Wibi Riawan
Departemen Biokimia dan Biomolekuler, Fakultas Kedokteran, Universitas Brawijaya

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The effect of four weeks restricted diet on serum soluble leptin receptor levels and adipocyte leptin receptor density in normoweight rattus norvegicus strain Wistar Indra, M. R.; Riawan, Wibi
Medical Journal of Indonesia Vol 15, No 3 (2006): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.784 KB) | DOI: 10.13181/mji.v15i3.229

Abstract

One of the five possible mechanisms of leptin resistance in human obesity is the defect in the leptin receptor (Ob-R). Evidence has accumulated that leptin-binding activity in human serum is related to a soluble form of the leptin receptor, and restriction of energy intake resulted a decrease in circulating leptin levels. Aim of this study is to examine the difference of serum soluble leptin receptor level and leptin receptor density in rat adipose tissue of adventitial aorta after four weeks treated with different restricted diets. Soluble leptin receptor level was measured by ELISA and leptin receptor density by using immuno-histochemistry. The soluble leptin receptor in group treated with 40% of normal daily calori diet was found significantly lower than control (p = 0.02). There were no any significant differences among group treated with 40 % of normal daily calori diet, “1 day fast-1day eat”, and ”1day fast-2 days eat” groups, and among 1 day fast-1 day eat”, ”day fast - 2 days eat” and control groups as well. On the other hand, leptin receptor density in adipose tissues was higher in restricted diet group than control. Diet of 40 % normal daily calorie for 4 weeks decreased soluble leptin receptor level, but increased adipocyte leptin receptor density of the adipose tissue of rat adventitial aorta. These changes may be resulted from an up regulation mechanism in relation with homeostatic maintenance. (Med J Indones 2006; 15:145-50) Keywords: restricted diet, leptin receptor, soluble leptin receptor, adipocyte, obesity
PROFIL KADAR SOLUBLE UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR (SUPAR) PADA SERUM PENDERITA TUBERKULOSIS PARU (SEBAGAI MONITORING TERAPI) Astuti, Triwahju; MR, Tri Yudani; Riawan, Wibi; Muktiati, Nunuk Sri; Widjajanto, Edy
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2008.024.01.4

Abstract

Dutch study shows that Upar expression content is significantly higher in tubercolusis patient?s psiphsy monosit compared to those in the healthy control group. So far, there is no biologic marker used whichcan accurately observe response improvement in the treatment of lung tubercolusis. The aim of this research is to investigate whether the serum level of soluble utokinase plasminogen activator receptor (suPAR) carries information in monitoring TB treatment for Lung Tuberculosis patients. suPAR was measured by ELISA in 21 individuals at the time of enrolment into observational cross sectional based on active tuberculosis  and 5 individuals as healthy control. There were 3 groups, 1). patients who had not started treatment (n=7),2). patients who had been treated for 2 months (n=7),  3). patients who had been treated for 6 months (n=7). Among groups, there were no difference in mean of body mass index, erythrocyte sedimentation rate and monocyte count. Patients positive for TB on direct  microscopy were 29% ( 6 from 21 patients) , 2 patients each groups. The suPAR levels were elevated in patients with active TB compared to healthy control (P<0,001). suPAR levels were highest in patients positive for TB on direct microscopy ( mean suPAR 4.455 ng/ml).
YLS-THERAPY: POTENSI SUPERNATAN YOGURT LBA–ST SEBAGAI ANTIKANKER SERVIKS BERBASIS BIOMOLEKULER Permata Sari, Desie Suci; Ridho, Wildan Mochamad; Lizziyyannida, Lizziyyannida; Sholihah, Zurniatus; Putra Pratama, Satya Hanggara; Rahmani, Erita; Riawan, Wibi
Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Abstract

Cervical cancer is the third most diagnosed cancer and the fourth leading cause of cancer death in females in the world. Zebrafish have been used as experimental animals in human tumors because of similar gene expression (70%) than other experimental animals. Yogurt LBA-ST produces SCFA metabolites (acetate, lactate, and butyrate) that can provide anticarcinogenic effects. There has been a vast growth of related research focusing on proliferation and apoptosis without clear mechanism. However, Important concern need to considered in the development of tumors is protein-coding invasion, migration, and adhesion (IMA) of tumor cells. This study aims at investigating (1) Antiproliferation activities of supernatan yogurt LBA-ST (SY LBA-ST) on zebrafish embryos and HeLa cells, and (2) anti-IMA’s pathway on HeLa cells. The anti-cancer property of SY LBA-ST was evaluated by MTT assay for viability and imunositochemistry of MMP-2, Laminin5-y2, Hsp27, and TGF-β for anti-IMA mechanism. The data is collected and analyzed using One-Way ANOVA.This study could be concluded that (1) SY LBA-ST increase mortality rate and decreased the hatching rate in zebrafish embryos, LC50 ≈ 20% (v/v),  (2) supernatant, pellet, and whole yogurt shows antiproliferation activity on HeLa cells, (3) IC50 SY LBA-ST ≈ 30% (v/v) and the ED = 10% (v/v), and (4) the anti-IMA’s pathway was via decrease MMP-2, Laminin5-y2, Hsp27, and TGF-β expression (p<0.05 ± 0.005).Keywords:  lactobacillus bulgaricus-acidophilus, streptococcus thermophillus, cancer, HeLa, zebrafish
DISTRIBUSI MITOKONDRIA DAN EKSPRESI BAX PADA HUVEC’S YANG MENGALAMI APOPTOSIS AKIBAT INDUKSI OX-LDL Riawan, Wibi; Wibowati, Samodrijanti
Jurnal Kedokteran Brawijaya Vol 22, No 2 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2006.022.02.4

Abstract

ABSTRACT It had been revealed that endothelial dysfunction which is caused by oxydized LDL is related to TNF Receptor Apoptosis Including Ligand (TRAIL) mechanism. However, there is study to see the involvement of mitochondrial mecanism in Ox-LDL endothelial cells dysfunction. This study was done to see the effect of Ox-LDL treatment on apoptosis of HUVEC?s culture  whether  associated  with  the  distribution  of  mitochondria  or  BAX  expression,  the  main  protein  mitochondria apoptosis mechanism. HUVEC?s culture was divided into two groups: control group and treatment group ( 50 mg/ml Ox-LDL for 24 hours). Doublestaining for DNA fragmented (TUNEL) and BAX expression were done respectively, as well as immunochemistry  using  monoclonal  Ab  of  mitochondria.  In  the  control  group  mitochondria  was  distributed  firmly  on cytoplasma of endothelial cells. In contrast, mitochondria were only found near nuclear membrane cells on treatment group  (this  future  usually  found  on  divided  cells) there  was  also  an  increasing  of  BAX  expression  and  a  mount  of apoptotic cells on treatment group compared with control group. It can be concluded that mitochondria is involved in apoptosis of Ox-LDL endothelial cells in order to serve the abundant energy. Keywords: Mitochondria distribution, Bax expresson, Apoptotic, Ox-LDL
ANALISIS LOSS OF HETEROZYGOSITYGEN RAR β2 PADA PATOGENESIS KANKER PAYUDARA Muliartha, I Ketut Gede; Riawan, Wibi; Hidayat, Asnah; Armania, Fitri
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2008.024.02.5

Abstract

The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (RAR ?2) gene on the chromosom 3p24 in the pathogenesis ofbreast cancer. The breast cancer is the most malignant tumor that leads the second cause of death among the female. The recent concept of the theory of pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressorgene, and growth promoting factor gene. However, the diagnostic and therapy of breast cancer are still not satisfied. The lost or decreased in the expressionof RAR  ?2gene on 3p24 chromosome via loss of heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is by experimental and observational. Human female breastcancer and blood samples were gained from public hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident in 3p24 chromosome was detected by PCR and Chromogenic in situ Hybridization (CISH) method.
KEMAMPUAN DARI LACTOBACILLUS PLANTARUM GALUR IS-10506 DAN IS-20506 DALAM MENGHAMBAT AKTIVASI NFKB, MEREGULASI TURUN TNF-RECEPTOR 1 (TNF-R1) DAN OPOPTOSIS PADA BRUSH SEL EPITEL BORDER RATTUS NOVERGICUS YANG DIINDUKSI LPS Kusuma, Titis Sari; Riawan, Wibi; Gunadi Ranuh, I Gusti Made Reza; S. Surono, Ingrid
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2008.024.01.3

Abstract

Probiotic bacteria have a beneficial effect on diarrhea. In this study, we have examined effect of Lactobacillus plantarum Strain IS-10506 (LIS-10506) and IS-20506  (LIS-20506) on inhibition of NF?B activation, downregulation TNF Receptor-1 (TNF-R1) and Apoptosis in Epithelial Brush Border of Rattus novergicus induced with LPS. On the first day, LPS was inducedto Rattus novergicus per oral. At the 3rdday, Lactobacillus plantarum Strain IS-10506 and LIS20506 separately were supplemented for 7 days (the 3rdday up to the 9thday). Rat was sacrificed after anaesthetizing with ether to assess NF?B activation, TNFR1 and apoptosis. Result showed the decreases of activation NF?B in LIS-10506 group as well as in LIS-20506 group, significantly different, in NF?B activation at group with only LPS (average 14.33+4.509) compared to group with LIS-10506 induction (average 2.00+1.732)and LIS-20506 induction (average 1.33+1.528), at p( p<0.000). Downregulation of TNF-R1 was significant  at LPS group compared to LIS-10506 induction as well as LIS-20506 induction. The index of apoptosis showed significant of degradation (p<0.000) after induced by LIS, where LPS group (14.67+2.517), LIS-10506 induction (6.33+2.309) and LIS-20506 induction (6.00+3.000). As a conclusion, supplementation of LIS-10506 and LIS-20506 separately will inhibit theNF?B activation, and although the mechanism was not sure, what by significant degrade the expression TNF-R1( as ekuivalen of activity TNF?), and the mentioned give the implication that happened by the degradation of occurence apoptosis at cell of epitel bush border intestine.
HUBUNGAN ANTARA DERAJAT OBSTRUKSI SERANGAN AKUT ASMA DENGAN KADAR IGE, IFN- DAN IL-4 TOTAL SERUM Kusuma, HMS. Chandra; Kalim, Kusworini Handono; Riawan, Wibi; Muid, Masdar
Jurnal Kedokteran Brawijaya Vol 20, No 2 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2004.020.02.4

Abstract

ABSTRACT Acute bronchial asthma is a common medical emergency the world over. There now exists compelling evidence of a role for Th2-Th1 paradigm in the pathogenesis of asthma in children. We hypothesized that childhood asthma is associated with the activation of  Th2-Th1 lymphocytes whose products regulate at least in part, the expression of IgE, IFN-? and IL-4 and thereby disease severity. Our aims, therefore were to compare the level of total IgE, total IL-4 and total IFN-? in serum from asthmatic and non asthmatic control children matched for age and sex, and to attempt to correlate the IgE, IL-4, and IFN-? total level of serum in the asthmatics children with disease severity. Fifty one children with acute asthma exacerbation were compare with thirty one no asthmatics normal children matched for age and sex. The level of IgE, IL-4, and IFN-? were measured using Elisa. Asthma severity was assessed by a symptom score and spirometri. The level of IgE and Il-4 in the serum of the asthma were elevated as compared to the non asthmatic normal controls, whereas the IFN-? was decreased. There was a significant correlation between elevated levels of IgE and IL-4 with the acute asthma exacerbation severity, whereas the decreased level of IFN-? was not. Conclusions. The increased levels of IgE , IL-4 and the ratio of  IL-4/IFN-? play a crucial role in the acute asthma exacerbation severity.
CYCLOOXYGENASE-2 (COX-2) EXPRESSION ON TESTIS CONNECTIVE TISSUE OF RATTUS NORVEGICUSAFTER TREATMENT WITH BETEL NUT EXTRACT (ARECA CATECHU) Akmal, Muslim; Riawan, Wibi
Jurnal Kedokteran Brawijaya Vol 23, No 3 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2007.023.03.3

Abstract

Betel nut contains alcaloids such as arecoline, arecaine, arecaidine, arecolidine, guvacine, guvacoline and isoguvasine. Arecoline has ability to change gonad morph-function, including shape abnormality of sperm. This research was conducted to prove the ability of betel nut extract (Areca catechu) in causing apoptosis on testis connective tissue of Rattus novergicus. This research used male; 2-3 months age, 150-200 grams body weight of white rats Rattus norvegicus strain Wistar. The rats were divided into 5 groups in equalnumber, 3 rats respectively. They were a control group without treatment and 4 groups as treatment groups which were given doses of betel nut extract, i,e., 1, 2, 3  and 4 gram during seven days. The result showed that dose variation ofbetel nut extract could induction of COX-2 expression on rats (Rattus norvegicus) strain Wistar testicular seminiferous tubule. Keywords: Betel nut extract, Rattus norvegicus, testis, cyclooxygenase-2
PENINGKATAN EKSPRESI LAMININ NAMUN TIDAK VE-CADHERIN PADA SAWAR DARAH OTAK SETELAH INFEKSI Mycobacterium tuberculosis INTRAPULMONALIS Widayati, Aris; Wulandari, Laksmi; Riawan, Wibi
Majalah Kesehatan FKUB Vol 5, No 3 (2018): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.512 KB)

Abstract

Tuberkulosis merupakan  masalah kesehatan utama di dunia. Pada tahun 2016, WHO menemukan angka kejadian TB kurang lebih 10,4 juta, dan untuk Indonesia dilaporkan sebesar 156.723 kasus. Meskipun penyebaran Mycobacterium tuberculosis di susunan saraf pusat tercatat hanya 1%, namun memiliki tingkat kecacatan dan kematian yang tinggi, sehingga menuntut adanya tatalaksana yang efektif untuk mengatasinya. VE-chaderin dan laminin merupakan protein adhesin yang berfungsi mengendalikan permeabilitas pembuluh darah dan mempertahankan integritas blood brain barrier, sehingga kedua protein adhesin tersebut dapat menjadi salah satu target terapi tuberkulosis otak. Penelitian ini bertujuan untuk mengetahui efek paparan M. tuberculosis secara inhalasi terhadap ekspresi laminin dan VE-chaderin pada sel endotel blood brain barrier. Penelitian ini menggunakan mencit Balb/c  (Mus musculus) yang diinfeksi oleh M.tuberculosis strain H37Rv secara inhalasi. Jaringan otak diperiksa menggunakan metode imunohistokimia dengan  antibodi mt-38, antibodi VE-chaderin dan laminin. Hasil penelitian menunjukkan adanya invasi M. tuberculosis pada  mikroglia jaringan otak mencit, diiketahui juga adanya peningkatan ekspresi laminin, sedangkan VE-chaderin tidak menunjukkan adanya perubahan. Proses masuknya M. tuberculosis ke otak diduga terjadi melalui proses diapedesis atau melalui peningkatan ekspresi laminin tanpa perubahan pada VE-chaderin dan reseptor laminin diduga sebagai tempat berikatan yang memungkinkan bakteri tersebut masuk ke jaringan otak. 
Analysis of the Histopathology, TNF-α of Microglia Cells Expression, NRG-1/erbB Oligodendrocyte, and Ki67/Apoptosis of Dentate Gyrus Rattus novergicus Brain After Acute Traumatic Brain Injury Riawan, Wibi; Alfiantya, Putri Fitri; Adianingsih, Oktavia Rahayu; Zulkarnaen, Zulkarnaen; Jazmi, Alif Fariz; Hemas N., Sitti Ayu
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp20-29

Abstract

Head trauma or traumatic brain injury (TBI) gives most serious impact on the central nervous system. Several experimental models have been established to mimic different pathogenesis characteristics of TBI. The purpose of this study was to determine whether there is evidence of hystopathological lesions in the brain tissue after Marmorou TBI models. This study uses Rattus norvegicus Sprague Dawley strain. Macroscopic and microscopic observations on the brain tissue were done. Macroscopic lesions were observed in the brain. Microscopic observation was performed with Haematoxylin-Eosin (HE) staining and immunohistochemistry on the distribution of microglia cells and pyramidal cells in the cortex. Meanwhile, the distribution of NRG-1/ErbB, proliferation, and apoptosis were observed in the hippocampus. The results of macroscopic observation showed that there were wounds caused by falling loads and vasodilatation. On microscopic observation, the TBI group showed an increase in neutrophils distribution and distribution of activated microglia to produce TNF-α, and decrease in the number of cortical pyramidal cells significantly. The distribution of NRG-1 tended to decrease after exposure of TBI and had no effect on its receptor, erbB. Exposure of TBI appears to lower the activity of neuronal cells proliferation in dentate gyrus (DG) area and significantly increase the number of apoptotic cells. Marmarou model is a physiological model of TBI that spontaneously occurs following a trauma to the head, for example trauma due to an accident. This data can be used as a preliminary data of inflammation and tissue regeneration of disrupted adult brain. Therefore, this research could be used as the basis in the studies of therapeutic agents in the process of neurogenesis of brain cells.Keywords: traumatic brain injury, ERG-1/ErbB, dentate gyrus, Ki67, TNF-a, microglia