WIDOWATI, WAHYU; RATNAWATI, HANA; RUSDI, UDJU DJUNAEDI; WINARNO, WAHYU; IMMANUEL, VICTOR
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.207 KB) | DOI: 10.4308/hjb.17.2.85

Abstract

Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations  (300, 150, 75, and 30 ?M), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 ?M of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 ?M).
Free Radical Scavenging and α-Glucosidase Inhibitor Activity of Ethanolic Extract of Mucuna pruriens L. Widowati, Wahyu; Ratnawati, Hana; Retnaningsih, Ch.; Lindayani, .; Rusdi, Udju D.; Winarno, Wahyu
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Biji kacang “koro benguk” atau velvet bean sudah sangat dikenal tetapi dasar ilmiah penggunaannya sebagai obat tradisional terutama sebagai antioksidan dan antidiabetik masih belum diketahui. Oleh sebab itu dalam penelitian ini dilakukan percobaan untuk mengevaluasi aktivitas antioksidan dan antidiabetik dari ekstrak biji koro benguk, serta fraksi heksana, etil asetat, butanol dan air menggunakan uji in vitro menggunakan DPPH (1,1-difenil-2-pikril-hidrazil) dengan pembanding positif kuersetin dan BHA. Hasil penelitian menunjukkan bahwa IC50 DPPH untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol dan air berturut-turut sebesar 22,17; 152,60; 113,03; 4,93; dan 9,14 μg/mL, sedangkan untuk kuersetin dan BHA masing masing sebesar  4,28 dan 22,587 μg/mL. Aktivitas antidiabetik ditentukan menggunakan metode penghambatan α-glukosidase dengan glucobay sebagai kontrol positif. Hasil penelitian menunjukkan bahwa IC50 antidiabetik untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol, air, dan glucobay berturut-turut sebesar 24,44; 19,55; 8,40; 80,98; 48.97; dan 16,23 μg/mL. ABSTRACT Velvet bean seeds are ubiquitous but the scientific basis for its medicinal use especially as antioxidant and antidiabetic remains unknown. Therefore, a study was designed to evaluate  antioxidant and antidiabetic activities of the  extract, hexane, ethyl acetate, butanol and water fractions of velvet bean seeds. In order to evaluate antioxidant activity of extract and fractions  using  quercetin,  butylated hydroxyanisole (BHA) as positive control and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity was determined. The results showed that IC50 of DPPH were 22.17; 152.60; 113.03; 4.93; 9.14 μg/mL respectevily;  quercetin 4.28 and BHA 22.587 μg/mL.  Antidiabetic activity was determined by using  of α-glucosidase inhibition assay of  extract, four fractions and glucobay as positive control. The results showed that antidiabetic IC50 were24.44; 19.55; 8.40; 80.98; 48.97μg/mL respectively and glucobay as positive control  16.23 μg/mL.