Articles

SENSITIVITY OF PROPOLIS SOLUTIONS ON AEROMONAS HYDROPHILA BACTERIA Bako, Surandha; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Perikanan dan Kelautan - UNRI Vol 24, No 2 (2019)
Publisher : Universitas Riau

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GAMBARAN DARAH IKAN NILA (OREOCHROMIS NILOTICUS) DI KOLAM BUDIDAYA DI KECAMATAN MARPOYAN DAMAI KOTA PEKANBARU Riauwaty, Morina; Syawal, Henni
Jurnal Perikanan dan Kelautan - UNRI Vol 21, No 1 (2016)
Publisher : Universitas Riau

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The aim of the research is to describe the blood of Tilapia that had been cultured in a fishpond since June to December 2015. The ponds were located in Marpoyan Subdistrict, Pekanbaru city. Twenty samples were collected as much as 20 fish that consisted of 10 male and 10 female with the size ranging from 9.5 ? 18.2 cm. The blood of the fish was taken with syringe via caudal vena as much of 1 ml. The blood parameters then were calculated in term of the number of hematocrit, leucocyte, erythrocyte, and leukocyte. The results showed that the amount of hematocrit and leukokrit were ranged between 10 ? 31 % and 1.78 ? 3.10 % consecutively. The total cell of erythrocyte ranged between 1,170,000 ? 2,190,000 cell/mm3, and the total of leukosit were about 81,000 ? 105,000 cell/mm3. In addition, the types of leukocyte found at this study were hepatocyte (53- 86%), thrombocyte (25 ? 39%), monocyte and neutrophil between 1 ? 3 %.
THE EFFECT VITAMIN C ENRICHED PELLETS TO INCREASE THE IMMUNITY OF OREOCHROMIS NILOTICUS Wirdona, Maytra; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTVitamin C is a nutrient that is soluble in water and is essential for life as well as for maintaining health, enhancing immunity of fish in fighting infections and as an antioxidant. The study aims to obtain the best dose of vitamin C on total leukocytes, leukocyte differentiation, and phagocytosis activity to increase the immunity of nile tilapia towards disease attack Streptcoccosis. The method used is the experimental method by using the full random draft (RAL) One factor with five levels of treatment and the treatment is repetead three times. After the treatment was applied, namely Kn (without vitamin C enriched pellets and not challenged), Kp (pellets without vitamin C enriched and challenged), vitamin c enriched pellets at a dose of P1 (1 g/kg and challenged with Streptococcus agalactiae), P2 (1.5 g/kg and challenged with S. agalactiae), P3 (2 g/kg and challenged with S. agalactiae). Nile tilapia used in size 8-10 cm with solid tebar 1 tail/3 L water is maintained in the aquarium with a size of 40 x 30 x 30 cm with a feed enriched with vitamin C for 46 days. The fish was challenged with S. agalactiae with a density 108 CFU/mL at the dose of 0.1 mL/tail intramuscular on day 32 after that the fish re-maintained until the 46 days. Measured parameters are total leucocytes, leucocyte differentiation, phagocytic activity and survival rate of nile tilapia. The results shown that the feed enriched with vitamin C and tested with S. Agalactiae has an influence on the immunity of fish with the best treatment in P3 (the vitamin C enriched pellets at a dose of 2 g/kg and challenged) with an average total leukocytes was 8.02 x 104 cells/mm3, lymphocytes were 84.67%, neutrophils was 8.67%, monocytes were 6.66%, phagocytosis activity is 46.00% and the livelihood reaches 93.33%.Keywords : Leukocytes, Tilapia, Vitamin C, Streptococcus agalactiae, Phagocytosis 
HEMOTOLOGICAL OF CLARIAS GARIEPINUS THAT WERE GIVEN WITH VACCINE TO PREVENT MOTILE AEROMONAS SEPTICEMIA DISEASE Triyatna, Deny; Lukistyowati, Iesje; Riauwaty, Morina
Berkala Perikanan Terubuk Vol 47, No 3 (2019): NOVEMBER 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

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Abstract

The vaccine is one of the alternative to improve infection of the immunity system. The purpose of this research was to understand the hematological of (Clarias gariepinus) that were with given Aeromonas hydrophila vaccine. This research was conducted on 14 April until 2 June 2019. The method was an experimental method with a Completely Randomized Design (CRD) five treatments and three times replication. The treatments appliced were: Kn: (The fishes that were not given with vaccine and infected by Aeromonas hydrophila), Kp: (The fishes that were not given vaccine and was infected by Aeromonas hydrophila, P1: Soaked with vaccine dose 1 ml/10 L, P2: Soaked with vaccine dose 1 ml/10 L, P3: (Dipping with vaccine dose 1 ml/10 L. Dipping with the vaccine is done for 15 minutes. Clarias gariepinus at average 8-10 cm TL. After 23 day, the fishes were infected with Aeromonas hydrophila, the purpose of which was to determine imumunity of fish. The results show that the vaccine for 15 minutes is able to prevent the Motile Aeromonas Septicemia (MAS) disease. The were range total erythrocyte is 2,29×106 sel/mm3, hemoglobin 12,60 g/dl, Leukocytes Differensial (Lymphocytes 71,33%, Monocytes 16,66% and Neutrophils 12,00%) and Survival Rate 63,3%.
Differentiation Of Leukocytes Cat Fish (Mystus Nemurus) Fed Solutions Containing Curcuma (Curcuma Xanthorrhiza) Siagian, Juandi Riki Umbara; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The effect of containing curcuma (Curcuma xanthorrhiza Roxb) on diferentiation of leukocytes of Cat Fish (Mytus nemurus). The aim of the study is to find of diferentiation of leukocyte of Cat Fish (Mytus nemurus). The study was conducted from March to April 2014 in the Experimental Pond and Laboratory of Parasitic Diseases of Fish and Fisheries and Marine Sciences Faculty of the Riau University. The method used was experimental method using RAL with one factor with 4 level treatments and three repliations. the result indicate that the best differentiation of leukocytes in the P3 treatment is total leukocytes (96.538 cell/mm3), diferentiation of leukocytes (lymphocytes: 96,67%, monocytes: 1,67%, and neutrophils: 1,67%) and the best treament of survival rate is P2 and P3 with survival rate is 100%.Keywords: Differentiation, Leukocytes, Cat Fish, and Curcuma Solution
Histology of Liver and Kidney of Mystus nemurus that immersed with Curcuma xanthorrhiza, ROXB extract Lubis, Fauzi Ahmad; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was carried out in November 2013 until January 2014 in the laboratory of Fish Diseases and Parasites, Faculty of Fisheries and Marine Science Riau University Pekanbaru. The purpose of this research was to understand the histological structure of liver and kidney of Mystus nemurus that is immersed with Curcuma xanthorrhiza ROXB extract. The method used in this study was experimental and the histologycal structure of the fish will be analyzed descriptively. The treated fish were immerse in 3 different concentration of Curcuma xanthorrhiza ROXB extract, they were (P1 (0.2 g/L), P2 (0.4 g/L, P3 (0.6 g/L). for five minutes/ day for 30 days period. The negative control were fishes that were not treated with Curcuma xanthorrhiza ROXB extract. The liver showed hemorhage, fatty degenerative and necrosis. The kidney showed necrosis and hemorhage. It was concluded that immersion of fish in Curcuma xanthorrhiza ROXB extract shown histologycal alterations in the tissue of Mystus nemurus.Key words: Mystus nemurus, Curcuma xanthorrhiza ROXB, kidney, liver
THE EFFECTS OF PHOTOPERIOD MANIPULATION ON MORPHO-ANATOMY AND GROWTH OF CLARIAS GARIEPINUS Lubis, Sandra; Windarti, Windarti; Riauwaty, Morina
Berkala Perikanan Terubuk Vol 46, No 3 (2018): November 2018
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.838 KB) | DOI: 10.31258/terubuk.46.3.60-68

Abstract

Changing in photoperiod duration will affects the physiology of fish in general. A study aims to understand the effects of photoperiod manipulation towards C. gariepinus morpho-anatomy and growth was conducted on March?May 2018. The fishes were reared in fiber tanks (140 x 60 x 40 cm) that was filled with water and completed with a circulatory system. Treatments applied were Control (natural photoperiod), 24G (24 hours dark), 18G6T (18 hours dark and 6 hours light), and 6G18T (6 hours dark and 18 hours light). The fishes were sampled once/14 days, 4 fishes/ aquarium/sampling. Parameters measured were length-weight relationship, condition factor, hepatosomatic index (HSI), viscerasomatic index (VSI), absolute length and weight, food conversion ratio (FCR), gonad development and body protein/fat. Results shown that photoperiod manipulation affects C. gariepinus morpho-anatomy and growth. The highest growth parameters and morpho-anotomy index were found in fish reared in 18G6T. By the end of the experiment, the b value of   length-weight relationship was 3.3205, absolute weight and length were 86.70 g and 17.23 cm, condition factor was 0.84, VSI was 25.31%, HSI was 11.42%, FCR was 1.43, and body protein and fat was 48.40% and 50.38%. In the fish reared in 24G treatment, b was 1.9262, 52.13 g BW and 14.19 cm SL, condition factor was 0.80, VSI was 23.30%, HSI was 9.38%, FCR was 2.47, and body protein and fat was 48.08% and 49.53%. In the fish reared in 6G18T, b was 2.6903,49.45 g BW and 13.79 cm SL, condition factor was 0.79, VSI was 23.30%, HSI was 6.80%, FCR was 1.91, and body protein and fat was 46.64% and 49.00%. Fish reared in natural photoperiod (control) shown the lowest results b was 2.0418, 51.68 g BW and 14.21 cm SL, condition factor was 0.79, VSI was 19.58%, HSI was 6.96%, FCR was 2.20, and body protein and fat was 43.95% and 47.77%.Early developing gonad (1st maturity stage) was only found in fish reared in 18G6T. Based on data obtained it can be concluded that the photoperiod manipulation affect the morpho-anatomy and growth of C. gariepinus.
THE EFFECT OF THE ADDITION OF GINGER (Curcuma xanthorrhiza ROXB) TO FEED TO TOTAL ERYTHROCYTES, HEMATOCRIT, HEMOGLOBIN and GROWTH OF CATFISH (Mystus nemurus) Yulistia, Feri; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was done in March - April 2014 in Fish Disease Parasite and Laboratories and an Experiment of the Faculty of Fisheries and Marine Science University of Riau. The purpose of this research is to know the influence of the addition of curcuma on feed to the total of erythrocytes , hematocrit , hemoglobin and the growth of catfish. A research method used is the design of random complete with one factor which feed containing curcuma with four standard treatment consists of , P0: control without given ) curcuma solution , the P1 ( curcuma solution with a dose of 2 g/kilogram feed ) , the P2 ( curcuma solution with a dose of 4 g/kilogram feed ) , the P3 ( curcuma solution with a dose of 6 g/kilogram feed ). The results of research shows that the addition of a solution on feed curcuma doesn’t impact the condition of fish blood because of poor environmental conditions. It can increase the growth of absolute weight catfish .The total erythrocytes is ranged between 1,25-1,91 mm3/ million cells , hematocrit 13,8-18,5 % and the percentage of hemoglobin 3,9-4,2 g/dL.Keywords: River catfish (Mystus nemurus), ginger, blood catfish
IDENTIFICATION OF PATHOGENIC BACTERIA OF TILAPIA (Oreochromis niloticus) IN SUBDISTRICT MARPOYAN DAMAI PEKANBARU Lubis, Dicky Azwar; Syawal, Henni; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was done in April - Juni 2014 in Fish Disease Parasite and Laboratories and an Experiment of the Faculty of Fisheries and Marine Science University of Riau. This study aims to determine the types of pathogens that attack tilapia in District Marpoyan Damai Pekanbaru. The benefits of this research is to provide information to farmers about the types of pathogens in tilapia, so it can be the prevention of disease. The method used in this study is a survey method by taking samples in the field and analyzed in the laboratory. The amount of bacteria found from both the ponds was six species. Bacteria found in a pond one these were Aeromonas hydrophila, Basillus sp, Pseudomonas sp and Streptococcus sp. Meanwhile bacteria found in a pond two these were Basillus sp, Stapyhilococcus sp, and Edwardsiella tarda.Keywords: Pathogenic Bacteria, Tilapia,
LEUKOCYTES DIFFERENTIATION OF Pangasius hypopthalmus THAT WERE FEED WITH CURCUMIN EXTRACT FROM Curcuma domestica V Iman, Khasan Nur; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on June until August 2016 in Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine science, University of Riau, Pekanbaru. The aim of this research was to determine leucocyte differentiation of Pangasius hypopthalmus that were feed with curcumin extract from Curcuma domestica V. This research used five treatment such as; Kn (negative control): that were feed without curcumin extract and were not infected with A. hydrophila, Kp (positive control): that were feed without curcumin extract and infected with A. hydrophila, P1: feed with curcumin extract in 0,3g/kg, P2: feed with curcumin extract in 0,5g/kg and P3: feed with curcumin extract in 0,7 g/kg. Fish reared in an aquarium the size of 40x30x30 cm with stocking density 10 fish / aquarium, feeding as many as 10% of body weight and do 3 times a day. Pisciculture carried out for 30 days and after that fish infected with Aeromonas hydrophila. The results shown that P3 (feed with curcumin extract in 0,7 g/kg) were the best treatment with lymphocytes 83,00%; monocytes 5.33%; neutrophils 3,67%; thrombocyte 8.00%; total leukocytes 12,01x104 cell/mm3; and fagocyte index 58,33.Keyword: Leukocytes, Pangasius hypopthalmus, Curcuma domestica V, Curcumin Extract