Didik Pudji Restanto
Plant Tissue Culture Laboratory Agrotecnology Department Faculty of Agriculture Jember University, CDAST Jember University and Biology Department Faculty of Mathematics and Natural Sciences Jember University

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MORPHOLOGICAL DIVERSITY AND MOLECULAR RAPD MARKERS OF SUGARCANE MUTANE (SACCHARUM OFFICINARUM L.) IN INUNDATION TOLERANCE Avivi, Sholeh; Suliswanto, Eko Nur; Restanto, Didik Pudji; Miswar, Miswar; Syamsunihar, Anang; Soeparjono, Sigit; Hartatik, Sri
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1304

Abstract

The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7 - 56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.
ENHANCEMENT OF REGENERATION EFFICIENCY THROUGH CALLUS INDUCTION MEDIA USING 2,4-DICHOLOROPHENOXYACETIC ACID IN INDICA RICE (ORYZA SATIVA L. VAR. CIHERANG) Azizah, Kunti Anis; Restanto, Didik Pudji; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1379.154 KB) | DOI: 10.19184/jid.v18i2.2592

Abstract

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it  is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS  basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l,  BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS  basal, NAA 0,2 mg/l, Kinetin 2 mg/l,  Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS  basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus  
CLONING COAT PROTEIN GENE OF CBSD (CASSAVA BROWN STREAK DISEASE) AT CASSAVA (MANIHOTESCULENTUM) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
PENGARUH UKURAN EMBRIOZIGOT TERHADAP REGENERASI BEBERAPA KLON KAKAO Avivi, Sholeh; Restanto, Didik Pudji; Widyastuti, Tri
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.245 KB) | DOI: 10.31258/jnat.13.3.237-243

Abstract

This sesearch identified the responses of size of zygotic embryo of cocoa and to identify the responses of cocoaclone types toward ability to produce somatic embryo. The research was designed by Completely RandomizedFactorial Design within five replications. The first factors were the sizes of used explants, namely small (2-5 mm),average (6-9 mm) and big (10-15 mm). The second factors were cocoa clones, namely RCC 72, Sca 6, KW 162, KW163 and KW 165. The result showed that the best explant was the small size (2-5 mm) of cocoa zygotic embryo. Ithad significantly on number of rooting embryos and number of embryos with buds on rooting media. Clone RCC 72was the most responsive clone and could generate to be plantlet. Clone KW 165 performed the lowest response.
Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
PENGARUH KONSENTRASI 2,4 – DICHLOROPHENOXYACETIC ACID (2,4-D) TERHADAP INDUKSI KALUS TANAMAN SORGUM Maulana, Rizqi; Restanto, Didik Pudji; Slameto, Slameto
JURNAL BIOINDUSTRI Vol 1, No 2 (2019): Jurnal Bioindustri Volume 1 Edisi 2 - Mei 2019
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v1i2.223

Abstract

Rendahnya keragaman genetik dan produktivitas tanaman sorgum menjadi permasalahan dalam pengembangan tanaman sorgum. Oleh karena itu, dilakukan pendekatan bioteknologi terutama untuk mendapatkan tanaman transgenik. Pendekatan bioteknologi didukung oleh bahan tanam yang baik dengan menggunakan teknik kultur jaringan untuk mendapatkan kalus tanaman sorgum. Perbanyakan kalus dilakukan dengan menggunakan metode kultur jaringan dengan menambahkan konsentrasi zat pengatur tumbuh 2,4-D. Penelitian ini dilakukan di Laboratorium Kultur Jaringan Tanaman Fakultas Pertanian Universitas Jember. Penelitian ini disusun dengan menggunakan metode Rancangan Acak Lengkap (RAL) dengan faktor tunggal yaitu pemberian 2,4-D berbagai konsentrasi antara lain A0 (kontrol), A1 2 ppm, A2 3 ppm, A3 4 ppm, A4 5 ppm, dan A5 6 ppm dimana setiap perlakuan diulang 3 kali. Variabel pengamatan yang dilakukan secara kuantitatif yaitu perhitungan kedinian kemunculan kalus, jumlah kalus, dan berat kalus. Variabel pengamatan secara kualitatif yaitu menentukan warna kalus dan tekstur kalus yang diamati secara visual. Hasil dari penelitian ini menunjukkan berbeda sangat nyata pada setiap pengamatan kuantitatif dan menunjukkan hasil terbaik pada pengamatan kualitatif. Perlakuan 2 ppm 2,4-D menunjukkan hasil terbaik dibandingkan perlakuan 2,4-D yang lain yaitu mampu menginduksi kalus tanaman sorgum cenderung lebih cepat yaitu 7,6 HST, presentase jumlah kalus 90%, berat kalus 0,6 gram serta kalus berwarna putih kekuningan (5Y 8/6) dan kalus bersifat friable (remah).
THE PROPAGATION OF ORCHID THROUGH FORMATION OF PROTOCORM LIKE BODIES (PLB) FOR SUPPORTING THE RESQUE OF Phalaenopsis ORCHID IN INDONESIA Kriswanto, Budi; Soeparjono, Sigit; Restanto, Didik Pudji
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Indonesia is a tropical country that has large orchid germ plasm because it has tropicalforests along the equatorial regions (islands of Sumatra, Kalimantan, Sulawesi and Papua) Recently, there has been changed become oil palm plantations and climate change, which hascaused the extinction of orchid. The Phalaenopsis orchid is an orchid that has high exotic andcommercial value. The purpose of this study was to maintain the Phalaenopsis throughpropagation of Protocorm Like Bodies (PLB). This study used the treatment of different kindsof basal medium Murashige & Skoog (MS), ½ Murashige & Skoog (1/2 MS) and Vacin& Went(VW) and 6-benzylaminopurine (BAP) and ?-naphthylacetic acid (NAA) concentrations ongrowth and propagation of PLB. The results showed sequentially VW, ½ MS and MS mediaprovided the best plb growth and media ½ MS provides regeneration plb into a good planletin which shoots and roots formed. Treatment of plant growth regulator of BAP: NAA effect ondiameter of plb colony and individual size of plb with 2.1 cm and 0.2 cm, respectively.
MORPOLOGICAL CHARACTERISTICS AND POLLINIA OBSERVATION OF 10 INDONESIA NATIVE DENDROBIUM ORCHIDS Indraloka, Aldy Bahaduri; Dewanti, Parawita; Restanto, Didik Pudji
BIOVALENTIA: Biological Research Journal Vol 5, No 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/BIOV.5.2.2019.140

Abstract

Characterization of native orchid (species) in Indonesia is important for preservation of germplasm and selection process to obtain dominant character of orchids as potential parental. Morphological characterization is a method to obtain scientific information in phenotypic manner. Characterization of plants was carried out on 10 native orchids of Dendrobium genus to obtain specific character. Characterization process based on UPOV guidebook and morphological observation of pollinia was also carried out on 10 native orchids to obtain information about the structure and physiology of pollinia. The results of morphological characterization on 10 native orchid showed several qualitative characters such as flowering position, cross section of leaf, pseudobulb firmness, and leaf arrangement in D. Canaliculatum was different from 9 other native orchid. Morphological pollen of 10 native orchid shows the diversity structure of pollinia and the physiological conditions of mature pollen which are ready to pollinate are pollinia with a golden yellow colored. Knowledge and information about parental relationships is the main point for developing ornamental plant breeding programs. Selection of native orchids as potential elder is important where selected elders will be used as a source of genetic diversity. The results of this study are expected to provide scientific information and phenotypic selection for the development of hybrid orchids.