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POLIMORFISME PENANDA RAPD UNTUK ANALISIS KERAGAMAN GENETIK PINUSMERKUSII DI HUTAN PENDIDIKANUNHAS Gusmiaty, Gusmiaty; Restu, Muh.; Asrianny, Asrianny; Larekeng, Siti Halimah
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.822 KB) | DOI: 10.31258/jnat.16.2.47-53

Abstract

The aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.
POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE Larekeng, Siti Halimah; Restu, Muh.; Gusmiaty, Gusmiaty; Rismawati, Rismawati
AgroTech Journal Vol 1, No 1 (2016): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2168.934 KB) | DOI: 10.31327/atj.v1i1.173

Abstract

Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
SELEKSI PRIMER UNTUK ANALISIS KERAGAMAN GENETIK JENIS BITTI (Vitex coffassus) Gusmiaty, . ; Restu, Muh. ; Pongtuluran, Ira
PERENNIAL Vol 8, No 1 (2012)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v8i1.211

Abstract

The successful of genomic DNA amplification using RAPD technique was determined by the sequence of primer’s base and primer compound in each reaction. The aim of research was finding the best primer for genetic diversity analyses of Bitti (Vitex coffassus). The results of amplification showed that number of band between one to five bands (110-600 bp). The primer of OPK-10, OPA-17, OPQ-07 and OPP-08 can be used for genetic diversity analyses of Bitti. The best primer used for genetic diversity analyses of Bitti was OPP-08 because it had the highest number of bands (5 bands). Key words: OPP-08, Primer Selection, Vitex coffassus
PENGARUH DOSIS INOKULAN ALAMI (EKTOMIKORIZA) TERHADAP PERTUMBUHAN SEMAI TENGKAWANG (Shorea pinanga) Gusmiaty, . ; Restu, Muh. ; Lestari, Ayu
PERENNIAL Vol 8, No 2 (2012)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v8i2.217

Abstract

This research aimed to know the effect of some doses of natural inoculants on the growth of Shorea pinanga seedling. The research was carried out with several steps including seed provision, inoculation of ectomycorrhyza, planting and maintenance in the nursery. The result of the research showed that height and number of branches were different at the significant level of 5 %, while diameter and number of leaves were not significantly different. The natural inoculants dose of 15 g showed the best growth in response to the increment of height, diameter, and number of leaves, while that of 20 g showed the best in the number of branches increment. Key words: Shorea pinanga, ectomycorrhyza, seedling, natural inoculantstor, Local Government Revenue, regional autonomy
PRODUKSI POLONG DAN BIJI TANAMAN GAMAL (Glirisidia sepium) DARI BERBAGAI PROVENANSI DENGAN PEMUPUKAN NPK Restu, Muh.; Mappangaja, Baharuddin
PERENNIAL Vol 2, No 1 (2006)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v2i1.150

Abstract

The productivity of forest natural resources is now decreasing and therefore rehabilitation efforts is urgently needed. Breeding of forest plant is the best solution to increase forest productivity in both quantity and quality. The biological reproduction can be conducted through seed production with fertilization. This research was aimed at determining the effect of fertilizing treatments on pod and seed production of different Glirisidia sepium provenances to find out the best provenance and best fertilizer dosage. Fertilization treatments showed a significant effect on the pod and seed production. On the other hand, the provenance and its interaction with fertilizing did not show any significant effects on the pod and seed production. The best performance was found on the fertilization treatment of 500 g resulting in the average production of 1,288 pods and 27,621 seeds/tree. Keywords: Fertilizing, Seed Production, Provenance, Glirisidia sepium
POTENSI DAN PERMUDAAN TEGAKAN ALAM EBONI (Diospyros celebica Bakh.) DI AREAL HPH PT. INHUTANI I MAMUJU Restu, Muh.
PERENNIAL Vol 2, No 2 (2006)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v2i2.160

Abstract

The intensive use of Eboni has caused a decrease of its natural population which has also contributed to the future loss of genetic potencies. Preservation efforts could only be conducted if the actual potencies of the stand and its environmental conditions were recognized. A study on stand and regeneration potencies as well as its soil characteristics is needed as a basic in management aplication for the increase of genetic potencies. This study used a systematic sampling survey method and laboratory analysis. The study aims to identify tree potencies and regeneration of Eboni Makassar as well as its soil characteristics under the stand. The results are expected to provide a description on Eboni Makassar potencies and its management efforts. It was found from the study that the potency of Eboni was 5.013 m3/ha with minimum and maximum estimated volumes of 3.163 m3/ha and 6.863 m3/ha, respectively. Eboni was naturally regenerated better in >40% slope category. Keywords: Eboni, Potencies and Regeneration, Various Slope